Fortalecimiento de habilidades del pensamiento orientadas a la solución de situaciones problemáticas en los estudiantes del grado sexto de la Institución Educativa Distrital María Mercedes Carranza, mediante el uso de herramientas TIC

183 Páginas.El fortalecimiento de habilidades del pensamiento en los estudiantes del grado sexto de la Institución Educativa Distrital María Mercedes Carranza orientadas a la solución de situaciones problemáticas mediante el uso de herramientas TIC, se basa en dos aspectos necesarios para lograr aprendizajes para toda la vida en los niños y jóvenes, como son habilidades del pensamiento y un método para solucionar dichas situaciones problemáticas; de esta forma se aplicó una pruebas de entrada (diagnóstico), el diseño de un ambiente de aprendizaje y una prueba de salida. Los resultados obtenidos, muestran avances importantes al momento de comprender una situación problemática y desarrollar un proceso lógico y coherente para su solución en la comunidad estudiantil, los cuales trascienden a su ambiente escolar y llega a su entorno social cercano. Los hallazgos identificados, luego de la aplicación del ambiente de aprendizaje, se identificaron puntualmente en 12 estudiantes que culminaron con éxito la aplicación metodológica, en donde se denota en cada uno de ellos un sentido más crítico, analítico y resolutivo para asumir los problemas cotidianos con los que se enfrentan, de esta forma, se pudo demostrar que el fortalecimiento de las habilidades del pensamiento, genera cambios sustanciales en ellos y su entorno social inmediato, lo que ha venido conformando en el grupo seleccionado una cadena de ejemplo y liderazgo de cara a sus demás compañeros.​

European Joint Doctorates: myth or reality?

Today, there is a lack of consensus for the full implementation of common programmes recognizing the “highest” level of higher education in Europe. Even though cotuttelle agreements are widely used for international joint supervision of PhD theses, these are merely bilateral and individual case-based agreements, far away from a real joint degree under a legal framework that establishes the programme. This article aims to describe the experience of the authors in the management and coordination of a joint doctoral programme between 2015 and 2019 and the results obtained from the interrogation of official websites about the reality in Europe concerning such programmes. Our conclusion is that, still in the 21st century, there is a huge gap to be overcome before the existence of Joint International/European Doctorates can be considered an everyday reality. Although various attempts have been made in the last 20 years, there is still a long way to go for Higher Education institutions to integrate all aspects of such programmes, and to make them something more and different than an additional Diploma Supplement. In the authors´ opinion, major efforts must be made by the administrative bodies, although the drive of the academic staff is crucial for success.  [ES] Today, there is a lack of consensus for the full implementation of common programmes recognizing the “highest” level of higher education in Europe. Even though cotuttelle agreements are widely used for international joint supervision of PhD theses, these are merely bilateral and individual case-based agreements, far away from a real joint degree under a legal framework that establishes the programme. This article aims to describe the experience of the authors in the management and coordination of a joint doctoral programme between 2015 and 2019 and the results obtained from the interrogation of official websites about the reality in Europe concerning such programmes. Our conclusion is that, still in the 21st century, there is a huge gap to be overcome before the existence of Joint International/European Doctorates can be considered an everyday reality. Although various attempts have been made in the last 20 years, there is still a long way to go for Higher Education institutions to integrate all aspects of such programmes, and to make them something more and different than an additional Diploma Supplement. In the authors´ opinion, major efforts must be made by the administrative bodies, although the drive of the academic staff is crucial for success.Coy Fuster, P.; Canovas, S.; Van Soom, A.; Bernabo, N.; Lonergan, P.; Schellander, K. (2020). European Joint Doctorates: myth or reality?. En 6th International Conference on Higher Education Advances (HEAd'20). Editorial Universitat Politècnica de València. (30-05-2020):1109-1117. https://doi.org/10.4995/HEAd20.2020.11209OCS1109111730-05-202

Culture Medium and Sex Drive Epigenetic Reprogramming in Preimplantation Bovine Embryos.

