Conservación de orquídeas nativas de Entre Ríos utilizando técnicas de cultivo de tejidos “in vitro”

Este proyecto de investigación se enmarca dentro de la investigación básica y aplicada con transferencia.El preocupante avance de la agriculturización sobre áreas de montes nativos y selvas ribereñasamenaza la biodiversidad. En tal sentido nos preocupa la pérdida de especies nativas de interés desdeel punto de vista ecológico, agronómico u ornamental. El proyecto se centra en el rescate y propagaciónde especies nativas de orquídeas de la zona del litoral, en particular las que crecen en la diversidad dehábitats palustres y selvas ribereñas de los arroyos de Entre Ríos, contribuyendo a la preservación delas especies ante el avance de las actividades antrópicas en los ecosistemas.El objetivo es propagar por técnicas de cultivo “in vitro” especies terrestres y epífitas de orquídeasnativas de la Provincia de Entre Ríos, hasta la etapa de aclimatación de plantas en macetas y/o palos,confeccionando los respectivos protocolos de micropropagación y aclimatación para las especies evaluadas.A partir de semillas, se buscará eficientizar la producción de protocormos y el establecimiento deplantitas “in vitro”, las que serán donadoras de explantos para inducir organogénesis directa o indirecta,con diferentes combinaciones hormonales.La transferencia, a través de cursos, charlas técnicas y cartillas, ofrecidas a viveristas, asociacionesde orquidiófilos y jardines botánicos, contribuirá a la toma de conciencia para no adquirir, ni venderrecursos nativos, en su estado original y propiciar la compra de estas especies obtenidas por técnicasde cultivo de tejidos

Insights from an Integrated View of the Biology of Apple Snails (Caenogastropoda: Ampullariidae)

Submitted by sandra infurna ([email protected]) on 2016-02-16T12:59:35Z No. of bitstreams: 1 silvana_thiengo_etal_IOC_2015.pdf: 1030588 bytes, checksum: 1feaf6021ccd94c9bf314dbc7b49ccc8 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-02-16T13:49:31Z (GMT) No. of bitstreams: 1 silvana_thiengo_etal_IOC_2015.pdf: 1030588 bytes, checksum: 1feaf6021ccd94c9bf314dbc7b49ccc8 (MD5)Made available in DSpace on 2016-02-16T13:49:31Z (GMT). No. of bitstreams: 1 silvana_thiengo_etal_IOC_2015.pdf: 1030588 bytes, checksum: 1feaf6021ccd94c9bf314dbc7b49ccc8 (MD5) Previous issue date: 2015Howard University. Department of Biology. Washington, DC, USA / University of Hawaii. Pacific Biosciences Research Center. Honolulu, Hawaii, USA /Smithsonian Institution. National Museum of Natural History. Washington, DC, USA.Southwestern University. Department of Biology. Georgetown, Texas, USA.Instituto de Fisiología (FCM-UNCuyo). Laboratorio de Fisiología (IHEM-CONICET). Mendoza, Argentina.University of West Florida. Department of Biology. Pensacola, Florida, USA.Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP, CONICET). La Plata, Argentina.Universidad Nacional del Sur-CONICET. Laboratorio de Ecología, INBIOSUR. Bahia Blanca, Argentina.Hong Kong Baptist University. Department of Biology. Kowloon, Hong Kong.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malacologia. Rio de Janeiro, RJ, Brasil.Instituto de Fisiología (FCM-UNCuyo). Laboratorio de Fisiología (IHEM-CONICET). Mendoza, Argentina / Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Área de Biologia. Mendoza, Argentina.NARO Kyushu Okinawa Agricultural Research Center. Kumamoto, Japan.Nara Women’s University. Faculty of Science. Kitauoya-nishi, Nara, Japan.Universidad Nacional del Sur-CONICET. Laboratorio de Ecología, INBIOSUR. Bahia Blanca, Argentina.Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP, CONICET). La Plata, Argentina.Instituto de Fisiología (FCM-UNCuyo). Laboratorio de Fisiología (IHEM-CONICET). Mendoza, Argentina / Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Área de Biologia. Mendoza, Argentina.Instituto de Fisiología (FCM-UNCuyo). Laboratorio de Fisiología (IHEM-CONICET). Mendoza, Argentina / Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Área de Biologia. Mendoza, Argentina.Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP, CONICET). La Plata, Argentina / Comisión de Investigaciones Científicas (CIC). La Plata, Argentina.Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP, CONICET). La Plata, Argentina.Instituto de Fisiología (FCM-UNCuyo). Laboratorio de Fisiología (IHEM-CONICET). Mendoza, Argentina / Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Área de Biologia. Mendoza, Argentina.Instituto de Fisiología (FCM-UNCuyo). Laboratorio de Fisiología (IHEM-CONICET). Mendoza, Argentina / Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Área de Biologia. Mendoza, Argentina.Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP, CONICET). La Plata, Argentina.Instituto de Fisiología (FCM-UNCuyo). Laboratorio de Fisiología (IHEM-CONICET). Mendoza, Argentina / Universidad Nacional de Cuyo. Facultad de Ciencias Exactas y Naturales. Área de Biologia. Mendoza, Argentina.University of Hawaii. Pacific Biosciences Research Center. Honolulu, Hawaii, USA / NARO Kyushu Okinawa Agricultural Research Center. Koshi, Kumamoto, Japan.Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP, CONICET). La Plata, Argentina.Instituto de Fisiología (FCM-UNCuyo). Laboratorio de Fisiología (IHEM-CONICET). Mendoza, Argentina.Universidad Nacional del Sur-CONICET. Laboratorio de Ecología, INBIOSUR. Bahia Blanca, Argentina.Universidad Nacional del Sur-CONICET. Laboratorio de Ecología, INBIOSUR. Bahia Blanca, Argentina.Smithsonian Institution. National Museum of Natural History. Washington, DC, USA..Hong Kong Baptist University. Department of Biology. Kowloon, Hong Kong.Universidad Nacional del Sur-CONICET. Laboratorio de Ecología, INBIOSUR. Bahia Blanca, Argentina.Universidad Nacional del Sur-CONICET. Laboratorio de Ecología, INBIOSUR. Bahia Blanca, Argentina.Florida Institute of Technology. Biological Sciences Department. Melbourne, Florida, USA.The Pomacea Project, Inc., Pensacola, Florida, USA.University of Hawaii. Pacific Biosciences Research Center. Honolulu, Hawaii, USA.Apple snails (Ampullariidae) are among the largest and most ecologically important freshwater snails. The introduction of multiple species has reinvigorated the field and spurred a burgeoning body of research since the early 1990s, particularly regarding two species introduced to Asian wetlands and elsewhere, where they have become serious agricultural pests. This review places these recent advances in the context of previous work, across diverse fields ranging from phylogenetics and biogeography through ecology and developmental biology, and the more applied areas of environmental health and human disease. The review does not deal with the role of ampullariids as pests, nor their control and management, as this has been substantially reviewed elsewhere. Despite this large and diverse body of research, significant gaps in knowledge of these important snails remain, particularly in a comparative framework. The great majority of the work to date concerns a single species, Pomacea canaliculata, which we see as having the potential to become a model organism in a wide range of fields. However, additional comparative data are essential for understanding this diverse and potentially informative group. With the rapid advances in genomic technologies, many questions, seemingly intractable two decades ago, can be addressed, and ampullariids will provide valuable insights to our understanding across diverse fields in integrative biology

