Morphometric and phytochemical characterization and elevation effect on yield of three potato landraces of the Ligurian Apennines (Northern Italy)

The great adaptability, productivity and importance in human diet conditioned the diffusion of potato (Solanum tuberosum L.) in very different areas, leading to the formation of numerous landraces through anthropic or natural selection. Genoa mountainous inland is an historical area for potato cultivation and landraces are preserved by farmers associations as “Consorzio della Quarantina”. The aim of this study was the phytochemical and morphometric characterization of three potato landraces of the consortium (Quarantina Bianca, Quarantina Prugnona and Rubra spes) analysing the bio-agronomical performance at different elevations. The commercial cultivar Kennebec was used as control. For the morphometric analysis, the results of pairwise MANOVA shows that only Quarantina Bianca does not present significant differences with Kennebec in the mean shape. The four potato varieties resulted significantly different for the most of the considered phytochemical aspects (among content in ash, starch, solanine, total flavonoids, total phenols, chlorogenic acid, caffeic acid, ferulic acid and radical scavenging activity only starch and solanine content didn’t result statistically different). A remarkable result was a more consistent yield for all the varieties with the increase of elevation in the agronomical trials. We can conclude that Genovese landraces are a good choice to exploit Genovese mountain marginal territories

How harvest, cleaning and conservation good practices affect the quality of saffron: results of a research conducted in Italy

Saffron (Crocus sativus L.) spice making requires time-spending manual operations: stigmas are separated from the flower picked in bud early in the morning, and once dried they are preserved protected from light. This study verified how the correct pursuing of these good practices affects saffron quality. Few hours of exposure of the flower to the sun determined a significant decrease in the colouring strength (239.66 ± 10.33 versus 255.35 ± 11.87). The correct cleaning of stigmas determined a very significant increase of colouring strength (247.12 ± 13.32 instead of 224.35 ± 14.88) and a significant increase of flavour strength (99.72 ±7.48 against 90.31 ± 6.32, p <0.05). In 24 months, all the samples kept in the dark were still of first category of quality while the ones kept in the light dropped in second category. For all samples there was an increase of aroma strength and a decrease of the flavour and colouring strength, but only the colouring strength loss followed a significantly more sloping trendline. A detectable difference in the content of trans-crocin 4 correlated to the ageing or the way of conservation was not found, nevertheless, it was confirmed that the isomers tend towards a photostationary state where the trans isomer is more present

Quality Traits of "Cannabidiol Oils": Cannabinoids Content, Terpene Fingerprint and Oxidation Stability of European Commercially Available Preparations

Cannabidiol (CBD)-based oil preparations are becoming extremely popular, as CBD has been shown to have beneficial effects on human health. CBD-based oil preparations are not unambiguously regulated under the European legislation, as CBD is not considered as a controlled substance. This means that companies can produce and distribute CBD products derived from non-psychoactive hemp varieties, providing an easy access to this extremely advantageous cannabinoid. This leaves consumers with no legal quality guarantees. The objective of this project was to assess the quality of 14 CBD oils commercially available in European countries. An in-depth chemical profiling of cannabinoids, terpenes and oxidation products was conducted by means of GC-MS and HPLC-Q-Exactive-Orbitrap-MS in order to improve knowledge regarding the characteristics of CBD oils. Nine out of the 14 samples studied had concentrations that differed notably from the declared amount, while the remaining five preserved CBD within optimal limits. Our results highlighted a wide variability in cannabinoids profile that justifies the need for strict and standardized regulations. In addition, the terpenes fingerprint may serve as an indicator of the quality of hemp varieties, while the lipid oxidation products profile could contribute in evaluation of the stability of the oil used as milieu for CBD rich extracts

Exploring the stabilizing effect on the i-motif of neighboring structural motifs and drugs

Cytosine-rich DNA sequences may fold into a structure known as i-motif, with potential in vivo modulation of gene expression. The stability of the i-motif is residual at neutral pH values. To increase it, the addition of neighboring moieties, such as Watson-Crick stabilized loops, tetrads, or non-canonical base pairs have been proposed. Taking a recently described i-motif structure as a model, the relative effect of these structural moieties, as well as several DNA ligands, on the stabilization of the i-motif has been studied. To this end, not only the original sequence but different mutants were considered. Spectroscopic techniques, PAGE, and multivariate data analysis methods have been used to model the folding/unfolding equilibria induced by changes of pH, temperature, and the presence of ligands. The results have shown that the duplex is the moiety that is responsible of the stabilization of the i-motif structure at neutral pH. The T:T base pair, on the contrary, shows little stabilization of the i-motif. From several selected DNA-binding ligands, the G-quadruplex ligand BA41 is shown to interact with the duplex moiety, whereas non-specific interaction and little stabilization has been observed within the i-motif

