24 research outputs found
ãžã³ã³ãŠ ãžã¥ã»ã€ ㎠12ãã ã¡ ã GNRH ã»ã€ã¶ã€ ãã»ã¬ãªã³ ããŠãš 㬠ãŠã· ã ãžã¥ã¿ã€ ãªã ã ãªãšãã¹ ãšã€ãã§ãŠ
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ºåºæ¿ãã«ã¢ã³æŸåºãã«ã¢ã³(GnRH : ãã»ã¬ãªã³)ã6,10,20ÎŒgæäžã,åèçã«ã€ããŠç¡åŠçœ®å¯Ÿç
§çŸ€ã®ãããšæ¯èŒæ€èšããããã®çµæ,ãã»ã¬ãªã³æäžçŸ€ã®åèçã¯å¯Ÿç
§çŸ€ã®60.0%ã«æ¯ã¹10ããã³20ÎŒgæäžçŸ€ã§ã¯ãããã72.3ããã³68.6%ãšé«ãå€ãåŸãããã次ãã§,空èæéã®çã矀(120æ¥æªæº)ãšé·ã矀(120æ¥ä»¥äž)ã®2矀ã«åããŠæ€èšãããšãã,çã矀ã®åèçã¯å¯Ÿç
§çŸ€ã®59.0%ã«æ¯ã¹ãããã®æäžçŸ€ãšãé«ãå€ãåŸãã,é·ã矀ã§ã¯å¯Ÿç
§çŸ€ã®63.2%ã«æ¯ã¹6ÎŒgæäžçŸ€ã§51.7%ãšäœãå€ã瀺ãããããã,10ããã³20ÎŒgæäžçŸ€ã§70.0ããã³69.0%ãšããããé«ãåŸåãã¿ãããããŸã,æäžåæ°ã1åãš2åã«åããŠæ€èšãããšãã,1åæäžã§ã®åèçã¯,察ç
§çŸ€ã®59.2%ã«æ¯ã¹10ããã³20ÎŒgæäžçŸ€ã§ã¯é«ãåŸåãèªããã,ç¹ã«10ÎŒgæäžçŸ€ã§ã¯73.3%ãšé«ãå€ã§ãã£ãã2åæäžã§ã®åèçã¯å¯Ÿç
§çŸ€ã®66.7%ã«æ¯ã¹ããš10ããã³20ÎŒgæäžçŸ€ã§ã¯é«ã,ç¹ã«20ÎŒgæäžçŸ€ã§ã¯81.0%ãšé«ãå€ã§ãã£ããããã«,æ²»çæŽãæããç矀ãžã®æäžå¹æã«ã€ããŠã¯,察ç
§çŸ€ã®25.0%ã«æ¯ã¹åæäžçŸ€ã§57.1,36.4,52.9%ãšé«ãåèçãåŸãããã以äžã®æ瞟ãã,çã«ãããŠäººå·¥æ粟12æ¥ç®ã®ãã»ã¬ãªã³æäžã¯,åèçãåäžãããããšã瀺åãã,ãã»ã¬ãªã³10ÎŒgæäžéã§ã®å¹æã20ÎŒgæäžãšåçãããã¯ãã以äžã§ãã£ããç¹ã«ãªããŒãããªãŒããŒãªã©ãå«ãåèãã«ããçã«å¯Ÿãããã»ã¬ãªã³æäžå¹æãé«ãããšã瀺åããããGonadotropin-releasing hormone (GnRH ; buserelin) was administered 12 days after artificial insemination to 307 head of cattle bred by a stock-raising farmer in a public pasture and here at the Fuji Zootechnical station. Cattle were given doses of 6, 10 and 20ÎŒg respectively and pregnancy rates were compared to and examined with those of a non-treated control population. For the control population, the pregnancy rate was 60.0%, while for the treated group which was administered 10 and 20ÎŒg of buserelin, a high pregnancy rate of 72.3 and 68.6% respectively, resulted. After the group was divided into two, a short division (less than 120 days) group and long division (120 days or more) group, the rates were examined. For the short division group, the 6, 10 and 20ÎŒg groups showed pregnancy rates higher than the 59.0% in the control population. For the long division group, the control population rate of 63.2% was low in comparison to that of the 6ÎŒg group. However, it was high in the 10 and 20ÎŒg groups. Moreover, in comparison with the 10 and 20ÎŒg group, the conception rate for one-time administration also tended to be high in the control population at 59.2%. We examined administration frequency by dividing the group into one-time and two-time groups and found the rate to be high in the 10ÎŒg group, at 73.3%. In the 10 and 20ÎŒg groups, the pregnancy rate after two-time administration was high when compared with the 66.7% in the control population, and especially high in the 20ÎŒg group at 81.0%. In addition, a high pregnancy rate was obtained due to effects of administration in cattle with medical treatment history at 57.1, 36.4 and 52.9% in comparison to the 25.0% in the control population. The above results indicate that the pregnancy rate improved with buserelin administration 12 days after artificial insemination in cattle and that the effect of a 10ÎŒg buserelin dose was equivalent to a 20ÎŒg dose. Of special note is the effectiveness of buserelin administration on cattle including repeat breeders etc., which had pregnancy difficulties
