Investigación de salmonella en productos de origen cárnico

A survey was carried out to detect Salmonella in raw, cooked and salted samples from 99 sausages processing plant. Out of 836 samples, 39 (4.66%) Salmonella strains belonging to ten serotypes were isolated and S.anatum accounted for the highest percentage. Out of 324 raw sample products, 33 (9.24%) were most often Salmonella contaminated. It is concluded that the potential for bacterial pathogen contamination of the meat ingredients during manufacture and processing has important epidemiologic implications.Se llevó a cabo un estudio para descubrir Salmonella en muestras crudas, cocidas y salazones de 99 plantas procesadoras de embutidos. Sobre un total de 836 muestras, 39 (4,66%) se aislaron cepas de Salmonella pertenecietes a diez serotipos y S.anatum lo fue en el porcentaje más alto. De 324 productos las muestras crudas, 33 (9.24%) fueron las más contaminadas con Salmonella. Se concluye que el potencial para la contaminación de la bacteriana patógena de los ingredientes de carne durante la fabricación y procesando tiene implicaciones epidemiológicas importantes.Facultad de Ciencias Veterinaria

Chronic Trypanosoma cruzi infection potentiates adipose tissue macrophage polarization toward an anti-inflammatory M2 phenotype and contributes to diabetes progression in a diet-induced obesity model

Chronic obesity and Chagas disease (caused by the protozoan Trypanosoma cruzi) represent serious public health concerns. The interrelation between parasite infection, adipose tissue, immune system and metabolism in an obesogenic context, has not been entirely explored. A novel diet-induced obesity model (DIO) was developed in C57BL/6 wild type mice to examine the effect of chronic infection (DIO+I) on metabolic parameters and on obesity-related disorders. Dyslipidemia, hyperleptinemia, and cardiac/hepatic steatosis were strongly developed in DIO mice. Strikingly, although these metabolic alterations were collectively improved by infection, plasmatic apoB100 levels remain significantly increased in DIO+I, suggesting the presence of pro-atherogenic small and dense LDL particles. Moreover, acute insulin resistance followed by chronic hyperglycemia with hypoinsulinemia was found, evidencing an infection-related-diabetes progression. These lipid and glucose metabolic changes seemed to be highly dependent on TLR4 expression since TLR4-/- mice were protected from obesity and its complications. Notably, chronic infection promoted a strong increase in MCP-1 producing macrophages with a M2 (F4/80+CD11c-CD206+) phenotype associated to oxidative stress in visceral adipose tissue of DIO+I mice. Importantly, infection reduced lipid content but intensified inflammatory infiltrates in target tissues. Thus, parasite persistence in an obesogenic environment and the resulting host immunometabolic dysregulation may contribute to diabetes/atherosclerosis progression.Fil: Cabalén, María Eugenia. Universidad Católica de Córdoba; ArgentinaFil: Cabral, Maria Fernanda. Universidad Católica de Córdoba; ArgentinaFil: Sanmarco, Liliana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Andrada, Marta Cecilia. Universidad Católica de Córdoba; ArgentinaFil: Onofrio, Luisina Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Ponce, Nicolás Eric. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Aoki, Maria del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Gea, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Cano, Roxana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentin

Aislamiento de <i>Brucella canis</i> de leche de hembra canina infectada crónicamente

La transmisión de la brucelosis puede realizarse mediante el contacto directo por vía sexual, por vía oral, nasal o conjuntival, dado que la bacteria se encuentra tanto en el semen y las secreciones vaginales, como en la orina, leche y fetos abortados de los animales enfermos. El carácter crónico de la infección y la posibilidad posterior de concebir, gestar y parir cachorros vivos, facilitan la perpetuidad y difusión de la infección. Informamos aquí el hallazgo bacteriológico de B. canis en sangre y leche de una hembra infectada crónicamente.Facultad de Ciencias Veterinaria

Neoadjuvant gastric cancer treatment and associated nutritional critical domains for the optimization of care pathways: a systematic review

