Data in support of comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance

AbstractHere, we present the data from a comparative physiology and proteomics approach used to analyze the response of two wheat genotypes (SERI M 82 (SE) and SW89.5193/kAu2 (SW)) with contrasting responses to drought stress. Proteomic analysis resulted in identification of 49 unique proteins with significant change in abundance (2-fold) under water shortage in roots and leaves. Gene ontology analysis of drought-responsive proteins (DRPs) suggested an induction of proteins related to cell wall biogenesis, ATP synthesis, photosynthesis, and carbohydrate/energy metabolism in leaves under stress condition. A large fraction of root proteins were identified to be involved in defense and oxidative stress response. In addition, a significant change was detected in proteins related to protein synthesis, ATP synthesis, and germin-like proteins in response to drought stress. A detailed analysis of this data may be obtained from Ref. [1]

Usefulness of Danaparoid sodium in patients with Heparin-induced thrombocytopenia after cardiac surgery

Objective: Thrombocytopenia is a common problem in cardiovascular surgery patients. However, heparin-induced thrombocytopenia (HIT) is a rare but life-threatening complication of prophylaxis or treatment with heparin. Prompt management of HIT with an alternative anticoagulant is necessary due to the extreme risk of thrombotic complications. Therefore, we evaluated the effects of danaparoid in the treatment of HIT in patients with cardiac surgery who are at moderate to high risk of HIT. Methods: A prospective observational study involving 418 postcardiac surgery patients who received unfractionated heparin and low-molecular weight heparin was conducted in an educational tertiary cardiac care hospital in Iran. All patients were assessed for HIT type II based on thrombocytopenia and pretest clinical scoring system, the "4T′s" score. HIT patients were treated with 1500-2500 units intravenous bolus danaparoid sodium followed by 200-400 units/h for a mean of 5 days. Successful response to danaparoid therapy, defined as augmentation in platelet count and improvement of thrombotic events was assessed in all patients treated with danaparoid. Findings: According to pretest clinical score (4T′s), the probability of HIT was high in 14 (3.3%) patients and intermediate in three ones (0.7%). 15 patients with HIT were treated with danaparoid. One death occurred in danaparoid-treated group due to persistent thrombocytopenia. The rest of patients were treated successfully with danaparoid without any major thrombotic complication. Conclusion: According to our data and the previous studies′, HIT can be managed prosperously with danaparoid in postcardiac surgery patients. However, with the absence of any increase in platelet count after 3-5 days of danaparoid therapy and/or the occurrence of a new thrombotic event, danaparoid cross-reactivity with heparin should be suspected

The Cmobined Effect of Caffeic Acid and Low-Power Laser on Wound Healing in Male Wistar Rats

BACKGROUND AND OBJECTIVE: Wound healing is a complex, regular, and dynamic response to damage, involving interactions between different cell types, structural proteins, growth factors, and proteinase. Caffeic acid for years, and laser since the late 60s have been used for the treatment of skin conditions. This study aimed to review the combined effect of caffeic acid and laser on wound healing in male Wistar rats. METHODS: In this experimental study, 36 male Wistar rats, weighing 200-250 g, were divided into four groups of 9 rats. The control group received no interventions, the second group was treated by laser, the third group received caffeic acid, and the fourth group was treated by both caffeic acid and laser. The laser-treated groups were locally treated by laser and caffeic acid after ulcer formation. On days 4, 7, and 10 after the intervention, the wound area was measured. For histological evaluation, tissue samples were obtained and the amount of hydroxyproline in urine samples was measured on day 10 via biochemical methods afterwards, the groups were compared. FINDINGS: The obtained results showed that the percentage of wound healing on days 7 and 10 in all treatment groups (groups 1, 2, and 3) was more significant than that reported in the control group. Measurment of hydroxyproline showed increased concentration in the second (8.9±0.4), third (8.3±0.29), and fourth (9.3±0.27) groups, unlike the control group (7±0.36) (p<0.05). CONCLUSION: In this research, the wound healing effect of caffeic acid and laser, specifically their combination, was confirmed by histological studies and biochemical evaluations moreover, hydroxyproline concentration increased in all treatment groups

Rutin activates the MAPK pathway and BDNF gene expression onbeta-amyloid induced neurotoxicity in rats

