An Intensive Pedestrian Survey For A 143-Acre Development And Outfall Project Near Pederson Road And Williow Fork In Waller And Fort Bend Counties, Texas

HRA Gray & Pape, LLC., of Houston, Texas, conducted an intensive pedestrian cultural resources survey of approximately 58 hectares (143 acres) of property proposed for development as well as 2 outfalls in Waller and Fort Bend Counties, Texas. The United States Army Corps of Engineers has been identified as the Lead Agency for this Project. The goals of the survey were to determine if the Project would affect any previously identified archaeological sites as defined by Section 106 of the National Historic Preservation Act of 1966, as amended (36 CFR 800), and to establish whether or not previously unidentified buried archaeological resources were located within the Project’s Area of Potential Effects. The procedures to be followed by the United States Army Corps of Engineers to fulfill the requirements set forth in the National Historic Preservation Act, other applicable historic preservation laws, and Presidential directives as they relate to the regulatory program of the United States Army Corps of Engineers (33 CFR Parts 320-334) are articulated in the Regulatory Program of the United States Army Corps of Engineers, Part 325 - Processing of Department of the Army Permits, Appendix C - Procedures for the Protection of Historic Properties. All fieldwork and reporting activities were completed with reference to state (the Antiquities Code of Texas) and federal (National Historic Preservation Act) guidelines. Survey and site identification followed Texas Antiquities Code standards. The Project area includes private property; therefore, a Texas Antiquities Permit was not required. The property boundary for this Project is approximately 58 hectares (143 acres) and includes 2 outfalls. This defines the Area of Potential Effects. Field investigation consisted of visual inspection, subsurface shovel testing and supplemental deep testing. Subsurface investigation resulted in the excavation of 130 shovel tests and 9 manual bucket auger tests. All were negative for archaeological deposits and no intact structures of historic-age were observed within or immediately adjacent to the Area of Potential Effects. Based on the negative results of the archaeological investigation, HRA Gray & Pape, LLC. recommends no further archaeological work for this Project

A Cultural Resources Survey Of Texas Eastern Transmission LPS Phase 2 And Phase 3 Pipeline Anomaly Repairs On The PETR To TIVO Segment Of Line 16 In Refugio And Aransas Counties, Texas

On behalf of Texas Eastern Transmission, LP and Edge Engineering and Science, LLC., HRA Gray & Pape, LLC, of Houston, Texas, has completed a 100% pedestrian cultural resources survey and limited shovel testing of an estimated 7.84 kilometers (4.87 miles) of linear area in Refugio and Aransas Counties, Texas. Texas Eastern has identified several locations along the PETR to TIVO Segment of Line 16 that require replacement of aging pipe. Two of the areas requiring maintenance work are identified as “Phase 2” and “Phase 3” for which the archaeological survey was completed in May of 2015. The Lead Federal Agency has been identified as the United States Army Corps of Engineers, Galveston District. Thus the goals of the survey were to determine if land altering activities required to complete this project would affect any previously identified historic properties as defined by Section 106 of the National Historic Preservation Act of 1966, as amended (36 CFR 800), and to establish whether or not previously unidentified cultural resources were located within the project’s Area of Potential Effects (Advisory Council for Historic Preservation 2004). Initial investigation consisted of a background literature and site files search to identify the presence of recorded sites in close proximity to the project area. No previous archaeological linear or area surveys have taken place within the project area. No previously recorded archaeological sites were identified within the project area. The project is located on private property, thus an Antiquities Code of Texas Permit was not required prior to performing fieldwork. All work was conducted following accepted standards set forth by the Texas Historical Commission and the Council of Texas Archeologists. All 7.84 kilometers (4.87 miles) of the proposed “Phase 2” and “Phase 3” pipeline replacement areas have been investigated. Fieldwork for the workspaces was completed in a single mobilization that took place from May 22 to May 29, 2015, and required 128 person hours to complete. Field investigation was conducted entirely on privately-owned properties and consisted of walkover and shovel testing within the project area. During this investigation, no cultural resources were identified or confirmed within the project’s Area of Potential Effects. Based on the negative findings of the survey, HRA Gray & Pape, LLC. recommends that no further cultural resources work be required for the “Phase 2” and “Phase 3” portions of the project and that the project be allowed to proceed as planned within the surveyed areas

