The effect of supplementation with three lactic acid bacteria from bovine origin on growth performance and health status of young calves

Good health in animals that are to be part of human diet during the production chain is key from both production and public health points of view. The use of indigenous microorganisms with probiotic capacity is an alternative for treating and preventing several bovine diseases. This study presents an assessment of the effect of a lactic acid bacteria inoculum from bovine origin integrated by Lactobacillus casei DSPV 318T, Lactobacillus salivarius DSPV 315T and Pediococcus acidilactici DSPV 006T on growth performance and health status of young calves. The 3-microorganism inoculum suspended in a solution of NaCl 0,15M was administered to an experimental group of calves at a daily dose of 109 UFC KgG1 for 35 days. A control group was administered NaCl solution as placebo. The inoculum showed no significant effects, either positive or negative, on the animals´ performance that could have been measured in the conditions in which the study was developed. Perhaps, the lack of evident beneficial effects may have been due to the excellent health status of the animals, the appropriate environmental conditions of the test and the absence of stressing situations during breeding. All evidence shows that the advantages of using probiotics as regards growth performance, health improvement and calf survival could be more easily detected in farms presenting high morbidity and mortality rates mainly produced by diarrhoea syndrome.Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bertozzi, Ezequiel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Zbrun, María Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sequeira, Gabriel Jorge. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Dalla Santina, R.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Rodriguez Armesto, Roberto. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Rosmini, Marcelo Raul. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentin

Respuesta de ratones inoculados con bacterias lácticas de origen bovino a un desafio con Salmonella Dublin

Salmonella dublin produce un desbalance del ecosistema gastrointestinal de los animales de granja. Algunas bacterias lácticas han mostrado capacidad para contrarrestar los efectos de este patógeno. El objetivo del presente trabajo fue estudiar el efecto protector de un inóculo, formado por 3 cepas de bacterias ácido lácticas (BAL) de origen bovino, en ratones desafiados con Salmonella dublin DSPV595T, mediante la observación de lesiones micromorfológicas patognomónicas producidas por el agente patógeno. El microorganismo patógeno se inoculó a 2 grupos de ratones convencionales, uno de los cuales fue previamente tratado con el inóculo BAL. El estudio histopatológico se realizó tanto en los animales que murieron espontáneamente después de haber sido desafiados con el patógeno, como en aquellos que sobrevivieron después de 24 días de haber recibido la Salmonella. El 50% de los animales que pertenecieron al grupo control murieron espontáneamente mientras que en el grupo tratado con BAL esa cifra alcanzó el 32%. Sólo un 10% de las hembras tratadas con BAL murieron espontáneamente mientras que en los machos esa cifra alcanzó al 46,7%. Todos estos individuos presentaron lesiones típicas. El tratamiento con el inóculo brindó protección a los ratones inoculados con Salmonella dublin DSPV 595T en la etapa en la cual se produjeron las muertes espontáneas. Las hembras resultaron mejor protegidas que los machos.Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral; ArgentinaFil: Peralta, C.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Zbrun, María Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Bertozzi, Ezequiel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Martí, Luis Enrique. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Dalla Santina, R.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Sequeira, Gabriel Jorge. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Rosmini, Marcelo Raul. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentin

Molecular Mechanisms Generating and Stabilizing Terminal 22q13 Deletions in 44 Subjects with Phelan/McDermid Syndrome

In this study, we used deletions at 22q13, which represent a substantial source of human pathology (Phelan/McDermid syndrome), as a model for investigating the molecular mechanisms of terminal deletions that are currently poorly understood. We characterized at the molecular level the genomic rearrangement in 44 unrelated patients with 22q13 monosomy resulting from simple terminal deletions (72%), ring chromosomes (14%), and unbalanced translocations (7%). We also discovered interstitial deletions between 17–74 kb in 9% of the patients. Haploinsufficiency of the SHANK3 gene, confirmed in all rearrangements, is very likely the cause of the major neurological features associated with PMS. SHANK3 mutations can also result in language and/or social interaction disabilities. We determined the breakpoint junctions in 29 cases, providing a realistic snapshot of the variety of mechanisms driving non-recurrent deletion and repair at chromosome ends. De novo telomere synthesis and telomere capture are used to repair terminal deletions; non-homologous end-joining or microhomology-mediated break-induced replication is probably involved in ring 22 formation and translocations; non-homologous end-joining and fork stalling and template switching prevail in cases with interstitial 22q13.3. For the first time, we also demonstrated that distinct stabilizing events of the same terminal deletion can occur in different early embryonic cells, proving that terminal deletions can be repaired by multistep healing events and supporting the recent hypothesis that rare pathogenic germline rearrangements may have mitotic origin. Finally, the progressive clinical deterioration observed throughout the longitudinal medical history of three subjects over forty years supports the hypothesis of a role for SHANK3 haploinsufficiency in neurological deterioration, in addition to its involvement in the neurobehavioral phenotype of PMS

Protective Effect of an Inoculum of Lactic Acid Bacteria from Bovine Origin Against Salmonella Serotype Dublin in the Intestinal Tract of Mice

The purpose of this study was to determine the capacitty of an inoculum of lactic acid bacteria (LAB) of bovine origin-constituted by lactobacillus casei DSPV 318T, lactobacillus salivarius DSPV 315T and pediococcus acidilactici DSPV 006T containing about 10(9) cfu dose of each one ot the strains to inhibit in vivo the salmonella serotype dublin DSPV 595T effects and reduce the patologic consequences in a conventional group of mice experimentally challenged with a 2.5 x 10(5) cfu dosed of the pathogen bacteria.Fil: Frizzo, Laureano Sebastian. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Zbrun, María Virginia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Soto, Lorena Paola. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Bertozzi, Ezequiel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sequeira, Gabriel Jorge. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Martí, Luis Enrique. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Dalla Santina, R.. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; ArgentinaFil: Perdigon, Gabriela del Valle. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rosmini, Marcelo Raul. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Salud Pública Veterinaria; Argentin

Computer vision system (CVS): a powerful non-destructive technique for the assessment of red mullet (Mullus barbatus) freshness

The evaluation of fish freshness can be performed using chemical, sensory and physical methods. Besides sensory methods, several instrumental techniques have been applied with the objective of replacing sensory assessment. The aim of this study was to set up and test objective physical methods mainly based on computer vision system (CVS) to assess red mullet (Mullus barbatus) freshness evolution during 10 days of storage, at two different storage temperatures (0 and 4 °C). To check the effectiveness of the purposed physical methods, CVS features (loss in the epidermis pigmentation, development of gill mucus and eye concavity index) and firmness have been compared with chemical trimethylamine content and sensory (QIM) attribute scores. As expected, fish degradation was faster at the higher temperature. Instrumental texture evaluation of fish by penetration test enabled to detect distinctive firmness changes due to onset and resolution of rigor mortis, and the successive tenderization phenomenon. Among CVS parameters, the epidermis pigmentation loss, and particularly the eye shape modification (eye concavity index) evidenced a high sensibility for the estimation of fresh red mullet quality loss, as a function of the two different storage conditions, and a good agreement with trimethylamine content and QIM response evolution