Deliverable D9.11: Interim Dissemination and Exploitation - List of events dedicated to the dissemination and exploitation - Dissemination kit

The objective of the Dissemination and Exploitation plan is to identify and organise the activities that are aimed to disseminate the project’s results and to promote their industrial exploitation. The plan is expanded into two directions: i) towards the notification of the project results to the scientific community and citizens (especially school teachers and students); ii) towards communication with farmers and agrochemical companies to exploit the industrial potential of the NoPEST findings. The objectives of the plan will be reached by transferring the experimental ideas and the gained knowledge through multiple tools and activities that include a dedicated website, attendance of several international conferences, publications in open-access peer-reviewed journals and presentations of data to selected exhibitions dedicated to technologies and solution for agricultur

Constructed Wetlands

These file report information about bacteria isolation and identification from Phragmites plants collected from a Constructed Wetlands in Morocco. The bacteria were characterized for the potential capacity to promote plant growth in vitro and in vivo on model plants. Moreover, their ability to degrade and/or tolerate different classes of pollutants was assessed. More information on the conditions applied for the plant growth and the overall experimental plan are included in the manuscript Riva et al. (submitted)

Deliverable D1.1: List of candidate cell wall target enzymes - M6 (P1 and P2)

List of candidate cell wall target enzymes to be used for the Yeast two-hybrid screening, as indicated in Deliverable D1.1 due at Month 6

Deliverable D2.1: Linear and cyclic peptide aptamer libraries

Description of the strategies adopted to generate the linear and circular peptide aptamer libraries, according to what described in Task 2.1 Task 2.1: Yeast two-hybrid libraries. Two peptide aptamer yeast libraries will be generated. The first will be made of 24 bp random nucleotide fragments, encoding 8 aa linear peptide aptamers, introduced into the constrained, surfaced-exposed loop within the active center of the thioredoxin A (TrxA) protein and fused to the activation domain of the yeast transcription factor GAL4 (Clontech). A second library will be made of 8 aa cyclic peptides also fused to the activation domain of GAL4. This strategy is based on the intein-mediated lactam peptide reaction to generate a “lariat peptide”. Short linear and cyclic peptides are desirable since they are stable, can be synthesized using solid-phase chemistry, and can cross cell membranes, easily, either alone or in combination with Cell Penetrating Peptides (CPPs)

PGP test on Mangrove

This file reports information about the data included in the file Excel 'RAW DATA PGP TESTS'. More information on the conditions applied for the plant growth and the overall experimental plan are included in the manuscript Soldan et al. (submitted)

Tables S1_Tartarotti et al. submitted to TECTONICS

Table S1. Sample list with geographic location and mineral parageneses (Mineral Abbreviation according to Siivola & Schmid, 2007). Omp(c): coarse-grained omphacite; Omp(f): fine-grained omphacite. Mineral abbrevtiation after Siivola and Schmid (2007)

16S rRNA gene sequencing data

This dataset contains the following files:\r\n\r\n(1) 20 files \"fastq.gz\": files containing the raw sequencing data (FASTQ files) of the 16S rRNA gene profiling carried out on DNA extracted from BAL of antibiotic-treated (files from A1 to A5) and untreated mice (files from C1 to C5). The 16S rRNA gene was amplified using primers EUBF 5’-TCCTACGGGAGGCAGCAGT-3’ and EUBR 5’ -GGACTACCAGGGTATCTAATCCTGTT-3’ (DOI: 10.1099/00221287-148-1-257), which target the V3-V4 hypervariable regions, and sequenced using MiSeq Illumina® technology (2 x 300 paired-end MiSeq kit V3, set to encompass 467-bp amplicon) as previously described (DOI: 10.1371/journal.pone.0142334; DOI: 10.1111/trf.13477).\r\n\r\n(2) File named \"16S rRNA gene sequences.docx\": Word file containing the nucleotide sequences of part of the 16S rRNA gene amplified from the DNA of the bacterial strains isolated from the bronchoalveolar lavage fluid (BAL) of antibiotic-treated and untreated mice. Before each sequence, the first output of BLASTN search performed with default parameters against the \"16 ribosomal RNA sequences (Bacteria and Archaea)\" database.\r\n\r\nFor any additional need to this datasets, contact: [email protected] and [email protected]

Technical Report First Year

Summary of the data related to the research activities performed within the first year of the projec

Blood Microbiomics data at baseline

16S rRNA gene profiling data of DNA extracted from 50 blood samples collected from heatlhy elederly people.\r\nBacterial populations contained in the samples were determined using next generation high throughput sequencing of variable regions (V3-V4) of the 16S rRNA bacterial gene through MiSeq Illumina technology

PGP test on Citrus

These file report information about bacteria isolation and identification from Citrus plants and their potential to promote plant growth in vitro and in vivo on model plants. More information on the conditions applied for the plant growth and the overall experimental plan are included in the manuscript Cherni et al. (submitted). This data have been published in Applied Soil Ecology (Volume 135, March 2019, Pages 182-193; https://doi.org/10.1016/j.apsoil.2018.12.006)