Contribution for new genetic markers of rheumatoid arthritis activity and severity : sequencing of the tumor necrosis factor-alpha gene promoter

© 2007 Fonseca et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly citedThe objective of this study was to assess whether clinical measures of rheumatoid arthritis activity and severity were influenced by tumor necrosis factor-alpha (TNF-alpha) promoter genotype/haplotype markers. Each patient's disease activity was assessed by the disease activity score using 28 joint counts (DAS28) and functional capacity by the Health Assessment Questionnaire (HAQ) score. Systemic manifestations, radiological damage evaluated by the Sharp/van der Heijde (SvdH) score, disease-modifying anti-rheumatic drug use, joint surgeries, and work disability were also assessed. The promoter region of the TNF-alpha gene, between nucleotides -1,318 and +49, was sequenced using an automated platform. Five hundred fifty-four patients were evaluated and genotyped for 10 single-nucleotide polymorphism (SNP) markers, but 5 of these markers were excluded due to failure to fall within Hardy-Weinberg equilibrium or to monomorphism. Patients with more than 10 years of disease duration (DD) presented significant associations between the -857 SNP and systemic manifestations, as well as joint surgeries. Associations were also found between the -308 SNP and work disability in patients with more than 2 years of DD and radiological damage in patients with less than 10 years of DD. A borderline effect was found between the -238 SNP and HAQ score and radiological damage in patients with 2 to 10 years of DD. An association was also found between haplotypes and the SvdH score for those with more than 10 years of DD. An association was found between some TNF-alpha promoter SNPs and systemic manifestations, radiological progression, HAQ score, work disability, and joint surgeries, particularly in some classes of DD and between haplotypes and radiological progression for those with more than 10 years of DD.This work was supported by grant POCTI/SAU-ESP/59111/2004 from Fundação Ciência e Tecnologia.info:eu-repo/semantics/publishedVersio

The international EAACI/GA²LEN/EuroGuiDerm/APAAACI guideline for the definition, classification, diagnosis, and management of urticaria

This update and revision of the international guideline for urticaria was developed following the methods recommended by Cochrane and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) working group. It is a joint initiative of the Dermatology Section of the European Academy of Allergology and Clinical Immunology (EAACI), the Global Allergy and Asthma European Network (GA(2)LEN) and its Urticaria and Angioedema Centers of Reference and Excellence (UCAREs and ACAREs), the European Dermatology Forum (EDF; EuroGuiDerm), and the Asia Pacific Association of Allergy, Asthma and Clinical Immunology with the participation of 64 delegates of 50 national and international societies and from 31 countries. The consensus conference was held on 3 December 2020. This guideline was acknowledged and accepted by the European Union of Medical Specialists (UEMS). Urticaria is a frequent, mast cell-driven disease that presents with wheals, angioedema, or both. The lifetime prevalence for acute urticaria is approximately 20%. Chronic spontaneous or inducible urticaria is disabling, impairs quality of life, and affects performance at work and school. This updated version of the international guideline for urticaria covers the definition and classification of urticaria and outlines expert-guided and evidence-based diagnostic and therapeutic approaches for the different subtypes of urticaria

The international EAACI/GA(2)LEN/EuroGuiDerm/APAAACI guideline for the definition, classification, diagnosis, and management of urticaria

Publisher Copyright: © 2021 GA²LEN. Allergy published by European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd.This update and revision of the international guideline for urticaria was developed following the methods recommended by Cochrane and the Grading of Recommendations Assessment, Development and Evaluation (GRADE) working group. It is a joint initiative of the Dermatology Section of the European Academy of Allergology and Clinical Immunology (EAACI), the Global Allergy and Asthma European Network (GA(2)LEN) and its Urticaria and Angioedema Centers of Reference and Excellence (UCAREs and ACAREs), the European Dermatology Forum (EDF; EuroGuiDerm), and the Asia Pacific Association of Allergy, Asthma and Clinical Immunology with the participation of 64 delegates of 50 national and international societies and from 31 countries. The consensus conference was held on 3 December 2020. This guideline was acknowledged and accepted by the European Union of Medical Specialists (UEMS). Urticaria is a frequent, mast cell-driven disease that presents with wheals, angioedema, or both. The lifetime prevalence for acute urticaria is approximately 20%. Chronic spontaneous or inducible urticaria is disabling, impairs quality of life, and affects performance at work and school. This updated version of the international guideline for urticaria covers the definition and classification of urticaria and outlines expert-guided and evidence-based diagnostic and therapeutic approaches for the different subtypes of urticaria.Peer reviewe

estudos artísticos

info:eu-repo/semantics/publishedVersio

Imagens interditas? : limites e rupturas em representações explícitas do sexo no pós-25 de Abril

