Whipple’s Disease: Our Own Experience and Review of the Literature

Whipple’s disease is a chronic infectious systemic disease caused by the bacterium Tropheryma whipplei. Nondeforming arthritis is frequently an initial complaint. Gastrointestinal and general symptoms include marked diarrhoea (with serious malabsorption), abdominal pain, prominent weight loss, and low-grade fever. Possible neurologic symptoms (up to 20%) might be associated with worse prognosis. Diagnosis is based on the clinical picture and small intestinal histology revealing foamy macrophages containing periodic-acid-Schiff- (PAS-) positive material. Long-term (up to one year) antibiotic therapy provides a favourable outcome in the vast majority of cases. This paper provides review of the literature and an analysis of our 5 patients recorded within a 20-year period at a tertiary gastroenterology centre. Patients were treated using i.v. penicillin G or amoxicillin-clavulanic acid + i.v. gentamicin for two weeks, followed by p.o. doxycycline (100 mg per day) plus p.o. salazopyrine (3 g per day) for 1 year. Full remission was achieved in all our patients

Body indices and basic vital signs in Helicobacter pylori positive and negative persons

It has been hypothesized that Helicobacter pylori (Hp) infection may contribute to reduced stature, risk of hypertension or obesity. The aim was to evaluate body indices in Hp positive and negative persons. A total of 2436 subjects (4–100 years old) were tested for Hp status by 13Curea breath test. Data on height and weight were collected for 84%, and blood pressure for 80% of the study subjects. The prevalence of Hp infection was 41.6%. The odds ratio for a 10-year increase in age was 1.21 (95% CI 1.17–1.25, p-value <0.001). Statistically significant negative association of Hp positivity with body height was most pronounced in the younger age groups, while a positive association of Hp positivity with body mass index was only seen in those aged 15+ years. There was a negative effect of Hp positivity on systolic and diastolic blood pressure in subjects below 25 and a relatively strong positive effect on blood pressure in subjects over 65 years. Residual confounding by social characteristics as a possible explanation for the associations of Hp positivity with height and blood pressure cannot be excluded. Unmeasured factors related to social and family environment may cause the apparent association between Hp positivity and children’s growth and blood pressure

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Development of alder carr after the abandonment of wet grasslands during the last 70 years

• The secondary succession of wet grasslands to communities of alder carr dominated by Alnus glutinosa was recorded in different parts of Europe during the 20th century. However, knowledge of such development of alder carr remains insufficient. • The development of alder carr was reconstructed at five sites in the Czech Republic, using historical aerial photographs and methods of dendrochronology. The aims were to investigate the succession from wet grasslands to alder carr at sites previously intensively managed for agriculture and to find out the role of fluctuations in the groundwater table, caused by artificial drainage channels, in the observed stand dynamics and tree growth. • The spread of forest (i.e., an increase in forest cover) predominated until the 1970s at all sites. This trend was disrupted by a large-scale dieback of forest stands in four of the five sites after the 1970s, followed by an increase in patch heterogeneity, as indicated by landscape metrics. The radial growth increment in Alnus glutinosa has been affected predominately by local environmental factors, probably including the changing degree of waterlogging. Forest dieback was presumably connected with a lesser extent of drainage channels. • Our results indicate that observed successional pathways at sites of alder carr were probably caused by local changes in the groundwater table.Développement de taillis d'aunes après l'abandon des prairies humides au cours des 70 dernières années. • La succession secondaire des prairies humides en communautés de taillis d'aunes dominées par Alnus glutinosa a été enregistrée dans différentes parties de l'Europe au cours du 20e siècle. Toutefois, la connaissance du développement de taillis d'aunes reste insuffisante. • Le développement de taillis d'aunes a été reconstruit dans cinq sites en République Tchèque, à l'aide de photographies aériennes historiques et des méthodologies de la dendrochronologie. Les objectifs étaient d'étudier la succession de prairies humides en taillis d'aunes sur des sites précédemment exploités intensivement par l'agriculture et de découvrir le rôle des fluctuations de la nappe phréatique, causées par le drainage artificiel des canaux, et d'observer la dynamique des peuplements et la croissance des arbres. • La progression de la forêt (c'est-à-dire, l'augmentation du couvert forestier) a prédominé jusque dans les années 1970 dans tous les sites. Cette tendance a été perturbée par un vaste dépérissement des peuplements forestiers dans quatre des cinq sites après les années 1970, suivie par une augmentation de l'hétérogénéité des bouquets d'arbres, comme indiqué par les paramètres du paysage. L'augmentation de la croissance radiale de Alnus glutinosa a été affectée principalement par les facteurs environnementaux, y compris sans doute l'évolution du degré d'engorgement. Le dépérissement des forêts est probablement lié à une moindre importance des canaux de drainage. • Nos résultats indiquent que les voies de succession observées sur les sites des taillis d'aunes ont probablement été causées par des changements locaux dans la nappe phréatique