Flavonoids are present in foods such as fruits and vegetables. A relationship between the consumption offlavonoid-rich foods and prevention of human disease including neurodegenerative disorders has beendemonstrated. We assessed the effect of rutin (3,3�,4�,5,7-pentahydroxyflavone-3-rhamnoglucoside) onthe mitogen-activated protein kinase (MAPK) pathway, memory retrieval and oxidative stress in ratsinjected with �-amyloid (A�), which is implicated to have an important role in Alzheimer’s disease (AD).A� was injected bilaterally in the deep frontal cortex of rat brain. Next, rutin and saline were injected(i.p.) for 3 weeks. In comparison to the control group, rutin significantly increased extracellular signal-regulated protein kinase 1 (ERK1), cAMP response element-binding protein (CREB) and brain-derivedneurotrophic factor (BDNF) gene expression in the hippocampus of rats. Rutin (100 mg/kg) significantlyincreased memory retrieval compared to the control group. Malondialdehyde (MDA) level in the hip-pocampus of the rutin group was significantly lower than those in the control group. The content ofsulfhydryl groups in the rutin group was higher than that in the control group. The findings show apossibility that rutin may have beneficial effects against neurotoxicity of A� on memory in rats

Comparing A-Mode Biometry, B-Mode Biometry and IOL Master in Measurement of Axial Length : Phaco

Background: To perform a comparison of axial length (AL) measurements using A-mode biometry, B-mode biometry and IOL Master.Material and Methods: Axial length among patients undergoing cataract surgery in Basir Eye Clinic, Tehran, Iran from May 2017 and September 2017 was determined pre-operatively using three methods. Axial length was first measured using IOLMaster 500 (Carl Zeiss Meditec, Jena, Germany), then by A-mode biometry using immersion technique and finally using horizontal axial B-scan immersion biometry. The interval between each examination was 5 minutes.Results: Statistical analysis revealed strong correlations between AL measurement values obtained from A-mode and B-mode (r = 0.983, p &lt; 0.001), A mode and IOLMaster (r = 0.999, p &lt; 0.001) as well as B-mode and IOLMaster (r = 0.984, p &lt;0.001). All correlations were strong but the strongest correlation was observed between A mode and IOLMaster methods.Conclusion: A-mode biometry showed a stronger correlation with IOL Master AL readings compared to B-Mode, thus when there are limited resources and the optical method is not accessible, A-mode immersion echography is proffered as an alternative to IOLMaster.Keywords: Biometry; IOLMaster; Axial Length; Comparison

Comparative physiology and proteomic analysis of two wheat genotypes contrasting in drought tolerance

Comparative physiology and proteomic analyses were conducted to monitor the stress response of two wheat genotypes (SERI M 82 (SE) and SW89.5193/kAu2 (SW)) with contrasting responses to drought stress. Under stress condition, the tolerant genotype (SE) produced higher shoot and root biomasses, longer roots and accumulated higher level of ABA in leaves. Physiological measurements suggested that the SE genotype was more efficient in water absorption and could preserve more water presumably by controlling stomata closure. Proteomic analysis showed an increased abundance of proteins related to defense and oxidative stress responses such as GLPs, GST, and SOD, and those related to protein processing such as small HSPs in roots of both genotypes in response to drought stress. Interestingly, the abundance of proteins such as endo-1,3-beta-glucosidase, peroxidase, SAMS, and MDH significantly increased in roots or leaves of the SE genotype and decreased in that of the SW one. In addition, an increased abundance of APX was detected in leaves and roots of the SE genotype and a decreased abundance of 14-3-3 and ribosomal proteins were noted in the SW one in response to drought stress. Our findings led to a better understanding about the integrated physiology and proteome responses of wheat genotypes with nearly contrasting responses to drought stress. Biological significance: We applied a comparative physiology and proteomic analysis to decipher the differential responses of two contrasting wheat genotypes to drought stress. Based on physiological measurements the tolerant genotype (SE) showed better drought response by developing deep root system, higher root and shoot biomasses, and higher level of ABA in leaves. Proteomic analysis showed an increased abundance of proteins related to defense and oxidative stress responses such as GLPs, GST, and SOD, and those related to protein processing such as small HSPs in roots of both genotypes in response to drought stress. In addition, the abundance of proteins such as glucan endo-1,3-beta-glucosidase, peroxidases, SAMS, and MDH increased in roots or leaves of the tolerant genotype (SE) and decreased in that of the sensitive genotype (SW). Overall, proteins related to oxidative stress, protein processing and photosynthesis showed decreased abundance to a greater extent in the sensitive genotype (SW).15 page(s