Rare predicted loss-of-function variants of type I IFN immunity genes are associated with life-threatening COVID-19

BackgroundWe previously reported that impaired type I IFN activity, due to inborn errors of TLR3- and TLR7-dependent type I interferon (IFN) immunity or to autoantibodies against type I IFN, account for 15-20% of cases of life-threatening COVID-19 in unvaccinated patients. Therefore, the determinants of life-threatening COVID-19 remain to be identified in similar to 80% of cases.MethodsWe report here a genome-wide rare variant burden association analysis in 3269 unvaccinated patients with life-threatening COVID-19, and 1373 unvaccinated SARS-CoV-2-infected individuals without pneumonia. Among the 928 patients tested for autoantibodies against type I IFN, a quarter (234) were positive and were excluded.ResultsNo gene reached genome-wide significance. Under a recessive model, the most significant gene with at-risk variants was TLR7, with an OR of 27.68 (95%CI 1.5-528.7, P=1.1x10(-4)) for biochemically loss-of-function (bLOF) variants. We replicated the enrichment in rare predicted LOF (pLOF) variants at 13 influenza susceptibility loci involved in TLR3-dependent type I IFN immunity (OR=3.70[95%CI 1.3-8.2], P=2.1x10(-4)). This enrichment was further strengthened by (1) adding the recently reported TYK2 and TLR7 COVID-19 loci, particularly under a recessive model (OR=19.65[95%CI 2.1-2635.4], P=3.4x10(-3)), and (2) considering as pLOF branchpoint variants with potentially strong impacts on splicing among the 15 loci (OR=4.40[9%CI 2.3-8.4], P=7.7x10(-8)). Finally, the patients with pLOF/bLOF variants at these 15 loci were significantly younger (mean age [SD]=43.3 [20.3] years) than the other patients (56.0 [17.3] years; P=1.68x10(-5)).ConclusionsRare variants of TLR3- and TLR7-dependent type I IFN immunity genes can underlie life-threatening COVID-19, particularly with recessive inheritance, in patients under 60 years old