5 research outputs found
Reproductive function and control of the quality of Eurasian perch, Perca fluviatilis
LâamĂ©lioration des performances de reproduction des poissons dâĂ©levage nĂ©cessite de dĂ©terminer les facteurs intrinsĂšques et extrinsĂšques influençant la qualitĂ© des gamĂštes dâune part, et de dĂ©finir des paramĂštres fiables permettant de prĂ©dire les performances de reproduction dâautre part. Notre objectif est donc de comprendre le dĂ©terminisme multifactoriel de la reproduction chez la perche commune, Perca fluviatilis. Quatre facteurs nutritionnels (type dâaliment et taux de rationnement distribuĂ©s lors des phases dâinduction et de vernalisation) et 3 facteurs populationnels (poids initial, origine gĂ©ographique, niveau de domestication) ont Ă©tĂ© testĂ©s. Une diffĂ©rence de rĂ©ponse entre les sexes a Ă©tĂ© observĂ©e. Le type dâaliment distribuĂ© en vernalisation et le poids initial ont modifiĂ© lâĂ©tat gĂ©nĂ©ral des femelles. Les mĂąles ont plutĂŽt Ă©tĂ© sensibles aux taux de rationnement et Ă lâorigine gĂ©ographique. LâĂ©tude des performances de reproduction a montrĂ© que le taux de ponte Ă©tait sous lâinfluence de lâinteraction entre le type dâaliment distribuĂ© en induction et en vernalisation, tandis que lâorigine gĂ©ographique a modulĂ© la date de ponte. La rĂ©gulation des performances de reproduction est donc un mĂ©canisme complexe sous lâinfluence simultanĂ©e de plusieurs facteurs. La seconde partie de ce travail concerne la recherche de marqueurs prĂ©dictifs de la qualitĂ© des ovules. Nous avons dâabord montrĂ© que peu de paramĂštres morpho-anatomiques des pontes ou ovules sont des prĂ©dicateurs fiables. Cependant, lâanalyse protĂ©omique a permis de mettre en Ă©vidence plusieurs protĂ©ines exprimĂ©es diffĂ©remment selon la qualitĂ© des pontes, pouvant jouer le rĂŽle de biomarqueurs de qualitĂ© des ovulesImproving fish reproduction in breeding conditions implies to understand intrinsic and extrinsic factors influencing gametes quality on the one hand and to define relevant parameters allowing the prediction of fish reproductive performance on the other hand. Our goal was thus to understand the multifactorial determinism of the common perch (Perca fluviatilis) reproduction. Four nutritional factors (type of food and rate of rationing used either during the induction or vernalization phases) and 3 populational factors (initial weight, geographic origin and domestication level of breeders) have been tested. Data show different responses between females and males. type of food during wintering phase and initial broodstock weigh modified female condition. Males have been sensitive to rationing during wintering phase as well as geographical origin. Data show also that spawning rate was under the influence of interaction between kind of food during wintering phase and induction whereas geographical origin modulated the spawning date. The regulation of the performance reproduction is also a complex mechanism influenced by several factors. The second part of this work consisted on the research of parameters potentially predictive of ova quality. Firstly, our work shows that morphometric parameters measured before the fertilization are poorly relevant to predict reproductive performance. However, the proteomic analysis of several spawn allowed us to highlight proteins differently expressed according to the spawn quality, such proteins could be ova quality biomarker
Fonction de reproduction et régulation de la qualité chez la perche commune, Perca fluviatilis
Improving fish reproduction in breeding conditions implies to understand intrinsic and extrinsic factors influencing gametes quality on the one hand and to define relevant parameters allowing the prediction of fish reproductive performance on the other hand. Our goal was thus to understand the multifactorial determinism of the common perch (Perca fluviatilis) reproduction. Four nutritional factors (type of food and rate of rationing used either during the induction or vernalization phases) and 3 populational factors (initial weight, geographic origin and domestication level of breeders) have been tested. Data show different responses between females and males. type of food during wintering phase and initial broodstock weigh modified female condition. Males have been sensitive to rationing during wintering phase as well as geographical origin. Data show