(1) Background: Gastric cancer patients are known to be at a high risk of malnutrition, sarcopenia, and cachexia, and the latter impairs the patient’s nutritional status during their clinical course and also treatment response. A clearer identification of nutrition-related critical points during neoadjuvant treatment for gastric cancer is relevant to managing patient care and predicting clinical outcomes. The aim of this systematic review was to identify and describe nutrition-related critical domains associated with clinical outcomes. (2) Methods: We performed a systematic review (PROSPERO ID:CRD42021266760); (3) Results: This review included 14 studies compiled into three critical domains: patient-related, clinical-related (disease and treatment), and healthcare-related. Body composition changes during neoadjuvant chemotherapy (NAC) accounted for the early termination of chemotherapy and reduced overall survival. Sarcopenia was confirmed to have an independent prognostic value. The role of nutritional interventions during NAC has not been fully explored. (4) Conclusions: Understanding critical domain exposures affecting nutritional status will enable better clinical approaches to optimize care plans. It may also provide an opportunity for the mitigation of poor nutritional status and sarcopenia and their deleterious clinical consequences.info:eu-repo/semantics/publishedVersio

Brucelosis en caninos domésticos: diagnóstico molecular

Las especies que conforman el género Brucella causan enfermedades en animales domésticos y salvajes incluyendo bovinos, ovinos, caprinos, suinos, caninos. La importancia del canino como trasmisor de diversas variedades del género al hombre y demás animales convivientes adquiere una importancia tanto en el ámbito urbano como rural, tanto desde la mirada de la salud pública como desde el aspecto productivo-sanitario en explotaciones comerciales. El objetivo del trabajo es el de presentar los resultados de una prueba de reacción en cadena de la Polimerasa de tipo Multiplex para la identificación de diferentes biovariedades del género Brucella.spp.Facultad de Ciencias Veterinaria

La transmisión por vía de las mucosas en la brucelosis canina

La transmisión por vía de las mucosas en la brucelosis canina causada por Brucella canis ha sido reportada en la bibliografía por numeroso autores. El objetivo del trabajo es presentar un caso de transmisión por vía oral y valorar serológica y microbiológicamente la efectividad de la misma.Facultad de Ciencias Veterinaria

Aislamiento de Brucella melitensis variadad canis ST18 y ST 21 en Argentina

La brucelosis es una enfermedad causada por Brucella canis, que es una bacteria que se aisló por primera vez de los fetos de cachorros muertos en el medio de los años 1960. Se trata de las bacterias más comunes que pueden infectar a las perras. Parece que en los últimos años se ha escrito mucho sobre la brucelosis en la cría de perros, pero a pesar de todo, las tasas de infección puede llegar a alcanzar hasta un 8-10%. Brucella canis también representa un riesgo significativo para la salud pública, ya que es transmisible a los seres humanos. Los seres humanos pueden desarrollar una insuficiencia hepática grave o la artritis.Facultad de Ciencias Veterinaria

Gestão da Qualidade: uma experiência de Implementação da CAF

No contexto actual do processo de Modernização da Administração Pública e do recentemente aprovado Sistema Integrado da Avaliação de Desempenho da Administração Pública (SIADAP), está previsto a avaliação das organizações, a qual deve ser feita através do recurso à Auto-Avaliação. Através da aplicação da CAF - Common Assessment Framework, um modelo de auto-avaliação criado no âmbito da União Europeia especificamente para a Administração Pública, os organismos promovem a gestão da qualidade através da realização de diagnósticos que têm por referência um conjunto de boas práticas e indicadores que caracterizam o desempenho da sua organização. A CAF é uma ferramenta de auto-avaliação. Comparativamente com a EFQM, e embora derivado deste, é um modelo mais simples e mais fácil de aplicar à Administração Pública. Para além de permitir o desenvolvimento de uma cultura de gestão estratégica e de serviço público orientado para o cidadão, permite também o desenvolvimento de práticas de benchmarking, proporcionando assim a tão importante comparabilidade institucional. A Auto-Avaliação, com base na CAF, permite proceder a um diagnóstico do funcionamento e dos resultados da organização.Pretendemos dar um testemunho do percurso da equipa que, desde o inicio, acreditou no Projecto e que acredita numa Cultura de Qualidade aplicada ao sector público. Para que a implementação da CAF na Biblioteca Pública e Arquivo Regional de Ponta Delgada (adiante designada de BPARPD) fosse uma realidade foi necessário um forte empenho da Equipa de Auto-Avaliação que efectuou o diagnóstico de todos os critérios inerentes ao modelo dando cumprimento ao cronograma pré-estabelecido. Mais do que falar do modelo propriamente dito pretende-se partilhar a metodologia utilizada para implementação da CAF, o processo de aprendizagem verificado e os resultados da organização. Ao decidir implementá-la, e tendo em conta a missão e os objectivos da organização, pretendeu-se que o processo fosse uma “preocupação de TODOS” tendo sempre em atenção os pontos fortes e quais os pontos a melhorar

