The 68 kDa protein of signal recognition particle contains a glycine-richregion also found in certain RNA-binding proteins

Signal recognition particle (SRP) interacts with the signal sequence in nascent secretory and membrane proteins and directs them to the membrane of the endoplasmic reticulum. Membrane targeting is mediated by the 68 and the 72 kDa proteins of SRP. We have cloned and sequenced cDNA encoding the 68 kDa protein of canine signal recognition particle (SRP68). SRP68 is a basic protein comprised of 622 amino acid residues. Close to the amino terminus there is a glycine-rich region which SRP68 has in common with some RNA-binding proteins. SRP68 shares no detectable similarity to any of the proteins in data libraries

Novel prognostic factors in laryngeal carcinoma patients

Hintergrund. Die Behandlungsmöglichkeiten des Larynxkarzinoms umfassen Strahlentherapie, offen Chirurgie und transorale Lasermikrochirurgie (TLM). Die derzeitige Studienlage zeigt ähnliches Überleben unabhängig von der Behandlungsmethode. Wir untersuchten das klinische Ergebnis von Patienten mit glottischen und supraglottischen Plattenepithelkarzinomen, welche mit TLM an der Universitätsklinik für Hals-, Nasen- und Ohrenheilkunde an der Medizinischen Universität Wien zwischen 1994 und 2012 behandelt wurden. Das „engulfment and cell motility” Protein 3 wurde mit Tumorgröße, Stadium und der Metastasierung von nicht-kleinzelligen Lungenkarzinomen beschrieben. Bisher wurde dies allerdings noch nicht bei Kopf/Hals - Malignomen erforscht. Wir untersuchten daher seine prognostische Relevanz für das Überleben in Patienten mit frühen glottischen Larynxkarzinomen. Methoden. Wir führten eine retrospektive Analyse von an unserer Klinik mit TLM erstbehandelten Patienten durch. Aus der gesamten Studienkohorte wurden Patienten mit T1N0M0 glottischen Karzinomen identifiziert und die histologischen Paraffin fixierten Proben untersucht. Es wurden immunhistochemische Färbungen für das ELMO3 Protein in dem Primärtumor durchgeführt und mit den klinischen Daten korreliert. Ergebnisse. Insgesamt 112 Patienten (37 supraglottische und 75 glottische) wurden mit einer medianen Nachbeobachtungszeit von 92 Monaten eingeschlossen. Das mittlere Alter bei der Behandlung waren 61 Jahre. Das 5- und 10-Jahres Gesamtüberleben war 75% und 50%, während das 5- und 10-Jahres krankheitsfreie Überleben 60% und 40%. Betrug. 23% der 48 glottischen Karzinompatienten waren 8 ELMO3 positiv, welches mit schlechten krankheitsspezifischen und krankheitsfreien Überleben (p <0,05) korrelierte. Zusammenfassung. Lokoregionale Kontrolle, das krankheitsfreie Überleben und das Gesamtüberleben in unserer Studie waren vergleichbar mit der aktuellen Literatur. Des Weiteren konnte die Expression von ELMO3 als prognostischer Faktor bei Patienten mit frühem glottischen Larynxkarzinom identifiziert werden.Background. Transoral laser microsurgery (TLM) has become a standard alternative treatment to radiotherapy and open surgery in patients with laryngeal cancer. Currently available data does not distinctly favor one treatment modality over the other. We aimed to assess the clinical outcome in patients with glottic and supraglottic squamous cell carcinoma treated with TLM at the Department of Otorhinolaryngology, Head and Neck Surgery at the Medical University of Vienna between 1994 and 2012. The engulfment and cell motility 3 (ELMO3) protein expression has been correlated with tumor size, TNM stage, lymph node metastases and distant metastases in non-small cell lung cancer, but has not been investigated in head and neck cancers. We therefore aimed to identify its prognostic relevance in patients with early glottic cancer. Methods. We conducted a retrospective medical chart review of previously untreated glottic and supraglottic squamous cell carcinoma patients who underwent transoral laser microsurgery. Out of this study cohort, patients with T1N0M0 glottic cancer were identified and histologic paraffin-embedded specimens retrieved. Immunohistochemistry staining for ELMO3 was performed in the primary tumor and correlated with clinical outcome. Results. Overall 112 patients (37 supraglottic and 75 glottic) were included with a median follow-up time of 92 months. The median age at treatment was 61 years. The 5- and 10-year overall survival was 75% and 50%, whereas the 5- and 10-year disease-free survival was 60% and 40%. In 48 glottic cancer patients ELMO3 expression was positive in 23% of the specimen, which was correlated with poor disease-specific and disease free survival (p < 0.05). Conclusion. Locoregional control, disease-free survival and overall survival in our study population were comparable to current literature. Furthermore ELMO3 might serve as future prognostic biomarker in patients with early glottic cancer.submitted by Dr. med.univ. Georg HaymerleZusammenfassung in deutscher SpracheAbweichender Titel laut Übersetzung der Verfasserin/des VerfassersMedizinische Universität, Dissertation, 2017OeB

Risk factors for acute unplanned tracheostomy during panendoscopy in HNSCC patients.

BackgroundDespite of careful pre-operative risk evaluation some patients require an acute unplanned tracheostomy during panendoscopy.MethodsRisk factors of patients requiring an unplanned tracheostomy during panendoscopy (n = 32) were compared to a control group with panendoscopy without tracheostomy (n = 180).Results2131 panendoscopies for Head and Neck squamous cell carcinoma were performed at our Department between 2000 and 2014. Unplanned tracheostomies were necessary in 1.6% of all panendoscopies. Patients with laryngeal cancer (p = 0.001) or abnormal activated partial thromboplastin time (aPTT) (p = 0.03) had a statistically significant higher risk of unplanned tracheostomy. Regression analysis showed that patients with advanced laryngeal cancer had an almost 6 times higher risk for tracheostomy than patients with early stage oropharyngeal cancer.ConclusionsWe identified abnormal aPTT and laryngeal carcinoma as significant predictors for unplanned tracheostomy during panendoscopy. The results of our study could improve preoperative risk evaluation in HNSCC patients

Bilateral stenting of symptomatic and asymptomatic internal carotid artery stenosis due to fibromuscular dysplasia

Stent grafting of internal carotid artery (ICA) stenoses due to fibromuscular dysplasia has been rarely and only unilaterally carried so far. Bilateral carotid stent grafting of ICA stenoses due to fibromuscular dysplasia has not been reported previously. In a 37 year old woman with recurrent right hemispheric transitory ischaemic attacks, a non-disabling minor stroke, and recurrent right amaurosis fugax despite antithrombotic therapy, cerebral angiography disclosed a long segment narrowing, distal, high grade (95%) stenosis of the right ICA and a long narrowing, distal high grade (70%) stenosis of the left ICA. Morphological features of both stenoses were indicative of fibromuscular dysplasia. The right sided stenosis was stented with a PTFE-HEMOBAHN® endoprosthesis; this was followed by a brief, postprocedural left sided hemiparesis. The left sided ICA stenosis was successfully stented by the same procedure. Nine months later, both stents were still patent and the patient was symptom free. Bilateral carotid stenting may remain an alternative to endarterectomy in bilateral ICA stenosis due to fibromuscular dysplasia when ischaemic events persist despite full antithrombotic therapy.