also that spawning rate was under the influence of interaction between kind of food during wintering phase and induction whereas geographical origin modulated the spawning date. The regulation of the performance reproduction is also a complex mechanism influenced by several factors. The second part of this work consisted on the research of parameters potentially predictive of ova quality. Firstly, our work shows that morphometric parameters measured before the fertilization are poorly relevant to predict reproductive performance. However, the proteomic analysis of several spawn allowed us to highlight proteins differently expressed according to the spawn quality, such proteins could be ova quality biomarkers.L'amélioration des performances de reproduction des poissons d'élevage nécessite de déterminer les facteurs intrinsÚques et extrinsÚques influençant la qualité des gamÚtes d'une part, et de définir des paramÚtres fiables permettant de prédire les performances de reproduction d'autre part. Notre objectif est donc de comprendre le déterminisme multifactoriel de la reproduction chez la perche commune, Perca fluviatilis. Quatre facteurs nutritionnels (type d'aliment et taux de rationnement distribués lors des phases d'induction et de vernalisation) et 3 facteurs populationnels (poids initial, origine géographique, niveau de domestication) ont été testés. Une différence de réponse entre les sexes a été observée. Le type d'aliment distribué en vernalisation et le poids initial ont modifié l'état général des femelles. Les mùles ont plutÎt été sensibles aux taux de rationnement et à l'origine géographique. L'étude des performances de reproduction a montré que le taux de ponte était sous l'influence de l'interaction entre le type d'aliment distribué en induction et en vernalisation, tandis que l'origine géographique a modulé la date de ponte. La régulation des performances de reproduction est donc un mécanisme complexe sous l'influence simultanée de plusieurs facteurs. La seconde partie de ce travail concerne la recherche de marqueurs prédictifs de la qualité des ovules. Nous avons d'abord montré que peu de paramÚtres morpho-anatomiques des pontes ou ovules sont des prédicateurs fiables. Cependant, l'analyse protéomique a permis de mettre en évidence plusieurs protéines exprimées différemment selon la qualité des pontes, pouvant jouer le rÎle de biomarqueurs de qualité des ovules
Fonction de reproduction et régulation de la qualité chez la perche commune, Perca fluviatilis
L amélioration des performances de reproduction des poissons d élevage nécessite de déterminer les facteurs intrinsÚques et extrinsÚques influençant la qualité des gamÚtes d une part, et de définir des paramÚtres fiables permettant de prédire les performances de reproduction d autre part. Notre objectif est donc de comprendre le déterminisme multifactoriel de la reproduction chez la perche commune, Perca fluviatilis. Quatre facteurs nutritionnels (type d aliment et taux de rationnement distribués lors des phases d induction et de vernalisation) et 3 facteurs populationnels (poids initial, origine géographique, niveau de domestication) ont été testés. Une différence de réponse entre les sexes a été observée. Le type d aliment distribué en vernalisation et le poids initial ont modifié l état général des femelles. Les mùles ont plutÎt été sensibles aux taux de rationnement et à l origine géographique. L étude des performances de reproduction a montré que le taux de ponte était sous l influence de l interaction entre le type d aliment distribué en induction et en vernalisation, tandis que l origine géographique a modulé la date de ponte. La régulation des performances de reproduction est donc un mécanisme complexe sous l influence simultanée de plusieurs facteurs. La seconde partie de ce travail concerne la recherche de marqueurs prédictifs de la qualité des ovules. Nous avons d abord montré que peu de paramÚtres morpho-anatomiques des pontes ou ovules sont des prédicateurs fiables. Cependant, l analyse protéomique a permis de mettre en évidence plusieurs protéines exprimées différemment selon la qualité des pontes, pouvant jouer le rÎle de biomarqueurs de qualité des ovulesImproving fish reproduction in breeding conditions implies to understand intrinsic and extrinsic factors influencing gametes quality on the one hand and to define relevant parameters allowing