339 research outputs found
Hydrogen Utilization Potential in Subsurface Sediments
Subsurface microbial communities undertake many terminal electron-accepting processes, often simultaneously. Using a tritium-based assay, we measured the potential hydrogen oxidation catalyzed by hydrogenase enzymes in several subsurface sedimentary environments (Lake Van, Barents Sea, Equatorial Pacific, and Gulf of Mexico) with different predominant electron-acceptors. Hydrogenases constitute a diverse family of enzymes expressed by microorganisms that utilize molecular hydrogen as a metabolic substrate, product, or intermediate. The assay reveals the potential for utilizing molecular hydrogen and allows qualitative detection of microbial activity irrespective of the predominant electron-accepting process. Because the method only requires samples frozen immediately after recovery, the assay can be used for identifying microbial activity in subsurface ecosystems without the need to preserve live material. We measured potential hydrogen oxidation rates in all samples from multiple depths at several sites that collectively span a wide range of environmental conditions and biogeochemical zones. Potential activity normalized to total cell abundance ranges over five orders of magnitude and varies, dependent upon the predominant terminal electron acceptor. Lowest per-cell potential rates characterize the zone of nitrate reduction and highest per-cell potential rates occur in the methanogenic zone. Possible reasons for this relationship to predominant electron acceptor include (i) increasing importance of fermentation in successively deeper biogeochemical zones and (ii) adaptation of H2ases to successively higher concentrations of H2 in successively deeper zones
Interactions between temperature and energy supply drive microbial communities in hydrothermal sediment
Temperature and bioavailable energy control the distribution of life on Earth, and interact with each other due to the dependency of biological energy requirements on temperature. Here we analyze how temperature-energy interactions structure sediment microbial communities in two hydrothermally active areas of Guaymas Basin. Sites from one area experience advective input of thermogenically produced electron donors by seepage from deeper layers, whereas sites from the other area are diffusion-dominated and electron donor-depleted. In both locations, Archaea dominate at temperatures >45 °C and Bacteria at temperatures <10 °C. Yet, at the phylum level and below, there are clear differences. Hot seep sites have high proportions of typical hydrothermal vent and hot spring taxa. By contrast, high-temperature sites without seepage harbor mainly novel taxa belonging to phyla that are widespread in cold subseafloor sediment. Our results suggest that in hydrothermal sediments temperature determines domain-level dominance, whereas temperature-energy interactions structure microbial communities at the phylum-level and below
Can Phlorotannins Purified Extracts Constitute a Novel Pharmacological Alternative for Microbial Infections with Associated Inflammatory Conditions?
Bacterial and fungal infections and the emerging multidrug resistance are driving interest in fighting these microorganisms with natural products, which have generally been considered complementary to pharmacological therapies. Phlorotannins are polyphenols restricted to brown seaweeds, recognized for their biological capacity. This study represents the first research on the antibacterial, antifungal, anti-inflammatory and antioxidant activity of phlorotannins purified extracts, which were obtained from ten dominant brown seaweeds of the occidental Portuguese coast
500,000 Years of Environmental History in Eastern Anatolia: The PALEOVAN Drilling Project
International Continental Scientific Drilling Program
(ICDP) drilled a complete succession of the lacustrine sediment
sequence deposited during the last ~500,000 years in
Lake Van, Eastern Anatolia (Turkey). Based on a detailed
seismic site survey, two sites at a water depth of up to 360 m
were drilled in summer 2010, and cores were retrieved from
sub-lake-floor depths of 140 m (Northern Basin) and 220 m
(Ahlat Ridge). To obtain a complete sedimentary section, the
two sites were multiple-cored in order to investigate the paleoclimate
history of a sensitive semi-arid region between the
Black, Caspian, and Mediterranean seas. Further scientific
goals of the PALEOVAN project are the reconstruction of
earthquake activity, as well as the temporal, spatial, and
compositional evolution of volcanism as reflected in the deposition
of tephra layers. The sediments host organic matter
from different sources and hence composition, which will be
unravelled using biomarkers. Pathways for migration of continental
and mantle-derived noble gases will be analyzed in
pore waters. Preliminary 40Ar/39Ar single crystal dating of
tephra layers and pollen analyses suggest that the Ahlat
Ridge record encompasses more than half a million years of
paleoclimate and volcanic/geodynamic history, providing
the longest continental record in the entire Near East to
date
Exploring subsurface fluid flow and active dewatering along the oceanic plate boundary between Africa and Eurasia (Gloria Fault)
