Comparative Analysis Of Centers For Entrepreneurship At Two Central Florida Universities

Studies have attempted to explain the linkage between achieving success in the field of entrepreneurship and the pedagogy instituted to teach the skills entrepreneurs need to achieve success in their chosen endeavors. It is widely known and well documented that people have experienced entrepreneurial success with limited, and sometimes no formal entrepreneurial training. The ever present question of “can entrepreneurship be taught” has been debated from many varying perspectives. The late Peter Drucker pragmatically once said “The entrepreneur mystique? It’s not magic, it’s not mysterious, and it has nothing to do with the genes. It’s a discipline. And, like any discipline, it can be learned” (Drucker, 1985). A study conducted by the Center for College Affordability and Productivity recently determined that almost half of Americans with college degrees are overqualified for their jobs. Many studies have also concluded that college graduates accumulate greater lifetime earnings than non-college graduates. Yet the escalating costs of attending college and the diminishing prospects of job security after attaining a college degree have brought the cost of education to the precipice of a potential “education bubble”. Student loan debt exceeds One Trillion Dollars and the typical student loan needs to be repaid over ten years at nearly seven percent interest. Similar to the recently experienced “housing bubble” there is a genuine concern, as it relates to education, that today’s populace is paying too much for something that yields limited value. Therefore, the question of “can entrepreneurship be taught” should be supplanted with “can entrepreneurship be learned?” “Are graduates capable of applying their academic training to produce tangible results?” If there are too many academic degrees being generated that are unable to be absorbed into a stagnant job market, it would stand to reason that a college degree, from a business school iv or any co-curricular discipline, without significant concentration in the study of entrepreneurship, serves only a limited purpose in a growing, capitalistic society that is predicated on job growth. Centers for entrepreneurship provide an excellent foundation for invigorating new college graduates from multiple academic disciplines with the motivation and desire to achieve success in business as entrepreneurs. This comparative analysis of two thriving and vibrant Centers for Entrepreneurship at major universities in the growing central Florida region examines their best practices and compares them to current national guidelines established by the Global Consortium of Entrepreneurship Centers, a 200 + member organization domiciled in the Kelley School of Business at Indiana University in Bloomington, Indiana that serves as the key junction for university-based entrepreneurship centers across the United States to collaborate, communicate and jointly advance excellence in entrepreneurship (www.globalentrepreneurshipconsortium.org). The evaluator and author of this dissertation implemented procedures similar to those used in accreditation reviews and applied professional judgment techniques to design a connoisseurship evaluation of entrepreneurship centers at two major universities --- The Center for Entrepreneurship at the University of South Florida in Tampa, FL and The Center for Entrepreneurial Leadership at the University of Central Florida in Orlando, FL. We have all heard the Horatio Alger “rags to riches” stories of entrepreneurs who “bootstrapped” their business ideas without benefit of any formal business or entrepreneurial education. But it is just as great a likelihood in the coming years that we will admire those who give the credit for their success to the concepts they mastered in an entrepreneurial studies program and how their alma maters provided mentors through their centers for entrepreneurship who saved them from committing an abundance of mistakes by trial and error as they transported v their business ideas from conceptualization to realization. This research will assist centers of entrepreneurship as they strive to incorporate standards of excellence to benefit students who endeavor to become business and job creators in the future

GEM: An Interactive Simulation Model of the Global Economy

One of the aims of the International Institute for Applied Systems Analysis (IIASA) is to develop methods of systems analysis that lend themselves to applications to policy analysis. Gaming has proved to be an important methodological planning tool in such areas as military and business affairs, but thus far few applications to socioeconomic planning have been attempted. The game described here is a demonstration model, intended to acquaint the reader with the potentialities of this approach as a preanalytical research tool. The model described here, which is named "GEM" (Global Economic Model), is a six person interactive simulation model (or game) intended to generate intuitive insights into the economic interactions among six world regions over the next 50 years

Outer Membrane Integrity-Dependent Fluorescence of the Japanese Eel UnaG Protein in Live Escherichia coli Cells

