Pacific 187Os/188Os isotope chemistry and U-Pb geochronology: synchroneity of global Os isotope change across OAE 2

Studies of OAE 2 sections beyond the Atlantic Ocean, Western Interior Seaway (WIS) and European pelagic shelf are limited. Here, we present initial osmium isotope stratigraphy (187Os/188Os–Osi) from two proto-Pacific sites that span the Cenomanian–Turonian boundary interval (CTBI): the Yezo Group (YG) section, Hokkaido, Japan, and the Great Valley Sequence (GVS), California, USA; to evaluate the 187Os/188Os seawater chemistry of the proto-Pacific. Additionally we combine new 206Pb/238U zircon CA-ID-TIMS geochronology from five volcanic tuff horizons of the Yezo Group section to test and facilitate inter-basinal integration with the WIS using radio-isotopically constrained age–depth models for both sections, and quantitatively constrain the absolute timing and duration of events across the CTBI. The YG shows an almost identical Osi profile to that of the WIS, and very similar to that of other sites of the proto-Atlantic and European pelagic oceans (Turgeon and Creaser, 2008 and Du Vivier et al., 2014). The characteristics of the Osi profile are radiogenic and heterogeneous (∼0.55–0.85) prior to the OAE 2, and synchronous with the inferred OAE 2 onset the Osi abruptly become unradiogenic and remain relatively homogeneous (∼0.20–0.30) before showing a gradual return to more radiogenic View the MathML source throughout the middle to late OAE 2. A206Pb/238U zircon age of an interbedded tuff (HK017) in the adjacent horizon to the first unradiogenic Osi value constrains the age of the Osi inflection at 94.44±0.14 Ma. This age, including uncertainty, agrees with the interpolated age of the same point in the Osi profile (94.28±0.25 Ma) in the only other dated OAE 2 section, the WIS; indicating a coeval shift in seawater chemistry associated with volcanism at the OAE 2 onset at the levels of temporal resolution (ca. 0.1 Myr). Further, prior to the onset of OAE 2 an enhanced radiogenic inflection in the Osi profile of the YG is correlative, within uncertainty, with a similar trend in the WIS based on the U–Pb age–depth model. The interpolated ages, 94.78±0.12 Ma and 94.66±0.25 Ma for this Osi inflection in the YG and WIS, respectively, indicate that palaeocirculation was sufficient to simultaneously influence transbasinal seawater chemistry. In contrast, the pre-OAE 2 Osi profile for the GVS is disparate to that of the YG and those of the proto-Atlantic and European pelagic shelf locations. We interpret the pre OAE 2 heterogeneous Osi values (0.30–0.95) to record a palaeobasin that was regionally influenced interchangeably by both unradiogenic (hydrothermal flux) and radiogenic (continental flux) Os. We conclude that the Osi profiles from the proto-Pacific sections record both trends that are consistent globally (OAE 2 onset, syn and post OAE 2), but also show regional differences (pre OAE 2) between OAE 2 sections worldwide. As such the Osi profiles coupled with U–Pb geochronology facilitate the correlation of OAE 2 stratigraphy, and demonstrate both regional and global ocean dynamics

Étude de la corrosion du cuivre par microélectrochimie: Comparaison entre microélectrode et microcellule

Ce travail présente la comparaison de mesures électrochimiques réalisées à l'aide d'une microcellule et de microélectrodes conventionnelles. Des mesures de voltamétrie cyclique et d’impédances électrochimiques ont été réalisées sur des électrodes de cuivre pur, électrode classique couplée à des capillaires et microélectrodes, au potentiel de corrosion dans une solution NaCl 0,1 M, La comparaison entre ces deux types de mesure apporte des informations relatives à leurs sensibilités et limitations. En particulier, le domaine des basses fréquences du diagramme d’impédance est sensible à la géométrie de la microcellule ainsi qu'au positionnement relatif des électrodes

Survey of a wastewater treatment plant microfauna by image analysis

The microfauna present in the activated sludge of a wastewater treatment plant is mainly composed by bacteria, protozoa and metazoa. The protozoan species are quite sensitive to physical, chemical and operational processes making them, thus, precious indicators of the state of the plant. Several authors already established relationships between the predominance of certain species or group and some parameters of the plant, such as the biotic indices namely the Sludge Biotic Index. All the above-mentioned procedures demand the identification, classification and quantification of the different species. Normally this is done manually, which implies both time and expertise. In the present work a semi-automatic protozoan recognition procedure by means of image analysis is attempted. The program built for this purpose (ProtoRec v.3) was also used to study the evolution of the microfauna during transient operation times (stoppage and re-run). The results were rather satisfactory in terms of protozoa recognition and the survey of the transient phase allowed verifying the aging and degradation of the microfauna by means of the different predominant species

