Making Fenians: The Transnational Constitutive Rhetoric of Revolutionary Irish Nationalism, 1858-1876

This dissertation traces the constitutive rhetorical strategies of revolutionary Irish nationalists operating transnationally from 1858-1876. Collectively known as the Fenians, they consisted of the Irish Republican Brotherhood in the United Kingdom and the Fenian Brotherhood in North America. Conceptually grounded in the main schools of Burkean constitutive rhetoric, it examines public and private letters, speeches, Constitutions, Convention Proceedings, published propaganda, and newspaper arguments of the Fenian counterpublic. It argues two main points. First, the separate national constraints imposed by England and the United States necessitated discursive and non-discursive rhetorical responses in each locale that made it near impossible to sustain transnational consubstantiality for the movement. Second, North American Fenian strategies to gain sovereign recognition for Ireland relied on and helped to further substantiate the palliative Constitutional wishes of equality that undergirded the racial and settler inequalities of the United States. After establishing the exigency and framework for the project, Chapter 2 examines the transnational attempts by Fenian leadership to constitute the Irish nation in the diaspora across existing national borders. It argues that, despite the shared vision and motives, the separate national constraints negotiated by each arm of the movement made it impossible to maintain a shared strategy for achieving Irish freedom. Chapter 3 then focuses on the Constitutions created by the North American organization in order to constitute Irish sovereignty, demonstrating how the scenic conditions wrought by these Constitutional enactments contributed to a legitimacy crisis that led to the schism in the Fenian Brotherhood and paved the way for multiple failed invasions of Canada. Chapter 4 examines the constitutive rhetorical strategies of The Fenians\u27 Progress, a propaganda tract used by the wing that sought to invade Canada, and limns the rhetorics of respectability this faction employed as they appealed to the U.S. for recognition of Fenian belligerent status. Chapter 5 juxtaposes the rhetorics of skirmishing and settling in The Irish World in the mid-1870s in the wake of the failed Canadian invasions, tracing the rhetorics of settler solidarity these otherwise anti-imperialist Irish-Americans invoke in print. It concludes by discussing the Fenian case\u27s implications for rhetorical theory

Using numerical methods to simulate Newtonian mechanics in video games

Using numerical methods to simulate Newtonian mechanics in video games An exploration into the physics of video games by implementing numerical methods through programming. There are several methods to consider when developing a physical system for a game, for instance Euler\u27s method is an efficient method, which unfortunately is rarely viable. There also exist a few variations to Euler\u27s method that give more functionality, such as semi-implicit Euler and implicit Euler. Each has different restrictions and advantages, but that not to say we only explore Euler\u27s and its variations. Verlet integration is a useful method to calculate trajectories in a multiple particle system, which been utilized across many fields. Lastly we will study RK4, Runge-Kutta 4, a method developed by two German mathematicians. RK4 is by no means a perfect numerical method, but is a good general purpose integration technique. We investigate single-particle systems in two dimensions, and note the similarity to multi-particle systems in two dimensions. Adding a third dimension greatly increases the complexity of the simulation , we simply touch on the topic for today. A less formal discussion of a few important considerations in game development takes place, topics include varying refresh rates and PC specifications (the game should run the same for everyone, every time it is executed), and how communicating with a server introduces much more complexity

Information Technology Use by Local Governments in the Northeast: Assessment and Needs

How local governments are using information technology is important to understand if extension teaching on local government and economic development issues is to be effective. This study uses results from surveys of local officials in New York, Pennsylvania, and West Virginia to examine the potential for delivering extension programs to local officials through information technology. The responses suggest that local officials predominantly prefer face-to-face training, but many are willing to try distance education. In addition, even though many governments are using the Internet, there still are significant numbers who do not yet have access to these technologies

Allosteric activation of the 5-HT_3AB receptor by mCPBG

The 5-HT_3AB receptor contains three A and two B subunits in an A-A-B-A-B order. However, serotonin function at the 5-HT_3AB receptor has been shown to depend solely on the A-A interface present in the homomeric receptor. Using mutations at sites on both the primary (E122) and complementary (Y146) faces of the B subunit, we demonstrate that meta-chlorophenyl biguanide (mCPBG), a 5-HT_3 selective agonist, is capable of binding to and activating the 5-HT_3AB receptor at all five subunit interfaces of the heteromer. Further, mCPBG is capable of allosterically modulating the activity of serotonin from these sites. While these five binding sites are similar enough that they form to a monophasic dose – response relationship, we uncover subtle differences in the heteromeric binding sites. We also find that the A-A interface appears to contribute disproportionately to the efficacy of 5-HT_3AB receptor activation

The 5-HT3AB Receptor Shows an A3B2 Stoichiometry at the Plasma Membrane

AbstractThe 5-HT3AB receptor is the best-characterized heteropentameric 5-HT3 receptor. Under conditions of heterologous expression, the 5-HT3AB receptor shows a single functionally resolvable population, suggesting the presence of a unique subunit stoichiometry; however, conflicting previous reports have suggested two different possible stoichiometries. Here we isolate plasma membrane sheets containing assembled receptors from individual HEK293T cells. We then determine the stoichiometry of 5-HT3AB receptors on the plasma membrane by fluorescence methods, employing meCFP- and meYFP-labeled A and B subunits. Over a wide range of cDNA transfection ratios, fluorescence intensity ratios are closest to values that correspond to a subunit ratio of A3B2. Förster resonance energy transfer (family FRET) efficiencies provide minor corrections (3–6%) to the subunit ratios and provide independent support for a predominantly A3B2 stoichiometry on the plasma membrane sheets. Twin FRET efficiencies support these data, also suggesting that the two B subunits are nonadjacent in most of the heteropentamers. The high-frequency variant HTR3B p.Y129S (c.386A>C, rs11767445), linked to psychiatric disease, also forms A3B2 receptors on the plasma membrane. The 5-HT3B Y129S, subunit incorporates in a slightly (11–14%) more efficient manner than the common variant. In general, most of the subunits reside within the cell. In contrast to the findings for the plasma membrane, the relative abundances and FRET characteristics of intracellular subunits depend strongly on the transfection ratio. The straightforward and unambiguous combination of plasma membrane-sheet isolation, fluorescence intensity ratios, and FRET is a generally promising procedure for determining stoichiometry of proteins on the plasma membrane

Probing Binding Interactions of Cytisine Derivatives to the α4β2 Nicotinic Acetylcholine Receptor

Nicotinic acetylcholine receptors (nAChRs) are crucial for communication between synapses in the central nervous system. As such, they are also implicated in several neuropsychiatric and addictive diseases. Cytisine is a partial agonist of some nAChRs and has been used for smoking cessation. Previous studies have established a binding model for several agonists to several nAChR subtypes. Here, we evaluate the extent to which this model applies to cytisine at the α4β2 nAChR, which is a subtype that is known to play a prominent role in nicotine addiction. Along with the commonly seen cation−π interaction and two hydrogen bonds, we find that cytisine makes a second cation−π interaction at the agonist binding site. We also evaluated a series of C(10)-substituted cytisine derivatives, using two-electrode voltage-clamp electrophysiology and noncanonical amino acid mutagenesis. Double-mutant cycle analyses revealed that C(10) substitution generally strengthens the newly established second cation−π interaction, while it weakens the hydrogen bond typically seen to LeuE in the complementary subunit. The results suggest a model for how cytisine derivatives substituted at C(10) (as well as C(9)/C(10)) adjust their binding orientation, in response to pyridone ring substitution