Imaging a Quasar Accretion Disk with Microlensing

We show how analysis of a quasar high-magnification microlensing event may be used to construct a map of the frequency-dependent surface brightness of the quasar accretion disk. The same procedure also allows determination of the disk inclination angle, the black hole mass (modulo the caustic velocity), and possibly the black hole spin. This method depends on the validity of one assumption: that the optical and ultraviolet continuum of the quasar is produced on the surface of an azimuthally symmetric, flat equatorial disk, whose gas follows prograde circular orbits in a Kerr spacetime (and plunges inside the marginally stable orbit). Given this assumption, we advocate using a variant of first-order linear regularization to invert multi-frequency microlensing lightcurves to obtain the disk surface brightness as a function of radius and frequency. The other parameters can be found by minimizing chi-square in a fashion consistent with the regularized solution for the surface brightness. We present simulations for a disk model appropriate to the Einstein Cross quasar, an object uniquely well-suited to this approach. These simulations confirm that the surface brightness can be reconstructed quite well near its peak, and that there are no systematic errors in determining the other model parameters. We also discuss the observational requirements for successful implementation of this technique.Comment: accepted to ApJ for publicatio

GnRH removal in the 7-11 CO-Synch for timed insemination of beef heifers

The use of artificial insemination can improve genetics, shorten the calving season, and increase weaning weights by having more calves born earlier in the breeding season. Reasons that this technology has not been used by many producers may include poor conception rates, time, and cost. Research has been directed at shortening the synchronization time and controlling time of ovulation to eliminate estrous detection using a timed artificial insemination. Use of a progestin, like the Eazi-Breed CIDR or melengestrol acetate (MGA), is desirable because they are effective in synchronizing estrus and they can also induce cyclicity in anestrus or prepubertal females. In addition, gonadotropin releasing hormone (GnRH) is commonly included in a synchronization plan because it induces ovulation and synchronizes follicular development. The most common synchronization protocol for beef heifers consists of feeding MGA for 14 days, a prostaglandin injection 17 to 19 days later followed by five days of heat detection for a total duration of 36 to 38 days. Recent research suggests that acceptable conception rates can be achieved when shortening the time of MGA to seven days. One example is the 7-11 CO-Synch which is comprised of seven days of MGA feeding followed by the CO-Synch protocol starting on day 11 (see Figure 1). We have obtained greater then 60% conception rates using the 7-11 CO-Synch in previous years. The feeding of MGA not only synchronizes the estrous cycle but provides exposure to a progestin that may induce prepubertal heifers to begin cycling. The GnRH injection at day 11 should synchronize the follicular wave which will tighten the timing of ovulation and improve timed insemination conception rates. The disadvantage to this synchronization protocol is that it requires the heifers to go through the chute four times. It is also believed that heifers are less responsive to GnRH than cows. Thus we tested this protocol with or without the day 11 GnRH injection on conception rates of beef heifers

Electronic correlations in vanadium chalcogenides: BaVSe3 versus BaVS3

Albeit structurally and electronically very similar, at low temperature the quasi-one-dimensional vanadium sulfide BaVS3 shows a metal-to-insulator transition via the appearance of a charge-density-wave state, while BaVSe3 apparently remains metallic down to zero temperature. This different behavior upon cooling is studied by means of density functional theory and its combination with the dynamical mean-field theory and the rotationally-invariant slave-boson method. We reveal several subtle differences between these chalcogenides that provide indications for the deviant behavior of BaVSe3 at low temperature. In this regard, a smaller Hubbard U in line with an increased relevance of the Hund's exchange J plays a vital role.Comment: 16 pages, 11 figures, published versio

Approaching finite-temperature phase diagrams of strongly correlated materials: a case study for V2O3

Examining phase stabilities and phase equilibria in strongly correlated materials asks for a next level in the many-body extensions to the local-density approximation (LDA) beyond mainly spectroscopic assessments. Here we put the charge-self-consistent LDA+dynamical mean-field theory (DMFT) methodology based on projected local orbitals for the LDA+DMFT interface and a tailored pseudopotential framework into action in order to address such thermodynamics of realistic strongly correlated systems. Namely a case study for the electronic phase diagram of the well-known prototype Mott-phenomena system V$_2$O$_3$ at higher temperatures is presented. We are able to describe the first-order metal-to-insulator transitions with negative pressure and temperature from the self-consistent computation of the correlated total energy in line with experimental findings.Comment: 12 pages, 15 figures, new data adde

Effect of heifer source on reproductive performance, culling, marketing and profitability for a commercial heifer development program

