Brugia malayi microfilariae adhere to human vascular endothelial cells in a C3-dependent manner

Brugia malayi causes the human tropical disease, lymphatic filariasis. Microfilariae (Mf) of this nematode live in the bloodstream and are ingested by a feeding mosquito vector. Interestingly, in a remarkable co-evolutionary adaptation, Mf appearance in the peripheral blood follows a circadian periodicity and reaches a peak when the mosquito is most likely to feed. For the remaining hours, the majority of Mf sequester in the lung capillaries. This circadian phenomenon has been widely reported and is likely to maximise parasite fitness and optimise transmission potential. However, the mechanism of Mf sequestration in the lungs remains largely unresolved. In this study, we demonstrate that B. malayi Mf can, directly adhere to vascular endothelial cells under static conditions and under flow conditions, they can bind at high (but not low) flow rates. High flow rates are more likely to be experienced diurnally. Furthermore, a non-periodic nematode adheres less efficiently to endothelial cells. Strikingly C3, the central component of complement, plays a crucial role in the adherence interaction. These novel results show that microfilariae have the ability to bind to endothelial cells, which may explain their sequestration in the lungs, and this binding is increased in the presence of inflammatory mediators

Engaging (Past) Participants: The Case of radicalprintshops.org

Wikis have for some time been heralded for their democratic and participatory potential and there has been significant research into the use of wikis in a variety of contexts. Within academic research they tend to be used by closed groups to manage material rather than for research per se. This chapter describes an experiment and the challenges to do the latter through the instigation of the open access wiki radicalprintshops.org

Rural Transformation of a Village in Telangana, A Study of Dokur since 1970s

The structural changes taking place in villages are partly due to market forces and also because of public policy. The article examines the transformation and development of a village namely Dokur in Telangana, India which has undergone changes since mid-1970s. The village was initially studied in 1975–1984 by the International Crops Research Institute for Semi-Arid Tropics (ICRISAT), but resurveyed from 2001 to 2014. There has been seen a significant change since 1980s in its livelihood diversity. Until the mid-1970s, there was more focus on green revolution technologies under the assumption that the trickle-down effect would take care of poverty. Hence, in the initial years, very few development programmes existed and were mostly focused on agriculture growth. Although public distribution system was in place from the mid 1970s, a new government initiative targeted poverty directly through a 20-point plan. From the 1990s, more specific schemes were introduced, which often targeted poor, scheduled castes and tribes (SC and ST) and other backward castes (OBC) as well as small and marginal farmers also. After realizing that most of the benefits were captured by village elites and large farmers, the focus shifted to self-targeting of various developments and social safety net programmes targeting lower castes and poorer households in the late 2000s, especially after the introduction of Mahatma Gandhi National Rural Employment Guarantee Act (MGNREGA). This was also an attempt to reduce gender bias in the programmes. The participation of poor, SC and ST and women increased after the self-targeting schemes were introduced in the country. The Public Distribution System (PDS), Indira Awas Yojana (IAY), pension schemes, complete sanitation programmes, agricultural input subsidy programme, million wells programmes, loan waiver scheme and the drought relief programmes had positive impacts on livelihoods, but with less targeting. Most of the gains from agricultural subsidies were enjoyed by medium and large farmers, although small SC and ST farmers benefited some what. However, all indicators show a systematic and considerable increase in living standards