ãŠã· ã ãªã±ã« ãžã³ã³ãŠ ãžã¥ã»ã€ ã ã»ã€ã»ã³ ã·ã²ã ãã«ã¢ã³ ããŠã·ã¥ã ãã«ã¢ã³ ããŠãš 㬠ãžã¥ã¿ã€ ã ãªãšãã¹ ãšã€ãã§ãŠ
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ºåºæ¿ãã«ã¢ã³æŸåºãã«ã¢ã³(GnRH,ãã»ã¬ãªã³)ã6,10ããã³20ÎŒgãæäžã,ç¡åŠçœ®å¯Ÿç
§çŸ€ã®ãããšåèçã«ã€ããŠæ¯èŒããããŸã,æ¬å€ã®æäžå¹æãå€å®ããããã«è¡æŒ¿äžãããžã§ã¹ããã³æ¿åºŠã枬å®ã,é»äœæ©èœãžã®åœ±é¿ã«ã€ããŠãæ€èšããããã®çµæ,ãã»ã¬ãªã³6,10ããã³20ÎŒgæäžã®ãããã®çŸ€ã«ãããŠãåèçã¯å¯Ÿç
§çŸ€ã®ããããã7.6ïœ9.2%ãšé«ãæ瞟ãåŸãããã次ãã§,空èæéã®çãåº(120æ¥æªæº)ãšé·ãåº(120æ¥ä»¥äž)ã«åããŠæ€èšãããšãã,空èæéã®é·çã«é¢ä¿ãªãåèçã¯æäžçŸ€ã®æ¹ã察ç
§çŸ€ãããé«ãåŸåã瀺ãããããã«,æäžåæ°ã1åãš2åã«åãããšãã,1åç®ã®æäžã§ã®åèçã¯,åæäžçŸ€ãšå¯Ÿç
§çŸ€ã®éã«ææå·®ã¯ãªãã£ãã,2åç®ã®æäžã§ã¯æäžçŸ€ã®åèçã¯å¯Ÿç
§çŸ€ããã16.4ïœ47.6%æ¹åãã,ç¹ã«10ÎŒgæäžçŸ€ã§ã®åèçã¯85.7%ã§å¯Ÿç
§çŸ€ããã³6ÎŒgæäžçŸ€ãããææ(P<0.05)ã«é«ãããšãèªãããããåŸã£ãŠ,åèãã«ããçã«å¯Ÿãããã»ã¬ãªã³æäžã®å¹æãé¡èã«çŸãããGonadotropin-releasing hormone (GnRH, buserelin) was administered to 418 head of cattle bred by a general stock- raising farmer at a public pasture and here at the Fuji Zootechnical station, simultaneously during artificial insemination at doses of 6, 10 and 20ÎŒg respectively. Pregnancy rates for this group and for a non-treated control population were then compared. Blood progesterone concentration was also measured in order to determine the effect of this treatment. The effect on the luteal function was also examined. For buserelin, results were of 6ÎŒg and 10ÎŒg doses. Pregnancy rates for both groups with administered doses of 20ÎŒg resulted in a high 7.6ïœ9.2%. The pregnancy rate for the buserelin-administered group was higher than that of the control population regardless of the merits and demerits during the non-pregnant period when they were examined. Subsequently, it was divided into the short division (less than 120) of a non-pregnant condition period, and the long division (120 or more) and investigated. In addition, there was no significant effect on the pregnancy rate following the first administration in each treated group and control population, when the administration frequency was divided into 1 and 2 times. During the second administration, the pregnancy rate of the treated group improved to 16.4ïœ47.6% more than the control population and at a specially notable 85.7% pregnancy rate in the 10ÎŒg-dose administered group control population. It might be significantly higher (P<0.05) than the 6ÎŒg-dose administered group. Therefore, it is difficult to accurately demonstrate the effect of buserelin treatment on cattle pregnancy rates
ã®ã ãžã㪠ãªã¹ ã ã¿ã€ã¹ã« ã¡ã€ ã ã ãªã±ã« ãã³ã㯠ã¹ãã¬ã¹ 㬠ã±ãã·ã§ãŠ ã³ã«ãã³ã¹ããã³ ããŠã ã ãªãšãã¹ ãšã€ãã§ãŠ