Archaeological Survey for the State Highway 36 Expansion Project in Austin and Fort Bend Counties, Texas

In June 2015, HRA Gray & Pape, LLC., of Houston, Texas, at the request of HNTB Corporation, conducted intensive pedestrian archaeological investigations within approximately 24 kilometers (14.7 miles) of property proposed for the expansion of the State Highway 36 corridor between Highway 90 and Farm-to-Market Road 1952 in Fort Bend and Austin Counties, Texas. The Texas Department of Transportation has been identified as the Lead Agency for this project. Work for this project will be completed as part of the Texas Department of Transportation Project CSJ Numbers 0187-05-049 and 0187- 04-029 by the Houston and Yoakum Districts. The goals of the survey were to determine if the initiation of this project would affect any previously identified historic properties as defined by Section 106 of the National Historic Preservation Act of 1966, as amended (36 CFR 800), and to establish whether or not previously unidentified archaeological resources were located within the project’s Area of Potential Effects. All fieldwork and reporting activities were completed with reference to state (Antiquities Code of Texas) and federal (National Historic Preservation Act of 1966, as amended [36 CFR 800]) guidelines, and according to standards set forth by the Texas Department of Transportation Programmatic Agreement for Enhancement Projects. All research and reporting for this project was completed with reference to Texas Department of Transportation’s current Standards of Uniformity for Technical Reports with regard to Review Standards for Archaeological Survey and Reporting (Texas Department of Transportation 2011; version 3). The Area of Potential Effects for this project is defined as the project length, the existing and proposed right-of-way, and the depth of construction impacts, composed of a total of 123.5 hectares (305.2 acres). Of this, the existing right-of-way subsumes a total of 107 hectares (264 acres), while new proposed right-of-way will comprise the remaining 16.7 hectares (41.20 acres). The total area to be subjected to archaeological survey within the Area of Potential Effects is 16.7 hectares (41.20 acres), comprised of all property proposed as new right-of-way on the south side of State Highway 36. Of the 16.7 hectares (41.20 acres) of new right-of-way, right-of-entry was not provided for approximately 10.8 hectares (26.8 acres). These parcels were subjected to a desktop assessment. Although the depth of impact is unknown, belowground impacts are expected to be typical of road construction activities, impacting up to 1 meter (3 feet) below natural grade. The Area of Potential Effects depth may extend to culturally sterile depths across the project right-of-way. More often the project will entail the building up of material from the existing ground surface. No deep impacts are anticipated within the project area. The intensive pedestrian and reconnaissance surveys completed for the service areas discussed in this report took place on easements belonging to the Texas Department of Transportation and privately-owned property. Right-of-entry was obtained by the client for privately owned land. Work conducted to complete this survey was conducted under Texas Antiquities Permit number 6609, issued by the Texas Historical Commission’s Division of Archeology in July 2013 No new or previously identified archaeological sites were recorded during the investigation. Testing results displayed soil profiles indicative of those that are mapped for the area and gave no indication of buried cultural horizons within the proposed depth of the Area of Potential Effects. Based on the negative results of the intensive pedestrian survey, HRA Gray & Pape, LLC. recommends no further work and that the project be allowed to proceed as planned

A Cultural Resources Survey Of Approximately 590 Acres For The Fbmud 215 In Fort Bend County, Texas

