Small Unmanned Aircraft Systems Detect Turfgrass Drought

Kansas State University is evaluating the ability of using small unmanned aircraft systems (UAS) to detect drought stress in turfgrass. Their research indicates high resolution remote sensing with small UAS can detect drought stress before it is visible to the human eye. Preliminary measurements of a golf course revealed interesting differences in fairways, tees, and greens between summer and fall seasons. Additional research will be conducted in 2016

Harmful Algal Bloom Characterization at Ultra-High Spatial and Temporal Resolution Using Small Unmanned Aircraft Systems

Citation: Van der Merwe, D., & Price, K. P. (2015). Harmful Algal Bloom Characterization at Ultra-High Spatial and Temporal Resolution Using Small Unmanned Aircraft Systems. Toxins, 7(4), 1065-1078. doi:10.3390/toxins7041065Harmful algal blooms (HABs) degrade water quality and produce toxins. The spatial distribution of HAbs may change rapidly due to variations wind, water currents, and population dynamics. Risk assessments, based on traditional sampling methods, are hampered by the sparseness of water sample data points, and delays between sampling and the availability of results. There is a need for local risk assessment and risk management at the spatial and temporal resolution relevant to local human and animal interactions at specific sites and times. Small, unmanned aircraft systems can gather color-infrared reflectance data at appropriate spatial and temporal resolutions, with full control over data collection timing, and short intervals between data gathering and result availability. Data can be interpreted qualitatively, or by generating a blue normalized difference vegetation index (BNDVI) that is correlated with cyanobacterial biomass densities at the water surface, as estimated using a buoyant packed cell volume (BPCV). Correlations between BNDVI and BPCV follow a logarithmic model, with r(2)-values under field conditions from 0.77 to 0.87. These methods provide valuable information that is complimentary to risk assessment data derived from traditional risk assessment methods, and could help to improve risk management at the local level

Evaluating Small Unmanned Aerial Systems for Detecting Drought Stress on Turfgrass

This study was conducted to evaluate early detection ability of small unmanned aerial systems (sUAS) technology for drought stress on turfgrass. Certain reflectances collected by sUAS and a handheld device declined more in less irrigated treatments before drought stress was evident in visual quality rating (VQ) and percentage green cover (PGC). The near infrared (NIR) band and GreenBlue vegetation index performed the best consistently for drought stress prediction among the other vegetation indices (VI) or bands from sUAS. Results indicate using ultra-high resolution remote sensing with sUAS can detect drought stress as well as, if not better than, a handheld device before it is visible to the human eye, and may provide valuable evidence for irrigation management in turfgrass

Thermal Imaging Detects Early Drought Stress in Turfgrass Utilizing Small Unmanned Aircraft Systems

Plots of fairway-height creeping bentgrass were watered differently to create a gradient of drought stress from severe deficit irrigation to well-watered, under an automatic rainout shelter in Manhattan, KS. Canopy temperature (Tc) measured by a small unmanned aerial system (sUAS) predicted drought stress approximately 5 days or more before drought symptoms were evident in either turfgrass visual quality (VQ) or percentage green cover (PGC). The ability of Tc to predict drought stress was comparable to the best spectral parameters acquired by sUAS on companion flights [i.e., near infrared (NIR) and GreenBlue VI], and slightly better than with spectral data obtained from handheld sensors. Better drought-prediction ability combined with faster data collection using sUAS indicates significant potential for sUAS-based compared with ground-based drought stress monitoring methods

Spatial variation of epoxyscillirosidine concentrations in Moraea pallida (yellow tulp) in South Africa

