56 research outputs found

    A Complete Cross Section Data Set for Electron Scattering by Pyridine: Modelling Electron Transport in the Energy Range 0–100 eV

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    Electron scattering cross sections for pyridine in the energy range 0–100 eV, which we previously measured or calculated, have been critically compiled and complemented here with new measurements of electron energy loss spectra and double differential ionization cross sections. Experimental techniques employed in this study include a linear transmission apparatus and a reaction microscope system. To fulfill the transport model requirements, theoretical data have been recalculated within our independent atom model with screening corrected additivity rule and interference effects (IAM-SCAR) method for energies above 10 eV. In addition, results from the R-matrix and Schwinger multichannel with pseudopotential methods, for energies below 15 eV and 20 eV, respectively, are presented here. The reliability of this complete data set has been evaluated by comparing the simulated energy distribution of electrons transmitted through pyridine, with that observed in an electron-gas transmission experiment under magnetic confinement conditions. In addition, our representation of the angular distribution of the inelastically scattered electrons is discussed on the basis of the present double differential cross section experimental results

    The spike gene is a major determinant for the SARS-CoV-2 Omicron-BA.1 phenotype.

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    Variant of concern (VOC) Omicron-BA.1 has achieved global predominance in early 2022. Therefore, surveillance and comprehensive characterization of Omicron-BA.1 in advanced primary cell culture systems and animal models are urgently needed. Here, we characterize Omicron-BA.1 and recombinant Omicron-BA.1 spike gene mutants in comparison with VOC Delta in well-differentiated primary human nasal and bronchial epithelial cells in vitro, followed by in vivo fitness characterization in hamsters, ferrets and hACE2-expressing mice, and immunized hACE2-mice. We demonstrate a spike-mediated enhancement of early replication of Omicron-BA.1 in nasal epithelial cultures, but limited replication in bronchial epithelial cultures. In hamsters, Delta shows dominance over Omicron-BA.1, and in ferrets Omicron-BA.1 infection is abortive. In hACE2-knock-in mice, Delta and a Delta spike clone also show dominance over Omicron-BA.1 and an Omicron-BA.1 spike clone, respectively. Interestingly, in naĂŻve K18-hACE2 mice, we observe Delta spike-mediated increased replication and pathogenicity and Omicron-BA.1 spike-mediated reduced replication and pathogenicity, suggesting that the spike gene is a major determinant of replication and pathogenicity. Finally, the Omicron-BA.1 spike clone is less well-controlled by mRNA-vaccination in K18-hACE2-mice and becomes more competitive compared to the progenitor and Delta spike clones, suggesting that spike gene-mediated immune evasion is another important factor that led to Omicron-BA.1 dominance

    The spike gene is a major determinant for the SARS-CoV-2 Omicron-BA. 1 phenotype

    Get PDF
    Variant of concern (VOC) Omicron-BA.1 has achieved global predominance in early 2022. Therefore, surveillance and comprehensive characterization of Omicron-BA.1 in advanced primary cell culture systems and animal models are urgently needed. Here, we characterize Omicron-BA.1 and recombinant Omicron-BA.1 spike gene mutants in comparison with VOC Delta in well-differentiated primary human nasal and bronchial epithelial cells in vitro, followed by in vivo fitness characterization in hamsters, ferrets and hACE2-expressing mice, and immunized hACE2-mice. We demonstrate a spike-mediated enhancement of early replication of Omicron-BA.1 in nasal epithelial cultures, but limited replication in bronchial epithelial cultures. In hamsters, Delta shows dominance over Omicron-BA.1, and in ferrets Omicron-BA.1 infection is abortive. In hACE2-knock-in mice, Delta and a Delta spike clone also show dominance over Omicron-BA.1 and an Omicron-BA.1 spike clone, respectively. Interestingly, in naĂŻve K18-hACE2 mice, we observe Delta spike-mediated increased replication and pathogenicity and Omicron-BA.1 spike-mediated reduced replication and pathogenicity, suggesting that the spike gene is a major determinant of replication and pathogenicity. Finally, the Omicron-BA.1 spike clone is less well-controlled by mRNA-vaccination in K18-hACE2-mice and becomes more competitive compared to the progenitor and Delta spike clones, suggesting that spike gene-mediated immune evasion is another important factor that led to Omicron-BA.1 dominance