HRA Gray & Pape, LLC, of Houston, Texas, conducted a cultural resources survey on property proposed for residential development in Fort Bend County, Texas. The United States Army Corps of Engineers has been identified as the Lead Agency for this project. The goals of the survey were to determine if the project would affect any previously identified archaeological sites as defined by Section 106 of the National Historic Preservation Act of 1966, as amended (36 CFR 800), and to establish whether or not previously unidentified buried archaeological resources were located within the project’s Area of Potential Effects. The procedures to be followed by the United States Army Corps of Engineers to fulfill the requirements set forth in the National Historic Preservation Act, other applicable historic preservation laws, and Presidential directives as they relate to the regulatory program of the United States Army Corps of Engineers (33 CFR Parts 320-334) are articulated in the Regulatory Program of the United States Army Corps of Engineers, Part 325 - Processing of Department of the Army Permits, Appendix C - Procedures for the Protection of Historic Properties. All fieldwork and reporting activities were completed with reference to State laws and guidelines (the Antiquities Code of Texas). Survey and site identification followed Texas Antiquities Code standards. No Texas Antiquities Permit was required as all survey work was completed on privately-owned property. The property boundary for this project is approximately 238.8 hectares (590 acres). This defines the Area of Potential Effects. Field investigation consisted of visual inspection, subsurface shovel testing, and supplemental deep testing. Subsurface investigation resulted in the excavation of 206 shovel tests and 5 backhoe trenches. Four cultural resources were identified during survey: a historic-age barn and corral dating to the mid-twentieth century and 2 historic archaeological sites (41FB343 and 41FB344) that are interpreted as being associated with an early twentieth century domestic occupation. HRA Gray & Pape, LLC. recommends that these cultural resources are not eligible for listing on the National Register of Historic Places. Additionally, an attempt was made to relocate 4 historic sites within the project area that were recorded during a previous survey by Prewitt and Associates, Inc. Sites 41FB110, 41FB111, 41FB112 and 41FB113 were not relocated, and land modification may have removed any trace of these light-density sites. A trash pile that may be associated with Site 41FB110 was observed. These sites were recommended as not eligible by Prewitt and Associates, Inc.; HRA Gray & Pape, LLC. collected no additional data that would suggest otherwise. It is the recommendation of HRA Gray & Pape, LLC that use of the project be allowed to proceed as planned

The Adsorption of Atomic Nitrogen on Ru(0001): Geometry and Energetics

The local adsorption geometries of the (2x2)-N and the (sqrt(3)x sqrt(3))R30^o -N phases on the Ru(0001) surface are determined by analyzing low-energy electron diffraction (LEED) intensity data. For both phases, nitrogen occupies the threefold hcp site. The nitrogen sinks deeply into the top Ru layer resulting in a N-Ru interlayer distance of 1.05 AA and 1.10 AA in the (2x2) and the (sqrt(3)x sqrt(3))R30^o unit cell, respectively. This result is attributed to a strong N binding to the Ru surface (Ru--N bond length = 1.93 AA) in both phases as also evidenced by ab-initio calculations which revealed binding energies of 5.82 eV and 5.59 eV, respectively.Comment: 17 pages, 5 figures. Submitted to Chem. Phys. Lett. (October 10, 1996

The AMS-02 Anticoincidence Counter

The AMS-02 detector will measure cosmic rays on the International Space Station. This contribution will cover production, testing, space qualification and integration of the AMS-02 anticoincidence counter. The anticoincidence counter is needed to to assure a clean track reconstruction for the charge determination and to reduce the trigger rate during periods of high flux.Comment: IPRD08 conference proceeding. Will be published in Nuclear Physics B (Proceedings Supplement

A practical guide to interpreting and generating bottom-up proteomics data visualizations

Mass-spectrometry based bottom-up proteomics is the main method to analyze proteomes comprehensively and the rapid evolution of instrumentation and data analysis has made the technology widely available. Data visualization is an integral part of the analysis process and it is crucial for the communication of results. This is a major challenge due to the immense complexity of MS data. In this review, we provide an overview of commonly used visualizations, starting with raw data of traditional and novel MS technologies, then basic peptide and protein level analyses, and finally visualization of highly complex datasets and networks. We specifically provide guidance on how to critically interpret and discuss the multitude of different proteomics data visualizations. Furthermore, we highlight Python-based libraries and other open science tools that can be applied for independent and transparent generation of customized visualizations. To further encourage programmatic data visualization, we provide the Python code used to generate all data figures in this review on GitHub ().DATA AVAILABILITY STATEMENT Proteomics data from the following ProteomeExchange repositories were reused to generate Figures in this study: PXD012867, PXD017703, PXD010697, PXD010103

