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Contrasting arbuscular mycorrhizal communities colonizing different host plants show a similar response to a soil phosphorus concentration gradient
High soil phosphorus (P) concentration is frequently shown to reduce root colonization by arbuscular mycorrhizal (AM) fungi, but the influence of P on the diversity of colonizing AM fungi is uncertain.
We used terminal restriction fragment length polymorphism (T-RFLP) of 18S rDNA and cloning to assess diversity of AM fungi colonizing maize (Zea mays), soybean (Glycene max) and field violet (Viola arvensis) at three time points in one season along a P gradient of 10–280 mg l−1 in the field.
Percentage AM colonization changed between sampling time points but was not reduced by high soil P except in maize. There was no significant difference in AM diversity between sampling time points. Diversity was reduced at concentrations of P > 25 mg l−1, particularly in maize and soybean. Both cloning and T-RFLP indicated differences between AM communities in the different host species. Host species was more important than soil P in determining the AM community, except at the highest P concentration.
Our results show that the impact of soil P on the diversity of AM fungi colonizing plants was broadly similar, despite the fact that different plants contained different communities. However, subtle differences in the response of the AM community in each host were evident
Event Rates, Hospital Utilization, and Costs Associated with Major Complications of Diabetes: A Multicountry Comparative Analysis
Philip Clarke and colleagues examined patient-level data for over 11,000 participants with type 2 diabetes from 20 countries and find that major complications of diabetes significantly increased hospital use and costs across settings
Lost in translation? The potential psychobiotic Lactobacillus rhamnosus (JB-1) fails to modulate stress or cognitive performance in healthy male subjects
Background: Preclinical studies have identified certain probiotics as psychobiotics a live microorganisms with a potential mental health benefit. Lactobacillus rhamnosus (JB-1) has been shown to reduce stress-related behaviour, corticosterone release and alter central expression of GABA receptors in an anxious mouse strain. However, it is unclear if this single putative psychobiotic strain has psychotropic activity in humans. Consequently, we aimed to examine if these promising preclinical findings could be translated to healthy human volunteers. Objectives: To determine the impact of L. rhamnosus on stress-related behaviours, physiology, inflammatory response, cognitive performance and brain activity patterns in healthy male participants. An 8 week, randomized, placebo-controlled, cross-over design was employed. Twenty-nine healthy male volunteers participated. Participants completed self-report stress measures, cognitive assessments and resting electroencephalography (EEG). Plasma IL10, IL1β, IL6, IL8 and TNFα levels and whole blood Toll-like 4 (TLR-4) agonist-induced cytokine release were determined by multiplex ELISA. Salivary cortisol was determined by ELISA and subjective stress measures were assessed before, during and after a socially evaluated cold pressor test (SECPT). Results: There was no overall effect of probiotic treatment on measures of mood, anxiety, stress or sleep quality and no significant effect of probiotic over placebo on subjective stress measures, or the HPA response to the SECPT. Visuospatial memory performance, attention switching, rapid visual information processing, emotion recognition and associated EEG measures did not show improvement over placebo. No significant anti-inflammatory effects were seen as assessed by basal and stimulated cytokine levels. Conclusions: L. rhamnosus was not superior to placebo in modifying stress-related measures, HPA response, inflammation or cognitive performance in healthy male participants. These findings highlight the challenges associated with moving promising preclinical studies, conducted in an anxious mouse strain, to healthy human participants. Future interventional studies investigating the effect of this psychobiotic in populations with stress-related disorders are required
Stellar Populations in Three Outer Fields of the LMC
We present HST photometry for three fields in the outer disk of the LMC
extending approximately four magnitudes below the faintest main sequence
turnoff. We cannot detect any strongly significant differences in the stellar
populations of the three fields based on the morphologies of the
color-magnitude diagrams, the luminosity functions, and the relative numbers of
stars in different evolutionary stages. Our observations therefore suggest
similar star formation histories in these regions, although some variations are
certainly allowed. The fields are located in two regions of the LMC: one is in
the north-east field and two are located in the north-west. Under the
assumption of a common star formation history, we combine the three fields with