Assisted reproductive technologies impact transcriptome and epigenome of embryos and can result in long-term phenotypic consequences. Whole-genome DNA methylation profiles from individual bovine blastocysts in vivo- and in vitro-derived (using three sources of protein: reproductive fluids, blood serum and bovine serum albumin) were generated. The impact of in vitro culture on DNA methylation was analyzed, and sex-specific methylation differences at blastocyst stage were uncovered. In vivo embryos showed the highest levels of methylation (29.5%), close to those produced in vitro with serum, whilst embryos produced in vitro with reproductive fluids or albumin showed less global methylation (25-25.4%). During repetitive element analysis, the serum group was the most affected. DNA methylation differences between in vivo and in vitro groups were more frequent in the first intron than in CpGi in promoters. Moreover, hierarchical cluster analysis showed that sex produced a stronger bias in the results than embryo origin. For each group, distance between male and female embryos varied, with in vivo blastocyst showing a lesser distance. Between the sexually dimorphic methylated tiles, which were biased to X-chromosome, critical factors for reproduction, developmental process, cell proliferation and DNA methylation machinery were included. These results support the idea that blastocysts show sexually-dimorphic DNA methylation patterns, and the known picture about the blastocyst methylome should be reconsidered

Growth analysis and blood profile in piglets born by embryo transfer

©2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ This document is the Published, version of a Published Work that appeared in final form in Research in Veterinary Science. To access the final edited and published work see https://doi.org/10.1016/j.rvsc.2021.11.010Assisted reproductive technologies (ART), besides solving several reproductive problems, it has also been used as a tool to improve the animal productivity that is required for feeding the human population. One of these techniques, the embryo transfer (ET), has presented limitations in the porcine species, which could constrain its use in the porcine industry. To clarify the potential of this technique, we aimed to compare the impact of using ET or artificial insemination (AI) on the phenotype of the offspring during its first days of age, in terms of growth and blood parameters. At birth, the body weight was higher for ET-females than AI-females, but this difference was no longer observed at day 15. On day 3, it was observed a higher concentration of red blood cells, haemoglobin, and haematocrit in females-ET and a higher concentration of white blood cells in both ET-derived piglets (males and females) when compared to AI groups. On day 3, the biochemical analysis showed a higher level of albumin for ET-derived males, and a lower level of bilirubin for ET-females than AI controls. However, all values were within the normal ranges. Our results indicate that piglets derived from ET seem to be phenotypically similar to those born by AI, which provides preliminary evidence that the ET procedure is a safe technique, but additional studies beyond 15 days of life are requested to conclude its global impact. Furthermore, the presented reference values of blood parameters in this species are interesting data for the pig industry

Análisis preliminar de los volátiles almacenados en la glándula sexual de <i>Copitarsia</i> pos. <i>uncilata</i> con miras a su control etológico en cultivos ornamentales

Las polillas del género Copitarsia sp. son polifagas de flores de corte. Estrategias alternativas como el uso de semioquímicos en especial de feromonas, han probado ser exitosas en el control de especies del género en otros países. El presente trabajo busca realizar un primer acercamiento al analizar los compuestos volátiles presentes en la feromona sexual de Copitarsia pos. uncilata que sean candidatos a ser responsables de la atracción sexual, como un primer paso hacia su integración dentro de las estrategias de manejo específico en los cultivos de importancia económica de la Sabana de Bogotá. Para esto se realizó en análisis por cromatografía de gases acoplada a espectrometría de masas de los compuestos del extracto de glándula disectada. Se encontraron varios compuestos con la estructura típica de las feromonas de la superfamilia Noctuidea. Sin embargo hacen falta posteriores evaluaciones en laboratorio y campo para determinar la atracción de estos compuestos. Este proyecto fue financiado por: Vicerrectoría de Investigaciones UMNG-CIAS 1570 Vig- 2015.The moths of the genus Copitarsia sp. are polyphagous of cut flower. Alternative strategies such as the use of semiochemicals especially pheromones, have proven to be successful in controlling species of the genus in other countries. The aim of these paper is to analyze volatile compounds present in the sex pheromone gland of Copitarsia pos. uncilata that are candidates to be responsible for sexual attraction, as a first step towards their integration into specific management strategies in economically important crops of the Bogotá Plateau. It was made an analysis by means of gas chromatography coupled to mass spectrometry to the glandular extract. There were found several compounds with the typical structure of the pheromones of Noctuidea superfamily. Hawever, there are needed further laboratory and field evaluations to assess their effectiveness as an attractant of the species. This proyect was funded by: the Research Vice-Rectory at UMNG-CIAS 1570 validity-2015.Eje: A1 Sistemas de producción de base agroecológica (Trabajos científicos)Facultad de Ciencias Agrarias y Forestale

