Evaluation of Variation Attributable to Lab and Technician for Measurements of Beef Carcass Traits Made Using Ultrasound

National cattle genetic evaluations assume technician and imaging lab do not contribute to phenotypic variation when measuring carcass traits by ultrasound. The objective of this study was to estimate variance components of ultrasound carcass measurements, specifically variance contributed by ultrasound technician and imaging laboratory. Accounting for technician and imaging lab variation may increase accuracy of genetic predictions for carcass traits. Ultrasound carcass predictions for ribeye area (REA), percent intramuscular fat (IMF), and backfat (BF) were provided by the American Angus Association (AAA; n=281,982 animals), American Hereford Association (AHA; n=49,602), and American Simmental Association (ASA; n=59,576) for a total of 391,160 animals. Data provided by each association included ultrasound carcass measurements, contemporary group, technician ID, imaging lab, and a three-generation pedigree for each animal with ultrasound measurements. Variance contributed by additive genetics, technician, and contemporary group on ultrasound carcass measurements were estimated for each breed separately. Because technician and lab were confounded, the contribution of lab to ultrasound carcass variation could not be separated from technician. Therefore, we assessed whether lab contributed to ultrasound carcass variation by estimating genetic correlations between laboratories for ribeye area, percent intramuscular fat, and backfat. This analysis separated carcass traits by laboratory and treated measurements interpreted by each lab as a separate trait. Technician explained 12-27%, 4-23%, and 4-27% of variance for IMF, BF, and REA respectively across all three breeds. On average, the variance contributed by technician was greater than the variance contributed by additive genetics but less than that explained by contemporary group. Genetic correlations between labs across breeds ranged from 0.79 to 0.95 for IMF, 0.64 to 0.94 for BF and 0.78 to 0.98 for REA. Technician contributed to variance in ultrasound measurements, but high genetic correlations between labs suggest lab plays a lesser role in contribution of variance to ultrasound measurements

Estimating the Effects Weather, Dry Matter Intake, and Body Weight on Daily Water Intake in Weaned Calves

Water is an essential nutrient and is required for growth, metabolism, lactation, and reproduction. However, water requirements for beef cattle have not been studied extensively since the 1950’s. The genetic potential for beef cattle has changed considerably since then. With the change in genetics, beef producers have observed increases in average daily gain and final body weight. The need for updated water requirements for beef cattle has also been exacerbated by global climate variability, drought conditions, and the need for efficient use of water resources. Given the increased productivity of cattle today relative to those of decades ago, increased water requirements are almost certain. Further research must be conducted to determine how those requirements have changed

Humor appreciation of captionless cartoons in obsessive-compulsive disorder

Background: It seems that the core neural regions and cognitive processes implicated in obsessive-compulsive disorder (OCD) pathophysiology may overlap with those involved in humor appreciation. However, to date, there have been no studies that have explored humor appreciation in OCD. The purpose of the present work was to investigate humor appreciation in a group of patients with OCD.Methods: We examined 25 patients with OCD and 25 healthy controls, matched by age, education, and gender. We administered Penn's Humor Appreciation Test (PHAT), a computerized test comprising captionless cartoons by Mordillo. Each set of stimuli consisted of two almost identical drawings, one of which was funny due to the alteration of a detail in the cartoon, whereas the other was not funny. Severity of psychopathology was evaluated with the Yale-Brown Obsessive Compulsive Scale (Y-BOCS).Results: No significant effect for group, gender or group × gender interaction was found on the PHAT scores. In OCD patients, humor appreciation was not significantly associated with age of onset, duration of illness, and obsessions, but correlated significantly with compulsions.Conclusions: Humor appreciation, based on captionless cartoons in OCD, does not seem to be deficient compared to healthy subjects but may be related to illness characteristics. © 2011 Bozikas et al; licensee BioMed Central Ltd

Identification of Genes Associated with Water Restriction Expressed in the Renal Cortex and Hypothalamus in Cattle

The short-term objective of this study is to discover genes associated with water restriction expressed in the renal cortex and hypothalamus in beef calves. The long-term goal is to understand genes and pathways important for thirst response in cattle. This knowledge may lead to discovery of genetic variants associated with water intake. Identification of animals with genetic potential to grow and thrive under drought conditions would be an asset to beef producers and communities which rely on beef production for a large part of their livelihoods. Both selected tissue types are known to be involved in response to hypertonicity (e.g., water restriction or dehydration)

The origins of Amazonian landscapes: Plant cultivation, domestication and the spread of food production in tropical South America

