Numerical Simulations of Dynamos Generated in Spherical Couette Flows

We numerically investigate the efficiency of a spherical Couette flow at generating a self-sustained magnetic field. No dynamo action occurs for axisymmetric flow while we always found a dynamo when non-axisymmetric hydrodynamical instabilities are excited. Without rotation of the outer sphere, typical critical magnetic Reynolds numbers $Rm_c$ are of the order of a few thousands. They increase as the mechanical forcing imposed by the inner core on the flow increases (Reynolds number $Re$). Namely, no dynamo is found if the magnetic Prandtl number $Pm=Rm/Re$ is less than a critical value $Pm_c\sim 1$. Oscillating quadrupolar dynamos are present in the vicinity of the dynamo onset. Saturated magnetic fields obtained in supercritical regimes (either $Re>2 Re_c$ or $Pm>2Pm_c$) correspond to the equipartition between magnetic and kinetic energies. A global rotation of the system (Ekman numbers $E=10^{-3}, 10^{-4}$) yields to a slight decrease (factor 2) of the critical magnetic Prandtl number, but we find a peculiar regime where dynamo action may be obtained for relatively low magnetic Reynolds numbers ($Rm_c\sim 300$). In this dynamical regime (Rossby number $Ro\sim -1$, spheres in opposite direction) at a moderate Ekman number ($E=10^{-3}$), a enhanced shear layer around the inner core might explain the decrease of the dynamo threshold. For lower $E$ ($E=10^{-4}$) this internal shear layer becomes unstable, leading to small scales fluctuations, and the favorable dynamo regime is lost. We also model the effect of ferromagnetic boundary conditions. Their presence have only a small impact on the dynamo onset but clearly enhance the saturated magnetic field in the ferromagnetic parts. Implications for experimental studies are discussed

On the magnetic fields generated by experimental dynamos

We review the results obtained by three successful fluid dynamo experiments and discuss what has been learnt from them about the effect of turbulence on the dynamo threshold and saturation. We then discuss several questions that are still open and propose experiments that could be performed to answer some of them.Comment: 40 pages, 13 figure

The Euchromatic and Heterochromatic Landscapes Are Shaped by Antagonizing Effects of Transcription on H2A.Z Deposition

A role for variant histone H2A.Z in gene expression is now well established but little is known about the mechanisms by which it operates. Using a combination of ChIP–chip, knockdown and expression profiling experiments, we show that upon gene induction, human H2A.Z associates with gene promoters and helps in recruiting the transcriptional machinery. Surprisingly, we also found that H2A.Z is randomly incorporated in the genome at low levels and that active transcription antagonizes this incorporation in transcribed regions. After cessation of transcription, random H2A.Z quickly reappears on genes, demonstrating that this incorporation utilizes an active mechanism. Within facultative heterochromatin, we observe a hyper accumulation of the variant histone, which might be due to the lack of transcription in these regions. These results show how chromatin structure and transcription can antagonize each other, therefore shaping chromatin and controlling gene expression

Lobe Specific Ca2+-Calmodulin Nano-Domain in Neuronal Spines: A Single Molecule Level Analysis

