Gene phylogenies and protein–protein interactions: possible artifacts resulting from shared protein interaction partners

The study of gene families critically depends on the correct reconstruction of gene genealogies, as for instance in the case of transcription factor genes like Hox genes and Dlx gene families. Proteins belonging to the same family are likely to share some of the same protein interaction partners and may thus face a similar selective environment. This common selective environment can induce co-evolutionary pressures and thus can give rise to correlated rates and patterns of evolution among members of a gene family. In this study, we simulate the evolution of a family of sequences which share a set of interaction partners. Depending on the amount of sequence dedicated to protein–protein interaction and the relative rate parameters of sequence evolution three outcomes are possible: if the fraction of the sequence dedicated to interaction with common co-factors is low and the time since divergence is small, the trees based on sequence information tend to be correct. If the time since gene duplication is long two possible outcomes are observed in our simulations. If the rate of evolution of the interaction partner is small compared to the rate of evolution of the focal protein family, the reconstructed trees tend towards star phylogenies. As the rate of evolution of the interaction partner approaches that of the focal protein family the reconstructed phylogenies tend to be incorrectly resolved. We conclude that the genealogies of gene families can be hard to estimate, in particular if the proteins interact with a conserved set of binding partners, as is likely the case for transcription factors

Bounded fitness landscapes and the evolution of the linguistic diversity

A simple spatial computer simulation model was recently introduced to study the evolution of the linguistic diversity. The model considers processes of selective geographic colonization, linguistic anomalous diffusion and mutation. In the approach, we ascribe to each language a fitness function which depends on the number of people that speak that language. Here we extend the aforementioned model to examine the role of saturation of the fitness on the language dynamics. We found that the dependence of the linguistic diversity on the area after colonization displays a power law regime with a nontrivial exponent in very good agreement with the measured exponent associated with the actual distribution of languages on the Earth.Comment: 10 pages, 3 figure

Human toxocariasis: contribution by Brazilian researchers

In the present paper the main aspects of the natural history of human infection by Toxocara larvae that occasionally result in the occurrence of visceral and/or ocular larva migrans syndrome were reviewed. The contribution by Brazilian researchers was emphasized, especially the staff of the Tropical Medicine Institute of São Paulo (IMT)

Capim-elefante (Pennisetum purpureum Schum.), sob duas doses de nitrogênio. Consumo e produção de leite

A produção de leite, o consumo voluntário de matéria seca e a taxa de passagem da FDN no trato gastrintestinal em quatro épocas do ano (julho, outubro, janeiro e março), de vacas mestiças, sob pastejo de capim-elefante, submetido a dois níveis de nitrogênio (300 e 700 kg de N/ha•ano), foram estimados. Foi usado pastejo rotativo com três dias de ocupação e 30 de descanso, empregando-se 36 vacas lactantes mestiças Holandês x Zebu, em uma lotação de 6 vacas/ha. A estimativa de consumo e a taxa de passagem foram determinadas em apenas 24 animais. Para a coleta de extrusas, foram usadas duas vacas esôfago-fistuladas. O consumo foi estimado usando-se a relação produção fecal:indigestibilidade dos alimentos. A produção fecal foi estimada usando-se a FDN da extrusa marcada com dicromato de sódio fornecida em dose única. O consumo de MS total não foi influenciado pelas doses de N e por épocas do ano, com valores médios diários de 10,9 e 10,5 kg /MS•vaca para as doses de 300 e 700 kg N/ha•ano, respectivamente. Entretanto, o consumo de MS do capim-elefante foi influenciado por doses de N e épocas, com valores de 6,5 e 5,6 kg/vaca•dia para as doses de N supracitadas, respectivamente. O capim-elefante contribuiu com 26,0% da MS total ingerida, durante a época seca (julho/outubro) e sua contribuição aumentou para 84,0% na época das águas (janeiro/março). A produção diária de leite não foi influenciada por doses de N, com produções médias de 11,6 e 12,3 kg de leite/vaca•dia, para as doses de 300 e 700 kg N/ha•ano, respectivamente.The milk production, the dry matter intake (DMI) and the NDF passage rate in the gastrointestinal tract in four seasons (July, October, January and March), of crossbred cows, under grazing of elephant grass, submitted to two levels of nitrogen (300 and 700 kg N/ha•year) were estimated. The rotation grazing with three days of occupation and 30 days of resting, using 36 crossbred Holstein Zebu milking cows, in a stocking rate of 6 cows/ha, was used. The estimates of the intake and passage rate were determined only in 24 animals. For the extrusa collection, two esophageal fistulated cows were used. The intake was estimated using the fecal output: feed indigestibility ratio. The fecal output was estimated using the NDF of the extrusa marked with sodium dichromium fed in an unique dose. The total dry matter intake was not affected by the N levels and the year seasons, with average values of 10.9 and 10.5 kg DM/cow, for the levels of 300 and 700kg N/ha•year, respectively. However, the DM intake of the elephant grass was affected by N levels and year seasons, with reported values of 6.55 e 5.63 kg/cow•day, for the above described N levels, respectively. The elephant grass contributed with 26% of the total DM intake during the dry season (July/October) and its contribution increased up to 84% in the rainy season (January/March). The milk production was not affected by N levels, with reported average production of 11.6 and 12.3 kg/cow•day, for the levels of 300 and 700 kg N/ha•year, respectively

Biochemical and bioactive phytonutrients changes in tissues of two cultivars of fresh-cut cassava in stick form under refrigerated storage

The aim of this study was to evaluate the effects of fresh-cut in stick form of two cassava cultivars on their biochemical and antioxidant characteristics, at two harvest times. The fresh cut cassava sticks were packaged in polypropylene, maintained at 5±1ºC, with 90±5% relative humidity for 12 days. The concentration of carotenoids, total soluble phenolic compounds and the activity of phenylalanine ammonia lyase and antioxidant capacity were significantly higher for the sticks of cultivar 'Amarela' cassava than for the sticks of cultivar 'Cacau'. The concentrations of carotenoids, total soluble phenolic compounds, and the activity of phenylalanine ammonia lyase and antioxidant capacity were all significantly greater when harvested at 14-month of age. There was a significant increase in the activity of phenylalanine ammonia lyase during the 12 days of storage at 5ºC. However, there was a decrease in total carotenoids, soluble phenolic compounds, and in the antioxidant capacity of the two cultivars

Residual biomass potential of commercial and pre-commercial sugarcane cultivars

Sugarcane (Saccharum spp.) is an efficient and sustainable alternative for energy generation compared to non-renewable sources. Currently, during the mechanized harvest process, the straw left in the field can be used in part for the second generation ethanol and increasing the electric energy production. Thus, this study aimed to provide information on the potential for residual biomass cultivars of sugarcane cropping system. This study provides the following information: yield of straw, depending on the calculated leaf area index and the number of tillers per linear meter; primary energy production of several sugarcane genotypes; contribution of dry tops and leaves; biomass yield; and evaluation of fiber, cellulose, hemicellulose and lignin. Preliminary results obtained by researchers of the State of São Paulo, Brazil, and reCviews related studies are presented. The results suggest that the production of sugarcane straw content varies according to the cultivars; the greater mass of sugarcane straw is in the top leaves and that the potential for the crude energy production of sugarcane per area unit can be increased using fiber-rich species or species that produce more straw. The straw indexes was shown to be a good indicator and allow the estimation of straw volumes generated in a sugarcane crop. The cellulose, hemicellulose and lignin composition in sugarcane is distinct among varieties. Therefore, it is possible to develop distinct biomass materials for energy production and for the development of sugarcane mills using biochemical processes and thermal routes