INDIPRO: INDIzienPROzess – Vertiefende Ermittlungen und Auswertungen von Gewässergütedaten und zusätzlichen Informationen zur Identifikation erforderlicher WRRL-Maßnahmen

Hohe Phosphorgehalte verhindern häufig, dass der gute ökologische Zustand in den Gewässern erreicht wird. In der Schriftenreihe wird eine Vorgehensweise zur Kombination von Daten und zusätzlichen Informationen beschrieben, um den Eintrag für Phosphor einzugrenzen und gezielt Maßnahmen abzuleiten, um den guten ökologische Zustand der Gewässer zu erreichen. Am Beispiel des Keppritzbaches wird das Vorgehen verdeutlicht. Behörden und Institutionen soll es mit der Handlungsanleitung erleichtert werden, eigenständig vertiefende Auswertungen durchzuführen. Redaktionsschluss: 01.05.202

Metabolomics of dietary fatty acid restriction in patients with phenylketonuria

Patients with phenylketonuria (PKU) have to follow a lifelong phenylalanine restricted diet. This type of diet markedly reduces the intake of saturated and unsaturated fatty acids especially long chain polyunsaturated fatty acids (LC-PUFA). Long-chain saturated fatty acids are substrates of mitochondrial fatty acid oxidation for acetyl-CoA production. LC-PUFA are discussed to affect inflammatory and haemostaseological processes in health and disease. The influence of the long term PKU diet on fatty acid metabolism with a special focus on platelet eicosanoid metabolism has been investigated in the study presented here. 12 children with PKU under good metabolic control and 8 healthy controls were included. Activated fatty acids (acylcarnitines C6-C18) in dried blood and the cholesterol metabolism in serum were analyzed by liquid chromatographic tandem mass spectrometry (LC-MS/MS). Fatty acid composition of plasma glycerophospholipids was determined by gas chromatography. LC-PUFA metabolites were analyzed in supernatants by LC-MS/MS before and after platelet activation and aggregation using a standardized protocol. Patients with PKU had significantly lower free carnitine and lower activated fatty acids in dried blood compared to controls. Phytosterols as marker of cholesterol (re-) absorption were not influenced by the dietary fatty acid restriction. Fatty acid composition in glycerophospholipids was comparable to that of healthy controls. However, patients with PKU showed significantly increased concentrations of y-linolenic acid (C18:3n-6) a precursor of arachidonic acid. In the PKU patients significantly higher platelet counts were observed. After activation with collagen platelet aggregation and thromboxane B(2) and thromboxane B(3) release did not differ from that of healthy controls. Long-term dietary fatty acid restriction influenced the intermediates of mitochondrial beta-oxidation. No functional influence on unsaturated fatty acid metabolism and platelet aggregation in patients with PKU was detected

A música como recurso didático-pedagógico para o ensino dos ácidos nucleicos / The music as a didactic-pedagogical resource for nucleic acids teaching

A linguagem musical enquanto forma de expressão artístico-cultural traz consigo uma gama de informações difundidas pelo autor a partir das letras e sons que constituem a sua música, nesta perspectiva, enquanto ferramenta de ensinagem, pode agregar a si os múltiplos saberes intrínsecos ao componente curricular qual se propõe a contemplar. No lecionar das Ciências Biológicas, os docentes encontram o grandioso desafio de trabalhar os saberes da Genética fazendo uso de conceitos outrora depreendidos pelos educandos em anos anteriores, contudo, nem sempre estes termos-chaves estão verdadeiramente concretizados pelos educandos, o que demanda uma abordagem objetiva e diferenciada. Frente a esta realidade o presente estudo busca desenvolver uma obra musical capaz de reapresentar os conceitos essenciais acerca dos ácidos nucleicos, aos discentes do terceiro ano do Ensino Médio, quando da abordagem do conteúdo de Genética pelo professor de Biologia. O produto evidenciado apresentará intrínseco a si informações acerca da composição das moléculas de DNA e RNA, bem como suas características principais, no intuito de evidenciar a eficácia da música para o ensino da Biologia, aos professores leitores deste trabalho e aos educandos que vivenciarem, enquanto público-alvo, a adoção do recurso didático-pedagógico em tela

