Kapsaisin Katkılı Tris Sulandırıcı Kullanılarak Koç Spermasının Dondurularak Saklanması

In this study, it was designed to reveal the effects of capsaicin on oxidative stress and freezability of ram semen. Ejaculates were taken from Sönmez rams and divided into five specimens and diluted with extender at different rates (4 mM, 2 mM, 1 mM, 500 μM) with and without capsaicin (control; C). Semen samples were thawed with a 37°C water bath for 30 seconds for post-thawed analysis. At the end of the study, sperm motility and kinetic parameters, plasma membrane acrosome integrity (PMAI), mitochondrial membrane potential (MMP), DNA damage, oxidant and antioxidant parameters were analyzed. A decrease was observed in the groups containing capsaicin compared to the C in terms of progressive, total motility and kinetic parameters (p<0.05). Besides, positive results were not obtained DNA integrity, PMAI and MMP (p<0.05). In conclusion; it was determined that capsaicin added to Tris extender did not have a positive effect on oxidative stress and freezing of ram semen.Bu çalışmada, kapsaisinin oksidatif stres ve koç spermasının dondurulabilirliği üzerine etkilerinin ortaya konması tasarlandı. Ejakülatlar Sönmez ırkı koçlardan alınarak beş eşit kısma ayrıldı ve farklı oranlarda (4 mM, 2 mM, 1 mM, 500 μM) kapsaisin içeren ve içermeyen (kontrol) sulandırıcı ile sulandırıldı. Sperma örnekleri çözüm sonu spermatolojik analizler için 37°C sıcaklıklta 30 saniye süre ile çözdürüldü. Çalışma sonunda spermatozoa hareketliliği ve kinetik parametreleri, plazma membran akrozom bütünlüğü (PMAI), mitokondrial membran potansiyeli (MMP), DNA hasarı, oksidan ve antioksidan parametreler analiz edildi. Progresif, total motilite ve kinetik parametreler bakımından kontrol grubuna göre kapsaisin içeren guruplarda azalma görüldü (p<0.05). Ayrıca DNA bütünlüğü, PMAI ve MMP'de olumlu sonuçlar elde edilmedi (p<0.05). Sonuç olarak; Tris sulandırıcısına ilave edilen kapsaisinin, oksidatif stres ve koç spermasının dondurulması üzerine olumlu bir etki göstermediği belirlendi

Investigation of changes in spermatozoon characteristics, chromatin structure, and antioxidant/oxidant parameters after freeze-thawing of hesperidin (Vitamin P) doses added to ram semen

We conducted this study to determine the potential cryopreservative effects of different hesperidin (vitamin P; H) doses on ram semen after freeze-thawing. Semen samples were obtained from Sonmez rams using an artificial vagina. The samples were divided into six groups: control, 10, 50, 100, 250, and 500 mu g/mL H (C, H10, H50, H100, H250, and H500, respectively). At the end of the study, sperm motility and kinetic parameters, acrosome integrity (AI), mitochondrial membrane potential (MMP), viability, lipid peroxidation levels (LPL), chromatin damage, oxidant parameters, and antioxidant parameters were assayed. None of the doses of H added to the semen extender showed any enhancing effects on progressive motility compared to C (p > 0.05). In fact, H500 had negative effects (p < 0.05). Moreover, AI was the highest at the H10 dose, while LPL values were the lowest at the same dose (p < 0.05). The doses of H10 and H50 added to the Tris extender medium showed positive effects on sperm cell chromatin damage. Consequently, we can say that H doses used in this study are not effective on semen progressive motility, but the H10 dose is effective on AI and chromatin damage by reducing LPL

Lausanne conference Turkish-Greek population exchange and the conditions of non-exchanged

YÖK Tez ID: 440094Türk-Yunan "nüfus mübadelesi", Lozan Konferansı'nda Türkiye ve Yunanistan arasındaki öncelikli sorunlardan birisi olmuş ve 30 Ocak 1923 tarihinde, Türkiye ve Yunanistan arasında imzalanan 'Yunan ve Türk Halklarının Mübadelesine İlişkin Sözleşme ve Protokol'ün imzalanmasıyla çözüme kavuşturulmuştur. Mübadele işlemi süresince (1923- 1925) 1.200.000 Rum'a karşılık 400.000 Müslüman Türk zorunlu göçe tâbi tutulmuştur. Türk ve Yunan makamlarının karşılıklı isteği doğrultusunda ve Konferansa katılan diğer ülkelerin desteğiyle çok kısa bir zamanda imzalanan sözleşmenin uygulanması, ne yazık ki, o kadar kolay olmamıştır. Bu sözleşmenin 11. maddesi uyarınca yapılacak mübadelenin uygulanmasında karşılaşılabilecek sorunları çözümlemesi amacıyla da Muhtelit Mübadele Komisyonu kurulmuştur. Komisyon çalışmalarına Ekim 1923'de başlamış ve önemli bir sorunla karşılaşmadan 1925 yılının Nisan ayına kadar bir kısım Rum ve Türk halklarının mübadelesini sağlamıştır. Ancak sözleşme metnindeki bazı eksiklikler ve belirsiz ifadeler iki tarafın da gündemini uzun süre meşgul etmiştir. Sözleşmenin ikinci maddesinde yer alan etablis (yerleşmiş) kelimesinin Türk ve Yunan komisyon üyelerince farklı yorumlanması iki ülke arasındaki ilişkileri kopma noktasına getiren "etablis sorunu" da denilen anlaşmazlığa yol açmıştır. 24 Temmuz 1923 tarihli Lozan Barış Antlaşması'nın 37-44. Maddeleri mübadele dışında bırakılan Batı Trakya Müslüman Türk toplumunu ve İstanbul Ortodoks Rum toplumunu etablis kabul ederek, azınlık statüsü vermiştirTurkish-Greek "Population Exchange" has been one of the main issues between Turkey and Greece in the Lausanne Conference and has been resolved with the signing of the contract and the protocol on the exchange of the Greek and Turkish population respectively signed between Turkey and Greece in January 30, 1923. During the exchange process, 400.000 Muslim Turks were subjected to forced migration against 1.200.000 Greeks. Unfortunately, implementation of the agreement that signed in a very short time with the support of other countries participating in the conference, was not very easy. According to the 11th substance of the agreement, "A mixed exchange commission" was established in order to solve problems which will be encountered during the implementation of exchange. The commission began its work in October 1923 and the exchange of some Greek and Turkish people have been provided without any important problem until April 1925. But some shortcoming and uncertain expressions in the text of the convention occupied both sides for a long time. Turkish and Greek Commission members have commented the word Etablis in different ways. This has caused "the question of etablis" and the relationship of the two countries has come to the breaking point. 37th and 44th Substance of Lausanne Peace Treaty of July 24, 1923 regarded them as etablis and gave minority status to Western Thrace Turkish Community and Istanbul Greek Orthodox Communit

