Professional competencies development of competitive bachelors in machine engineering

The significance of the problem being investigated is conditioned by the need of introduction of considerable amendments to academic discipline content with the objective of ensuring effective education process and professional competencies development level increase of bachelors in machine engineering necessary in their professional activities while performing employment functions. The purpose of the article involves structural-functional model development of forming bachelors’ professional competencies towards “Machine Engineering” qualifications in studies of qualified educational subjects, and also identifying a set of organizational-educational conditions of its successful implementation. The key method of the given problem analysis is meant to be model analysis which enables to study the matter point of this problem in great detail. The fundamental result of the article is a generated structural functional model of professional competencies forming, including information-packed, assignment, and evaluative units in its structure and being implemented with respect to organizational-educational conditions. The content of the article may prove helpful for faculty members of technical institutions for the purposes of high quality vocational training implementation which meets unit standard requirements of machine-building complex enterprises. © 2016 Dorozhkin et al

Determination of Coenzyme A and Acetyl-Coenzyme A in Biological samples Using HPLC with UV Detection

Coenzyme A (CoA) and acetyl-coenzyme A (acetyl-CoA) play essential roles in cell energy metabolism. Dysregulation of the biosynthesis and functioning of both compounds may contribute to various pathological conditions. We describe here a simple and sensitive HPLC-UV based method for simultaneous determination of CoA and acetyl-CoA in a variety of biological samples, including cells in culture, mouse cortex, and rat plasma, liver, kidney, and brain tissues. The limits of detection for CoA and acetyl-CoA are \u3e10-fold lower than those obtained by previously described HPLC procedures, with coefficients of variatio

Hydrodynamic heat generator for the district heating network

Are considered problems of creation of Autonomous sources of heat supply for remote consumers, who are deprived of the main heating systems. The author proposes the creation of a hydrodynamic heat generator on the basis of commercially available components and parts, which is able to solve the problem of heat supply for remote consumers.В работе рассматриваются проблемы создания автономных источников теплоснабжения для удаленных потребителей, лишенных магистральных теплосетей. Автор предлагает создание гидродинамического теплогенератора на базе серийно выпускаемых узлов и деталей, который способен решить проблему теплоснабжения для удаленных потребителей

Hydrodynamic heat generator for the district heating network

Are considered problems of creation of Autonomous sources of heat supply for remote consumers, who are deprived of the main heating systems. The author proposes the creation of a hydrodynamic heat generator on the basis of commercially available components and parts, which is able to solve the problem of heat supply for remote consumers.В работе рассматриваются проблемы создания автономных источников теплоснабжения для удаленных потребителей, лишенных магистральных теплосетей. Автор предлагает создание гидродинамического теплогенератора на базе серийно выпускаемых узлов и деталей, который способен решить проблему теплоснабжения для удаленных потребителей

Reactor for the aerobic fermentation of biomass

Considers the problem of useful heat obtained from the process of fermentation of biomass. The authors proposed to use the reactor for the aerobic fermentation of biomass.Рассматриваются проблемы полезного использования теплоты, получаемой с процесса сбраживания биомассы. Авторами статьи предлагается использовать реактор для аэробной ферментации биомассы

Antitumor activity of the novel pyridine derivative

The study aim was to explore a toxicological property and antitumor action of the novel pyridine derivative LHT-17-19 in cell culture and on experimental models of lung cancer in mic

Molecular diagnostics of primary immunodeficiencies in Sverdlovsk region

The article presents the results of the work performed by the laboratory of molecular diagnostics at the Medical Center “Health Care of Mother and Child” for the diagnosis of primary immunodeficiency in Sverdlovsk region over 5 years. The laboratory was organized in 2009 to verify the diagnosis of monogenic hereditary diseases included in the Neonatal Screening Program in the Russian Federation, e.g., phenylketonuria, cystic fibrosis, classical galactosemia. Over time, the range of diagnosed nosologies expanded, and since 2014, the laboratory has included in studies of a new group of disorders, i.e., congenital errors of immunity. Every year the Regional Registry of patients with primary immunodeficiencies (PIDs) replenished by 20 to 70 persons, thus comprising 15 to 43% of the entire Russian Registry for these conditions. As of 03/01/2020, the registry of patients with a clinical diagnosis of “primary immunodeficiency” consisted of 526 people, more than half of them (275) being children under 18 years of age. According to the expert calculations, the frequency of detected PID cases in the Sverdlovsk region is 1:10 480 inhabitants, which indicates not only high level of the existing clinical immunology service, but also the high expected frequency of PID in the region. Until 2014, verification of the “primary immunodeficiency” diagnosis in the patients from Sverdlovsk region was traditionally carried out in Moscow clinics (Dmitry Rogachev National Medical Research Center of Pediatric Hematology, Oncology and Immunology, Moscow Research Centre for Medical Genetics). Over 6 years of cooperation between regional immunological service with the medical genetic center, 47 children received molecular genetic confirmation of the diagnosis of congenital immunity errors at the laboratory of Regional Medical Center “Health Care of Mother and Child”. The authors present the data of Regional Registry of patients, classified into nosological forms of immune-dependent pathology and provide a detailed description of diagnostic procedures for the patients with various PIDs. A deletion of chromosome 22 (Di Giorgi syndrome) was found in 43 people, mutations in the Btk gene (X-linked agammaglobulinemia) were revealed in 7 patients and 6 members of their families, Nijmegen syndrome was confirmed in 1 child, a familial case of ADA-deficiency, difficult for diagnostics, was decided. The results of the study encourage the authors for further expansion of the spectrum of detectable disorders diagnosis, and give a hope that development of regional laboratories at this level may improve the diagnostic algorithm for PID diagnostic procedures in Russia, i.e., from prenatal and neonatal screening to the development of gene therapy for certain forms of immune-dependent disorders

