Decreasing initial telomere length in humans intergenerationally understates age-associated telomere shortening

Telomere length shortens with aging, and short telomeres have been linked to a wide variety of pathologies. Previous studies suggested a discrepancy in age-associated telomere shortening rate estimated by cross-sectional studies versus the rate measured in longitudinal studies, indicating a potential bias in cross-sectional estimates. Intergenerational changes in initial telomere length, such as that predicted by the previously described effect of a father's age at birth of his offspring (FAB), could explain the discrepancy in shortening rate measurements. We evaluated whether changes occur in initial telomere length over multiple generations in three large datasets and identified paternal birth year (PBY) as a variable that reconciles the difference between longitudinal and cross-sectional measurements. We also clarify the association between FAB and offspring telomere length, demonstrating that this effect is substantially larger than reported in the past. These results indicate the presence of a downward secular trend in telomere length at birth over generational time with potential public health implications

Looking at sound: sound design and the audiovisual influences on gaze

Book synopsis: Seeing into Screens: Eye Tracking and the Moving Image is the first dedicated anthology that explores vision and perception as it materializes as viewers watch screen content. While nearly all moving image research either 'imagines' how its audience responds to the screen, or focuses upon external responses, this collection utilizes the data produced from eye tracking technology to assess seeing and knowing, gazing and perceiving. The editors divide their collection into the following four sections: eye tracking performance, which addresses the ways viewers respond to screen genre, actor and star, auteur, and cinematography; eye tracking aesthetics which explores the way viewers gaze upon colour, light, movement, and space; eye tracking inscription, which examines the way the viewer responds to subtitles, translation, and written information found in the screen world; and eye tracking augmentation which examines the role of simulation, mediation, and technological intervention in the way viewers engage with screen content. At a time when the nature of viewing the screen is extending and diversifying across different platforms and exhibitions, Seeing into Screens is a timely exploration of how viewers watch the screen

Looking at Sound: Sound Design and the Audiovisual Influences on Gaze

From the earliest films to the blockbusters of today, film has rarely been silent. Live musical accompaniment of silent movies progressed into the synchronized sound of ‘talkies’ and today film sound is a highly developed craft in which sound-designers believe they have the power to represent and accentuate aspects of the scene, focusing the viewer’s attention to specific events and conveying emotion (e.g. Bordwell & Thompson, 2013; Chion, 1994; Murch, 2001). This chapter will attempt to empirically validate some of these beliefs by exploring the separate and integrated influence of each of the primary auditory components of a film’s sound design (musical score, dialogue and sound effects; known as “sound stems”) on viewer behavior, specifically observing the role of sound in guiding a viewer’s gaze through a film. This chapter will approach these issues from the perspective of experimental cognitive psychology. For a review of sound design practice see Sonnenschein, (2001); for a review of the psychological impacts of music and sound see Cohen (2014) and for reviews of film theory on classic and modern sound design see Gorbman (1980) and Donnelly (2009), respectively. This chapter considers the influence of sound design in two “found” experimental case studies in which filmmakers claim to have manipulated viewer behavior through sound design. Firstly, a highly dynamic and edited scene from How to Train Your Dragon (DeBlois & Sanders 2010) was viewed with the three sound stems independently (dialogue, sound effects, music and a silent condition), the attentional synchrony and affective responses between the sound conditions will be compared. Secondly, gaze behavior during the famous single long opening shot from The Conversation (Coppola 1974) compared the sound influences (the presence and absence of sound) during discrete sound events within the sequence and viewer gaze behavior via quantitative analysis of heat-maps. We conclude that the influence of sound on viewer gaze during film viewing is not as pronounced as often thought. Future studies are required to further our understanding of the nuanced influence of sound design and how it shapes our whole experience of a film including attention and affective responses

Saccades predict and synchronize to visual rhythms irrespective of musical beats

Music has been shown to entrain movement. One of the body’s most frequent movements, saccades, are arguably subject to a timer that may also be susceptible to musical entrainment. We developed a continuous and highly-controlled visual search task and varied the timing of the search target presentation, it was either gaze-contingent, tap-contingent, or visually-timed. We found: (1) explicit control of saccadic timing is limited to gross duration variations and imprecisely synchronized; (2) saccadic timing does not implicitly entrain to musical beats, even when closely aligned in phase; (3) eye movements predict visual onsets produced by motor-movements (finger-taps) and externally-timed sequences, beginning fixation prior to visual onset; (4) eye movement timing can be rhythmic, synchronizing to both motor-produced and externally timed visual sequences; each unaffected by musical beats. These results provide evidence that saccadic timing is sensitive to the temporal demands of visual tasks and impervious to influence from musical beats