Alkaloid escholidine and its interaction with DNA structures

Berberine, the most known quaternary protoberberine alkaloid (QPA), has been reported to inhibit the SIK3 protein connected with breast cancer. Berberine also appears to reduce the bcl-2 and XIAP expression-proteins responsible for the inhibition of apoptosis. As some problems in the therapy with berberine arose, we studied the DNA binding properties of escholidine, another QPA alkaloid. CD, fluorescence, and NMR examined models of i-motif and G-quadruplex sequences present in the n-myc gene and the c-kit gene. We provide evidence that escholidine does not induce stabilization of the i-motif sequences, while the interaction with G-quadruplex structures appears to be more significant

Antifungal activity and biochemical profiling of exudates from germinating maize Nostrano di Storo local variety

Plant pathogens are responsible for important damages to valuable crops causing important economic losses. Agrobiodiversity protection is crucial for the valorization of local varieties that could possess higher resistance to biotic and abiotic stress. At the beginning of germination, seeds are susceptible to pathogens attacks, thus they can release endogenous antimicrobial compounds of different natures in the spermosphere, to contrast proliferation of microorganisms. The work aimed at characterizing the maize of local variety Nostrano di Storo seed exudates secreted during the first phases of germination, to identify compounds active in the defense towards pathogens. Storo seed exudates were proven to inhibit F. verticilloides germination. In order to investigate the cause of the described effect, compositional profiling of the exudates was performed through NMR, lipidomic, and proteomic analyses. This study suggests an important role of microbial endophytic communities in the protection of the seed during the early phases of the germination process and their interplay with fatty acids released by the seeds, rather than a specific antifungal compound. The valorization of agronomically acceptable maize lines with pre-harvest enhanced resistances to pathogens contamination could lead, in the near future, to commercially available varieties potentially requiring more limited chemical protective treatments

Antiviral, virucidal and antioxidant properties of artemisia annua against {SARS}-{CoV}-2

Natural products are a rich source of bioactive molecules that have potential pharmacotherapeutic applications. In this study, we focused on Artemisia annua (A. annua) and its enriched extracts which were biologically evaluated in vitro as virucidal, antiviral, and antioxidant agents, with a potential application against the COVID-19 infection. The crude extract showed virucidal, antiviral and antioxidant effects in concentrations that did not affect cell viability. Scopoletin, arteannuin B and artemisinic acid (single fractions isolated from A. annua) exerted a considerable virucidal and antiviral effect in vitro starting from a concentration of 50 μg/mL. Data from Surface Plasmon Resonance (SPR) showed that the inhibition of the viral infection was due to the interaction of these compounds with the 3CLpro and Spike proteins of SARS-CoV-2, suggesting that the main interaction of compounds may interfere with the viral pathways during the insertion and the replication process. The present study suggests that natural extract of A. annua and its components could have a key role as antioxidants and antiviral agents and support the fight against SARS-CoV-2 variants and other possible emerging Coronaviruses

Libro "In cucina con l'erisimo"

Libro di ricette con l'erba dei cantanti, prodotto all'interno del progetto Erisimo a Milan

The Effect of Drought on <i>Sisymbrium officinale</i> (L.) Wild Species for Potential Cultivation as a Leafy Vegetable

Leafy vegetables are common components of the human diet and are a source of antioxidant, vitamins, minerals, and bioactive compounds. Fresh-cut or minimally processed industries are always looking for product innovations. Many wild species, based on their composition, can be evaluated as potential vegetables. In this work, hedge mustard has been studied as a potential leafy vegetable, and two wild populations were grown under 100% crop water requirement (WR) and 50% WR. The effect of water reduction was monitored using non-destructive measurements of chlorophyll a fluorescence and by the analytical determination of primary or secondary metabolism associated parameters such as sugars, anthocyanins, carotenoids, phenolic compounds, and nitrate concentrations. The results demonstrated that hedge mustard [Sisymbrium officinale (L.) Scop.] can be grown with 50% WR without yield reduction. The yield was not statistically different between the two water regimes and ranged from 22.3 to 40 g plant−1 FW. Leaf nitrate concentrations showed high variability in the MI population grown with 100% WR, while in the BG population, they did not change when the WR was shifted from 100% to 50%. The total phenols were 25% higher in the leaves of plants grown under 50% WR in both wild populations. The total sugars and anthocyanins did not show significant variations. Chlorophyll a fluorescence parameters did not show significant changes. The results suggest that hedge mustard can be grown in environments with limited water availability or in the winter season using less water to avoid disease development. The highest yield was obtained from the BG population