R/V Meteor cruise M162 was conducted as a systematic continuation of ongoing work dedicated to understand if and howfluid flow through crust and sedimentscontinues along transform-type plate boundaries and fracture zones away from mid-ocean ridges and continental margins. Central target was the Gloria Fault in the central Northeast Atlantic. Previous findings along the eastern continuation of the Gloria Fault revealed fault-controlled fluid advection and mud volcanism along strike-slip faults in the Horseshoe Abyssal Plain and the Gulf of Cadiz, where fluid geochemistry revealed the admixture of fluids from deeply buried oceanic crust and oldest sediments on top of it. TheGloria Fault itselfis an old, reactivated, and seismically active oceanic fracture zone. During M162 a systematic survey along the main trace of the Gloria Fault between the Azores Plateau and the Madeira-Tore Rise was carried out, including sub-bottom profiler surveys, heat flow transects, gravity corer sampling, as well as video-guided CTD and multicorer deployments. In accordance to recently recorded seismic activity along the fault, there isevidence for tectonic motion both in sub-bottom profiler records and sediment cores. Heat flow measurements revealed values significantly elevated above the background in many places, predominantly along the main fault trace and other active faults.Ina number of placesfluid geochemistry revealed enhanced diagenetic processes in the sediments, implying the potential relation to upward-directed fluid flow. In summary, cruise M162revealed the first complementary data set on heat flow and fluid geochemistry along an oceanic fault zone, which will further our understanding on themes like the alteration of oceanic lithosphere and crust-ocean element exchange
Design, upgrade and characterization of the silicon photomultiplier front-end for the AMIGA detector at the Pierre Auger Observatory
AMIGA (Auger Muons and Infill for the Ground Array) is an upgrade of the
Pierre Auger Observatory to complement the study of ultra-high-energy cosmic
rays (UHECR) by measuring the muon content of extensive air showers (EAS). It
consists of an array of 61 water Cherenkov detectors on a denser spacing in
combination with underground scintillation detectors used for muon density
measurement. Each detector is composed of three scintillation modules, with 10
m detection area per module, buried at 2.3 m depth, resulting in a total
detection area of 30 m. Silicon photomultiplier sensors (SiPM) measure the
amount of scintillation light generated by charged particles traversing the
modules. In this paper, the design of the front-end electronics to process the
signals of those SiPMs and test results from the laboratory and from the Pierre
Auger Observatory are described. Compared to our previous prototype, the new
electronics shows a higher performance, higher efficiency and lower power
consumption, and it has a new acquisition system with increased dynamic range
that allows measurements closer to the shower core. The new acquisition system
is based on the measurement of the total charge signal that the muonic
component of the cosmic ray shower generates in the detector.Comment: 40 pages, 33 figure
Extraction of the Muon Signals Recorded with the Surface Detector of the Pierre Auger Observatory Using Recurrent Neural Networks
The Pierre Auger Observatory, at present the largest cosmic-ray observatory
ever built, is instrumented with a ground array of 1600 water-Cherenkov
detectors, known as the Surface Detector (SD). The SD samples the secondary
particle content (mostly photons, electrons, positrons and muons) of extensive
air showers initiated by cosmic rays with energies ranging from eV up
to more than eV. Measuring the independent contribution of the muon
component to the total registered signal is crucial to enhance the capability
of the Observatory to estimate the mass of the cosmic rays on an event-by-event
basis. However, with the current design of the SD, it is difficult to
straightforwardly separate the contributions of muons to the SD time traces
from those of photons, electrons and positrons. In this paper, we present a
method aimed at extracting the muon component of the time traces registered
with each individual detector of the SD using Recurrent Neural Networks. We
derive the performances of the method by training the neural network on
simulations, in which the muon and the electromagnetic components of the traces
are known. We conclude this work showing the performance of this method on
experimental data of the Pierre Auger Observatory. We find that our predictions
agree with the parameterizations obtained by the AGASA collaboration to
describe the lateral distributions of the electromagnetic and muonic components
of extensive air showers.Comment: 23 pages, 15 figures. Version accepted for publication in JINS
Design and implementation of the AMIGA embedded system for data acquisition
The Auger Muon Infill Ground Array (AMIGA) is part of the AugerPrime upgrade
of the Pierre Auger Observatory. It consists of particle counters buried 2.3 m
underground next to the water-Cherenkov stations that form the 23.5 km
large infilled array. The reduced distance between detectors in this denser
area allows the lowering of the energy threshold for primary cosmic ray
reconstruction down to about 10 eV. At the depth of 2.3 m the
electromagnetic component of cosmic ray showers is almost entirely absorbed so
that the buried scintillators provide an independent and direct measurement of
the air showers muon content. This work describes the design and implementation
of the AMIGA embedded system, which provides centralized control, data
acquisition and environment monitoring to its detectors. The presented system
was firstly tested in the engineering array phase ended in 2017, and lately
selected as the final design to be installed in all new detectors of the
production phase. The system was proven to be robust and reliable and has
worked in a stable manner since its first deployment.Comment: Accepted for publication at JINST. Published version, 34 pages, 15
figures, 4 table
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