<i>Sinorhizobium fredii</i> cultured in media supplemented with <i>Amaranthus cruentus</i> L. seed meal and bacterial cell survival in liquid and peat based inoculum

Nine different growth media were evaluated to determine the best growth conditions to achieve cultures of a high cell number of fast-growing rhizobia to produce inoculants. We found that Sinorhizobium fredii strains have complex nutritional requirements that were fulfilled by adding to the media 4 g Amaranthus cruentus L. seed meal/l. The survival of fast growing strains is a variable trait, but those strains that survived at high levels even after 6-month storage, hypernodulated soybeans and fixed atmospheric nitrogen at levels as high as those of Bradyrhizobium japonicum.Instituto de Fisiología Vegeta

Interoperabilidad HL7 con un sistema de laboratorio externo

Se presenta la implementación de una plataforma de interoperabilidad entre el sistema de información de SUAT (SIGS) y el Laboratorio de Análisis Clínicos Biofast para la gestión de la solicitud de exámenes desde la indicación en la Historia Cínica Electrónica (HCE), el agendamiento del usuario, la recolección de las muestras, el envío de la solicitud y la posterior recepción de los resultados e integración a la HCE.Sociedad Argentina de Informática e Investigación Operativ

Interoperabilidad HL7 con un sistema de laboratorio externo

Se presenta la implementación de una plataforma de interoperabilidad entre el sistema de información de SUAT (SIGS) y el Laboratorio de Análisis Clínicos Biofast para la gestión de la solicitud de exámenes desde la indicación en la Historia Cínica Electrónica (HCE), el agendamiento del usuario, la recolección de las muestras, el envío de la solicitud y la posterior recepción de los resultados e integración a la HCE.Sociedad Argentina de Informática e Investigación Operativ

Characterization of the overlapping promoters of nolB and nolW, two soybean cultivar specificity genes from Rhizobium fredii strain USDA257

The transcripts of nolW and nolB, two divergently oriented cultivar specificity genes of Rhizobium fredii strain USDA257, are known to be initiated 14 bp apart from promoters that face one another. We show here that expression of nolB is dependent both on induction with flavonoid signals and on the regulatory gene, nodD1. Expression of nolW is constitutive and independent of flavonoids and nodD1. Normal expression of nolB is retained with a promoter that extends only 61 bp upstream of the transcript start site, but it is lost if an additional 24 bp are removed. Substantial expression of nolW is retained with a promoter that contains only 34 bp of DNA upstream from the transcript initiation site. The dual control region for the two genes is thus only about 109 bp in length.Instituto de Fisiología Vegeta

Cultivos de Bradyrhizobium japonicum y Sinorhizobium melitoti en medios con hojas de Amaranthus cruentus, como aporte de factores de crecimiento

p.43-50En este trabajo se estudió la obtención de suspensiones de Sinorhizobium meliloti y Bradyrhizobium japonicum, considerando el efecto de la harina de hoja de amaranto sobre el crecimiento celular en medios recomendados por distintos autores. Los experimentos se realizaron en erlenmeyers en agitador rotatorio a 250 rpm y 2,5 cm de excentricidad. Los estudios realizados permitieron establecer medios de cultivo para alcanzar concentraciones celulares del orden de 2 x 10¹° células viables/ml. Los resultados obtenidos muestran que es posible reemplazar los 4 g/l de extracto de levadura en los medios típicos por 4g/l de harina de hoja de Amaranthus cruentus o sus extractos acuosos. Con las suspensiones obtenidas de cada microorganismo se prepararon inoculantes utilizando como soportes, turba y perlita. La sobrevivencia de las cepas sobre los soportes indicados, a los seis meses, mostraron una concentración de 9x10(9) a 2x10¹° células viables/g, manteniendo los microorganismos sus propiedades simbióticas

A survey on occurrence of <i>Cladosporium fulvum</i> identifies race 0 and race 2 in tomato growing areas of Argentina

The presence of Cladosporium fulvum (syn. Passalora fulva), causal agent of tomato leaf mold, was confirmed in the two main greenhouseproduction areas for tomato in Argentina. Using both morphological characters and internal transcribed spacer sequencing, we confirmed the presence of physiological races of this pathogen. A diagnostic multiplex polymerase chain reaction (PCR) was also developed, using primers derived from C. fulvum avirulence (Avr) genes. In all, 20 isolates of Cladosporium spp. were obtained as monospore cultures and 12 were identified as C. fulvum. By this method, we showed that, of these 12 isolates, 5 were race 0 (carrying functional Avr2, Avr4, Avr4E, and Avr9 genes) and 7 were race 2 (lacking the Avr2 gene). Race identity was confirmed by testing their virulence on a set of tomato differentials carrying different Cf resistance genes. All Avr genes could be amplified in single or multiplex PCR using DNA isolated from in vitro grown monospore cultures but only three Avr could be amplified when genomic DNA was isolated from C. fulvum-infected necrotic leaf tissue.Facultad de Ciencias Agrarias y Forestale

