Differences in the Distribution of Peripheral Blood Leukocyte and Lymphocyte Subsets in Pulmonary Tuberculosis Patients

Introduction: It has been suggested that individuals exposed to Mycobacterium tuberculosis can eliminate the infection through the innate immune response without the participation of the acquired immunity. However, T helper type 1 immune response is critical to control persistent infection. Objective: The purpose of this study was to evaluate the distribution of leukocyte and lymphocyte subsets in peripheral blood of individuals with pulmonary tuberculosis (TB) and positive Tuberculin Skin Test (TST). Methodology: In order to achieve that goal, we have collected blood sample from thirty-four treatment-naïve TB patients, fifty TST-positive and forty-one TST-negative individuals. Results: The evaluation has shown a significant reduction in the number of total lymphocytes, B cells, CD4+ and CD8+ T cells and percentage increase of NK cells, neutrophils and monocytes in TB patients, when compared to TSTpositive and TST-negative individuals. There was no statistical difference for leucocyte and lymphocyte subsets between TST-positive and TST-negative groups. Peripheral blood white cell counts change significantly at diagnosis. Conclusion: The quantification of these cells may support the diagnosis and monitoring of patients with pulmonary tuberculosis

Interferon-gamma release assay performance in northeastern Brazil: influence of the IFNG+874 A>T polymorphism

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-13T14:13:22Z No. of bitstreams: 1 Carneiro VL Interferon-gamma release assay ....pdf: 865425 bytes, checksum: 49fe950c24d031c54cbc006fce65c921 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-13T14:23:58Z (GMT) No. of bitstreams: 1 Carneiro VL Interferon-gamma release assay ....pdf: 865425 bytes, checksum: 49fe950c24d031c54cbc006fce65c921 (MD5)Made available in DSpace on 2018-08-13T14:23:58Z (GMT). No. of bitstreams: 1 Carneiro VL Interferon-gamma release assay ....pdf: 865425 bytes, checksum: 49fe950c24d031c54cbc006fce65c921 (MD5) Previous issue date: 2018Conselho Nacional de Desenvolvimento Científico e Tecnológico (INCT-DT/MCT/CNPq 573839/2008-5) and Fundação de Amparo à Pesquisa do Estado da Bahia (PET035/2013).Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Fundação José Silveira. Instituto Brasileiro para a Investigação da Tuberculose. Salvador, BA, BrazilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilLatent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG+874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG+874 A/T polymorphism on interferon-gamma test performance. Methods: Patients diagnosed with pulmonary tuberculosis (75), volunteers with positivetuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no his-tory of contact with tuberculosis (57) were evaluated regarding the IFNG + 874 genotype andthe IFN- levels in whole blood cultures performed using an interferon-gamma commercialkit (QuantiFERON-TB®Gold In-Tube).Results: IFN- production was not influenced by the IFNG + 874 genotype, regardless of anti-gen or mitogen-based stimulation, which suggests that other genes may influence IFN- production in response to mycobacteria. The IFNG + 874 polymorphism was found to exertno influence over QFT-IT test sensitivity in our study

Genetic variants in RORA are associated with asthma and allergy markers in an admixed population

Barreto, Mauricio Lima. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.O artigo encontra-se disponível em acesso aberto para download no site do Editor.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-15T14:07:26Z No. of bitstreams: 1 Lima LC Genetic variants in RORA are associated .....pdf: 1052100 bytes, checksum: 5716ce205bb9f5085a139da0eecdb181 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-08-15T14:16:45Z (GMT) No. of bitstreams: 1 Lima LC Genetic variants in RORA are associated .....pdf: 1052100 bytes, checksum: 5716ce205bb9f5085a139da0eecdb181 (MD5)Made available in DSpace on 2018-08-15T14:16:45Z (GMT). No. of bitstreams: 1 Lima LC Genetic variants in RORA are associated .....pdf: 1052100 bytes, checksum: 5716ce205bb9f5085a139da0eecdb181 (MD5) Previous issue date: 2018CNPq National Research Council - Brazil and FAPESB State of Bahia, Brazil Research Council.Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto Multidisciplinar em Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade do Estado da Bahia. Departamento de Ciências da Vida. Salvador, BA, Brasil.Asthma and allergy affect hundreds of millions of people from childhood to old age. In most of them, the inflammatory process of respiratory allergies involves the participation of type 2 cytokines, derived from T helper-2 (Th2)-cell, and Group 2 Innate Lymphoid (ILC2) Cells. An efficient memory Th2 cell response is dependent on IL-13 produced by ILC2s, causing allergic lung inflammation and elevated serum levels of immunoglobulin E. ILC2 cells are derived from common lymphoid progenitors and their growing depends on the transcription factor RORA. The aim of this work was to identify genetic variants in RORA associated with asthma phenotypes and allergy markers. Genomic DNA samples of 1246 individuals participating from Social Changes Asthma and Allergy in Latin America Program (SCAALA) have been genotyped using Illumina Human 2.5 Omni Beadchip. Logistics regressions have been performed to analyze the association among RORA variants and asthma, skin prick tests (SPT), specific IgE and type 2 cytokine production. Twelve single nucleotide variants (SNVs) were significantly associated with atopy (P < 0.01), in which four of them, rs10162630, rs17191519, rs17270243, and rs55796775 and their haplotypes were strongly and positively associated (P < 0.001). Furthermore, these variants increased the RORA gene expression in silico analysis. Other SNVs in RORA were associated with allergy markers, atopic and non-atopic asthma. Therefore, it is believed that variants in RORA gene may influence immunologic features of asthma and allergies and could be possible targets for future treatment of allergic diseases

Interferon-gamma release assay performance in northeastern Brazil: influence of the IFNG + 874 A>T polymorphism

ABSTRACT Introduction Latent tuberculosis infection diagnosis based on the release of interferon-gamma in cultures of peripheral blood cells stimulated with Mycobacterium tuberculosis antigens has replaced the tuberculin skin test in many countries with low tuberculosis prevalence. The IFN-γ production can be influenced by genetic polymorphisms, of which the IFNG + 874 (rs62559044) locus is the most studied. We investigated the possible influence of the IFNG + 874 A/T polymorphism on interferon-gamma test performance. Methods Patients diagnosed with pulmonary tuberculosis (75), volunteers with positive tuberculin skin test (70) and healthy volunteers with negative tuberculin skin test and no history of contact with tuberculosis (57) were evaluated regarding the IFNG + 874 genotype and the IFN-γ levels in whole blood cultures performed using an interferon-gamma commercial kit (QuantiFERON-TB® Gold In-Tube). Results IFN-γ production was not influenced by the IFNG + 874 genotype, regardless of antigen or mitogen-based stimulation, which suggests that other genes may influence IFN-γ production in response to mycobacteria. The IFNG + 874 polymorphism was found to exert no influence over QFT-IT test sensitivity in our study. Conclusions The IFNG + 874 polymorphism was not shown to influence QuantiFERON-TB® Gold In-Tube test performance in an admixed population from northeastern Brazil