Analyses of Hybrid Viability across a Hybrid Zone between Two Alnus Species Using Microsatellites and cpDNA Markers

Diploid Alnus glutinosa s. str. and autotetraploid A. rohlenae form a narrow hybrid zone in a study area in southern Serbia, which results in triploid hybrid formation. The vast majority of previous studies have been focused on studies of maternal plants, but the offspring resulting from their crossing have not been much studied. Here, we use the variability of microsatellites and chloroplast DNA between these species and their putative hybrids to create an overall picture of the development of the hybrid zone and its predicted type. To elucidate the gene transfer within both species, the origins of individual ploidies and especially the role of triploid hybrids, a germination experiment was carried out linked with a flow cytometry study of the resulting seedlings. The tension zone model seems to offer the most adequate explanation of our observations, with selection against triploid hybrids and the spatial positioning of the hybrid zone. Despite selection against them, the triploid hybrids play an important role in the exchange of genes between the two species and therefore serve as a bridge for introgression. The presence of fertile triploids is essential for enriching the haplotype diversity between these species and for the development of new genetic lineages

Data from: Traditional forest management practices stop forest succession and bring back rare plant species

Past management practices may continue to influence ecosystem functions and processes for decades, centuries or even longer after they have been abandoned. Until now, few researchers have attempted experiments which test the effects of restoring some of these past management practices on long-term community developmental trajectories. Strong evidence indicates that the diversity of various taxonomic groups declined in European lowland forests in the second half of the 20th century, following the abandonment of some traditional forest management practices. We carried out a five-year field trial in a lowland thermophilous oak forest to describe the effects of restoration of litter raking and grass cutting on the long-term developmental trajectory of species composition and the diversity of understorey vegetation. We used target species groups, i.e. species specific to thermophilous forests and dry grassland vegetation and plant functional traits to evaluate the restoration success using both compositional and functional outcomes. Dissimilarity in species composition between the managed plots and controls increased significantly during the five years of the trial. Litter raking increased the richness and cover of the thermophilous forest and dry grassland species, whereas grass cutting increased the richness and cover of only the dry grassland species. We did not record any overall trends in divergence of functional trait composition between managed and control plots. In the litter raking plots, we recorded increases in community-weighted means of specific leaf area, in lateral spread and in Ellenberg indicator values for nutrients and soil reaction. In contrast, we found little evidence for predictability of species functional trait composition subjected to grass cutting. Synthesis and applications. Following the restoration of traditional forest management practices we were able to change the community's developmental trajectory towards higher richness and cover of target species. The evaluation of community developmental trajectories using species and functional trait composition supports the idea that restoration of traditional forest management practices should carefully distinguish among possible interventions

Distribution and basic characteristics of species under study.

<p>Distribution and basic characteristics of species under study.</p

Data from: On the road: postglacial history and recent expansion of the annual Atriplex tatarica in Europe

Aim: The Holocene history of annual plant species is at best shadowy because, for most, the palaeobotanical data is scarce or absent. Hence, there is limited information on their glacial refugia and postglacial colonisation pathways. Also, little is known how human activity has affected their expansion. Here, we outline the joint influences of postglacial colonisation and recent expansions on the genetic diversity of the continental, sub-halophyte species Atriplex tatarica during the late Pleistocene and the Holocene. Location: Europe. Taxon: Atriplex tatarica (Amaranthaceae) Methods: We analysed 780 individuals from 80 populations throughout the current European distribution range, employing chloroplast DNA sequences and microsatellite markers. Results: Five haplotype lineages were recognised based on the results of the cpDNA phylogenetic analyses. These lineages originated 0.43−0.22 my BP. Bayesian clustering analyses divided populations of A. tatarica into two clusters: (i) populations in the Pannonian Basin and the Bohemian Massif and (ii) populations in the North European Plain, the Balkan Peninsula, the Carpathian Arc and the Pontic region. The ABC approach provided the strongest statistical support for a model proposing refugia in the Balkan Peninsula and the Pannonian Basin. Expansions from these refugia occurred around 7,000 yr BP. Main conclusion: Atriplex tatarica represents a continental species growing exclusively in man-made habitats. It serves as a phylogeographic model for annual ruderal taxa currently spreading in central Europe along highways and representing a large group of alien plant species, the so-called archaeophytes. Atriplex tatarica survived the last glaciation in both a southerly located Balkan refugium and in a more northerly refugium in the Pannonian Basin. From these, the species has colonised Europe as a result of expansion of humans and the anthropogenic climate change. The massive colonisation of central north Europe is very recent, with the expansion estimated to have occurred only hundreds of years ago

microsatellite_genotypes

Microsatellite genotypes for 41 populations of Atriplex tatarica