Deregulation of miRNAs in malignant pleural mesothelioma is associated with prognosis and suggests an alteration of cell metabolism

Malignant pleural mesothelioma (MPM) is an aggressive human cancer and miRNAs can play a key-role for this disease. In order to broaden the knowledge in this field, the miRNA expression was investigated in a large series of MPM to discover new pathways helpful in diagnosis, prognosis and therapy. We employed nanoString nCounter system for miRNA profiling on 105 MPM samples and 10 healthy pleura. The analysis was followed by the validation of the most significantly deregulated miRNAs by RT-qPCR in an independent sample set. We identified 63 miRNAs deregulated in a statistically significant way. MiR-185, miR-197, and miR-299 were confirmed differentially expressed, after validation study. In addition, the results of the microarray analysis corroborated previous findings concerning miR-15b-5p, miR-126-3p, and miR-145-5p. Kaplan-Meier curves were used to explore the association between miRNA expression and overall survival (OS) and identified a 2-miRNA prognostic signature (Let-7c-5p and miR-151a-5p) related to hypoxia and energy metabolism respectively. In silico analyses with DIANA-microT-CDS highlighted 5 putative targets in common between two miRNAs. With the present work we showed that the pattern of miRNAs expression is highly deregulated in MPM and that a 2-miRNA signature can be a new useful tool for prognosis in MPM

Identification of miRNA-103 in the Cellular Fraction of Human Peripheral Blood as a Potential Biomarker for Malignant Mesothelioma – A Pilot Study

Background: To date, no biomarkers with reasonable sensitivity and specificity for the early detection of malignant mesothelioma have been described. The use of microRNAs (miRNAs) as minimally-invasive biomarkers has opened new opportunities for the diagnosis of cancer, primarily because they exhibit tumor-specific expression profiles and have been commonly observed in blood of both cancer patients and healthy controls. The aim of this pilot study was to identify miRNAs in the cellular fraction of human peripheral blood as potential novel biomarkers for the detection of malignant mesothelioma. Methodology/Principal Findings: Using oligonucleotide microarrays for biomarker identification the miRNA levels in the cellular fraction of human peripheral blood of mesothelioma patients and asbestos-exposed controls were analyzed. Using a threefold expression change in combination with a significance level of p,0.05, miR-103 was identified as a potential biomarker for malignant mesothelioma. Quantitative real-time PCR (qRT-PCR) was used for validation of miR-103 in 23 malignant mesothelioma patients, 17 asbestos-exposed controls, and 25 controls from the general population. For discrimination of mesothelioma patients from asbestos-exposed controls a sensitivity of 83 % and a specificity of 71 % were calculated, and for discrimination of mesothelioma patients from the general population a sensitivity of 78 % and a specificity of 76%

La renovación de la palabra en el bicentenario de la Argentina : los colores de la mirada lingüística

El libro reúne trabajos en los que se exponen resultados de investigaciones presentadas por investigadores de Argentina, Chile, Brasil, España, Italia y Alemania en el XII Congreso de la Sociedad Argentina de Lingüística (SAL), Bicentenario: la renovación de la palabra, realizado en Mendoza, Argentina, entre el 6 y el 9 de abril de 2010. Las temáticas abordadas en los 167 capítulos muestran las grandes líneas de investigación que se desarrollan fundamentalmente en nuestro país, pero también en los otros países mencionados arriba, y señalan además las áreas que recién se inician, con poca tradición en nuestro país y que deberían fomentarse. Los trabajos aquí publicados se enmarcan dentro de las siguientes disciplinas y/o campos de investigación: Fonología, Sintaxis, Semántica y Pragmática, Lingüística Cognitiva, Análisis del Discurso, Psicolingüística, Adquisición de la Lengua, Sociolingüística y Dialectología, Didáctica de la lengua, Lingüística Aplicada, Lingüística Computacional, Historia de la Lengua y la Lingüística, Lenguas Aborígenes, Filosofía del Lenguaje, Lexicología y Terminología