Regorafenib for recurrent high-grade glioma: a unicentric retrospective analysis of feasibility, efficacy, and toxicity

We describe here 11 consecutive patients with recurrence of high-grade glioma treated with regorafenib at our university medical center. The majority of patients had MGMT promoter methylation (9/11 cases). Regorafenib was given as 2nd line systemic treatment in 6/11 patients and 3rd or higher line treatment in 5/11 patients. The median number of applied cycles was 2 with dosage reductions in 5/11. Response to treatment was observed in 4/11 (PR in 1/11, and SD in 3/11). Median overall survival for the cohort was 16.1 months, median progression-free survival 9.0 months, and median time to treatment failure 3.3 months. Side effects of any CTCAE grade were noted in all patients, hereby 6/11 with CTCAE °III-IV reactions. High-grade side effects were of dermatologic, cardiovascular, and hematologic nature. A mean treatment delay of 57.5 days (range 23–119) was noted between tumor board recommendation and treatment initiation due to the application process for off-label use in this indication. In conclusion, treatment with regorafenib in relapsed high-grade glioma is a feasible treatment option but has to be considered carefully due to the significant side effect profile. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10143-022-01826-z

Proteomic Investigation Uncovers Potential Targets and Target Sites of Pneumococcal Serine-Threonine Kinase StkP and Phosphatase PhpP

Like eukaryotes, different bacterial species express one or more Ser/Thr kinases and phosphatases that operate in various signaling networks by catalyzing phosphorylation and dephosphorylation of proteins that can immediately regulate biochemical pathways by altering protein function. The human pathogen Streptococcus pneumoniae encodes a single Ser/Thr kinase-phosphatase couple known as StkP-PhpP, which has shown to be crucial in the regulation of cell wall synthesis and cell division. In this study, we applied proteomics to further understand the physiological role of pneumococcal PhpP and StkP with an emphasis on phosphorylation events on Ser and Thr residues. Therefore, the proteome of the non-encapsulated D39 strain (WT), a kinase (ΔstkP), and phosphatase mutant (ΔphpP) were compared in a mass spectrometry based label-free quantification experiment. Results show that a loss of function of PhpP causes an increased abundance of proteins in the phosphate uptake system Pst. Quantitative proteomic data demonstrated an effect of StkP and PhpP on the two-component systems ComDE, LiaRS, CiaRH, and VicRK. To obtain further information on the function, targets and target sites of PhpP and StkP we combined the advantages of phosphopeptide enrichment using titanium dioxide and spectral library based data evaluation for sensitive detection of changes in the phosphoproteome of the wild type and the mutant strains. According to the role of StkP in cell division we identified several proteins involved in cell wall synthesis and cell division that are apparently phosphorylated by StkP. Unlike StkP, the physiological function of the co-expressed PhpP is poorly understood. For the first time we were able to provide a list of previously unknown putative targets of PhpP. Under these new putative targets of PhpP are, among others, five proteins with direct involvement in cell division (DivIVA, GpsB) and peptidoglycan biosynthesis (MltG, MreC, MacP)

Host-specific assembly of sponge-associated prokaryotes at high taxonomic ranks

Sponges (Porifera) are abundant and diverse members of benthic filter feeding communities in most marine ecosystems, from the deep sea to tropical reefs. A characteristic feature is the associated dense and diverse prokaryotic community present within the sponge mesohyl. Previous molecular genetic studies revealed the importance of host identity for the community composition of the sponge-associated microbiota. However, little is known whether sponge host-specific prokaryotic community patterns observed at 97% 16S rRNA gene sequence similarity are consistent at high taxonomic ranks (from genus to phylum level). In the present study, we investigated the prokaryotic community structure and variation of 24 sponge specimens (seven taxa) and three seawater samples from Sweden. Results show that the resemblance of prokaryotic communities at different taxonomic ranks is consistent with patterns present at 97% operational taxonomic unit level

Diferenciando espécies crípticas de Drosophila através da eletroforese da enzima fosfatase ácida

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