Análisis preliminar de los volátiles almacenados en la glándula sexual de <i>Copitarsia</i> pos. <i>uncilata</i> con miras a su control etológico en cultivos ornamentales

Las polillas del género Copitarsia sp. son polifagas de flores de corte. Estrategias alternativas como el uso de semioquímicos en especial de feromonas, han probado ser exitosas en el control de especies del género en otros países. El presente trabajo busca realizar un primer acercamiento al analizar los compuestos volátiles presentes en la feromona sexual de Copitarsia pos. uncilata que sean candidatos a ser responsables de la atracción sexual, como un primer paso hacia su integración dentro de las estrategias de manejo específico en los cultivos de importancia económica de la Sabana de Bogotá. Para esto se realizó en análisis por cromatografía de gases acoplada a espectrometría de masas de los compuestos del extracto de glándula disectada. Se encontraron varios compuestos con la estructura típica de las feromonas de la superfamilia Noctuidea. Sin embargo hacen falta posteriores evaluaciones en laboratorio y campo para determinar la atracción de estos compuestos. Este proyecto fue financiado por: Vicerrectoría de Investigaciones UMNG-CIAS 1570 Vig- 2015.The moths of the genus Copitarsia sp. are polyphagous of cut flower. Alternative strategies such as the use of semiochemicals especially pheromones, have proven to be successful in controlling species of the genus in other countries. The aim of these paper is to analyze volatile compounds present in the sex pheromone gland of Copitarsia pos. uncilata that are candidates to be responsible for sexual attraction, as a first step towards their integration into specific management strategies in economically important crops of the Bogotá Plateau. It was made an analysis by means of gas chromatography coupled to mass spectrometry to the glandular extract. There were found several compounds with the typical structure of the pheromones of Noctuidea superfamily. Hawever, there are needed further laboratory and field evaluations to assess their effectiveness as an attractant of the species. This proyect was funded by: the Research Vice-Rectory at UMNG-CIAS 1570 validity-2015.Eje: A1 Sistemas de producción de base agroecológica (Trabajos científicos)Facultad de Ciencias Agrarias y Forestale

Análisis preliminar de los volátiles almacenados en la glándula sexual de <i>Copitarsia</i> pos. <i>uncilata</i> con miras a su control etológico en cultivos ornamentales

Las polillas del género Copitarsia sp. son polifagas de flores de corte. Estrategias alternativas como el uso de semioquímicos en especial de feromonas, han probado ser exitosas en el control de especies del género en otros países. El presente trabajo busca realizar un primer acercamiento al analizar los compuestos volátiles presentes en la feromona sexual de Copitarsia pos. uncilata que sean candidatos a ser responsables de la atracción sexual, como un primer paso hacia su integración dentro de las estrategias de manejo específico en los cultivos de importancia económica de la Sabana de Bogotá. Para esto se realizó en análisis por cromatografía de gases acoplada a espectrometría de masas de los compuestos del extracto de glándula disectada. Se encontraron varios compuestos con la estructura típica de las feromonas de la superfamilia Noctuidea. Sin embargo hacen falta posteriores evaluaciones en laboratorio y campo para determinar la atracción de estos compuestos. Este proyecto fue financiado por: Vicerrectoría de Investigaciones UMNG-CIAS 1570 Vig- 2015.The moths of the genus Copitarsia sp. are polyphagous of cut flower. Alternative strategies such as the use of semiochemicals especially pheromones, have proven to be successful in controlling species of the genus in other countries. The aim of these paper is to analyze volatile compounds present in the sex pheromone gland of Copitarsia pos. uncilata that are candidates to be responsible for sexual attraction, as a first step towards their integration into specific management strategies in economically important crops of the Bogotá Plateau. It was made an analysis by means of gas chromatography coupled to mass spectrometry to the glandular extract. There were found several compounds with the typical structure of the pheromones of Noctuidea superfamily. Hawever, there are needed further laboratory and field evaluations to assess their effectiveness as an attractant of the species. This proyect was funded by: the Research Vice-Rectory at UMNG-CIAS 1570 validity-2015.Eje: A1 Sistemas de producción de base agroecológica (Trabajos científicos)Facultad de Ciencias Agrarias y Forestale