ææã«ãããç·çžã¹ãã¬ã¹ãè¡æŒ¿ã³ã«ãã³ã¹ããã³æ¿åºŠã«åãŒã圱é¿ãæ€èšãããã,å²éå°é¶éãçšããŠ,6 : 00,12 : 00,17 : 00(14L : 10D,5 : 00ç¹ç¯)ã«ãããè¡æŒ¿ã³ã«ãã³ã¹ããã³æ¿åºŠãšã¹ãã¬ã¹ã«å¯Ÿããåå¿æ§ãæ¯èŒãããè¡æŒ¿ã³ã«ãã³ã¹ããã³æ¿åºŠã¯,6 : 00(2.62±0.24ng/ml)ã®å€ãæãé«ã,12 : 00(1.47±0.40ng/ml),17 : 00(1.02±0.19ng/ml)ãšäœãå€ã瀺ãã(P<0.05)ãã©ã®æå»ã«ãããŠã,è¡æŒ¿ã³ã«ãã³ã¹ããã³æ¿åºŠã¯ç·çžã¹ãã¬ã¹ã«ããäžæããã6 : 00ã«ãããŠ,è¡æŒ¿ã³ã«ãã³ã¹ããã³æ¿åºŠã¯ã¹ãã¬ã¹è² è·åŸ0ïœ60åãŸã§ä»ã®æå»ã®ãã®ããé«æ¿åºŠã§æšç§»ãããã¹ãã¬ã¹ã«å¯Ÿããåå¿æ§ã¯,17 : 00ãæãé«ã,12 : 00,6 : 00ã®é ã§äœããªãé¢ä¿ã瀺ããã以äžã®çµæãã,å²éå°é¶éã®è¡æŒ¿ã³ã«ãã³ã¹ããã³æ¿åºŠã¯ææéå§åŸã«é«ãããš,ææéå§åŸã¯,ã¹ãã¬ã¹è² è·åŸãæ¿åºŠãé«ãç¶æãç¶ããããš,ãŸã,ã¹ãã¬ã¹ã®åå¿æ§ã¯ææéå§åã«é«ããªãããšãæãããšãªã£ããIn order to consider the effect of stress on plasma concentration of corticosterone during light periods, we compared plasma concentration of corticosterone with reactivity to stress at 6 : 00, 12 : 00, and 17 : 00 (14L : 10D, light on at 5 : 00) in Gifujidori roosters. Plasma concentration of corticosterone was the highest value at 6 : 00 (2.62±0.24ng/ml), and became low in order of 12 : 00 (1.47±0.40ng/ml) and 17 : 00 (1.02±0.19ng/ml) (P<0.05). At all times, plasma concentration of corticosterone increased after immobilization stress. At 6 : 00, plasma concentration of corticosterone showed a higher level than that of the other time 0-60 minutes after restraint. Reactivity to stress was the highest at 17 : 00, and became low in order of 12 : 00 and 6 : 00. Therefore, in Gifujidori roosters, it became clear that plasma concentration of corticosterone was high after the onset of light period, it also continued to be high after stress, and reactivity to stress became high before the onset of dark period when plasma concentration of corticosterone became low
ãã€ã¯ããã¬ãŒã 㪠ã¢ãã€ã¿ ãªã¹ ã·ãã€ã® ã±ãã·ã§ãŠ ãã¥ãŠ ãã¹ãã¹ããã³ ã ã³ãŠãœ ã¡ã³ãšã ãœã¯ã〠ã㊠ã ã±ã³ããŠ
Testosterone-3(E)-carboxymethyloxime-BSAãæåãšããŠäœæããæäœ,ãªãã³ã«é
µçŽ æšèãã«ã¢ã³ãšããŠTestosterone-3(E)-carboxymethyloxime-peroxidaseã䜿çšã,éã·ãã€ã®ã«ãããè¡æŒ¿äžãã¹ãã¹ããã³æ¿åºŠã®é
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ã¯,350,000åã«åžéããŠã䜿çšå¯èœãªé«ãå䟡ãæããŠãããè¡æŒ¿ã«10ïœ100pgã®ãã¹ãã¹ããã³ãæ·»å ããæ·»å ååè©Šéšã§ã¯,ååçãå¹³å102.8±2.8%ãšãªã£ãã枬å®å
å€åä¿æ°(N=6)ã¯éã·ãã€ã®é žéèè¡æŒ¿ã§5.08%,粟玢éèè¡æŒ¿ã§ã¯7.32%,枬å®éå€åä¿æ°(N=6)ã¯,é žéèè¡æŒ¿ã§5.74%,粟玢éèè¡æŒ¿ã§6.13%ã§ãã£ãã以äžã®çµæãã,æ¬æ¹æ³ã«ãã£ãŠéã·ãã€ã®è¡æŒ¿äžãã¹ãã¹ããã³ã®æž¬å®ãå¯èœãšãªã£ããEnzymeimmunoassay (EIA) of testosterone was examined in which an individual antibody, and testosterone-peroxidase-conjugate were used for that, was measured in male Shiba-goat plasma. An anti-testosterone-3(E)-carboxymethyloxime-BSA antibody was used as an anti-serum, and testosterone-3(E)-carboxymethyloxime-peroxidase was used as a steroid-enzyme conjugate. The anti-serum was diluted by using 2nd antibody method which could get high measurement sensitivity of 350,000 times. Recovery rates of testosterone for each concentration with the addition of 10ïœ100pg to Shiba-goat plasma were 102.8±2.8% of the averages. Inter-assay coefficient of variation (C.V.) for testosterone level from jugular and testicular vein blood plasma samples were 5.08% and 7.32% respectively, as for intra-assay, they became 5.74% and 6.13%. These results suggest that the EIA method is extremely suitable to measure testosterone concentration in blood plasma of the male Shiba-goat