Calmodulin (CaM) is a ubiquitous Ca2+ buffer and second messenger that affects cellular function as diverse as cardiac excitability, synaptic plasticity, and gene transcription. In CA1 pyramidal neurons, CaM regulates two opposing Ca2+-dependent processes that underlie memory formation: long-term potentiation (LTP) and long-term depression (LTD). Induction of LTP and LTD require activation of Ca2+-CaM-dependent enzymes: Ca2+/CaM-dependent kinase II (CaMKII) and calcineurin, respectively. Yet, it remains unclear as to how Ca2+ and CaM produce these two opposing effects, LTP and LTD. CaM binds 4 Ca2+ ions: two in its N-terminal lobe and two in its C-terminal lobe. Experimental studies have shown that the N- and C-terminal lobes of CaM have different binding kinetics toward Ca2+ and its downstream targets. This may suggest that each lobe of CaM differentially responds to Ca2+ signal patterns. Here, we use a novel event-driven particle-based Monte Carlo simulation and statistical point pattern analysis to explore the spatial and temporal dynamics of lobe-specific Ca2+-CaM interaction at the single molecule level. We show that the N-lobe of CaM, but not the C-lobe, exhibits a nano-scale domain of activation that is highly sensitive to the location of Ca2+ channels, and to the microscopic injection rate of Ca2+ ions. We also demonstrate that Ca2+ saturation takes place via two different pathways depending on the Ca2+ injection rate, one dominated by the N-terminal lobe, and the other one by the C-terminal lobe. Taken together, these results suggest that the two lobes of CaM function as distinct Ca2+ sensors that can differentially transduce Ca2+ influx to downstream targets. We discuss a possible role of the N-terminal lobe-specific Ca2+-CaM nano-domain in CaMKII activation required for the induction of synaptic plasticity

The HIV Envelope but Not VSV Glycoprotein Is Capable of Mediating HIV Latent Infection of Resting CD4 T Cells

HIV fusion and entry into CD4 T cells are mediated by two receptors, CD4 and CXCR4. This receptor requirement can be abrogated by pseudotyping the virion with the vesicular stomatitis virus glycoprotein (VSV-G) that mediates viral entry through endocytosis. The VSV-G-pseudotyped HIV is highly infectious for transformed cells, although the virus circumvents the viral receptors and the actin cortex. In HIV infection, gp120 binding to the receptors also transduces signals. Recently, we demonstrated a unique requirement for CXCR4 signaling in HIV latent infection of blood resting CD4 T cells. Thus, we performed parallel studies in which the VSV-G-pseudotyped HIV was used to infect both transformed and resting T cells in the absence of coreceptor signaling. Our results indicate that in transformed T cells, the VSV-G-pseudotyping results in lower viral DNA synthesis but a higher rate of nuclear migration. However, in resting CD4 T cells, only the HIV envelope-mediated entry, but not the VSV-G-mediated endocytosis, can lead to viral DNA synthesis and nuclear migration. The viral particles entering through the endocytotic pathway were destroyed within 1–2 days. These results indicate that the VSV-G-mediated endocytotic pathway, although active in transformed cells, is defective and is not a pathway that can establish HIV latent infection of primary resting T cells. Our results highlight the importance of the genuine HIV envelope and its signaling capacity in the latent infection of blood resting T cells. These results also call for caution on the endocytotic entry model of HIV-1, and on data interpretation where the VSV-G-pseudotyped HIV was used for identifying HIV restriction factors in resting T cells

Simulations of beam ion transport during tearing modes in the DIII-D tokamak

Large coherent MHD modes are observed to reduce the neutral beam current drive efficiency and 2.5 MeV neutron emission in DIII-D by as much as ∼65%. These modes result in large (width w ≲ 20 cm for minor radius a ≈ 60 cm), stationary, single helicity magnetic islands, which might cause anomalous deuterium beam ion losses through orbit stochasticity. An analytic estimate predicts that co-going, passing deuterons with E ≳ 40 keV become stochastic at island widths comparable to those in the experiment. A Hamiltonian guiding centre code is used to follow energetic particle trajectories with the tearing mode modelled as a radially extended, single helicity perturbation. In the simulations, the lost neutral beam current drive and neutron emission are 35% and 40%, respectively, which is consistent with the measured reductions of 40 ± 14% and 40 ± 10%. Several features of the lost particle distribution indicate that orbit stochasticity is the loss mechanism in the simulations and strongly suggest that the same mechanism is responsible for the losses observed in the experiment

Reduction in neutral beam driven current in a tokamak by tearing modes

Profiles of noninductive current driven by neutral beam injection into a tokamak have been measured and compared with theory. The driven current can be less than the theoretical prediction (by up to 80%) in the presence of islands driven by tearing modes. © 1997 The American Physical Society