ground-based data at the same location as one of the fields to improve
statistics for the brightest stars. We compare this stellar population with
those predicted from several simple star formation histories suggested in the
literature, using a combination of the R-method of Bertelli et al (1992) and
comparisons with the observed luminosity function. The only model which we
consider that is not rejected by the observations is one in which the star
formation rate is roughly constant for most of the LMC's history and then
increases by a factor of three about 2 Gyr ago. Such a model has roughly equal
numbers of stars older and younger than 4 Gyr, and thus is not dominated by
young stars. This star formation history, combined with a closed box chemical
evolution model, is consistent with observations that the metallicity of the
LMC has doubled in the past 2 Gyr.Comment: 30 pages, includes 10 postscript figures. Figure 1 avaiable at
ftp://charon.nmsu.edu/pub/mgeha/LMC. Accepted for publication in Astronomical
Journa
Investigation, Modeling, and Analysis of Integrated Metroplex Arrival and Departure Coordination Concepts
This work involves the development of a concept that enhances integrated metroplex arrival and departure coordination, determines the temporal (the use of time separation for aircraft sharing the same airspace resources) and spatial (the use of different routes or vertical profiles for aircraft streams at any given time) impact of metroplex traffic coordination within the National Airspace System (NAS), and quantifies the benefits of the most desirable metroplex traffic coordination concept. Researching and developing metroplex concepts is addressed in this work that broadly applies across the range of airspace and airport demand characteristics envisioned for NextGen metroplex operations. The objective of this work is to investigate, formulate, develop models, and analyze an operational concept that mitigates issues specific to the metroplex or that takes advantage of unique characteristics of metroplex airports to improve efficiencies. The concept is an innovative approach allowing the NAS to mitigate metroplex interdependencies between airports, optimize metroplex arrival and departure coordination among airports, maximize metroplex airport throughput, minimize delay due to airport runway configuration changes, increase resiliency to disruptions, and increase the tolerance of the system to degrade gracefully under adverse conditions such as weather, traffic management initiatives, and delays in general
Malaria Infection and Anemia Prevalence in Zambia's Luangwa District: An Area of Near-Universal Insecticide-Treated Mosquito Net Coverage
We examined the relationship between insecticide-treated mosquito nets (ITNs), malaria parasite infection, and severe anemia prevalence in children in Luangwa District, Zambia, an area with near-universal ITN coverage, at the end of the 2008 and 2010 malaria transmission seasons. Malaria parasite infection prevalence among children < 5 years old was 9.7% (95% confidence interval [CI] = 8.0–11.4%) over both survey years. Prevalence of severe anemia among children 6–59 months old was 6.9% (95% CI = 5.4–8.5%) over both survey years. Within this context of near-universal ITN coverage, we were unable to detect a significant association between malaria parasite or severe anemia prevalence and ITNs (possession and use). In addition to maintaining universal ITN coverage, it will be essential for the malaria control program to achieve high ITN use and laboratory diagnosis and treatment of all fevers among all age groups to further reduce the malaria burden in this area
Bovine Tuberculosis in Free-Ranging Carnivores from Michigan
During a survey of carnivores and omnivores for bovine tuberculosis conducted in Michigan (USA) since 1996, Mycobacterium bovis was cultured from lymph nodes pooled from six coyotes (Canis latrans) (four adult female, two adult male), two adult male raccoons (Procyon lotor), one adult male red fox (Vulpes vulpes), and one 1.5-yr-old male black bear (Ursus americanus). One adult, male bobcat (Felis rufus) with histologic lesions suggestive of tuberculosis was negative on culture but positive for organisms belonging to the Mycobacterium tuberculosis complex when tested by polymerase chain reaction. All the tuberculous animals were taken from three adjoining counties where M. bovis is known to be endemic in the free-ranging white-tailed deer (Odocoileus virginianus) population. There were two coyotes, one raccoon, one red fox, and one bobcat infected in Alpena county. Montmorency County had two coyotes and one raccoon with M. bovis. Two coyotes and a bear were infected from Alcona County. These free-ranging carnivores/omnivores probably became infected with M. bovis through consumption of tuberculous deer. Other species included in the survey were opossum (Didelphis virginiana), gray fox (Urocyon cinereoargenteus), and badger (Taxidea taxus); these were negative for M. bovis
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