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µçŽ æšèãã«ã¢ã³ã«ã¯5α-DHT-11α-Succinate-peroxidase(5α-DHT-HRP)ãçšãããæè¡æž
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ã«ã¯ãã¹ãã¹ããã³ã30%亀ååå¿ãããã,Bond Elut CN-Uãçšããã«ã©ã ã¯ãããã°ã©ãã£ãŒã«ãã粟補ãè¡ã,被æ€è¡æŒ¿äžã®5α-DHTãšãã¹ãã¹ããã³ãåé¢ã»æž¬å®ããããã®çµæ,é
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žãšãã« : ãã³ãŒã³=2 : 98ã®å±é液ãæµãããšãã,1.00-4.25mlã®ç¯å²ã«5α-DHTã溶åºããããæ·»å ååè©Šéšã«ãããŠæ·»å é0.1-1.0pgã®åæ¿åºŠã§ã®ååçã¯,å¹³å100.45%±2.13ãšãªã£ããåçŸæ§è©Šéšã«ãããé žéèè¡ããã³ç²Ÿå·£éèè¡ã®æž¬å®å
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6.38%ããã³5.94%ãšãªã,枬å®éå€åä¿æ°ã¯8.36%ãªãã³ã«11.53%ãšãªã£ãã以äžã®çµæãã,éã·ãã€ã®ã®è¡æŒ¿äž5α-DHTæ¿åºŠã,æ¬æ³ãçšããŠæž¬å®ããããšãå¯èœã§ããããšãæããã«ãããEnzymeimmunoassay (EIA) for 5α-dihydrotestosterone (5α-DHT) in male Shiba-goat blood plasma was examined. The antiserum used 5α-DHT-11α-succinate-peroxidase (5α-DHT-HRP) for the enzyme labeling hormone in respect of 5α-DHT-11α-succinate-BSA. The use was possible for the antiserum at 100,000 times. The 5α-DHT in plasma was purified and separated from the testosterone by column chromatography using Bond Elut CN-U, since antiserum for 5α-DHT had cross reaction (30%) to the testosterone. The 5α-DHT was washed away by the development liquid of ethyl acetate: benzene=2 : 98 and was collected in the range between 1.00 and 4.25ml. Recovery rates of 5α-DHT each concentration of the addition 0.1ïœ1ng to Shiba-goat plasma were 100.45%±2.13 of the averages. Inter-assay coefficient of variation (C.V.) became respectively 6.38% and 5.94% in jugular and testicular vein blood,while for intra-assay, they became 8.36% and 11.53%. It was possible to analyse 5α-DHT concentration in blood plasma of the male Shiba-goat from the above result using this method
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就巣æ§ãæããŠããå²éå°é¶ã«çµ¶é£åŠçãæœã,äœéåã³è¡äžãšã¹ãã©ãžãªãŒã«æ¿åºŠã枬å®ã,ãããã®å€åãšæ¬¡æç£åµæ¥æ°ãšã®é¢ä¿ã«ã€ããŠæ€èšãããäŸè©Šé¶ã¯æ±åµéå§3æ¥ç®ãã7æ¥é絶é£åŠçãå®æœãããåŠçéå§ããæ¯æ¥12 : 00ã«æ¡è¡åã³äœé枬å®ã15æ¥éè¡ã£ãããã®çµæ,4äŸäž1äŸãåŠçåŸãæ±åµè¡åãç¶ç¶ãããæ±åµè¡åãç¶ç¶ããåäœã®äœéã¯åŠçéå§ããæžå°ãç¶ã,ãšã¹ãã©ãžãªãŒã«æ¿åºŠã¯äœãå€ã§æšç§»ããããŸã,æ±åµè¡åãäžæ¢ããåäœã®äœéã¯åŠçæéäžã¯æžå°ããã,åŠççµäºåŸæ¥æ¿ãªå¢å ã瀺ããããšã¹ãã©ãžãªãŒã«æ¿åºŠã¯åŠçäžã¯äœãå€ã§æšç§»ããŠããã,æ±åµè¡åãäžæ¢ããåäœã¯åŠççµäºåŸæ¥æ¿ã«äžæããããããã®é¶ã¯çµ¶é£åŠçéå§ãã21.0±0.7æ¥ã§æ¬¡æç£åµãéå§ãããFasting was performed on incubating Gifujidori hens. The body weight and plasma concentration of estradiol were measured during the experimental period. The changes and relationships with the onset of the next egg laying were examined. Experimental hens underwent fasting for seven days from the 3rd day of the onset of incubation. Blood collection and weight measurement were carried out daily