405 research outputs found
Letter to the editor: Backbone resonance assignment of the homodimeric, 35 kDa chaperone CesT from enteropathogenic Escherichia coli
Abundance and composition of free and aggregate-occluded carbohydrates and lignin in two forest soils as affected by wildfires of different severity
International audienceOrganic matter is the soil component most affected by wildfires, both in terms of abundance and composition. Fire-induced alteration of soil organic matter (SOM) depends on heating intensity and duration, oxygen availability and other factors related to topography, climate, soil and vegetation features. Particularly affected by fire is the litter layer, but SOM from the uppermost mineral soil can also experience some major changes. In this study, we investigated the direct impact of fire on molecular SOM parameters in density fractions isolated from the top 2.5 cm of mineral soil in two forests that recently experienced wildfires of different severity. One, located in Tuscany, Central Italy, is a mixed forest of Downy oak and Maritime pine, developed on Acrisols formed on sandy lacustrine deposits, affected by a moderately severe fire. The other, located in Victoria, South-East Australia, is a mixed-species eucalypt forest, developed on a Cambisol formed on sandy Devonian sediments, affected by an extremely severe fire (the infamous 'Black Saturday' fire). The purpose of this study was the assessment of fire-induced changes on amount and composition of the bulk SOM and SOM associated to soil fractions having different densities. We used 1.8 Mg m(-3) as density cut-off and distinguished between free and aggregate-occluded SOM. In particular, the analyses focused on abundance and composition of two major SOM components, proposed as molecular indicators of fire severity: the non-cellulosic neutral sugars, digested by trifluoroacetic acid (TFA), and the lignin-derived phenolic monomers, released by cupric oxide (CuO) oxidation. The chemical structure of both bulk SOM and SOM fractions were analysed by solid-state C-13 nuclear magnetic resonance spectroscopy. In contrast to the moderately severe fire affecting the Italian site, the extremely severe fire at the Australian site caused substantial loss of SOM from the top mineral soil. Both fires had major effects on SOM composition. In spite of the evident impact they experienced, neither hydrolysable sugars nor lignin phenols resulted to be reliable indicators of fire severity. Moreover, both fires apparently broke up soil aggregates, hence promoting the release of some occluded organic matter. The fire-induced changes of SOM observed have implications for the C cycle, so highlighting the critical role of wildfire occurrence and severity in climate change
A Three Campus Comparison of Bundled Cellular Telephone Features and The Young Consumer
Useable surveys were completed and returned by 507 (174 rural, 156 urban, and 177 Historic Black College and University) consumers at three Midwestern universities. Regression analysis revealed selected demographic variables were predictive of the evaluation of bundled cell phone features; furthermore, stepwise regression models showed among features young consumers reported available on the phones they owned, DIGCAM, EMAIL, WARRANTY, and INSTANTM were significantly predictive (p\u3c.001) of perceptions of importance of digital media bundles on cellular telephones. Moreover, ANOVA tests revealed young consumers’ perceptions of the importance of cellular telephone features were significantly different among rural, urban, and HBCU young consumers, males and females, and among age groups, grade level, and states where cell phones were purchased (p\u3c.05; p\u3c.01; p\u3c.001). Recommendations are offered that could help marketers develop strategic marketing mixes of phone features targeted to young consumers, rural or urban
Organic matter composition and the protist and nematode communities around anecic earthworm burrows
By living in permanent burrows and incorporating organic detritus from the soil surface, anecic earthworms contribute to soil heterogeneity, but their impact is still under-studied in natural field conditions. We investigated the effects of the anecic earthworm Lumbricus centralis on fresh carbon (C) incorporation, soil organic matter composition, protists, and nematodes of a Cambisol under grassland. We used plant material labelled with stable isotope tracers to detect fresh C input around earthworm-occupied burrows or around burrows from which the earthworm had been removed. After 50 days, we sampled soil (0–10 cm depth) in concentric layers around the burrows, distinguishing between drilosphere (0–8 mm) and bulk soil (50–75 mm). L. centralis effectively incorporated fresh C into the drilosphere, and this shifted soil organic matter amount and chemistry: total soil sugar content was increased compared to unoccupied drilosphere and bulk soil, and the contribution of plant-derived sugars to soil organic matter was enhanced. Earthworms also shifted the spatial distribution of soil C towards the drilosphere. The total abundance of protists and nematodes was only slightly higher in earthworm-occupied drilosphere, but strong positive effects were found for some protist clades (e.g. Stenamoeba spp.). Additional data for the co-occurring anecic earthworm species Aporrectodea longa showed that it incorporated fresh C less than L. centralis, suggesting that the two species may have different effects on soil C distribution and organic matter quality
Converting simulated total dry matter to fresh marketable yield for field vegetables at a range of nitrogen supply levels
Simultaneous analysis of economic and environmental performance of horticultural crop production requires qualified assumptions on the effect of management options, and particularly of nitrogen (N) fertilisation, on the net returns of the farm. Dynamic soil-plant-environment simulation models for agro-ecosystems are frequently applied to predict crop yield, generally as dry matter per area, and the environmental impact of production. Economic analysis requires conversion of yields to fresh marketable weight, which is not easy to calculate for vegetables, since different species have different properties and special market requirements. Furthermore, the marketable part of many vegetables is dependent on N availability during growth, which may lead to complete crop failure under sub-optimal N supply in tightly calculated N fertiliser regimes or low-input systems. In this paper we present two methods for converting simulated total dry matter to marketable fresh matter yield for various vegetables and European growth conditions, taking into consideration the effect of N supply: (i) a regression based function for vegetables sold as bulk or bunching ware and (ii) a population approach for piecewise sold row crops. For both methods, to be used in the context of a dynamic simulation model, parameter values were compiled from a literature survey. Implemented in such a model, both algorithms were tested against experimental field data, yielding an Index of Agreement of 0.80 for the regression strategy and 0.90 for the population strategy. Furthermore, the population strategy was capable of reflecting rather well the effect of crop spacing on yield and the effect of N supply on product grading