    Enabling planetary science across light-years. Ariel Definition Study Report

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    Ariel, the Atmospheric Remote-sensing Infrared Exoplanet Large-survey, was adopted as the fourth medium-class mission in ESA's Cosmic Vision programme to be launched in 2029. During its 4-year mission, Ariel will study what exoplanets are made of, how they formed and how they evolve, by surveying a diverse sample of about 1000 extrasolar planets, simultaneously in visible and infrared wavelengths. It is the first mission dedicated to measuring the chemical composition and thermal structures of hundreds of transiting exoplanets, enabling planetary science far beyond the boundaries of the Solar System. The payload consists of an off-axis Cassegrain telescope (primary mirror 1100 mm x 730 mm ellipse) and two separate instruments (FGS and AIRS) covering simultaneously 0.5-7.8 micron spectral range. The satellite is best placed into an L2 orbit to maximise the thermal stability and the field of regard. The payload module is passively cooled via a series of V-Groove radiators; the detectors for the AIRS are the only items that require active cooling via an active Ne JT cooler. The Ariel payload is developed by a consortium of more than 50 institutes from 16 ESA countries, which include the UK, France, Italy, Belgium, Poland, Spain, Austria, Denmark, Ireland, Portugal, Czech Republic, Hungary, the Netherlands, Sweden, Norway, Estonia, and a NASA contribution

    First Direct 14C Ages on Hawaiian Petroglyphs

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    We collected organics encapsulated by coatings of amorphous silica from 13 petroglyphs on Kaho'olawe Island, Hawai'i. Silica-glaze coatings can form within a few decades in Hawai'i. After backscatter electron microscopy of the overlying silica coating determined that it had been deposited in layers sequentially, organics were treated with NaOH, Hel, and HF, and were radiocarbon-dated at the New Zealand accelerator. The minimum ages obtained for these Kaho'olawe petroglyphs indicate that they span at least 80 percent of the time that the Hawaiian Islands have been occupied. Stick figures are the oldest petroglyphs, but they overlap with other linear motifs (fish hook, dog) as well as the triangular-bodied figures, which came later. KEYWORDS: Petroglyph, Hawaiian Islands, Radiocarbon Dating

    Constraining the age of the CĂŽa Valley (Portugal) engravings with radiocarbon dating

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    Formation of Silica Glaze Rock Coatings through Water Vapor Interactions

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    SHORT COMMUNICATION ANALYSIS OF GEOMORPHOLOGY CITATIONS IN THE LAST QUARTER OF THE 20TH CENTURY

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    Three hundred and twenty-eight geomorphology articles published in the last quarter of the 20th century were cited 20 or more times in Institute for Scientific Information (ISI) indices, as of 15 May 2001. At the close of the 20th century, wellcited geomorphology is highly multidisciplinary and interdisciplinary with the most dominant fields being in biological, civil engineering, earth science, geography, geological, and soils disciplines. The very strong English-language bias of well-cited journal articles creates a geographical bias in study site selection, which may in turn bias geomorphic theory. Water-based research (fluvial processes and landforms, riparian, drainage basin) dominates well-cited papers, with the ‘hottest ’ subfield in the 1990s being riparian research with a biological emphasis. Over 90 journals publish well-cited papers, but Earth Surface Processes and Landforms hosts the largest number of well-cited papers. Copyright © 2002 John Wiley & Sons, Ltd. KEY WORDS: geomorphology; landform; earth surface processes; citation; bibliometric; temporal; time; trends Garfield first proposed a citation index for analysing scientific literature (Garfield, 1955), leading to the creation of the Institute for Scientific Information (ISI). Bibliometric analyses, filtered through the ISI database

    Nanoscale Observations Support the Importance of Chemical Processes in Rock Decay and Rock Coating Development in Cold Climates

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    Conventional scholarship long held that rock fracturing from physical processes dominates over chemical rock decay processes in cold climates. The paradigm of the supremacy of cold-climate shattering was questioned by Rapp’s discovery (1960) that the flux of dissolved solids leaving a KĂ€rkevagge, Swedish Lapland, watershed exceeded physical denudation processes. Many others since have gone on to document the importance of chemical rock decay in all cold climate landscapes, using a wide variety of analytical approaches. This burgeoning scholarship, however, has only generated a few nanoscale studies. Thus, this paper’s purpose rests in an exploration of the potential for nanoscale research to better understand chemical processes operating on rock surfaces in cold climates. Samples from several Antarctica locations, Greenland, the Tibetan Plateau, and high altitude tropical and mid-latitude mountains all illustrate ubiquitous evidence of chemical decay at the nanoscale, even though the surficial appearance of each landscape is dominated by “bare fresh rock.” With the growing abundance of focused ion beam (FIB) instruments facilitating sample preparation, the hope is that that future rock decay researchers studying cold climates will add nanoscale microscopy to their bag of tools
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