Reporter genes are important tools in many biological disciplines. The discovery of novel reporter genes is relatively rare. However, known reporter genes are constantly applied to novel applications. This study reports the performance of the bilirubin-dependent fluorescent protein UnaG from the Japanese eel Anguilla japonicas in live Escherichia coli cells in response to the disruption of outer membrane (OM) integrity at low bilirubin (BR) concentrations. Using the E. coli wild-type strain MC4100, its isogenic OM-deficient mutant strain NR698, and different OM-active compounds, we show that BR uptake and UnaG fluorescence depend on a leaky OM at concentrations of 10 µM BR and below, while fluorescence is mostly OM integrity-independent at concentrations above 50 µM BR. We suggest that these properties of the UnaG–BR couple might be applied as a biosensor as an alternative to the OM integrity assays currently in use

Dual autoencoders modeling of electronic health records for adverse drug event preventability prediction

Background Elderly patients are at increased risk for Adverse Drug Events (ADEs). Proactively screening elderly people visiting the emergency department for the possibility of their hospital admission being drug-related helps to improve patient care as well as prevent potential unnecessary medical costs. Existing routine ADE assessment heavily relies on a rule-based checking process. Recently, machine learning methods have been shown to be effective in automating the detection of ADEs, however, most approaches used only either structured data or free texts for their feature engineering. How to better exploit all available EHRs data for better predictive modeling remains an important question. On the other hand, automated reasoning for the preventability of ADEs is still a nascent line of research. Methods Clinical information of 714 elderly ED-visit patients with ADE preventability labels was provided as ground truth data by Jeroen Bosch Ziekenhuis hospital, the Netherlands. Methods were developed to address the challenges of applying feature engineering to heterogeneous EHRs data. A Dual Autoencoders (2AE) model was proposed to solve the problem of imbalance embedded in the existing training data. Results Experimental results showed that 2AE can capture the patterns of the minority class without incorporating an extra process for class balancing. 2AE yields adequate performance and outperforms other more mainstream approaches, resulting in an AUPRC score of 0.481. Conclusions We have demonstrated how machine learning can be employed to analyze both structured and unstructured data from electronic health records for the purpose of preventable ADE prediction. The developed algorithm 2AE can be used to effectively learn minority group phenotype from imbalanced data

Anti-infectious and anti-inflammatory effects of peptide fragments sequentially derived from the antimicrobial peptide centrocin 1 isolated from the green sea urchin, Strongylocentrotus droebachiensis

Bacterial resistance against antibiotic treatment has become a major threat to public health. Antimicrobial peptides (AMPs) have emerged as promising alternative agents for treatment of infectious diseases. This study characterizes novel synthetic peptides sequentially derived from the AMP centrocin 1, isolated from the green sea urchin, for their applicability as anti-infective agents.The microbicidal effect of centrocin 1 heavy chain (CEN1 HC-Br), its debrominated analogue (CEN1 HC), the C-terminal truncated variants of both peptides, i.e. CEN1 HC-Br (1--20) and CEN1 HC (1--20), as well as the cysteine to serine substituted equivalent CEN1 HC (Ser) was evaluated using minimal microbicidal concentration assay. The anti-inflammatory properties were assessed by measuring the inhibition of secretion of pro-inflammatory cytokines. All the peptides tested exhibited marked microbicidal and anti-inflammatory properties. No difference in efficacy was seen comparing CEN1 HC-Br and CEN1 HC, while the brominated variant had higher cytotoxicity. C-terminal truncation of both peptides reduced salt-tolerability of the microbicidal effect as well as anti-inflammatory actions. Also, serine substitution of cysteine residue decreased the microbicidal effect. Thus, from the peptide variants tested, CEN1 HC showed the best efficacy and safety profile. Further, CEN1 HC significantly reduced bacterial counts in two different animal models of infected wounds, while Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) failed to develop resistance against this peptide under continued selection pressure. In summary, CEN1 HC appears a promising new antimicrobial agent, and clinical studies are warranted to evaluate the applicability of this AMP for local treatment of infections in man

Structure-activity relationships of the antimicrobial peptide arasin 1 - and mode of action studies of the N terminal, proline-rich region

