Bioreactivity, Guttation and Agents Influencing Surface Tension of Water Emitted by Actively Growing Indoor Mould Isolates

The secretion of metabolites in guttation droplets by indoor moulds is not well documented. This study demonstrates the guttation of metabolites by actively growing common indoor moulds. Old and fresh biomasses of indoor isolates of Aspergillus versicolor, Chaetomium globosum, Penicillium expansum, Trichoderma atroviride, T. trixiae, Rhizopus sp. and Stachybotrys sp. were compared. Metabolic activity indicated by viability staining and guttation of liquid droplets detected in young (6 months old) cultures consisting of dehydrated hyphae and dormant conidia. Fresh

PALAEORUNOFF FROM LAKE LADOGA TO THE BALTIC SEA DURING THE HOLOCENE

The article presents the results of the palaeohydrological analysis of the river system reorganization in the North-West of Russia, the Karelian Isthmus. This study aimed at the hydrological calculation of the runoff through the Heinjoki water connection of Lake Ladoga, the Gulf of Finland and PalaeoVuoksa during different periods of the Holocene. The methods of the equation of water balance, hydraulic-morphometric dependencies and the method of geographical analogy were used in this research. The main result of the carried work is the refinement of the existing regional flow model. In particular, the role of the Heinjoki Strait and the Neva River channel in different stages of the Holocene is considered. The authors came to the conclusion that Lake Ladoga always had a runoff to the Baltic Sea. It is shown that most probably in the period of 10,200-3,500 years ago the runoff was carried out through the Heinjoki waterway. The study underlines that the isostatic factor played a significant role in the forming of the water flow. The change in the runoff direction from the Heinjoki waterway to the Neva River is mainly related to the isostatic uplift of the Karelian Isthmus and the northern part of Lake Ladoga. Quantitative parameters of the Heinjoki palaeoflow (flow rate, annual runoff, runoff layer, runoff coefficient) seem to be roughly equivalent to the modern parameters of the flow of the Neva River. The article is dedicated to our colleague, a talented geographer and teacher, PhD Oleg Borisovich Averichkin (1979-2010) who first performed the calculations of the paleorunoff of Lake LadogaThe article presents the results of the palaeohydrological analysis of the river system reorganization in the North-West of Russia, the Karelian Isthmus. This study aimed at the hydrological calculation of the runoff through the Heinjoki water connection of Lake Ladoga, the Gulf of Finland and PalaeoVuoksa during different periods of the Holocene. The methods of the equation of water balance, hydraulic-morphometric dependencies and the method of geographical analogy were used in this research. The main result of the carried work is the refinement of the existing regional flow model. In particular, the role of the Heinjoki Strait and the Neva River channel in different stages of the Holocene is considered. The authors came to the conclusion that Lake Ladoga always had a runoff to the Baltic Sea. It is shown that most probably in the period of 10,200-3,500 years ago the runoff was carried out through the Heinjoki waterway. The study underlines that the isostatic factor played a significant role in the forming of the water flow. The change in the runoff direction from the Heinjoki waterway to the Neva River is mainly related to the isostatic uplift of the Karelian Isthmus and the northern part of Lake Ladoga. Quantitative parameters of the Heinjoki palaeoflow (flow rate, annual runoff, runoff layer, runoff coefficient) seem to be roughly equivalent to the modern parameters of the flow of the Neva River. The article is dedicated to our colleague, a talented geographer and teacher, PhD Oleg Borisovich Averichkin (1979-2010) who first performed the calculations of the paleorunoff of Lake Ladog

