229 research outputs found
Effects of Antibiotics on the Intestinal Microbiota of Mice
Studies on human and mouse gastrointestinal microbiota have correlated the composition of the microbiota to a variety of diseases, as well as proved it vital to prevent colonization with resistant bacteria, a phenomenon known as colonization resistance. Antibiotics dramatically modify the gut community and there are examples of how antibiotic usage lead to colonization with resistant bacteria [e.g., dicloxacillin usage selecting for ESBL-producing E. coli carriage], as shown by Hertz et al. Here, we investigated the impact of five antibiotics [cefotaxime, cefuroxime, dicloxacillin, clindamycin, and ciprofloxacin] on the intestinal microbiota in mice. Five different antibiotics were each given to groups of five mice. The intestinal microbiotas were profiled by use of the IS-pro analysis; a 16S–23S rDNA interspace [IS]-region-based profiling method. For the mice receiving dicloxacillin and clindamycin, we observed dramatic shifts in dominating phyla from day 1 to day 5. Of note, diversity increased, but overall bacterial load decreased. For ciprofloxacin, cefotaxime, and cefuroxime there were few overall changes. We speculate that antibiotics with efficacy against the abundant anaerobes in the gut, particularly Bacteroidetes, can in fact be selected for resistant bacteria, disregarding the spectrum of activity
Glassy Transition and Aging in a Model without Disorder
We study the off-equilibrium relaxational dynamics of the Amit-Roginsky
field theory, for which the mode coupling approximation is exact. We
show that complex phenomena such as aging and ergodicity breaking are present
at low temperature, similarly to what is found in long range spin glasses. This
is a generalization of mode coupling theory of the structural glass transition
to off-equilibrium situations.Comment: 9 pages, 1 uuencoded figure, LaTex, preprint NORDITA 94/3
Air Pollution and Lymphocyte Phenotype Proportions in Cord Blood
Effects of air pollution on morbidity and mortality may be mediated by alterations in immune competence. In this study we examined short-term associations of air pollution exposures with lymphocyte immunophenotypes in cord blood among 1,397 deliveries in two districts of the Czech Republic. We measured fine particulate matter < 2.5 μm in diameter (PM(2.5)) and 12 polycyclic aromatic hydrocarbons (PAHs) in 24-hr samples collected by versatile air pollution samplers. Cord blood samples were analyzed using a FACSort flow cytometer to determine phenotypes of CD3(+) T-lymphocytes and their subsets CD4(+) and CD8(+), CD19(+) B-lymphocytes, and natural killer cells. The mothers were interviewed regarding sociodemographic and lifestyle factors, and medical records were abstracted for obstetric, labor and delivery characteristics. During the period 1994 to 1998, the mean daily ambient concentration of PM(2.5) was 24.8 μg/m(3) and that of PAHs was 63.5 ng/m(3). In multiple linear regression models adjusted for temperature, season, and other covariates, average PAH or PM(2.5) levels during the 14 days before birth were associated with decreases in T-lymphocyte phenotype fractions (i.e., CD3(+) CD4(+), and CD8(+)), and a clear increase in the B-lymphocyte (CD19(+)) fraction. For a 100-ng/m(3) increase in PAHs, which represented approximately two standard deviations, the percentage decrease was −3.3% [95% confidence interval (CI), −5.6 to −1.0%] for CD3(+), −3.1% (95% CI, −4.9 to −1.3%) for CD4(+), and −1.0% (95% CI, −1.8 to −0.2%) for CD8(+) cells. The corresponding increase in the CD19(+) cell proportion was 1.7% (95% CI, 0.4 to 3.0%). Associations were similar but slightly weaker for PM(2.5). Ambient air pollution may influence the relative distribution of lymphocyte immunophenotypes of the fetus
Domestication alone does not lead to inequality: intergenerational wealth transmission among horticulturalists
We present empirical measures of wealth inequality and its intergenerational transmission among four horticulturalist populations. Wealth is construed broadly as embodied somatic and neural capital, including body size, fertility and cultural knowledge, material capital such as land and household wealth, and relational capital in the form of coalitional support and field labor. Wealth inequality is moderate for most forms of wealth, and intergenerational wealth transmission is low for material resources and moderate for embodied and relational wealth. Our analysis suggests that domestication alone does not transform social structure; rather, the presence of scarce, defensible resources may be required before inequality and wealth transmission patterns resemble the familiar pattern in more complex societies. Land ownership based on usufruct and low‐intensity cultivation, especially in the context of other economic activities such as hunting and fishing, is associated with more egalitarian wealth distributions as found among hunter‐gatherers
Randomly Crosslinked Macromolecular Systems: Vulcanisation Transition to and Properties of the Amorphous Solid State
As Charles Goodyear discovered in 1839, when he first vulcanised rubber, a
macromolecular liquid is transformed into a solid when a sufficient density of
permanent crosslinks is introduced at random. At this continuous equi- librium
phase transition, the liquid state, in which all macromolecules are
delocalised, is transformed into a solid state, in which a nonzero fraction of
macromolecules have spontaneously become localised. This solid state is a most
unusual one: localisation occurs about mean positions that are distributed
homogeneously and randomly, and to an extent that varies randomly from monomer
to monomer. Thus, the solid state emerging at the vulcanisation transition is
an equilibrium amorphous solid state: it is properly viewed as a solid state
that bears the same relationship to the liquid and crystalline states as the
spin glass state of certain magnetic systems bears to the paramagnetic and
ferromagnetic states, in the sense that, like the spin glass state, it is
diagnosed by a subtle order parameter.
