Microcontroller-Based Sinusoidal Voltage Generation for Electrical Bio-Impedance Spectroscopy Applications

A sinusoidal voltage wave generator is proposed based on the use of micro- processor digital signals with programmable duty-cycles, with application to real-time Electrical Cell-substrate Impedance Spectroscopy (ECIS) assays in cell cultures. The working principle relies on the time convolution of the programmed microcontroller (μC) digital signals. The expected frequency is easily tuned on the bio-impedance spectroscopy range [100 Hz, 1 MHz] thanks to the μC clock frequency selection. This system has been simulated and tested on the 8 bits μC Arduino™ Uno with ATmega328 version. Results obtained prove that only three digital signals are required to fit the general specification in ECIS experiments, below 1% THD accuracy, and show the appropriateness of the system for the real-time monitoring of this type of biological experiments.Spanish founded Project: TEC 2013- 46242-C3-1-P: Integrated Microsystem for Cell Culture AssaysFEDE

Plan de Establecimiento de la Arquitectura Tecnológica de la Comunicación y Almacenamiento de Datos a la Empresa OBRAS DE INGENIERIA, S.A OBRINSA, en el periodo del 1 de Septiembre del 2015 al 15 de Febrero del 2016

En el presente trabajo de seminario de graduación se realizaron los primeros dos grupos de procesos para la realización de un proyecto según el Instituto de Gerencia de Proyectos, los cuales son: Grupo de Procesos de Iniciación, y Grupo de Procesos de Planificación. Para la elaboración del proyecto Plan de Establecimiento de la arquitectura tecnológica de la comunicación y almacenamiento de datos a la empresa “OBRAS DE INGENIERIA, S.A” “OBRINSA” se emplearon diferentes técnicas y herramientas propuestas en la guía de fundamentos para la gerencia de proyectos, tales como: reuniones y entrevistas con la partes interesadas, Plantillas de Plan de Riesgos, Plantillas para la gestión de Interesados, Plantillas para la gestión del Alcance, Estimación de costos, herramientas automatizadas tales como el MS PROJECT 2013, WBS CHART PRO, Microsoft Excel, entre otras. El proyecto cuenta con el plan de Gestión de alcance, plan de gestión de tiempo, Gestión de comunicaciones, Gestión de Recursos Humanos, Gestión de Costo, Gestión de Interesados. El equipo del proyecto está conformado por un Gerente de Proyecto, un Administrador de Redes y un Administrador del Centro de Datos. La ejecución del proyecto está estimado en un tiempo de 95 días laborales y su costo estimado es de C$496,408.5; Cumpliendo de esta forma con los requerimientos solicitados por el cliente

CTS y medios de comunicación social: Algunas perspectivas para su análisis

Este artículo pretende integrar en las distintas líneas de investigación sobre Ciencia, Tecnología y Sociedad (CTS) el estudio de los medios de comunicación social desde distintas perspectivas de análisis: el ámbito histórico, el ámbito social, el ámbito político, el ámbito comunicativo y el ámbito cultural, especialmente considerando la dimensión tecnológica del proceso de la acción comunicativa. Es necesario vincular el análisis de los medios de comunicación y de las formas de la comunicación social desde una perspectiva CTS porque el control social de la ciencia y la tecnología tiene que pasar por informar mejor a los ciudadanos, así como articular cauces institucionales para que los ciudadanos expresen su voluntad y tengan criterios para entender la realidad que les rodea en la sociedad de la información

Cell-culture real time monitoring based on bio-impedance measurements

This paper proposes the application of a cell-microelectrode model in cell biometry experiments, using the cell-electrode area overlap as its main parameter. The model can be applied to cell size identification and cell count, and further extended to study cell growth and dosimetry protocols. Experiments have been conducted in AA8 cell line, obtaining promising results.Junta de Andalucía P0-TIC-538

Detection and Quantitation of Olive Pollen Allergen Isoforms Using 2-D Western Blotting

This work was supported by the Spanish Ministry of Science and Innovation (MICINN) (ERDF-cofinanced projects AGL2008-00517, BFU2011-22779 and PIE-200840I186) and the Junta de Andalucía (ERDF-cofinanced projects P2010-CVI5767 and P2010-AGR6274). K. Zienkiewicz thanks the CSIC for providing JAEdoc grant funding.Peer reviewe

Emotions Detection based on a Single-electrode EEG Device

The study of emotions using multiple channels of EEG represents a widespread practice in the field of research related to brain computer interfaces (Brain Computer Interfaces). To date, few studies have been reported in the literature with a reduced number of channels, which when used in the detection of emotions present results that are less accurate than the rest. To detect emotions using an EEG channel and the data obtained is useful for classifying emotions with an accuracy comparable to studies in which there is a high number of channels, is of particular interest in this research framework. This article uses the Neurosky Maindwave device; which has a single electrode to acquire the EEG signal, Matlab software and IBM SPSS Modeler; which process and classify the signals respectively. The accuracy obtained in the detection of emotions in relation to the economic resources of the hardware dedicated to the acquisition of EEG signal is remarkable

Disposable masks: Disinfection and sterilization for reuse, and non-certified manufacturing, in the face of shortages during the COVID-19 pandemic.

The COVID-19 pandemic is posing a huge global health threat. To deal with this problem, in addition to research and work in the medical field, the main health measures being taken in the workplace and at home involve the establishment of safety protocols, which include distance measures, hygiene and the use of personal protective equipment, such as masks, etc. The WHO still does not recommend the use of masks for the general population. However, their successful use in China, South Korea and the Czech Republic has encouraged their widespread use, and the shortage that already existed. This has caused that companies and individuals are looking at the best way to reuse them, and to manufacture, homemade or not, of non-certified masks. This paper is based on two objectives: to consult the scientific literature to identify the main strategies for disinfecting them, and to determine the effectiveness of non-certified disposable masks. A rapid review has been conducted in which the main publications and other information available online have been analyzed. Results showed that the most promising methods are those that use hydrogen peroxide vapor, ultraviolet radiation, moist heat, dry heat and ozone gas. Soapy water, alcohol, bleach immersion, ethylene oxide, ionizing radiation, microwave, high temperature, autoclave or steam are not fully recommended. Regarding the effectiveness of surgical masks compared to PPE, the former have been seen to be slightly less effective than PPE. As for other types of masks the effectiveness of homemade or non-certified masks is very low

Involvement of Climatic Factors in the Allergen Expression in Olive Pollen

This work was supported by the Spanish Ministry of Science and Innovation (MICINN) (ERDF-cofinanced projects AGL2008-00517, BFU2011-22779 and PIE-200840I186) and the Junta de Andalucía (ERDF-cofinanced projects P2010-CVI5767 and P2010-AGR6274).Peer reviewe