157 research outputs found
Changes in the Amphibian Antibody Repertoire are Correlated With Metamorphosis and not With Age or Size
Tadpole and adult Xenopus, manipulated to be of comparable size, exhibited stagespecific
antibody expression. The production of adult-type higher-affinity anti-DNP
antibodies proved to be independent of the age and size of the individual and is
concomitant with the completion of metamorphosis. The appearance of new antibody
specificities at such a time suggests that their expression occurs with the cell turnover
and renewal during a period of morphological changes
Expression of MHC Class II Antigens During Xenopus Development
Larval and adult forms of the amphibian Xenopus differ in their MHC class II .expression. In
tadpoles, class II epitopes can be detected by monoclonal antibodies only on B cells, macrophages
(whatever their location), spleen reticulum, thymus epithelium, and the pharyngobuccal
cavity. In contrast, all adult T cells express class II on their surface. The transitions in
class II expression occur at metamorphosis and are accompanied by other changes. The skin
is invaded by class II positive dendritic cells, and the skin glands differentiate and also
express class II. The gut, which expressed class II in discrete areas of the embryonic tissue,
becomes invaded with B cells, and its epithelium also becomes class II positive
Somatic Mutations During an Immune Response in Xenopus Tadpoles
The tadpole B-cell repertoire is less diverse than that of the adult frog; their antibodies are
of lower affinity and are less heterogenous. In order to determine whether this difference
is due to a lack of or a reduced rate of somatic hypermutation, we analyzed and compared
cDNA sequences utilizing VH1 elements with germline counterparts in isogenic LG7
tadpoles during an immune response. Indeed, tadpole VH1 sequences contained somatic
mutations. There were zeo to 5 mutations per sequence, all single base-point mutations,
with the high ratio of GC to AT base-pair alterations similar to that observed in adult frogs
Lymphoid Tumors of Xenopus laevis with Different Capacities for Growth in Larvae and Adults
Three new lymphoid tumors offering an assortment of variants in terms of MHC class I
expressions, MHC class II expression, and Ig gene transcription have been discovered in the
amphibian Xenopus. One was developed in an individual of the isogenic LG15 clone
(LG15/0), one in a frog of the LG15/40 clone (derived from a small egg recombinant of
LG15), and one (ff-2) in a male ff sib of the individual in which MAR1, the first lymphoid
tumor in Xenopus was found 2 years ago. These tumors developed primarily as thymus
outgrowths and were transplantable in histocompatible tadpoles but not in nonhistocompatible
hosts. Whereas LG15/0 and LG15/40 tumor cells also grow in adult LG15 frogs, the
ff-2 tumor, like the MAR1 cell line, is rejected by adult ff animals. Using flow cytometry with
fluorescence-labeled antibodies and immunoprecipitation analysis, we could demonstrate
that, like MAR1, these three new tumors express on their cell surface lymphopoietic
markers recognized by mAbs FIF6 and RC47, as well as T-cell lineage markers recognized
by mAbs AM22 (CD8-1ike) and X21.2, but not by immunologobulin (Ig) nor MHC class II
molecules. Another lymphocyte-specific marker AM15 is expressed by 15/0 and 15/40 but
not ff-2 tumor cells. The ff-2 tumor cell expresses MHC class molecule in association with
β2-microglobulin on the surface, 15/40 cells contain cytoplasmic I α chain that is
barely detected at the cell surface by fluocytometry, and 15/0 cells do not synthesize class
I α chain at all. The three new tumors all produce large amounts of IgM mRNA of two
different sizes but no Ig protein on the membrane nor in the cytoplasm. All tumor cell types
synthesize large amount of Myc mRNA and MHC class I-like transcripts considered to be
non classical
Sequences of CÎĽ and the VH1 Family in LG7, a Clonable Strain of Xenopus, Homozygous for the Immunoglobulin Loci
Twenty-eight heavy-chain variable (VH1) region genes and the immunoglobulin (IgM)
heavy-chain constant region of an isogenic Xenopus hybrid, X. laevis/X, gilli, LG7, have
been sequenced. The LG7 clone represents the first Xenopus hybrid that is homozygous
for the IgH locus. The VH1 family was specifically investigated because VH1 genes are
used by the antibodies produced during the Xenopus antidinitrophenol (DNP) response,
These VH1 germ-line sequences establish a so-called ”dictionary— that is available for
studying somatic hypermutational mechanisms in immunized frogs
Immunological memory: What's in a name?