Lessons from the Classroom – assessing the work of postgraduate students to support better hygrothermal risk assessment

The widespread adoption of transient simulation modelling tools by building design professionals to support hygrothermal risk assessment of building design specifications is a crucial component in a multi-pronged drive to reduce moisture risk in buildings. Structured upskilling is essential. Much can be learnt about the ways practitioners use such tools by reviewing the work of professional postgraduate student groups. Such review could inform the creation of a user protocol. Peer-review under the responsibility of the organizing committee of the ICMB21

A Cultural Resources Survey Of Texas Eastern Transmission LPS Phase 1 Pipeline Anomaly Repairs On The PETR To Tivo Segment Of Line 16 In Refugio And Aransas Counties, Texas

On behalf of Texas Eastern Transmission, LP and Edge Engineering and Science, LLC., HRA Gray & Pape, LLC, of Houston, Texas, has completed a 100% pedestrian cultural resources survey and limited shovel testing of an estimated 4.5 kilometers (2.8 miles) of linear area in Refugio and Aransas Counties, Texas. Texas Eastern has identified several locations along the PETR to TIVO Segment of Line 16 that require replacement of aging pipe. One of the areas requiring maintenance work is identified as “Phase 1”, for which the archaeological survey was completed in May of 2015. The Lead Federal Agency has been identified as the United States Army Corps of Engineers, Galveston District. Thus the goals of the survey were to determine if land altering activities required to complete this project would affect any previously identified historic properties as defined by Section 106 of the National Historic Preservation Act of 1966, as amended (36 CFR 800), and to establish whether or not previously unidentified cultural resources were located within the project’s Area of Potential Effects (Advisory Council for Historic Preservation 2004). Initial investigation consisted of a background literature and site files search to identify the presence of recorded sites in close proximity to the project area. No previous archaeological linear or area surveys have taken place within the project area. No previously recorded archaeological sites were identified within the project area. The project is located on private property, thus an Antiquities Code of Texas Permit was not required prior to performing fieldwork. All work was conducted following accepted standards set forth by the Texas Historical Commission and the Council of Texas Archeologists. All 4.5 kilometers (2.8 miles) of the proposed “Phase 1” pipeline replacement area have been investigated. Fieldwork for the Phase I workspaces was completed in a single mobilization that took place from May 18 to May 22, 2015, and required 96 person hours to complete. Field investigation was conducted entirely on privately owned properties and consisted of walkover and shovel testing within the project area. During this investigation no cultural resources were identified or confirmed within the project’s Area of Potential Effects. Based on the negative findings of the survey, HRA Gray & Pape, LLC. recommends that no further cultural resources work be required for the “Phase 1” portion of the project and that the project be allowed to proceed as planned within the surveyed areas. Additional project phases are in the process of being surveyed. Results of those efforts will be submitted when completed