Surface enhanced Raman scattering of crystal violet

Despite the ubiquity of Raman spectroscopy, fluorescence, poor signal strength and photobleaching pose a significant challenge to researchers in the biomedical field. Here, we demonstrate a 17-fold signal enhancement in Raman spectra of crystal violet via surface-enhanced Raman scattering (SERS). The SERS substrate was fabricated by electron beam lithography (EBL); the nanostructured surface was an array of G-shaped elements made of Au on SiO2/Si. In addition to the SERS spectra, finite-difference time-domain simulations were performed to illustrate the distribution of electric-field hot-spots on the SERS substrate. The electric-field hot-spots were prominent at the vertices and edges of the nanostructured G-shaped motifs. The results presented here demonstrate that EBL is a high-end choice for SERS substrate fabrication that opens the way for more complex Raman spectroscopies, for instance involving nonlinear optics or chiral analytes

Thick, Adherent Diamond Films on AlN with Low Thermal Barrier Resistance.

The growth of >100-μm-thick diamond layers adherent on aluminum nitride with low thermal boundary resistance between diamond and AlN is presented in this work. The thermal barrier resistance was found to be in the range of 16 m2·K/GW, which is a large improvement on the current state-of-the-art. While thick films failed to adhere on untreated AlN films, AlN films treated with hydrogen/nitrogen plasma retained the thick diamond layers. Clear differences in ζ-potential measurement confirm surface modification due to hydrogen/nitrogen plasma treatment. An increase in non-diamond carbon in the initial layers of diamond grown on pretreated AlN is seen by Raman spectroscopy. The presence of non-diamond carbon has minimal effect on the thermal barrier resistance. The surfaces studied with X-ray photoelectron spectroscopy revealed a clear distinction between pretreated and untreated samples. The surface aluminum goes from a nitrogen-rich environment to an oxygen-rich environment after pretreatment. A clean interface between diamond and AlN is seen by cross-sectional transmission electron microscopy

Excitonic and lattice contributions to the charge density wave in 1T-TiSe2 revealed by a phonon bottleneck

Understanding collective electronic states such as superconductivity and charge density waves is pivotal for fundamental science and applications. The layered transition metal dichalcogenide 1T-TiSe2 hosts a unique charge density wave (CDW) phase transition whose origins are still not fully understood. Here, we present ultrafast time- and angle-resolved photoemission spectroscopy (TR-ARPES) measurements complemented by time-resolved reflectivity (TRR) which allows us to establish the contribution of excitonic and electron-phonon interactions to the CDW. We monitor the energy shift of the valence band (VB) and coupling to coherent phonons as a function of laser fluence. The VB shift, directly related to the CDW gap closure, exhibits a markedly slower recovery dynamics at fluences above Fth = 60 microJ cm-2. This observation coincides with a shift in the relative weight of coherently coupled phonons to higher frequency modes in time-resolved reflectivity (TRR), suggesting a phonon bottleneck. Using a rate equation model, the emergence of a high-fluence bottleneck is attributed to an abrupt reduction in coupled phonon damping and an increase in exciton dissociation rate linked to the loss of CDW superlattice phonons. Thus, our work establishes the important role of both excitonic and phononic interactions in the CDW phase transition and the advantage of combining complementary femtosecond techniques to understand the complex interactions in quantum materials.Comment: 11 pages, 4 figure

Sequencing and Analysis of Lumpy Skin Disease Virus Whole Genomes Reveals a New Viral Subgroup in West and Central Africa

Lumpy skin disease virus (LSDV) is a member of the capripoxvirus (CPPV) genus of the Poxviridae family. LSDV is a rapidly emerging, high-consequence pathogen of cattle, recently spreading from Africa and the Middle East into Europe and Asia. We have sequenced the whole genome of historical LSDV isolates from the Pirbright Institute virus archive, and field isolates from recent disease outbreaks in Sri Lanka, Mongolia, Nigeria and Ethiopia. These genome sequences were compared to published genomes and classified into different subgroups. Two subgroups contained vaccine or vaccine-like samples ("Neethling-like" clade 1.1 and "Kenya-like" subgroup, clade 1.2.2). One subgroup was associated with outbreaks of LSD in the Middle East/Europe (clade 1.2.1) and a previously unreported subgroup originated from cases of LSD in west and central Africa (clade 1.2.3). Isolates were also identified that contained a mix of genes from both wildtype and vaccine samples (vaccine-like recombinants, grouped in clade 2). Whole genome sequencing and analysis of LSDV strains isolated from different regions of Africa, Europe and Asia have provided new knowledge of the drivers of LSDV emergence, and will inform future disease control strategies.</p