In this review we give a detailed exposition of a theoretical approach to the
physical properties of systems of randomly, permanently crosslinked
macromolecules. Our primary focus is on the equilibrium properties of such
systems, especially in the regime of Goodyear's vulcanisation transition.Comment: Review Article, REVTEX, 58 pages, 3 PostScript figure
Break up of heavy fermions at an antiferromagnetic instability
We present results of high-resolution, low-temperature measurements of the
Hall coefficient, thermopower, and specific heat on stoichiometric YbRh2Si2.
They support earlier conclusions of an electronic (Kondo-breakdown) quantum
critical point concurring with a field induced antiferromagnetic one. We also
discuss the detachment of the two instabilities under chemical pressure. Volume
compression/expansion (via substituting Rh by Co/Ir) results in a
stabilization/weakening of magnetic order. Moderate Ir substitution leads to a
non-Fermi-liquid phase, in which the magnetic moments are neither ordered nor
screened by the Kondo effect. The so-derived zero-temperature global phase
diagram promises future studies to explore the nature of the Kondo breakdown
quantum critical point without any interfering magnetism.Comment: minor changes, accepted for publication in JPS
Abrogation of chronic rejection in a murine model of aortic allotransplantation by prior induction of donor-specific tolerance
Aortic allotransplantation in mice has been well established as a model of choice to study the evolvement of chronic rejection, the etiopathology of which is believed to be that of immune origin. This has prompted the postulation that prior induction of donor-specific tolerance would attenuate or abrogate the underlying events that culminate in posttransplant arteriosclerosis. To study the effects of donor-specific tolerance on chronic rejection, we performed orthotopic liver transplantation without immunosuppression in mice 30 days before aortic allotransplantation across C57Bl/10J (H2b)→C3H (H2(k)) strain combinations (group III). Aortic allografting in syngeneic (group I; C3H→C3H) and allogeneic (group II, C57Bl/10J→C3H) animals served as controls. No morphological changes were evidenced in the transplanted aortas in group I animals. Contrarily, aortic allografts in group H animals underwent a self-limiting acute cellular rejection, which resolved completely and was succeeded by day 30 after transplantation by histopathological changes pathognomonic of chronic rejection. There was evidence for diffuse myointimal thickening, progressive concentric luminal narrowing, and patchy destruction of internal elastic membranes resulting in massive vascular obliteration by day 120 after transplantation. It was of interest that no arteriosclerotic changes were observed for the duration of follow-up (up to 120 days after transplantation) in transplanted aortas (liver donor-type) harvested from animals in group III. However, vasculopathy was prominent in third-party aortic grafts transplanted into tolerant recipients. Taken together, these data suggest that prior induction of tolerance abrogates the development of chronic rejection; this protection seems to be donor specific
The genome sequence of <i>Trypanosoma brucei gambiense</i>, causative agent of chronic Human African Trypanosomiasis
<p><b>Background:</b> <i>Trypanosoma brucei gambiense</i> is the causative agent of chronic Human African Trypanosomiasis or sleeping sickness, a disease endemic across often poor and rural areas of Western and Central Africa. We have previously published the genome sequence of a <i>T. b. brucei</i> isolate, and have now employed a comparative genomics approach to understand the scale of genomic variation between <i>T. b. gambiense</i> and the reference genome. We sought to identify features that were uniquely associated with <i>T. b. gambiense</i> and its ability to infect humans.</p>
<p><b>Methods and findings:</b> An improved high-quality draft genome sequence for the group 1 <i>T. b. gambiense</i> DAL 972 isolate was produced using a whole-genome shotgun strategy. Comparison with <i>T. b. brucei</i> showed that sequence identity averages 99.2% in coding regions, and gene order is largely collinear. However, variation associated with segmental duplications and tandem gene arrays suggests some reduction of functional repertoire in <i>T. b. gambiense</i> DAL 972. A comparison of the variant surface glycoproteins (VSG) in <i>T. b. brucei</i> with all <i>T. b. gambiense</i> sequence reads showed that the essential structural repertoire of VSG domains is conserved across <i>T. brucei</i>.</p>
<p><b>Conclusions:</b> This study provides the first estimate of intraspecific genomic variation within <i>T. brucei</i>, and so has important consequences for future population genomics studies. We have shown that the <i>T. b. gambiense</i> genome corresponds closely with the reference, which should therefore be an effective scaffold for any <i>T. brucei</i> genome sequence data. As VSG repertoire is also well conserved, it may be feasible to describe the total diversity of variant antigens. While we describe several as yet uncharacterized gene families with predicted cell surface roles that were expanded in number in <i>T. b. brucei</i>, no <i>T. b. gambiense</i>-specific gene was identified outside of the subtelomeres that could explain the ability to infect humans.</p>
Frustration and the Kondo effect in heavy fermion materials
The observation of a separation between the antiferromagnetic phase boundary
and the small-large Fermi surface transition in recent experiments has led to
the proposal that frustration is an important additional tuning parameter in
the Kondo lattice model of heavy fermion materials. The introduction of a Kondo
(K) and a frustration (Q) axis into the phase diagram permits us to discuss the
physics of heavy fermion materials in a broader perspective. The current
experimental situation is analysed in the context of this combined "QK" phase
diagram. We discuss various theoretical models for the frustrated Kondo
lattice, using general arguments to characterize the nature of the -electron
localization transition that occurs between the spin liquid and heavy Fermi
liquid ground-states. We concentrate in particular on the Shastry--Sutherland
Kondo lattice model, for which we establish the qualitative phase diagram using
strong coupling arguments and the large- expansion. The paper closes with
some brief remarks on promising future theoretical directions.Comment: To appear in a special issue of JLT
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