Comparison of double inversion recovery and FLAIR sequences in diagnosing multiple sclerosis infratentorial plaques

Fluid–Attenuated Inversion Recovery (FLAIR) is the most commonly used sequence in routine practice for MS. Recent studies employ alternative sequences such as Double Inversion Recovery (DIR) in addition to conventional MRI techniques. In this study we compared these two sequences for their sensitivity in detecting infratentorial lesions in MS. A total of 24 patients with 3D DIR and 3D FLAIR sequences were included in this study. Plaques were classified according to their localization such as brainstem and cerebellar plaques. The relationship between number of infratentorial plaques and age, gender, MS type, disease duration, average annual number of attacks, cerebellar atrophy existence and EDSS score was also analyzed. DIR sequence detected higher number of lesions compared to FLAIR sequence in brainstem (59 vs 45 plaque in 24 cases) and cerebellum (50 vs 25 plaque in 24 cases). In detecting cerebellar lesions, superiority of DIR sequence compared to FLAIR sequence was statistically significant (p=0.049). Patients with longer disease duration had less cerebellar lesion load in FLAIR sequence at a statistically significant rate. Patients with longer disease duration and higher number of attacks had higher EDSS scores. There was no relationship between brainstem plaques number of cerebellum plaques and EDSS score. Comparing groups with and without cerebellar atrophy, cerebellar atrophy was also more frequent in patients with higher level of cerebellar lesion load in DIR sequence (p=0.028). Our findings suggest that DIR sequence is superior to FLAIR sequence in detecting cerebellar lesions. Cerebellar lesion load detected in DIR sequence was correlated to cerebellar atrophy. For this reason, especially during early period of disease, DIR sequence may be more useful than FLAIR sequence in distinguishing definite MS from clinical isolated syndromes and also for determining atrophy risk

Efficiency of double inversion recovery (DIR) sequence in the evaluation of supratentorial cortical lesions in multiple sclerosis

WOS: 000429001000004The aim of this study is to investigate if the three-dimensional (3D) double inversion recovery (DIR) sequence is superior compared to the 3D fluid-attenuated inversion-recovery (FLAIR) sequence in detecting intracortical, mixed, juxtacortical (JX), and deep grey matter (GM) lesions in multiple sclerosis (MS) patients. The correlation between intracortical lesion load and disability status was also investigated. Magnetic resonance imaging examinations of 24 patients (9 males, 15 females; mean age 34.4 +/- 12.0 (16-69) were retrospectively evaluated from our database. Lesions were counted and classified according to anatomic regions as intracortical, mixed, JX, and deep GM on the 3D DIR and FLAIR sequences. The incidence of lesions on the two sequences were identified and compared. The relationship between the number of lesions and type of MS, patient age, gender, duration of the disease, disability, the mean number of attacks per year and Expanded Disability Status Scale (EDSS) score was also investigated. More lesions were detected by the DIR sequence compared to the FLAIR sequence in all regions except for thalamus (11/12). The lesion detection superiority of DIR was statistically significant for intracortical lesions (p<0.001) and GM lesions (intracortical + mixed) (p<0.001). Lesion load of the JX area in the DIR sequence decreased as the disease duration (r=-0.444; p=0.030) and age (r=-0.473; p=0.020) increased. JX lesion load in the DIR sequence decreased as the number of attacks increased (r=-0.602; p=0.002). More mixed lesion load on the DIR sequence were found in cerebral atrophy group than in no cerebral atrophy group (p=0.026). EDSS score increased as the disease duration and number of attacks increased (p=0.003, p<0.001). There was no correlation between lesion location and EDSS score. The DIR sequence is superior to the FLAIR sequence in the detection of intracortical and GM lesions. Also, the mixed lesion load on the DIR sequence is correlated with cerebral atrophy