at 12 : 00, for 15 days from the 3rd day of the onset of incubation. Measurement of the estradiol concentration in the blood was performed using the time decomposition fluorescence immunity measuring method. Only one in five hens continued incubation behavior after that result. The body weight of the individual which continued incubation behavior continued to decrease from fasting, and the estradiol concentration in the plasma decreased to a low value. Moreover, although the weight of the individual which stopped incubation behavior decreased during fasting, it rapidly increased after the fasting ended. Although estradiol concentration decreased to a low value during fasting, it rose rapidly after the end of fasting. The hens started laying from the onset of fasting in 21.0±0.7 days
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ç«ã®ã¿ã«ããéæåµèªèµ·åŠçœ®ã®æåäŸãšããŠ,家çã«ãããåã®å ±åäŸã§ãããInhibin immunization increases the ovulation rate in cows. In this study we investigated the effect of active immunization of Japanese Black cows against the recombinant ovine inhibin α subunit on ovarian activity. In addition we examined whether inhibin immunization could be applied to superovulation and twinning in Japanese Black cows. Cows (n=8) were injected with 2ml of inhibin vaccine as primary injection at estrous day, followed by a booster injection (2ml) 4 weeks later. The control cows (n=5) received adjuvant only. Ovaries were examined by rectal palpation and using ultrasonography during 2 to 5 months after primary. Superovulation and twinning were attempted on 2 and 4 of 23 estrous cycles in immunized cows, respectively. The cows were run with artificial insemination at the day of estrus for both trials, and embryos were recovered for superovulation at 8 days after artificial insemination. The number of estrous follicles (10mmâŠ) and corpora lutea (7.0±1.9, 3.1±1.2, 23 cycles) of the immunized cows were higher than those of control cows (1.1±2.2, 1.1±1.4, 16 cycles). Multiple follicular development and ovulation were observed during successive estrous cycles in the immunized cows. In the superovulation trial, nine and 4 transferable embryos were recovered from each cow. The transferred embryos resulted in the birth of three calves. One of 4 cows of the twinning trial gave birth to twin calves. In conclusion, active immunization against the recombinant ovine inhibin α subunit induces successive multiple development of estrous follicles and multiple ovulation in Japanese Black cows. These experiments also show that inhibin immunization can be applied for superovulation in Japanese Black cows. In addition one twinning was induced in immunized cows. The present study is the first reported application of inhibin immunization resulting in successful superovulation in domestic animals
