Microarrays as Platform for Multiplex Assays in Biomarker and Drug Discovery

Despite the tremendous advances in the understanding of the molecular mechanisms and the complexity of the diseases is one of the present challenges for the scientific community; then, novel strategies are required to be designed and developed for effective strategies for early diagnosis and treatment. As many cellular alterations are observed at protein level, high-throughput assays are dramatically needed for biomarker discovery. Herein, we describe advantages and limitations of protein microarrays, as proteomics strategy useful for multiplex and high-throughput protein characterization in clinical samples. Finally, a few examples are discussed; mostly of them related to currently disease biomarkers already identified in proximal fluids by protein arrays are discussed

BIM for sustainable management in existing hospitals

The main objective of this communication is to determine the characteristics and requirements of the BIM model, in particular in relation to the BPS energy analysis, to be used as a project tool that supports sustainable management in existing hospital establishments. With this objective, BIM architectural models and environmental simulations of representative buildings in Chile and Spain were carried out to review and propose attributes of sustainable models that allow a better adaptation to growth and flexibility from the early stages of design and restructuring. This study associates low energy consumption strategies for hospital establishments. The study is aimed at the integral management of both the operation of the building and all the services involved in its use and explotation. This will allow to discuss the paradigm changes regarding the training of the architect and the specialists involved in the design, construction and operation of more sustainable projects.Metodología para la valoración de los requisitos de confort, condiciones ambientales y funcionalidad espacial de los hospitales y su entorno. Adecuación de propuestas a nuevos conceptos hospitalarios. Ref.: BIA2016-78893-C3-1-R (Ministerio de Economía y Competitividad

Incidencia de la forma y envolvente en el desempeño térmico de las viviendas en el Centro-Sur de Chile

Este artículo analiza la situación de la vivienda en Chile desde el punto de vista de la eficiencia energética con el fin de proponer mejoras aplicables, en particular en los programas de reconstrucción habitacional post terremoto 2010. El estudio se centra en el centro sur del país, entre la región del Maule y la región de la Araucanía, la zona más afectada por el sismo. Se presentan las condiciones climáticas, aspectos morfológicos y constructivos, para identificar posibles mejoras de eficiencia energética. A partir de este análisis se advierten características del diseño arquitectónico de las viviendas, especialmente de agrupamiento y materialidad de la envolvente que inciden significativamente en su desempeño energético. Se expone una evaluación simple de los costos asociados a los mejoramientos de la envolvente propuestos. Concluyendo que una construcción más eficiente es determinada por un correcto análisis de las condiciones climáticas para la demanda energética correspondiente e identificando formas más adecuadas y calidad de la envolvente. Se constata que modificaciones en el diseño arquitectónico de la vivienda, su emplazamiento y configuración son relevantes en el camino hacia la eficiencia energética.This paper analyses the housing situation in Chile according to energy efficiency, in order to propose improvements, in particular for reconstruction housing programmes post-earthquake of 2010. The study is targeted to the central-south zone of the country, between Maule and Araucanía region; which has been mostly affected by the seismic activity. It reviews climatic conditions, as well as shape and constructive features, in order to identify feasible improvements for energy efficiency. Based on that analysis, it states some housing design properties, in particular related to arrangements and envelope materials with great incidence on the environmental performance. A simple assessment of the costs associated to these improvements is made. The work concludes that a more efficient construction is determined by the correct analysis of climatic conditions for the energy requirements, to identify proper shapes and a quality of envelope. It demonstrates that modifications of housing design, location and construction can be significant in the way to energy efficiency.Diseño Integrado para la Reconstrucción de Viviendas Energeticamente Eficientes (Conicyt-MEL 8110003

Incidence of form and envelope in thermal behavior of dwellings in the centre-south of Chile

Este artículo analiza la situación de la vivienda en Chile desde el punto de vista de la eficiencia energética con el fin de proponer mejoras aplicables, en particular en los programas de reconstrucción habitacional post terremoto 2010. El estudio se centra en el centro sur del país, entre la región del Maule y la región de la Araucanía, la zona más afectada por el sismo. Se presentan las condiciones climáticas, aspectos morfológicos y constructivos, para identificar posibles mejoras de eficiencia energética. A partir de este análisis se advierten características del diseño arquitectónico de las viviendas, especialmente de agrupamiento y materialidad de la envolvente que inciden significativamente en su desempeño energético. Se expone una evaluación simple de los costos asociados a los mejoramientos de la envolvente propuestos. Concluyendo que una construcción más eficiente es determinada por un correcto análisis de las condiciones climáticas para la demanda energética correspondiente e identificando formas más adecuadas y calidad de la envolvente. Se constata que modificaciones en el diseño arquitectónico de la vivienda, su emplazamiento y configuración son relevantes en el camino hacia la eficiencia energética.This paper analyses the housing situation in Chile according to energy efficiency, in order to propose improvements, in particular for reconstruction housing programmes post-earthquake of 2010. The study is targeted to the central-south zone of the country, between Maule and Araucanía region; which has been mostly affected by the seismic activity. It reviews climatic conditions, as well as shape and constructive features, in order to identify feasible improvements for energy efficiency. Based on that analysis, it states some housing design properties, in particular related to arrangements and envelope materials with great incidence on the environmental performance. A simple assessment of the costs associated to these improvements is made. The work concludes that a more efficient construction is determined by the correct analysis of climatic conditions for the energy requirements, to identify proper shapes and a quality of envelope. It demonstrates that modifications of housing design, location and construction can be significant in the way to energy efficiency