Estudo por Análise de Imagem do Comportamento de uma Estação de Tratamento de Efluentes sob Condições Transientes

A microfauna presente numa estação de tratamento de efluentes por lamas activadas é composta essencialmente por bactérias, protozoários e metazoários. Os protozoários apresentam uma sensibilidade aos fenómenos físico-químicos e operacionais que os tornam indicadores do estado de funcionamento da estação. A literatura apresenta relações entre a abundância de uma certa espécie ou grupo e parâmetros da estação. Uma outra possibilidade é a qualificação do estado da ETAR através de índices bióticos como o SBI. Todos estes procedimentos exigem uma identificação, classificação e quantificação das espécies presentes. Geralmente isto é feito manualmente, o que implica tempo e muitas vezes a presença de um técnico especializado. Neste trabalho é proposto um procedimento para reconhecimento semi-automático das espécies de protozoários por análise de imagem. Este programa (ProtoRec v.2) foi em seguida utilizado para estudar a evolução da microfauna durante períodos de transição (paragem e arranque da estação). Os resultados obtidos foram satisfatórios em relação ao reconhecimento das espécies pelo programa e o acompanhamento da estação permitiu-nos verificar a degradação e envelhecimento da lama e o aparecimento de protozoários que evidenciam tal facto

Survey of protozoa and metazoa populations in wastewater treatment plants by image analysis and discriminant analysis

The biota present in the activated sludge of a wastewater treatment plant are mainly composed of bacteria, Protozoa and Metazoa. The Protozoa and Metazoa species are important micro-organisms taking part in the ecosystem balance in wastewater treatment plants and are quite sensitive to physical, chemical and operational processes. Several authors have established relationships between the predominance of certain species or groups and some variables of the plant. Such analysis demands the identification and quantification of the different species, which requires skilled workers, specializing in zoology or protozoology, and is time-consuming. To overcome those problems, semi-automatic image analysis and discriminant analysis were carried out for the identification procedures. The overall results were very promising in terms of Protozoa and Metazoa group recognition and of survey of plant conditions. Copyright 2004 John Wiley & Sons, Ltd.Financial support for the co-operation between the Portuguese (Braga) and French (Nancy) teams was provided by the French Embassy in Portugal and ICCTI (Portugal). A. L. Amaral was supported by a PhD grant (PRAXIS XXI/BD/20325/99) of ‘Fundação para a Ciência e Tecnologia’ (Portugal). M. da Motta was supported by a grant of the National Council of Scientific and Technological Development of Brazil (CNPq)

Study on the Performance of Wastewater Treatment Plant by Image Analysis: Validation and Case Study

Um método baseado na análise de imagens foi desenvolvido para caracterizar os flocos de lodo ativado em termos do seu tamanho e forma (dimensão fractal) e da abundância de bactérias filamentosas. Após ter sido testado em experimentos em escala piloto, o método foi validado para estações de tratamento reais. Foram analisadas doze estações de tratamento de esgotos situadas na França e em Portugal, de tamanhos e processos diferentes. Em seguida realizou-se um acompanhamento sobre a estação de Braga (Portugal) que estava com problemas de intumescimento do lodos (“bulking” filamentoso).Automated methods based on image analysis have been developed to characterise activated sludge in terms of size and shape (fractal dimension) of flocs and abundance of filamentous bacteria. After tests on pilot-scale reactors, the method has been validated on samples from twelve different full-scale wastewater treatment plants of different sizes and processes in France and Portugal. Finally sludge morphology changes have been monitored during a bulking event on a full-scale plant in Portugal

Study of protozoa population in wastewater treatment plants by image analysis

Protozoa are important micro-organisms taking part to the ecosystem balance in wastewater treatment plants. A procedure for their semi-automated identification and counting based on image analysis is proposed. The main difficulty is the segmentation of the protozoa as most of them are in contact with the sludge. The protozoa are characterized by the size of their silhouette (area and length) and three shape factors (elongation, circularity and eccentricity). The identification is performed after projecting the resulting 5D space into a 3D space of Principal Components. The rate of automated identification is actually higher than 50% for some of the species found commonly in activated sludge