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åšæãççž®ãããããšãå¯èœã§ãã£ããWe previously reported that active immunization against the recombinant ovine inhibin α subunit can enhance follicular development and increase the ovulation rate during successive estrous cycles in Japanese Black cows. However some problems have remained in efficiently inducing the ovarian reaction. The aims of this study were to examine the effects of repeated booster injections and prostaglandin F2α administration on ovarian activity and estrous cycle length in immunized Japanese Black cows in order to make the ovarian response more efficient. In four cows immunized against the recombinant ovine inhibin α subunit, two or three booster injections were given at various intervals over a period of 7 months. In addition, during the last 2 months following the final booster injection, immunized cows were treated three times with prostaglandin F2α. Five control cows received adjuvant only. Ovaries were examined by rectal palpation and by ultrasonography, and days of estrus were recorded. The second booster injection enhanced follicular development (17.2±3.6 vs. 3.0±2.8 P<0.01) and multiple ovulations (9.6±2.9 vs. 0.8±2.2 ; P<0.05) in comparison with the first booster injection. In addition, the third booster injection induced the same ovarian responses as the second booster injection. The length of the estrous cycle in immunized cows was significantly longer than that of the control cows (43.0±5.2 days vs. 23.1±4.7 ; P<0.01), due to multiple formation of CL. The prostaglandin F2α administration in immunized cows reduced estrous cycle length significantly (P<0.01) without any effect on ovarian response. In conclusion, repeated booster injections enhance the repeated ovarian responses in inhibin-immunized Japanese Black cows. In addition, utilization of prostaglandin F2α enabled the shortening of the length of the estrous cycle without any effect on ovarian response in inhibin-immunized cows
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飌è²åç©ã®ããã¯ããããè¿äŒŒããå€ã瀺ãããThe present study was conducted to study the shape of hair from genus Llama. The hair of llama was collected from animals fed in Japan and Peru. The hair of alpaca was collected from animals fed in Japan. The hair of guanaco and vicuna were supplied from the co-research member in University of National Agriculture La Molina. The hair of sheep (suffork) was collected from animals fed in our laboratory. Expansibility, number of crimps, thickness, and dimensions and form of cuticle were determined by optical and electron microscopic observation. The expansibility of hair from genus Llama was lower than that from sheep, conversely, the number of crimps in hair from genus Llama was higher than that from sheep. This suggested that the flexibility of hair from genus Llama is lower than that from sheep. The results of thickness showed that the crude hair from llama was 2-3 times higher than the other animals\u27 usual hair. And the thickness of hair from vicuna was half the size of the other animals\u27 usual hair. In the results of electron microscopic observation, the form of cuticle was classified into two groups, one of which showed the wide width type (llama, alpaca, guanaco and sheep) and vicuna showed the long length type. The hair of vicuna showed the lowest value of cuticle dimensions and the other animals\u27 hair showed the nearly the same values without it being fed in Peru