During the last two decades, new archaeological projects which systematically integrate a variety of plant recovery techniques, along with palaeoecology, palaeoclimate, soil science and floristic inventories, have started to transform our understanding of plant exploitation, cultivation and domestication in tropical South America. Archaeobotanical studies are providing a far greater appreciation of the role of plants in the diets of early colonists. Since ~13ka, these diets relied mainly on palm, tree fruits, and underground tubers, along with terrestrial and riverine faunal resources. Recent evidence indicates two areas of precocious plant cultivation and domestication: the sub-Andean montane forest of NW South America and the shrub savannahs and seasonal forests of SW Amazonia. In the latter area, thousands of anthropic keystone structures represented by forest islands show a significant human footprint in Amazonia from the start of the Holocene. While radiocarbon date databases show a decline in population during the middle Holocene, important developments happened during this epoch, including the domestication of cacao, the adoption of maize and the spread of manioc across the basin. The late Holocene witnessed the domestication of rice and the development of agricultural landscapes characterised by raised fields and Amazonian Dark Earths (ADEs). Our multi-proxy analysis of 23 late Holocene ADEs and two lakes from southern Amazonia provides the first direct evidence of field polyculture agriculture including the cultivation of maize, manioc, sweet potato, squash, arrowroot and leren within closed-canopy forest, as well as enrichment with palms, limited clearing for crop cultivation, and low-severity fire management. Collectively, the evidence shows that during the late Holocene Amazonian farmers engaged in intensive agriculture marked by the cultivation of both annual and perennial crops relying on organic amendments requiring soil preparation and maintenance. Our study has broader implications for sustainable Amazonian futures

Comparison of the diagnostic accuracy of three current guidelines for the evaluation of asymptomatic pancreatic cystic neoplasms.

Asymptomatic pancreatic cysts are a common clinical problem but only a minority of these cases progress to cancer. Our aim was to compare the accuracy to detect malignancy of the 2015 American Gastroenterological Association (AGA), the 2012 International Consensus/Fukuoka (Fukuoka guidelines [FG]), and the 2010 American College of Radiology (ACR) guidelines.We conducted a retrospective study at 3 referral centers for all patients who underwent resection for an asymptomatic pancreatic cyst between January 2008 and December 2013. We compared the accuracy of 3 guidelines in predicting high-grade dysplasia (HGD) or cancer in resected cysts. We performed logistic regression analyses to examine the association between cyst features and risk of HGD or cancer.A total of 269 patients met inclusion criteria. A total of 228 (84.8%) had a benign diagnosis or low-grade dysplasia on surgical pathology, and 41 patients (15.2%) had either HGD (n = 14) or invasive cancer (n = 27). Of the 41 patients with HGD or cancer on resection, only 3 patients would have met the AGA guideline\u27s indications for resection based on the preoperative cyst characteristics, whereas 30/41 patients would have met the FG criteria for resection and 22/41 patients met the ACR criteria. The sensitivity, specificity, positive predictive value, negative predictive value of HGD, and/or cancer of the AGA guidelines were 7.3%, 88.2%, 10%, and 84.1%, compared to 73.2%, 45.6%, 19.5%, and 90.4% for the FG and 53.7%, 61%, 19.8%, and 88% for the ACR guidelines. In multivariable analysis, cyst size \u3e3 cm, compared to ≤3 cm, (odds ratio [OR] = 2.08, 95% confidence interval [CI] = 1.11, 4.2) and each year increase in age (OR = 1.07, 95% CI = 1.03, 1.11) were positively associated with risk of HGD or cancer on resection.In patients with asymptomatic branch duct-intraductal papillary mucinous neoplasms or mucinous cystic neoplasms who underwent resection, the prevalence rate of HGD or cancer was 15.2%. Using the 2015 AGA criteria for resection would have missed 92.6% of patients with HGD or cancer. The more inclusive FG and ACR had a higher sensitivity for HGD or cancer but lower specificity. Given the current deficiencies of these guidelines, it will be important to determine the acceptable rate of false-positives in order to prevent a single true-positive

Mediators in the Association Between Affective Temperaments and Suicide Risk Among Psychiatric Inpatients

BACKGROUND: Affective temperaments have been shown to be related to psychiatric disorders and suicidal behaviors. Less is known about the potential contributory role of affective temperaments on suicide risk factors. In the present study, we investigated whether the effect of affective temperaments on suicide risk was mediated by other variables, such as hopelessness, mentalization deficits, dissociation, psychological pain, and depressive symptoms. METHODS: Several assessment instruments, including the Mini International Neuropsychiatric Interview (MINI); the Temperament Evaluation of Memphis, Pisa, and San Diego Autoquestionnaire (TEMPS-A); the Beck Hopelessness Scale (BHS); the Gotland Male Depression Scale (GMDS); the Dissociative Experiences Scale (DES); the Psychological Pain Assessment Scale (PPAS); and the Mentalization Questionnaire (MZQ), were administered to 189 psychiatrically hospitalized patients (103 women, 86 men) in Rome, Italy. RESULTS: In single-mediator models, hopelessness, depressive symptoms, and mentalization, but not psychological pain or dissociation, were significant mediators in the association between prevalent temperament and suicide risk. In a multiple-mediator model, a significant indirect effect was found only for depression. Results demonstrated that patients with negative temperaments reported higher suicide risk, psychological pain, hopelessness, and depression, and less mentalization than patients with no prevalent temperament or hyperthymic temperaments. CONCLUSIONS: Hopelessness, depression, and mentalization are all factors that mediate the relation between affective temperaments and suicide risk. Identifying factors that mediate the effects of affective temperamental makeup on suicide risk should enhance screening and intervention efforts

Influence of Maternal Protein Restriction in Primiparous Heifers During Mid- and/or Late Gestation on Progeny Feedlot Performance and Carcass Characteristics

Maternal nutrient restriction in beef cows impacts developmental processes in the fetus that may influence lifetime performance. This study investigated impacts of metabolizable protein (MP) restriction in primiparous heifers during mid- and/or late-gestation on progeny feedlot performance and carcass characteristics