Investigations on the performance of cell lines with cassette exchange system

Die kommerzielle Entwicklung von Produzentenzelllinien basiert meist auf der zufälligen Integration vieler Transgenkopien in das Wirtsgenom und der anschließenden Selektion zur Identifizierung stabiler Hochproduzenten. Das Rekombinase-vermittelte Kassettenaustauschsystem (RMCE) ist bereits in früheren Arbeiten als aussichtsreiche Alternative für die vorhersagbare Entwicklung von Zelllinien beschrieben worden. Ziel dieser Arbeit war die Untersuchung inwieweit RMCE-basierte Zelllinien im Vergleich zu konventionell entwickelten auch in Kultivierungsprozessen vorhersagbare und homogene Wachstums- und Produktionseigenschaften aufweisen. Aus diesem Grund war zunächst die parallele Entwicklung des RMCE-Systems sowie einer Modellzelllinie erforderlich. Die RMCE-kompatiblen Masterzelllinien wurden basierend auf CHO-Suspensionszellen mit eGFP-transduzierenden Lentiviren markiert. Nach der Identifikation austauschbarer und transkriptionell aktiver chromosomaler Loci wurden RMCE-Versuche mit drei vollständigen Antikörpern, einem Antikörper-Fragment und tPA durchgeführt. Die Selektion von RMCE-Subklonen erfolgte mittels zweifacher FACS-Anreicherung ohne Einsatz selektiver Agentien. Die Subklone wiesen eine homogene Expression auf, welche mit dem jeweiligen Targetingpool korrespondierte. Die Ergebnisse deuten jedoch nicht auf die universelle Einsetzbarkeit eines definierten Locus für die Expression verschiedener Proteine hin. Es scheint vielmehr, dass bestimmte Loci für die Expression bestimmter Proteine geeignet sind. Die GS-basierten Modellzelllinien, welche mittels zweifacher Nukleoporation generiert wurden, erzielten finale Titer von 3,5 g L-1 in Fed-Batch Versuchen. Die untersuchten Klone waren in Abwesenheit von MSX jedoch nicht langzeitstabil. Ferner konnte kein positiver Zusammenhang zwischen der MSX-Konzentration und der spezifischen Produktivität der GS-Klone erkannt werden. Bei den im Versuchsraum untersuchten Einflussfaktoren während der Fed-Batch Kultivierung zeigte sich, dass die drei von einem Transfektionspool stammenden GS6 Klone unterschiedlich signifikant beeinflusst werden. Dies korreliert mit den während der Klonselektion beobachteten heterogenen Produktivitäten der GS-Klone. Die drei RMCE-Klone, welche aus zwei unterschiedlichen Targetingpoolen hervorgegangen sind und zwei verschiedene vollständige Antikörper exprimierten, zeigten in Fed-Batch Kultivierungen innerhalb des Versuchsraumes vergleichbare Wachstums- und Produktionseigenschaften.Conventional cell line development is mostly based on random multicopy transgene integration followed by a time and labor consuming screening procedure to identify stable high-producer. Previous publications characterized the recombinase-mediated cassette exchange (RMCE) as a promising alternative for the development of cell lines in a predictable manner. Aim of this thesis was to investigate whether RMCE-based cell lines reveal comparable and predictable growth and production characteristics in culture processes as well in comparison to conventional cell lines. For this reason the parallel development of the RMCE-system as well as a GS-based model cell lines was required. RMCE master cell lines based on CHO suspension cells were tagged with eGFP transducing lentiviruses. After having identified exchangeable and transcriptionally active chromosomal loci, RMCE experiments with three fullsize antibodies, one antibody fragment and tPA have been performed. The selection of positive RMCE-events was conducted via FACS without any selective agents. The transgene expression among the subclones was homogenous and moreover corresponding to the respective targetingpool. However, the results do not indicate the universal applicability of a certain chromosomal locus for recombinant protein expression. The results rather support the theory that some loci are appropriate for the expression of certain proteins, since the expression level among the full size antibodies was quite similar. The GS-based model cell lines were established after dual nucleofection and subsequent selection by means of a single cell secretion assay. In fed-batch processes the GS-model cell lines reached peak titers of 3.5 g L-1. However, in absence of MSX the clones were not stable. Moreover no positive correlation was observed between MSX-concentration (for gene amplification) and specific productivity of the respective GS-clones. Investigation of GS-clones within a defined design-space in fed-batch processes revealed different significantly influencing parameters among the clones deriving from a common pool. This result correlated with the heterogeneous productivities among the GS-clones during the clonal screening procedure. Three antibody producing RMCE-derived clones have been investigated exemplarily as well in fed-batch processes within a defined design space. All of them demonstrated comparable growth and production characteristics