Determination of lactadherin concentration in dairy by-products by ELISA: Effect of heat treatment and hydrolysis

Lactadherin is a peripheral glycoprotein of the milk fat globule membrane with several attributed biological activities. In this study, we developed an indirect competitive ELISA to determine lactadherin concentration by using a rabbit polyclonal antiserum. The ELISA was applied to quantify lactadherin in several dairy by-products. Of the products tested, raw and commercial buttermilk had the highest concentrations of lactadherin (6.79 and 5.27 mg/g of product, respectively), followed by commercial butter serum (4.86 mg/g), commercial skim milk (4.84 mg/g), and raw whey (1.20 mg/g). The concentration of immunoreactive lactadherin was also determined in dairy by- products after they were subjected to different technological treatments. Thus, raw products were heat treated at combinations of temperature and time typically used in the dairy industry, and commercial products were hydrolyzed using 3 proteolytic enzyme preparations. Heat treatments of whey and buttermilk resulted in a smaller decrease in lactadherin concentration than did hydrolysis as determined by ELISA and electrophoresis. At high temperatures for long durations, the loss of lactadherin was higher in whey than in buttermilk, with the maximal reduction of around 48% found after treating whey at 72 degrees C for 60 min. Hydrolysis of commercial products with proteolytic enzymes resulted in a marked decrease of immunoreactivity within the first 5 min of treatment, which thereafter was constant throughout 4 h of hydrolysis. These results demonstrate that dairy by- products from milk fat processing are good natural sources of lactadherin, although technological processes have to be considered, because they have different effects on lactadherin content

Simulations of Alfvénic modes in TJ-II Stellarator

Alfvénic modes are one of the subclass of instabilities prevalent in burning plasmas due to interaction of energetic particles with background plasma. In this paper we investigate the properties of these modes with 3D simulations using modeling tools STELLGAP [1] and AE3D [2] of Neutral Beam Injection (NBI) heated H-plasmas in TJ-II low-magnetic-shear flexible heliac (B0 = 0.95 T, = 1.5 m, = 0.22 m). These simulations focus on modelling the experimental observations [3] for prominent modes in TJ-II plasmas. Our simulations show consistency in frequency and radial location with the measured Alfvén Eigenmodes [3]. These simulations are performed for chirping and steady modes in TJ-II discharge # 29839 at t = 1150 and 1160ms respectively

Observation of confined current ribbon in JET plasmas

we report the identification of a localised current structure inside the JET plasma. It is a field aligned closed helical ribbon, carrying current in the same direction as the background current profile (co-current), rotating toroidally with the ion velocity (co-rotating). It appears to be located at a flat spot in the plasma pressure profile, at the top of the pedestal. The structure appears spontaneously in low density, high rotation plasmas, and can last up to 1.4 s, a time comparable to a local resistive time. It considerably delays the appearance of the first ELM.Comment: 10 pages, 6 figure

Electron Bernstein waves emission in the TJ-II Stellarator

Taking advantage of the electron Bernstein waves heating (EBWH) system of the TJ-II stellarator, an electron Bernstein emission (EBE) diagnostic was installed. Its purpose is to investigate the B-X-O radiation properties in the zone where optimum theoretical EBW coupling is predicted. An internal movable mirror shared by both systems allows us to collect the EBE radiation along the same line of sight that is used for EBW heating. The theoretical EBE has been calculated for different orientations of the internal mirror using the TRUBA code as ray tracer. A comparison with experimental data obtained in NBI discharges is carried out. The results provide a valuable information regarding the experimental O-X mode conversion window expected in the EBW heating experiments. Furthermore, the characterization of the radiation polarization shows evidence of the underlying B-X-O conversion process.Comment: 21 pages, 14 figure

Mental disorders of known aetiology and precision medicine in psychiatry: a promising but neglected alliance [Editorial]