Arasin 1 is a 37 amino acid long proline-rich antimicrobial peptide isolated from the spider crab, Hyas araneus. In this work the active region of arasin 1 was identified through structure-activity studies using different peptide fragments derived from the arasin 1 sequence. The pharmacophore was found to be located in the proline/arginine-rich NH2 terminus of the peptide and the fragment arasin 1(1–23) was almost equally active to the full length peptide. Arasin 1 and its active fragment arasin 1(1–23) were shown to be non-toxic to human red blood cells and arasin 1(1–23) was able to bind chitin, a component of fungal cell walls and the crustacean shell. The mode of action of the fully active N-terminal arasin 1(1–23) was explored through killing kinetic and membrane permeabilization studies. At the minimal inhibitory concentration (MIC), arasin 1(1–23) was not bactericidal and had no membrane disruptive effect. In contrast, at concentrations of 5×MIC and above it was bactericidal and interfered with membrane integrity. We conclude that arasin 1(1–23) has a different mode of action than lytic peptides, like cecropin P1. Thus, we suggest a dual mode of action for arasin 1(1–23) involving membrane disruption at peptide concentrations above MIC, and an alternative mechanism of action, possibly involving intracellular targets, at MIC

The Investigation of Structure Heterogeneous Joint Welds in Pipelines

Welding joints of dissimilar steels don’t withstand design life. One of the important causes of premature destructions can be the acceleration of steel structural degradation due to cyclic mechanical and thermal gradients. Two zones of tube from steel 12H18N9T, exhibiting the structural instability at early stages of the decomposition of a supersaturated solid austenite solution, were subjected to investigation. Methods of x-ray spectral and structure analysis, micro hardnessmetry were applied for the research. Made the following conclusions, inside and outside tube wall surfaces of hazardous zones in welding joint have different technological and resource characteristics. The microhardness very sensitive to changes of metal structure and can be regarded as integral characteristic of strength and ductility. The welding processes are responsible for the further fibering of tube wall structure, they impact to the characteristics of hot-resistance and long-term strength due to development of ring cracks in the welding joint of pipeline. The monitoring of microhardness and structural phase conversions can be used for control by changes of mechanical properties in result of post welding and reductive heat treatment of welding joints

Investigation of tetrasubstituted heterocycles reveals hydantoins as a promising scaffold for development of novel antimicrobials with membranolytic properties

Mimics of antimicrobial peptides (AMPs) have been proposed as a promising class of antimicrobial agents. We report the analysis of five tetrasubstituted, cationic, amphipathic heterocycles as potential AMP mimics. The analysis showed that the heterocyclic scaffold had a strong influence on the haemolytic activity of the compounds, and the hydantoin scaffold was identified as a promising template for drug lead development. Subsequently, a total of 20 hydantoin derivatives were studied for their antimicrobial potency and haemolytic activity. We found 19 of these derivatives to have very low haemolytic toxicity and identified three lead structures, 2dA, 6cG, and 6dG with very promising broad-spectrum antimicrobial activity. Lead structure 6dG displayed minimum inhibitory concentration (MIC) values as low as 1 μg/mL against Gram-positive bacteria and 4–16 μg/mL against Gram-negative bacteria. Initial mode of action (MoA) studies performed on the amine derivative 6cG, utilizing a luciferase-based biosensor assay, suggested a strong membrane disrupting effect on the outer and inner membrane of Escherichia coli. Our findings show that the physical properties and structural arrangement induced by the heterocyclic scaffolds are important factors in the design of AMP mimics

A concise SAR-analysis of antimicrobial cationic amphipathic barbiturates for an improved activity-toxicity profile

An amphipathic barbiturate mimic of the marine eusynstyelamides is reported as a promising class of antimicrobial agents. We hereby report a detailed analysis of the structure-activity relationship for cationic amphipathic N,N′ -dialkylated-5,5-disubstituted barbiturates. The influence of various cationic groups, hydrocarbon linkers and lipophilic side chains on the compounds’ antimicrobial potency and haemolytic activity was studied. A comprehensive library of 58 compounds was prepared using a concise synthetic strategy. We found cationic amine and guanidyl groups to yield the highest broad-spectrum activity and cationic trimethylated quaternary amine groups to exert narrow-spectrum activity against Gram-positive bacteria. n-Propyl hydrocarbon linkers proved to be the best compromise between potency and haemolytic activity. The combination of two different lipophilic side chains allowed for further fine-tuning of the biological properties. Using these insights, we were able to prepare both, the potent narrow-spectrum barbiturate 8a and the broad-spectrum barbiturates 11lG, 13jA and 13jG, all having low or no haemolytic activity. The guanidine derivative 11lG demonstrated a strong membrane disrupting effect in luciferase-based assays. We believe that these results may be valuable in further development of antimicrobial lead structures