the prediction of fish reproductive performance on the other hand. Our goal was thus to understand the multifactorial determinism of the common perch (Perca fluviatilis) reproduction. Four nutritional factors (type of food and rate of rationing used either during the induction or vernalization phases) and 3 populational factors (initial weight, geographic origin and domestication level of breeders) have been tested. Data show different responses between females and males. type of food during wintering phase and initial broodstock weigh modified female condition. Males have been sensitive to rationing during wintering phase as well as geographical origin. Data show also that spawning rate was under the influence of interaction between kind of food during wintering phase and induction whereas geographical origin modulated the spawning date. The regulation of the performance reproduction is also a complex mechanism influenced by several factors. The second part of this work consisted on the research of parameters potentially predictive of ova quality. Firstly, our work shows that morphometric parameters measured before the fertilization are poorly relevant to predict reproductive performance. However, the proteomic analysis of several spawn allowed us to highlight proteins differently expressed according to the spawn quality, such proteins could be ova quality biomarkersNANCY-INPL-Bib. électronique (545479901) / SudocSudocFranceF
Primary production, respiration and calcification of the temperate free-living coralline alga Lithothamnion corallioides
International audienceCalcification and primary production responses to irradiance in the temperate coralline alga Lithothamnion corallioides were measured in summer 2004 and winter 2005 in the Bay of Brest. Coralline algae were incubated in dark and clear bottles exposed to different irradiances. Net primary production reached 1.5 ÎŒmol C gâ1 dry wt hâ1 in August and was twice as high as in January-February. Dark respiration showed significant seasonal variations, being three-fold higher in summer. Maximum calcification varied from 0.6 ÎŒmol gâ1 dry wt hâ1 in summer 2004 to 0.4 ÎŒmol gâ1 dry wt hâ1 in winter 2005. According to P-E curves and the daily course of irradiance, estimated daily net production and calcification reached 131 ÎŒg C gâ1 dry wt and 970 ÎŒg CaCO3 gâ1 dry wt in summer 2004, and 36 ÎŒg C gâ1 dry wt and 336 ÎŒg CaCO3 gâ1 dry wt in winter 2005. The net primary production of natural L. corallioides populations in shallow waters was estimated at 10-600 g C mâ2 yâ1, depending on depth and algal biomass. The mean annual calcification of L. corallioides populations varied from 300 to 3000 g CaCO3 mâ2. These results are similar to those reported for tropical coralline algae in terms of carbon and carbonate productivity. Therefore, L. corallioides can be considered as a key element of carbon and carbonate cycles in the shallow coastal waters where they live
An amphibious mode of life in the intertidal zone: aerial and underwater contribution of Chthamalus montagui to CO2 fluxes
International audienceThe contribution of the intertidal barnacle Chthamalus montagui to CO2 fluxes via respiration and calcification was measured both in the air and underwater. The mean biomass of the species was 44.92 g ash-free dry weight (AFDW) mâ2 on the coast of Brittany, France. Underwater respiration, determined from changes in dissolved inorganic carbon (DIC), fluctuated from 6.14 ”mol gâ1 hâ1 in winter to 13.37 ”mol gâ1 hâ1 in summer. The contribution of C. montagui respiration to DIC fluxes for an average daily immersion time of 8 h was 3.21 mmol mâ2 dâ1. Mean aerial CO2 respiration was estimated at 7.60 ”mol gâ1 hâ1 using an infrared gas analyser, corresponding to 5.46 mmol mâ2 dâ1 if the mean daily emersion time is 16 h. Net calcification was positive, with a mean value of 1.01 ”mol gâ1 hâ1, corresponding to a CO2 flux of 0.25 mmol mâ2 dâ1. The total mean daily emission of CO2 by C. montagui populations was 8.92 mmol mâ2 dâ1. The annual carbon production by the species was 39.07 g mâ2 yrâ1 with relative contributions by aerial respiration, underwater respiration and net calcification of 61, 36 and 3%, respectively. The daily ratio of aerial:underwater carbon emission was 1.7, emphasizing the prevalence of aerial respiration and the metabolic adaptation of C. montagui to amphibious lif