Personalized or precision medicine is predicated on the assumption that the average response to treatment is not necessarily representative of the response of each individual. A commitment to personalized medicine demands an effort to bring evidence-based medicine and personalized medicine closer together. The use of relatively homogeneous groups, defined using a priori criteria, may constitute a promising initial step for developing more accurate risk-prediction models with which to advance the development of personalized evidence-based medicine approaches to heterogeneous syndromes such as schizophrenia. However, this can lead to a paradoxical situation in the field of psychiatry. Since there has been a tendency to loosely define psychiatric disorders as ones without a known aetiology, the discovery of an aetiology for psychiatric syndromes (e.g. 22q11.2 deletion syndrome in some cases of schizophrenia), while offering a path toward more precise treatments, may also lead to their reclassification away from psychiatry. We contend that psychiatric disorders with a known aetiology should not be removed from the field of psychiatry. This knowledge should be used instead to guide treatment, inasmuch as psychotherapies, pharmacotherapies and other treatments can all be valid approaches to mental disorders. The translation of the personalized clinical approach inherent to psychiatry into evidence-based precision medicine can lead to the development of novel treatment options for mental disorders and improve outcomes

Cognitive clusters in first-episode psychosis

Impairments in a broad range of cognitive domains have been consistently reported in some individuals with first-episode psychosis (FEP). Cognitive deficits can be observed during the prodromal stage. However, the course of cognitive deficits is still unclear. The aim of this study was to identify cognitive subgroups over time and to compare their sociodemographic, clinical and functional profiles. A total of 114 patients with Schizophrenia Spectrum Disorders were included in the present study. We assessed subjects through psychiatric scales and eight neuropsychological tests at baseline and at two-year follow-up visit. We performed the Partition Around Medoids algorithm with all cognitive variables. Furthermore, we performed a logistic regression to identify the predictors related to the different cognitive clusters at follow-up. Two distinct subgroups were found: the first cluster characterized by cognitive impairment and a second cluster had relatively intact cognition in comparison with norms. Up to 54.7% of patients with cognitive deficits at baseline tended to improve during the first two years of treatment. Patients with intact cognition at follow-up had a higher socioeconomic status, later age of onset, lower negative symptoms and a higher cognitive reserve (CR) at baseline. CR and age of onset were the baseline variables that predicted cognitive impairment. This research allows us to obtain a better understanding of the heterogeneous profile of psychotic disorders. Identifying the characteristics of patients who will present a cognitive impairment could improve early detection and intervention. These results suggest that enhancing CR could contribute to improving the course of the illness. © 2021 Elsevier B.V

Inorganic and organic geochemical fingerprinting of sediment sources and ocean circulation on a complex continental margin (São Paulo Bight, Brazil)

In this study, we use inorganic (metal) and organic (bulk and molecular) markers in sediment samples of the south-eastern Brazilian margin to investigate the response of geochemical fingerprints to the complex hydrodynamic processes present in the area. Results indicate the potential of export of terrigenous siliciclastic and organic constituents to the upper slope, even in an area with limited fluvial supply. Metal contents and especially the ln(Ti = Al) and ln(Fe = K) ratios make it possible to recognise the extension of shelf sediments toward the upper slope. Potassium, here expressedas ln(K = Sc) and ln(K = Al) ratios used as proxies of illite–kaolinite variations, proved to be an important parameter, especially because it allowed us to decipher the imprint of the northward flow of the Intermediate Western Boundary Current (IWBC) in comparison to the southward flows of the Brazil Current (BC) and Deep Western Boundary Current (DWBC). Using organic matter analyses, we were able to evaluate the extent of terrestrial contributions to the outer shelf and slope, even without the presence of significant fluvial input. In addition, molecular markers signify a slight increase in the input of C4-derived plants to the slope sediments, transported from distant areas by the main alongshore boundary currents, indicating that the terrestrial fraction of the organic matter deposited on the slope has a distinct origin when compared to shelf sediments