Tese de doutoramento, Belas-Artes (Pintura), Universidade de Lisboa, Faculdade de Belas-Artes, 2014Esta investigação teórica, bem como prática, é estruturada na análise de representações sexualmente explícitas no território das artes plásticas, quando associadas a conteúdos considerados obscenos e/ ou pornográficos, em interpretações controversas entre o socialmente admitido e o ilícito, configurando uma temática reconhecida como provocatória e particularmente transgressora ao exceder a erotica comum. Tem origem num contexto plástico experimental e provocatório, apresentado na Galeria Quadrum, em Lisboa, com efeitos reactivos no tecido social e nos processos criativos individuais. É uma pesquisa direccionada para o debate referente às polémicas que geram interdições, censuras ou processos de autocensura, em projectos visuais desta natureza e com acesso público, procurando interpretar no território português manifestações artísticas neste sentido, incorporando influências e referências exteriores, incontornáveis nos processos conceptuais da investigação plástica experimental. O século XX, particularmente nos últimos 25 anos, apresenta paradoxalmente e de um modo claro, um pudor público na fruição de representações artísticas da sexualidade, oculto nas liberdades que a evolução moral, política e social anunciam, revelando limites e gerando rupturas visuais significativas, na conquista do direito à liberdade de expressão. O contexto nacional permite um reduzido conjunto de obras plásticas e sexualmente explícitas no território nacional, após o 25 de Abril de 1974 e que se estende até ao presente, o que possibilita agora, passadas quatro décadas, um olhar e um afastamento que se pretende imparcial, para uma análise da produção artística nesta temática tão controversa, realizada à luz das conquistas sociais estruturadas neste período.This theoretical research, as well by project, is structured upon the analysis of sexually explicit depictions in the fine arts territories, setting up a theme field often recognized as particularly provocative and transgressive, as it exceeds the ordinary erotica and allow associations to contents deemed obscene and/ or pornographic, as well controversial interpretations between the socially accepted and the illicit. Arises from an experimental and provocative artistic context, shown in Quadrum Gallery, in Lisbon, with reactive effects in social grounds and individual creative processes. It is targeted to the debate concerning the controversies that generate prohibitions, censorship or self-censorship processes in visual projects of this nature and with public access, examining interpretations in the Portuguese territory of artistic expressions in this effect, incorporating influences and outdoor unavoidable references in the conceptual processes of experimental plastic research. The twentieth century, mostly in its last 25 years, paradoxically presents an embarrassment in the delight of seeing the artistic representation of sexuality, hidden in the freedoms that the moral, political and social changes announced, revealing limits and generating significant visual disruptions in the accomplishment of the right to freedom of expression. The national context allows a reduced set of fine arts and sexually explicit accomplishments within the national territory, after April 25th of 1974, extending to the present, making it possible now, after four decades, a look and a distance that is intended impartial, for an analysis of the artistic production in this very controversial subject, held in the light of the structured social achievements in this period

Flow cytometry TUNEL standardization for assaying sperm DNA fragmentation

We read with great interest Muratori's paper (Muratori et al, 2010), because we believe that terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling (TUNEL) assay standardization is a matter of great concern and that there are certain steps that must be followed to achieve accurate results.Fil: Curi, Susana M.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Chenlo, Patricia H.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Billordo, Luis Ariel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Baz, Placida. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sardi, Melba L.. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Ariagno, Julia Irene. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Repetto, Herberto. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; ArgentinaFil: Mendeluk, Gabriela Ruth. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pugliese, Mercedes Norma. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Bioquímica Clínica; Argentin