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ã®äº€éçš®1é ãšãã®ã¢ã«ãã«ãžã®æ»ã亀éçš®1é ã®èš76é ã®è¡æ¶²ãçšããŠ,19座äœã®è¡æ¶²ã¿ã³ãã¯è³ªã»é
µçŽ åãé»æ°æ³³ååŠçã«è§£æã,以äžã«ç€ºãæ瞟ãåŸãã1)è¡æŒ¿ã¿ã³ãã¯è³ª : Albumin, Haptoglobin,è¡æŒ¿é
µçŽ : Alanine aminotransferase, Aspartate aminotransferase, γ-Glutamyltranspeptidase,èµ€è¡çã¿ã³ãã¯è³ª : Haemoglobin,ãªãã³ã«èµ€è¡çé
µçŽ : Acid phosphatase, Catarase, Glucose-6-phosphate dehydrogenase, Phosphoglucomutase, Phosphohexose isomeraseã®èš11座äœã§ã¯å€åã¯èªããããªãã£ãã2)è¡æŒ¿ã¿ã³ãã¯è³ª4åº§äœ : Post-albumin(Po),Gc-protein(Gc),Transferrin(Tf)ããã³Î³-globurin field protein(γG),è¡æŒ¿é
µçŽ 3åº§äœ : Amylase(Amy),Creatine kinase(CK)ããã³Leucine aminopeptidase(LAP),ãªãã³ã«èµ€è¡çé
µçŽ 1åº§äœ : EsteraseD(EsD)ã®èš8座äœã«å€åãèªãããããããã8座äœã®ãã¡Tfã§ã¯6å,Poããã³Gcã§ã¯4å,γ-G, Amyããã³EsDã§ã¯3å,LAPããã³CKã§ã¯2åãèªãããããã©ãããã³ã¢ã«ãã«ã«ãããããã8座äœã®ç·åçãªç¶æš©åŠå®çã¯,0.931ããã³0.867ã§ãã£ãã3)ã©ããšã¢ã«ãã«ãšã®éã§è¡æ¶²ã¿ã³ãã¯è³ªã»é
µçŽ åãæ¯èŒãããšãã,Gc, Amyããã³EsDã«ãããŠçš®éå·®ãèªãããããããªãã¡,Gcããã³Amyã§ã¯äž¡çš®ã§å
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çš®ç¹æã®æå床ã瀺ããã³ããååšã,ãŸãEsDã§ã¯äž¡çš®éã§ãã³ãã®æå床ãç°ãªã£ãŠããã4)ã©ããšã¢ã«ãã«ã®äº€éçš®,ãªãã³ã«ã¢ã«ãã«ãžã®æ»ã亀éçš®ã®è¡æ¶²ã¿ã³ãã¯è³ªã»é
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±éã§ãã£ãã5)CKããã³EsDãé€ã,17座äœã®éºäŒåé »åºŠã«åºã¥ããŠç®åºããã©ããšã¢ã«ãã«ãšã®éã®éºäŒçè·é¢ã¯0.035ã§ãã£ãã以äžã®æ瞟ãã,è¡æ¶²ã¿ã³ãã¯è³ªã»é
µçŽ åã®è§£æã¯ã©ãããã³ã¢ã«ãã«ã®éå£ã®éºäŒåæ§æãæšå®ããäžã§æåãªææšãšãªãããšãæ確ãšãªã£ããäžæ¹,ã©ããšã¢ã«ãã«ãéºäŒçã«æ¥µããŠè¿çžãªé¢ä¿ã«ããããšãè£ä»ãããã®ãšå€æããããBlood samples of llamas and alpacas were classified by using electrophoretic procedures in the polymorphism at 19 loci. Electrophoretic variation was found for 8 loci, namely plasma proteins : post albumin (Po), Gc protein (Gc) and transferrin (Tf) and γ-globurin zone protein (γG), for plasma enzymes : amylase (Amy), creatine kinase (CK) and leucine aminopeptidase (LAP), and for red cell enzyme : esteraseD (EsD). Synthetic probabilityes of paternity exclusion about the 8 loci for llamas and alpacas were 0.931 and 0.867, respectively. No variants were found for plasma proteins : albumin and haptoglobin, for plasma enzymes : alanine aminotransferase, aspartate aminotransferase and γ-glutamyltranspeptidase, for red cell haemoglobin, and for red cell enzymes : acid phosphatase, catarase, glucose-6-phosphate dehydrogenase, phosphoglucomutase and phosphohexose isomerase. Nei\u27s genetic distance between llamas and alpacas on the 17 loci (except CK and EsD) was 0.035. Preliminary estimate of the genetic distance measure may suggest that llamas and alpacas are more likely related as subspecies than as separate species