614 research outputs found

    Faculty Artist Recital: Max Schoenfeld, Flute; Russell Bedford, Bassoon; July 23, 1973

    Get PDF
    Centennial East Recital HallMonday EveningJuly 23, 19738:15 p.m

    PCR for the detection of pathogens in neonatal early onset sepsis.

    Get PDF
    BACKGROUND: A large proportion of neonates are treated for presumed bacterial sepsis with broad spectrum antibiotics even though their blood cultures subsequently show no growth. This study aimed to investigate PCR-based methods to identify pathogens not detected by conventional culture. METHODS: Whole blood samples of 208 neonates with suspected early onset sepsis were tested using a panel of multiplexed bacterial PCRs targeting Streptococcus pneumoniae, Streptococcus agalactiae (GBS), Staphylococcus aureus, Streptococcus pyogenes (GAS), Enterobacteriaceae, Enterococcus faecalis, Enterococcus faecium, Ureaplasma parvum, Ureaplasma urealyticum, Mycoplasma hominis and Mycoplasma genitalium, a 16S rRNA gene broad-range PCR and a multiplexed PCR for Candida spp. RESULTS: Two-hundred and eight samples were processed. In five of those samples, organisms were detected by conventional culture; all of those were also identified by PCR. PCR detected bacteria in 91 (45%) of the 203 samples that did not show bacterial growth in culture. S. aureus, Enterobacteriaceae and S. pneumoniae were the most frequently detected pathogens. A higher bacterial load detected by PCR was correlated positively with the number of clinical signs at presentation. CONCLUSION: Real-time PCR has the potential to be a valuable additional tool for the diagnosis of neonatal sepsis

    Methodological considerations of integrating portable digital technologies in the analysis and management of complex superimposed Californian pictographs: From spectroscopy and spectral imaging to 3-D scanning

    Get PDF
    How can the utilization of newly developed advanced portable technologies give us greater understandings of the most complex of prehistoric rock art? This is the questions driving The Gordian Knot project analysing the polychrome Californian site known as Pleito. New small transportable devices allow detailed on-site analyses of rock art. These non-destructive portable technologies can use X-ray and Raman technology to determine the chemical elements used to make the pigment that makes the painting; they can use imaging techniques such as Highlight Reflective Transformation Imaging and dStretch© to enhance their visibility; they can use digital imagery to disentangle complex superimposed paintings; and they can use portable laser instruments to analyse the micro-topography of the rock surface and integrate these technologies into a 3-D environment. This paper outlines a robust methodology and preliminary results to show how an integration of different portable technologies can serve rock art research and management
    corecore