Estratificación del riesgo en el dolor torácico en urgencias. Revisión sistemática

Abstract Title: Importance of risk stratification in chest pain in the emergency department. Systematic review. Chest pain is a frequent reason for consultation in Emergency Department. Is characterized by great heterogeneity regarding chest presentation, final diagnosis and the risk for the patient. An adequate risk stratification is crucial for a properly and complete initial from Emergency Department. Evaluation. For this reason is required the knowledge and use of Risk Stratificacion Scalespara lo que se requiere el conocimiento y uso de las Escalas de Estratificación del Riesgo disponibles. Objective: To compare different risk stratification tools in patients with chest pain in the Emergency Department, related to the discrimination of subjects with increased risk of major adverse cardiac events. Methodology: A Systematic Review in databases CINAHL, PUBMED, CUIDEN, EMBASE, LILACS and SCIELO. Were included studies using a risk stratification tools to predict major adverse cardiac events. Results: 12 studies were included. These studies identified 7 risk stratification tools. The TIMI Risk Score was the most used. Of them, TIMI, GRACE, HEART Score and TIMI Modified received the highest discrimination capability (c>0.70), with the higher score for HEART Score. Conclusions: TIMI, GRACE and HEART Score are the largely risk stratification tools investigated, HEART Score showed the highest predictive capacity to major adverse cardiac events. Others risk stratification tools have been identified, however have not been conducted a rigorous validation, this is defined as a future research line. Key words Chest Pain; Acute Coronary Syndrome; Prognosis; Emergency Department; Risk Assessment

Dynamic Intracellular Metabolic Cell Signaling Profiles During Ag-Dependent B-Cell Differentiation

© 2021 Díez, Pérez-Andrés, Bøgsted, Azkargorta, García-Valiente, Dégano, Blanco, Mateos-Gomez, Bárcena, Santa Cruz, Góngora, Elortza, Landeira-Viñuela, Juanes-Velasco, Segura, Manzano-Román, Almeida, Dybkaer, Orfao and Fuentes.Human B-cell differentiation has been extensively investigated on genomic and transcriptomic grounds; however, no studies have accomplished so far detailed analysis of antigen-dependent maturation-associated human B-cell populations from a proteomic perspective. Here, we investigate for the first time the quantitative proteomic profiles of B-cells undergoing antigen-dependent maturation using a label-free LC-MS/MS approach applied on 5 purified B-cell subpopulations (naive, centroblasts, centrocytes, memory and plasma B-cells) from human tonsils (data are available via ProteomeXchange with identifier PXD006191). Our results revealed that the actual differences among these B-cell subpopulations are a combination of expression of a few maturation stage-specific proteins within each B-cell subset and maturation-associated changes in relative protein expression levels, which are related with metabolic regulation. The considerable overlap of the proteome of the 5 studied B-cell subsets strengthens the key role of the regulation of the stoichiometry of molecules associated with metabolic regulation and programming, among other signaling cascades (such as antigen recognition and presentation and cell survival) crucial for the transition between each B-cell maturation stage.We gratefully acknowledge financial support from the Spanish Health Institute Carlos III (ISCIII) for the grants: FIS PI14/01538, FIS PI17/01930 and CB16/12/00400. We also acknowledge Fondos FEDER (EU) and Junta Castilla-León (COVID19 grant COV20EDU/00187). Fundación Solórzano FS/38-2017.The Proteomics Unit belongs to ProteoRed, PRB3-ISCIII, supported by grant PT17/0019/0023, of the PE I + D + I 2017-2020, funded by ISCIII and FEDER. AL-V is supported by VIII Centenario-USAL PhD Program. PJ-V is supported by JCYL PhD Program and scholarship JCYL-EDU/601/2020. PD and EB are supported by a JCYL-EDU/346/2013 Ph.D. scholarship

Incidencia de la forma y envolvente en el desempeño térmico de las viviendas del centro-sur de Chile

Este artículo analiza la situación de la vivienda en Chile desde el punto de vista de la eficiencia energética con el fin de proponer mejoras aplicables, en particular en los programas de reconstrucción habitacional post terremoto 2010. El estudio se centra en el centro sur del país, entre la región del Maule y la región de la Araucanía, la zona más afectada por el sismo. Se presentan las condiciones climáticas, aspectos morfológicos y constructivos, para identificar posibles mejoras de eficiencia energética. A partir de este análisis se advierten características del diseño arquitectónico de las viviendas, especialmente de agrupamiento y materialidad de la envolvente que inciden significativamente en su desempeño energético. Se expone una evaluación simple de los costos asociados a los mejoramientos de la envolvente propuestos. Concluyendo que una construcción más eficiente es determinada por un correcto análisis de las condiciones climáticas para la demanda energética correspondiente e identificando formas más adecuadas y calidad de la envolvente. Se constata que modificaciones en el diseño arquitectónico de la vivienda, su emplazamiento y configuración son relevantes en el camino hacia la eficiencia energética