Problems of personnel irradiation in modern medical technologies

BACKGROUND: The widespread use of radiation sources in medical practice (cardio-endovascular surgery, endoscopy, traumatology, urology, neurosurgery, dentistry, and radioisotope diagnostics departments) leads to irradiation of the lens of the eye and the skin of the hands. The introduction of new recommendations by the IAEA to reduce the limit of the annual equivalent dose to the lens (20 mSv) has led to an inaccurate dose assessment based on the effective dose. AIM: To analyze approaches and assess equivalent doses of irradiation of the lens of the eye and skin of the hands of medical personnel during various diagnostic studies under the influence of X-rays and radiopharmaceuticals studies and to compare the results obtained with previously published data. MATERIALS AND METHODS: Thermo-luminescent dosimetry was used. Dose assessment was performed by cardio-endovascular surgery, endoscopy, isotope diagnostics, dentistry, and urology personnel. RESULTS: The estimated annual equivalent doses to the lens of the eye for doctors of cardio-endovascular surgery departments, in most cases, ranging 3590 mSv, 619 mSv for the average medical staff (in some cases, the doctor [225 mSv] and the nurse [180 mSv]) and 4.59 mSv for the staff of the department of radioisotope diagnostics. The annual calculated equivalent doses to the skin of the hands for cardio-endovascular surgery personnel were 17100 and 24220 mSv for the staff working with radiopharmaceuticals. It is shown that the use of an estimate of the average dose per operation by cardio-endovascular surgery doctors, as a rule, inevitably leads to an excess of the equivalent dose to the lens of the eye after a certain number of operations. CONCLUSION: When a certain number of operations are exceeded (100200), equivalent doses to the eyes lens in cardio-endovascular surgery doctors above 20 mSv per year can be formed. At current radiation levels, a lesion of the eyes lens was found in a cardio-endovascular surgery doctor. The results indicate the need for further dosimetric measurements and epidemiological studies, based on which recommendations for radiation protection of the eyes lens and the skin of the hands of medical personnel working in low-intensity, scattered, gamma X-ray radiation can be developed

Comparative Genomic Analysis of Human Fungal Pathogens Causing Paracoccidioidomycosis

Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. In addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts.National Institute of Allergy and Infectious Diseases (U.S.)National Institutes of Health. Department of Health and Human Services (contract HHSN266200400001C)National Institutes of Health. Department of Health and Human Services(contract HHSN2722009000018C)Brazil. National Council for Scientific and Technological Developmen

Parameter Estimation and Supervisory Techniques for Robust Longitudinal Control of Heavy Vehicles

This report describes the development and experimental validation of a coordination scheme between friction and discretely variable compression brakes for a Class 8 Freightliner truck used as a development platform in the California PATH program. The coordination scheme that we developed maintains the speed tracking performance of the nominal PID controller which was originally designed by the UCLA team for the case of friction brakes only. Through the coordination scheme, the command of the nominal PID controller is split into a friction brake command and compression brake command. The integration of the compression brake into the speed control framework reduces the usage and wear of the conventional friction brakes. This report describes the experimental hardware and software setup; open-loop experiments that were conducted to identify the models for the friction brake and the compression brake; the identification procedure of the friction brake and compression brake characteristics and the identification results; the development of the coordination scheme for the friction and compression brakes and the results of closed loop experiments that were used to validate the coordination scheme performance

Identification and Integration of Commercial Heavy Vehicle Retarders

This report describes the development and experimental validation of a coordination scheme between friction and discretely variable compression brakes for a Class 8 Freightliner truck used as a development platform in the California PATH program. The coordination scheme that we developed maintains the speed tracking performance of the nominal PID controller which was originally designed by the UCLA team for the case of friction brakes only....

Longitudinal Control of Commercial Heavy Vehicles Equipped with Variable Compression Brake

In this report, we extend our previous work within MOU 372 on modeling and longitudinal speed control design for heavy commercial vehicles equipped with variable compression braking mechanisms. Previously, we developed a detailed crankangle based simulation model for a six cylinder, 350 hp diesel engine with a continuously varying compression brake that is capable of describing the intrinsic interactions between individual cylinder intake and exhaust processes, turbocharger dynamics during combustion and braking modes, and the transition between those modes. Moreover, for control design and analysis purposes we derived a low-order approximation of that model. In this report we present our new results on developing longitudinal control algorithms that coordinate the variable compression brake with conventional service brakes and gear selection. Specifically, we integrate the compression brake actuator with the service brakes and design a PI-controller that emulates the driver's actions on long grades. The controller uses the engine speed measurement to activate the service brakes only when retarding power of the compression brake is insufficient. We also employ robust linear control technique using the concept of structured singular values to design a controller that is robust to parameter variations and model uncertainty in the CHV. Finally, we compare the performance of this robust controller with the performance of an adaptive control scheme that we have derived within MOU 393. The performance of all the controllers is demonstrated through extensive simulations on the 24th order nonlinear vehicle model developed in our previous work within MOU 372