Moraea pallida (yellow tulp) poisoning is economically the most important intoxication of livestock in South Africa. Poisoning varies according to locality, climatic conditions and growth stage of the plant. The primary objective of this study was to determine the concentration of the toxic principle, epoxyscillirosidine, in yellow tulp leaves and to ascertain the variability of epoxyscillirosidine concentrations within and between different locations. A secondary objective was to utilise Geographic Information Systems in an attempt to explain the variability in toxicity. Flowering yellow tulp plants were collected at 26 sampling points across 20 districts of South Africa. The leaves of five plants per sampling point were extracted and submitted for liquid chromatography/mass spectrometry analysis. A large variation in mean epoxyscillirosidine concentrations, ranging from 3.32 μg/g – 238.27 μg/g, occurred between different geographical regions. The epoxyscillirosidine concentrations also varied tremendously between individual plants (n= 5) collected at the same sampling point, with up to a 24 times difference between the lowest and highest concentration detected. No generalised correlation between epoxyscillirosidine concentrations and soil elemental concentrations could be established. However, samples obtained from the north-eastern part of the sampling region tended to have higher epoxyscillirosidine concentrations compared to samples obtained from the south-western part of the sampling region. Higher toxin concentrations in the northeast were associated with statistically significant higher soil concentrations of iron, bismuth, bromide, cadmium, chromium, rubidium, tellurium, thallium, titanium and zinc, whilst soil concentrations of strontium and soil pH, were significantly lower. This study corroborated the contention that epoxyscillirosidine concentration in yellow tulp fluctuates and may explain the variability in toxicity

Investigation of a Microcystis aeruginosa cyanobacterial freshwater harmful algal bloom associated with acute microcystin toxicosis in a dog

Microcystin poisoning was diagnosed in a dog exposed to a Microcystis aeruginosa dominated freshwater harmful algal bloom at Milford Lake, Kansas, which occurred during the summer of 2011. Lake water microcystin concentrations were determined at intervals during the summer, using competitive enzyme-linked immunosorbent assays, and indicated extremely high, localized microcystin concentrations of up to 126,000 ng/ml. Multiple extraction and analysis techniques were utilized in the determination of free and total microcystins in vomitus and liver samples from the poisoned dog. Vomitus and liver contained microcystins, as determined by enzyme-linked immunosorbent assays, and the presence of microcystin LR was confirmed in vomitus and liver samples using liquid chromatography coupled with tandem mass spectrometry. Major toxic effects in a dog presented for treatment on the day following exposure included fulminant liver failure and coagulopathy. The patient deteriorated rapidly in spite of aggressive treatment, and was euthanized. Postmortem lesions included diffuse, acute, massive hepatic necrosis and hemorrhage, and acute necrosis of the renal tubular epithelium. A diagnosis of microcystin poisoning was based on the demonstration of M. aeruginosa and microcystin-LR in the lake water, as well as in vomitus produced early in the course of the poisoning, the presence of microcystin-LR in liver tissue, and on a typical clinical course

Evaluations of land cover risk factors for canine leptospirosis: 94 cases (2002–2009)

Associations of land cover/land use variables and the presence of dogs in urban vs. rural address locations were evaluated retrospectively as potential risk factors for canine leptospirosis in Kansas and Nebraska using Geographic Information Systems (GIS). The sample included 94 dogs positive for leptospirosis predominantly based on a positive polymerase chain reaction test for leptospires in urine, isolation of leptospires on urine culture, a single reciprocal serum titer of 12,800 or greater, or a four-fold rise in reciprocal serum titers over a 2–4 weeks period; and 185 dogs negative for leptospirosis based on a negative polymerase chain reaction test and reciprocal serum titers less than 400. Land cover features from 2001 National Land Cover Dataset and 2001 Kansas Gap Analysis Program datasets around geocoded addresses of case/control locations were extracted using 2500 m buffers, and the presence of dogs’ address locations within urban vs. rural areas were estimated in GIS. Multivariate logistic models were used to determine the risk of different land cover variables and address locations to dogs. Medium intensity urban areas (OR = 1.805, 95% C.I. = 1.396, 2.334), urban areas in general (OR = 2.021, 95% C.I. = 1.360, 3.003), and having urban address locations (OR = 3.732, 95% C.I. = 1.935, 7.196 entire study region), were significant risk factors for canine leptospirosis. Dogs regardless of age, sex and breed that live in urban areas are at higher risk of leptospirosis and vaccination should be considered

Human Illnesses and Animal Deaths Associated with Freshwater Harmful Algal Blooms-Kansas