IL-31 and IL-8 in Cutaneous T-Cell Lymphoma: Looking for Their Role in Itch

The itch associated with cutaneous T-cell lymphoma (CTCL), including Mycosis Fungoides (MF) and Sézary syndrome (SS), is often severe and poorly responsive to treatment with antihistamines. Recent studies have highlighted the possible role of interleukins in nonhistaminergic itch. We investigated the role of IL-31 and IL-8 in CTCL, concerning disease severity and associated itch. Serum samples of 27 patients with CTCL (17 MF and 10 SS) and 29 controls (blood donors) were analyzed for interleukin- (IL-) 31 and IL-8; correlations with disease and itch severity were evaluated. IL-31 serum levels were higher in CTCL patients than in controls and higher in SS than in MF. Also, serum IL-31 levels were higher in patients with advanced disease compared to those with early disease, and they correlated positively with lactate dehydrogenase and beta 2-microglobulin levels, as well as with the Sézary cell count. Itch affected 67% of CTCL patients (MF: 47%; SS: 100%). Serum IL-31 levels were higher in itching patients than in controls and in patients without itching. There was no association between serum IL-8 and disease severity, nor with itching. Serum IL-8 levels correlated positively with peripheral blood leukocyte and neutrophil counts in CTCL patients. Our study suggests a role for IL-31 in CTCL-associated itch, especially in advanced disease and SS, offering a rational target for new therapeutic approaches. Increased serum IL-8 observed in some patients may be related to concomitant infections, and its role in exacerbating itch by recruiting neutrophils and promoting the release of neutrophil proteases deserves further investigationinfo:eu-repo/semantics/publishedVersio

Characterization of Novel Pathogenic Variants Leading to Caspase-8 Cleavage-Resistant RIPK1-Induced Autoinflammatory Syndrome

Pathogenic RIPK1 variants have been described as the cause of two different inborn errors of immunity. Biallelic loss-of-function variants cause the recessively inherited RIPK1 deficiency, while monoallelic variants impairing the caspase-8-mediated RIPK1 cleavage provoke a novel autoinflammatory disease (AID) called cleavage-resistant RIPK1-induced autoinflammatory (CRIA) syndrome. The aim of this study was to characterize the pathogenicity of two novel RIPK1 variants located at the cleavage site of caspase-8 detected in patients with dominantly-inherited, early-onset undefined AID. RIPK1 genotyping was performed by Sanger and next-generation sequencing. Clinical and analytical data were collected from medical charts, and in silico and in vitro assays were performed to evaluate the functional consequences. Genetic analyses identified two novel heterozygous RIPK1 variants at the caspase-8 cleavage site (p.Leu321Arg and p.Asp324Gly), which displayed a perfect intrafamilial phenotype-genotype segregation following a dominant inheritance pattern. Structural analyses suggested that these variants disrupt the normal RIPK1 structure, probably making it less accessible to and/or less cleavable by caspase-8. In vitro experiments confirmed that the p.Leu321Arg and p.Asp324Gly RIPK1 variants were resistant to caspase-8-mediated cleavage and induced a constitutive activation of necroptotic pathway in a similar manner that previously characterized RIPK1 variants causing CRIA syndrome. All these results strongly supported the pathogenicity of the two novel RIPK1 variants and the diagnosis of CRIA syndrome in all enrolled patients. Moreover, the evidences here collected expand the phenotypic and genetic diversity of this recently described AID, and provide interesting data about effectiveness of treatments that may benefit future patients