Auditory hallucinations and migraine of possible brainstem origin
10.1007/s10194-011-0355-zJournal of Headache and Pain125573-57
Research priorities for grassland science: the need of long term integrated experiments networks
Complex osteoclastogenic inductive effects of nicotine over hydroxyapatite
Cigarette smoke is associated to pathological weakening of bone tissue, being considered an important playmaker in conditions such as osteoporosis and periodontal bone loss. In addition, it is also associated with an increased risk of failure in bone regeneration strategies. The present work aimed to characterize the effects of nicotine on human osteoclastogenesis over a hydroxyapatite substrate. Osteoclast precursors were maintained in the absence or presence of the osteoclastogenesis enhancers M-CSF and RANKL, and were further treated with nicotine levels representative of the concentrations observed in the plasma and saliva of smokers. It was observed that nicotine at low concentrations elicit an increase in osteoclast differentiation, but only in the presence of M-CSF and RANKL it was also able to significantly increase the resorbing ability of osteoclasts. A slight downregulation of NFkB pathway and an increase in the production of TNF-α and, particularly PGE2, were involved in the observed effects of nicotine. At high concentrations, nicotine revealed cytotoxic effects, causing a decrease in cell density. In conclusion, nicotine at levels found in the plasma of the smokers, has the ability to act directly on osteoclast precursors, inducing its osteoclastogenic differentiation. The stimulatory behavior appears to be dependent on the stage of osteoclastic differentiation of the precursor cells, which means, in the absence of M-CSF and RANKL, it only favors the initial stages of osteoclast differentiation, while in the presence of the growth factors, a significant increase in their resorbing ability is also achieved.info:eu-repo/semantics/publishedVersio
Lignin biomarkers as tracers of mercury sources in lakes water column
This study presents the role of specific terrigenous organic compounds as important vectors of mercury (Hg) transported from watersheds to lakes of the Canadian boreal forest. In order to differentiate the autochthonous from the allochthonous organic matter (OM), lignin derived biomarker signatures [Lambda, S/V, C/V, P/(V ? S), 3,5-Bd/V and (Ad/Al)v] were used. Since lignin is exclusively produced by terrigenous plants, this approach can give a non equivocal picture of the watershed inputs to the lakes. Moreover, it allows a characterization of the source of OM and its state of degradation. The water column of six lakes from the Canadian Shield was sampled monthly between June and September 2005. Lake total dissolved Hg concentrations and Lambda were positively correlated, meaning that Hg and ligneous inputs are linked (dissolved OM r2 = 0.62, p\0.0001; particulate OM r2 = 0.76, p\0.0001). Ratios of P/(V ? S) and 3,5-Bd/V from both dissolved OM and particulate OM of the water column suggest an inverse relationship between the progressive state of pedogenesis and maturation of the OM in soil before entering the lake, and the Hg concentrations in the water column. No relation was found between Hg levels in the lakes and the watershed flora composition—angiosperm versus gymnosperm or woody versus non-woody compounds. This study has significant implications for watershed management of ecosystems since limiting fresh terrestrial OM inputs should reduce Hg inputs to the aquatic systems. This is particularly the case for largescale land-use impacts, such as deforestation, agriculture and urbanization, associated to large quantities of soil OM being transferred to aquatic systems
Spectroscopic investigations of a semi-synthetic [FeFe] hydrogenase with propane di-selenol as bridging ligand in the binuclear subsite: comparison to the wild type and propane di-thiol variants
[FeFe] Hydrogenases catalyze the reversible conversion of H2 into electrons and protons. Their catalytic site, the H-cluster, contains a generic [4Fe–4S]H cluster coupled to a [2Fe]H subsite [Fe2(ADT)(CO)3(CN)2]2−, ADT = µ(SCH2)2NH. Heterologously expressed [FeFe] hydrogenases (apo-hydrogenase) lack the [2Fe]H unit, but this can be incorporated through artificial maturation with a synthetic precursor [Fe2(ADT)(CO)4(CN)2]2−. Maturation with a [2Fe] complex in which the essential ADT amine moiety has been replaced by CH2 (PDT = propane-dithiolate) results in a low activity enzyme with structural and spectroscopic properties similar to those of the native enzyme, but with simplified redox behavior. Here, we study the effect of sulfur-to-selenium (S-to-Se) substitution in the bridging PDT ligand incorporated in the [FeFe] hydrogenase HydA1 from Chlamydomonas reinhardtii using magnetic resonance (EPR, NMR), FTIR and spectroelectrochemistry. The resulting HydA1-PDSe enzyme shows the same redox behavior as the parent HydA1-PDT. In addition, a state is observed in which extraneous CO is bound to the open coordination site of the [2Fe]H unit. This state was previously observed only in the native enzyme HydA1-ADT and not in HydA1-PDT. The spectroscopic features and redox behavior of HydA1-PDSe, resulting from maturation with [Fe2(PDSe)(CO)4(CN)2]2−, are discussed in terms of spin and charge density shifts and provide interesting insight into the electronic structure of the H-cluster. We also studied the effect of S-to-Se substitution in the [4Fe–4S] subcluster. The reduced form of HydA1 containing only the [4Fe–4Se]H cluster shows a characteristic S = 7/2 spin state which converts back into the S = 1/2 spin state upon maturation with a [2Fe]–PDT/ADT complex
- …