Citation: Trevino-Garrison, I., DeMent, J., Ahmed, F. S., Haines-Lieber, P., Langer, T., Menager, H., . . . Carney, E. (2015). Human Illnesses and Animal Deaths Associated with Freshwater Harmful Algal Blooms-Kansas. Toxins, 7(2), 353-366. doi:10.3390/toxins7020353Freshwater harmful algal bloom (FHAB) toxins can cause morbidity and mortality in both humans and animals, and the incidence of FHABs in the United States and Kansas has increased. In 2010, the Kansas Department of Health and Environment (KDHE) developed a FHAB policy and response plan. We describe the epidemiology of FHAB-associated morbidity and mortality in humans and animals in Kansas. Healthcare providers and veterinarians voluntarily reported FHAB-associated cases to KDHE. An investigation was initiated for each report to determine the source of exposure and to initiate public health mitigation actions. There were 38 water bodies with a confirmed FHAB in 2011. There were 34 reports of human and animal FHAB-associated health events in 2011, which included five dog deaths and hospitalization of two human case patients. Five confirmed human illnesses, two dog illnesses and five dog deaths were associated with one lake. Four human and seven dog cases were exposed to the lake after a public health alert was issued. Public health officials and FHAB partners must ensure continued awareness of the risks to the public, educate healthcare providers and veterinarians on FHAB-related health events and encourage timely reporting to public health authorities

Zinc, Copper, and Manganese Homeostasis and Potential Trace Metal Accumulation in Dairy Cows: Longitudinal Study from Late Lactation to Subsequent Mid-Lactation

BackgroundTrace metals are supplemented to cattle to prevent nutrient deficiencies. Levels supplemented to mitigate worst-case basal supply and availability scenarios can, however, result in trace metal intakes far above nutritional requirements of dairy cows with high feed intakes.ObjectivesWe evaluated Zn, Mn and Cu balance in dairy cows from late lactation through the subsequent mid-lactation, a period of 24 wk characterized by large changes in dry matter intake.MethodsTwelve Holstein dairy cows were housed in a tie-stall from 10 wk before to 16 wk after parturition, and fed one unique lactation diet when lactating, and a dry cow diet otherwise. After two weeks of adaptation to the facility and diet, Zn, Mn and Cu balances were determined at weekly intervals, by calculating the difference between total intakes and complete fecal, urinary and milk outputs, with the latter three fluxes quantified over a 48-h period. Repeated measures mixed models were used to evaluate the effects on trace mineral balances over time.ResultsThe Mn and Cu balances of cows were not significantly different from 0 mg·d-1 between 8 wk prepartum and calving (P ≥ 0.54), when dietary intake was the lowest of the period evaluated. However, when dietary intake was highest, between wk 6 and 16 postpartum, positive Mn and Cu balances were observed (80 and 20 mg·d-1, respectively, P ≤ 0.05). Cows were in positive Zn balance throughout the study except during the first 3 wk after calving during which Zn balance was negative.ConclusionsLarge adaptations occur in trace metal homeostasis in transition cows in response to changes in dietary intake. High dry-matter intakes, associated with high milk production of dairy cows, combined with current Zn, Mn and Cu supplementation practices may exceed regulatory homeostatic mechanisms resulting in potential body accumulation of Zn, Mn and Cu.Keywords: health; environment; accumulation; regulation; dietary supplementatio

Genetic control of Eucalyptus urophylla and E. grandis resistance to canker caused by Chrysoporthe cubensis

Chrysophorte cubensis induced canker occurs in nearly all tropical and subtropical regions where eucalypts are planted, causing losses in both wood quality and volume productivity, especially so in the warmer and more humid regions of Brazil. The wide inter and intra-specific genetic variability of resistance to canker among Eucalyptus species facilitates the selection of resistant plants. In this study, we evaluated resistance to this pathogen in five Eucalyptus grandis (G) and 15 E. urophylla (U) trees, as well as in 495 individuals from 27 progenies derived from crosses between the trees. In the field, six-months-old test seedlings were inoculated with C. cubensis. Lesion length in the xylem and bark was measured eight months later. The results demonstrated that xylem lesions could preferentially be used for the selection of resistant clones. Eight trees (7 U and 1 G) were susceptible, and the remainder (8 U and 4 G) resistant. Individual narrow and broad sense heritability estimates were 17 and 81%, respectively, thereby suggesting that canker resistance is quantitative and highly dependent on dominance and epistasis