Неонатальный скрининг на тяжелую комбинированную иммунную недостаточность в России: прекрасное дале ко или завтрашняя реальность?

Mass screening of newborns in Russia for five hereditary diseases does not meet the requirements of the world community for the neonatal screening program. Success in the development of laboratory diagnostic technologies and active introduction of achievements in genetics and molecular biology into medical practice allow for the revision of the list of nosologies included in the national neonatal screening program by replacing the disease or adding new nosologies. The article discusses the possibility of including genetic testing for severe combined immune deficiency in the Newborn Screening Program in Russia. The results from a retrospective study of markers of naive T- and B-lymphocytes (TREC and KREC) in the group of children with immuno-dependent pathology developed in the first year of life are discussed. © 2017 Voprosy Sovremennoi Pediatrii - Current Pediatrics. All rights reserved

Gestational diabetes in modern population of inhabitants of the megalopolis

For studying of gestational diabetes (GSD) in modern population of inhabitants of the megalopolis survey of 516 pregnant women at the age from 18 till 42 years chosen in the blind kogortny way from all the patients who have registered on pregnancy in the Verkh-lsetsky district of Yekaterinburg was conducted. To all women the peroralny glyukozo-tolerantnny test (PGTT) was carried out. 31 cases of GSD from them a rodorazresheniye on the basis of scientific research institute OMM of 12 patients are revealed. The conclusion is drawn on prevalence and сруктуре by GSD and about need of continuous skriningovy inspection of all registered pregnant women.Для изучения гестационного сахарного диабета (ГСД) в современной популяции жительниц мегаполиса было проведено обследование 516 беременных женщин в возрасте от 18 до 42 лет выбранных слепым когортным способом из всех вставших на учет по беременности пациенток в Верх-Исетском районе г. Екатеринбурга. Всем женщинам был проведен пероральный глюкозо-толерантнный тест (ПГТТ). Выявлен 31 случай ГСД из них родоразрешение на базе НИИ ОММ 12 пациенток. Сделан вывод о распространенности и структуре ГСД и о необходимости сплошного скринингового обследования всех вставших на учет беременных женщин

Variability of CATCH-22 symptome complex within the framework of 22q11.2 deletion syndrome

Chromosomal pathology is one of the most common causes of congenital malformations. The CATCH-22 symptom complex is most often associated with a microdeletion of chromosome 22, upon detection of which it is customary to diagnose DiGeorge syndrome, a known primary immunodeficiency or syndrome of innate errors of immunity. According to our data on the frequency of occurrence among all chromosomal abnormalities, DiGeorge’s syndrome takes second place in the Sverdlovsk region after Down’s syndrome, but its diagnosis is not simple due to varying severity of clinical manifestations, as well as different forms of the chromosome 22 defects. Along with several typical variants of 22q11 microdeletions, there duplications of critical regions are also reported, accompanied by immunodeficiency and other symptoms of CATCH-22. The effectiveness of diagnosing chromosomal abnormalities both in pre- and postnatal period largely depends on the grouping criteria of the patients with suspected chromosomal abnormalities, and on the methods used to identify hereditary pathology. In our study, we analyzed and compared the results of studies of 23 patients with various rearrangements of the 22q11.2 region, which were observed by a geneticist and clinical immunologist. The paper presents data on the polymorphism of phenotypes associated with rearrangements of the 22q11.2 region with an analysis of pathomorphological manifestations depending on the type of structural anomaly, i.e, del22q11.2, or dup22q11.2. The results of analysis demonstrate importance of different diagnostic options for laboratory studies of microdeletion and microduplication syndromes associated with immune-dependent pathology. We also compared the results of molecular genetic diagnostics and phenotypic manifestations in deletions and duplications of the 22q11.2 region. To identify the rearrangements of 22q11.2 region, two different methods were used – Prenatal BoBs and multiplex ligase-dependent probes’ amplification (MLPA). In particular, the both methods were used in the same patient to verify diagnosis, thus enabling to show differences in their efficiency. It was concluded that 22q11.2 deletion syndrome exhibits wide heterogeneity in phenotypic traits: neurological and immunological manifestations, anomalies in musculoskeletal development and internal organs, skull deformities and facial dysmorphia. Each clinical case was unique, requiring careful analysis of clinical manifestations. It is necessary to have a wide range of laboratory options for molecular genetic verification of the diagnosis