Reproductive Outcomes and Endocrine Profile in Artificially Inseminated versus Embryo Transferred Cows

[EN] The increasing use of in vitro embryo production (IVP) followed by embryo transfer (ET), alongside with cryopreservation of embryos, has risen concerns regarding the possible altered pregnancy rates, calving or even neonatal mortality. One of the hypotheses for these alterations is the current culture conditions of the IVP. In an attempt to better mimic the physiological milieu, embryos were produced with female reproductive fluids (RF) as supplements to culture medium, and another group of embryos were supplemented with bovine serum albumin (BSA) as in vitro control. Embryos were cryopreserved and transferred while, in parallel, an in vivo control (artificial insemination, AI) with the same bull used for IVP was included. An overview on pregnancy rates, recipients’ hormonal levels, parturition, and resulting calves were recorded. Results show much similarity between groups in terms of pregnancy rates, gestation length and calves’ weight. Nonetheless, several differences on hormonal levels were noted between recipients carrying AI embryos especially when compared to BSA. Some calving issues and neonatal mortality were observed in both IVP groups. In conclusion, most of the parameters studied were similar between both types of IVP derived embryos and the in vivo-derived embryos, suggesting that the IVP technology used was efficient enough for the safe production of calvesSIThis research was funded by European Union, Horizon 2020 Marie Sklodowska-Curie Action, grant number REPBIOTECH675526 and as well as by the Ministry of Economy and Competitiveness (Spain), grants number AGL2015-66341-R & AGL2015-70140-R MINECO-FEDER and Fundación Séneca, grant number 20040/GERM/1

Therapeutic antidepressant potential of a conjugated siRNA silencing the serotonin transporter after intranasal administration

A Ferrés-Coy et al.Major depression brings about a heavy socio-economic burden worldwide due to its high prevalence and the low efficacy of antidepressant drugs, mostly inhibiting the serotonin transporter (SERT). As a result, similar to 80% of patients show recurrent or chronic depression, resulting in a poor quality of life and increased suicide risk. RNA interference (RNAi) strategies have been preliminarily used to evoke antidepressant-like responses in experimental animals. However, the main limitation for the medical use of RNAi is the extreme difficulty to deliver oligonucleotides to selected neurons/systems in the mammalian brain. Here we show that the intranasal administration of a sertraline-conjugated small interfering RNA (C-SERT-siRNA) silenced SERT expression/function and evoked fast antidepressant-like responses in mice. After crossing the permeable olfactory epithelium, the sertraline-conjugated-siRNA was internalized and transported to serotonin cell bodies by deep Rab-7-associated endomembrane vesicles. Seven-day C-SERT-siRNA evoked similar or more marked responses than 28-day fluoxetine treatment. Hence, C-SERT-siRNA (i) downregulated 5-HT1A-autoreceptors and facilitated forebrain serotonin neurotransmission, (ii) accelerated the proliferation of neuronal precursors and (iii) increased hippocampal complexity and plasticity. Further, short-term C-SERT-siRNA reversed depressive -like behaviors in corticosterone-treated mice. The present results show the feasibility of evoking antidepressant -like responses by selectively targeting neuronal populations with appropriate siRNA strategies, opening a way for further translational studies.This work was supported by grants from CDTI—Spanish Ministry of Science and Innovation—DENDRIA contribution, 'nLife all rights reserved' (to AB and FA); Instituto de Salud Carlos III PI10/00290 and PI13/01390 (to AB), PI/10/0123 (to JCL) and Centro de Investigación Biomédica en Red de Salud Mental (CIBERSAM); NARSAD Independent Investigator Grant from the Brain & Behavior Research Foundation Grant 20003 (to AB); Ministry of Economy and Competitiveness SAF2012-35183 (to FA) and SAF2011-25020 (to AP); and Generalitat de Catalunya, Secretaria d’Universitat i Recerca del Departament d’Economia i Coneixement (SGR2014) Catalan Government Grant 2009SGR220 (to FA). Some of these grants are co-financed by the European Regional Development Fund 'A way to build Europe'. AF-C is a recipient of a fellowship from Spanish Ministry of Education, Culture and Sport.Peer Reviewe