Immunological memory is one of the core topics of contemporary immunology. Yet there are many discussions about what this concept precisely means, which components of the immune system display it, and in which phyla it exists. Recent years have seen the multiplication of claims that immunological memory can be found in "innate" immune cells and in many phyla beyond vertebrates (including invertebrates, plants, but also bacteria and archaea), as well as the multiplication of concepts to account for these phenomena, such as "innate immune memory" or "trained immunity". The aim of this critical review is to analyze these recent claims and concepts, and to distinguish ideas that have often been misleadingly associated, such as memory, adaptive immunity, and specificity. We argue that immunological memory is a gradual and multidimensional phenomenon, irreducible to any simple dichotomy, and we show why adopting this new view matters from an experimental and therapeutic point of view
A Large Repertoire of Parasite Epitopes Matched by a Large Repertoire of Host Immune Receptors in an Invertebrate Host/Parasite Model
For many decades, invertebrate immunity was believed to be non-adaptive, poorly specific, relying exclusively on sometimes multiple but germ-line encoded innate receptors and effectors. But recent studies performed in different invertebrate species have shaken this paradigm by providing evidence for various types of somatic adaptations at the level of putative immune receptors leading to an enlarged repertoire of recognition molecules. Fibrinogen Related Proteins (FREPs) from the mollusc Biomphalaria glabrata are an example of these putative immune receptors. They are known to be involved in reactions against trematode parasites. Following not yet well understood somatic mechanisms, the FREP repertoire varies considerably from one snail to another, showing a trend towards an individualization of the putative immune repertoire almost comparable to that described from vertebrate adaptive immune system. Nevertheless, their antigenic targets remain unknown. In this study, we show that a specific set of these highly variable FREPs from B. glabrata forms complexes with similarly highly polymorphic and individually variable mucin molecules from its specific trematode parasite S. mansoni (Schistosoma mansoni Polymorphic Mucins: SmPoMucs). This is the first evidence of the interaction between diversified immune receptors and antigenic variant in an invertebrate host/pathogen model. The same order of magnitude in the diversity of the parasite epitopes and the one of the FREP suggests co-evolutionary dynamics between host and parasite regarding this set of determinants that could explain population features like the compatibility polymorphism observed in B. glabrata/S. mansoni interaction. In addition, we identified a third partner associated with the FREPs/SmPoMucs in the immune complex: a Thioester containing Protein (TEP) belonging to a molecular category that plays a role in phagocytosis or encapsulation following recognition. The presence of this last partner in this immune complex argues in favor of the involvement of the formed complex in parasite recognition and elimination from the host
Dscam1 in pancrustacean immunity: current status and a look to the future
The Down syndrome cell adhesion molecule 1 (Dscam1) gene is an extraordinary example of diversity: by combining alternatively spliced exons, thousands of isoforms can be produced from just one gene. So far, such diversity in this gene has only been found in insects and crustaceans, and its essential part in neural wiring has been well-characterized for Drosophila melanogaster. Ten years ago evidence from D. melanogaster showed that the Dscam1 gene is involved in insect immune defense and work on Anopheles gambiae indicated that it is a hypervariable immune receptor. These exciting findings showed that via processes of somatic diversification insects have the possibility to produce unexpected immune molecule diversity, and it was hypothesized that Dscam1 could provide the mechanistic underpinnings of specific immune responses. Since these first publications the quest to understand the function of this gene has uncovered fascinating insights from insects and crustaceans. However, we are still far from a complete understanding of how Dscam1 functions in relation to parasites and pathogens and its full relevance for the immune system. In this Hypothesis and Theory article, we first briefly introduce Dscam1 and what we know so far about how it might function in immunity. By focusing on seven questions, we then share our sometimes contrasting thoughts on what the evidence tells us so far, what essential experiments remain to be done, and the future prospects, with the aim to provide a multiangled view on what this fascinating gene has to do with immune defense
Origin of Immunoglobulin Isotype Switching
SummaryBackgroundFrom humans to frogs, immunoglobulin class switching introduces different effector functions to antibodies through an intrachromosomal DNA recombination process at the heavy-chain locus. Although there are two conventional antibody classes (IgM, IgW) in sharks, their heavy chains are encoded by 20 to >100 miniloci. These representatives of the earliest jawed vertebrates possess a primordial immunoglobulin gene organization where each gene cluster is autonomous and contains a few rearranging gene segments (VH-D1-D2-JH) with one constant region, μ or ω.ResultsV(D)J rearrangement always takes place within the μ cluster, but here we show that the VDJ can be expressed with constant regions from different clusters, although IgH genes are spatially distant, at >120 kb. Moreover, reciprocal exchanges take place between Igω and Igμ genes. Switching is augmented with deliberate immunization and is concomitant with somatic hypermutation activity. Because switching occurs independently of the partners' linkage position, some events involve transchromosomal recombination. The switch sites consist of direct joins between two genes in the 3′ intron flanking JH.ConclusionsOur data are consistent with a mechanism of cutting or joining of distal DNA lesions initiated by activation-induced cytidine deaminase (AID), in the absence of mammalian-type switch regions. We suggest that, in shark, with its many autonomous IgH targeted by programmed DNA breakage, factors predisposing broken DNA ends to translocate configured the earliest version of class switch recombination
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