A commercial heifer development operation purchased 483 weanling Angus × Hereford heifers from 11 sources. Heifers were fed a common silage-based diet through an initial developmental period and retained or culled based on average daily gain, pelvic area, and disposition . The percentage of heifers culled from each source ranged from 18.1% to 94.7% and were either sold directly through a local sale barn or sent to a feedlot with retained ownership . Estrus was synchronized, and heifers were artificially inseminated (AI) for 30 days followed by 15 days of natural mating. First service conception rates for each source ranged from 0% to 92.3%, whereas overall pregnancy rates for the 45-day breeding season ranged from 81.3% to 100%. When expressed as a percentage of the original heifers purchased from each source, overall pregnancy rates ranged from 5.3% to 80%. Heifers that lost their fetuses were sold for a net loss of $213 per head. Heifers sold as first service AI bred, second service AI bred, and naturally mated netted$160, $129, and$89 per head, respectively. With accurate records, stringent culling practices, and evaluation of cost and performance, producers can optimize profit potential of replacement heifers. Early culling and pregnancy diagnosis also will decrease costs while increasing opportunities to minimize the financial risks

Production of Recombinant Adeno-associated Virus Vectors Using Suspension HEK293 Cells and Continuous Harvest of Vector From the Culture Media for GMP FIX and FLT1 Clinical Vector

Adeno-associated virus (AAV) has shown great promise as a gene therapy vector in multiple aspects of preclinical and clinical applications. Many developments including new serotypes as well as self-complementary vectors are now entering the clinic. With these ongoing vector developments, continued effort has been focused on scalable manufacturing processes that can efficiently generate high-titer, highly pure, and potent quantities of rAAV vectors. Utilizing the relatively simple and efficient transfection system of HEK293 cells as a starting point, we have successfully adapted an adherent HEK293 cell line from a qualified clinical master cell bank to grow in animal component-free suspension conditions in shaker flasks and WAVE bioreactors that allows for rapid and scalable rAAV production. Using the triple transfection method, the suspension HEK293 cell line generates greater than 1 × 105 vector genome containing particles (vg)/cell or greater than 1 × 1014 vg/l of cell culture when harvested 48 hours post-transfection. To achieve these yields, a number of variables were optimized such as selection of a compatible serum-free suspension media that supports both growth and transfection, selection of a transfection reagent, transfection conditions and cell density. A universal purification strategy, based on ion exchange chromatography methods, was also developed that results in high-purity vector preps of AAV serotypes 1–6, 8, 9 and various chimeric capsids tested. This user-friendly process can be completed within 1 week, results in high full to empty particle ratios (>90% full particles), provides postpurification yields (>1 × 1013 vg/l) and purity suitable for clinical applications and is universal with respect to all serotypes and chimeric particles. To date, this scalable manufacturing technology has been utilized to manufacture GMP phase 1 clinical AAV vectors for retinal neovascularization (AAV2), Hemophilia B (scAAV8), giant axonal neuropathy (scAAV9), and retinitis pigmentosa (AAV2), which have been administered into patients. In addition, we report a minimum of a fivefold increase in overall vector production by implementing a perfusion method that entails harvesting rAAV from the culture media at numerous time-points post-transfection

Comparison of particle trajectories and collision operators for collisional transport in nonaxisymmetric plasmas

In this work, we examine the validity of several common simplifying assumptions used in numerical neoclassical calculations for nonaxisymmetric plasmas, both by using a new continuum drift-kinetic code and by considering analytic properties of the kinetic equation. First, neoclassical phenomena are computed for the LHD and W7-X stellarators using several versions of the drift-kinetic equation, including the commonly used incompressible-ExB-drift approximation and two other variants, corresponding to different effective particle trajectories. It is found that for electric fields below roughly one third of the resonant value, the different formulations give nearly identical results, demonstrating the incompressible ExB-drift approximation is quite accurate in this regime. However, near the electric field resonance, the models yield substantially different results. We also compare results for various collision operators, including the full linearized Fokker-Planck operator. At low collisionality, the radial transport driven by radial gradients is nearly identical for the different operators, while in other cases it is found to be important that collisions conserve momentum

A novel estrus-synchronization program for anestrous and cycling, suckled, beef cows

We used four herds at three Kansas ranches to evaluate the potential of two new estrus synchronization strategies to increase estrus expression and fertility of 911 crossbred suckled beef cows. The treatments included: 1) 100 μg of GnRH and a 6-mg norgestomet ear implant on day -7 and 25 mg of PG F2" and implant removal on day 0 (GnRH+NORG+PG F2"); 2) 100 μg of GnRH on day - 7 and 25 mg of PGF 2" on day 0 (GnRH+PG F2"); and 3 ) (control) 25- mg injections of PG F2" on days -14 and 0; (2×PGF2" control) . The GnRH+NORG+ PGF 2" and GnRH+PGF treatments increased (P<.01) 2" the overall percentages of cows detected in estrus by 49% and 27% and pregnancy rates by 46% and 37%, respectively, over the control group, without altering conception rate. Both treatments increase d the estrus, conception, and pregnancy rates of noncycling cows, compared to controls