2,838 research outputs found
Arabidopsis SYT1 maintains stability of cortical endoplasmic reticulum networks and VAP27-1-enriched endoplasmic reticulumâplasma membrane contact sites
Arabidopsis synaptotagmin 1 (SYT1) is localized on the endoplasmic reticulumâplasma membrane (ERâPM) contact sites in leaf and root cells. The ERâPM localization of Arabidopsis SYT1 resembles that of the extended synaptotagmins (E-SYTs) in animal cells. In mammals, E-SYTs have been shown to regulate calcium signaling, lipid transfer, and endocytosis. Arabidopsis SYT1 was reported to be essential for maintaining cell integrity and virus movement. This study provides detailed insight into the subcellular localization of SYT1 and VAP27-1, another ERâPM-tethering protein. SYT1 and VAP27-1 were shown to be localized on distinct ERâPM contact sites. The VAP27-1-enriched ERâPM contact sites (V-EPCSs) were always in contact with the SYT1-enriched ERâPM contact sites (S-EPCSs). The V-EPCSs still existed in the leaf epidermal cells of the SYT1 null mutant; however, they were less stable than those in the wild type. The polygonal networks of cortical ER disassembled and the mobility of VAP27-1 protein on the ERâPM contact sites increased in leaf cells of the SYT1 null mutant. These results suggest that SYT1 is responsible for stabilizing the ER network and V-EPCSs
The J_1-J_2 antiferromagnet with Dzyaloshinskii-Moriya interaction on the square lattice: An exact diagonalization study
We examine the influence of an anisotropic interaction term of
Dzyaloshinskii-Moriya (DM) type on the groundstate ordering of the J_1-J_2
spin-1/2-Heisenberg antiferromagnet on the square lattice. For the DM term we
consider several symmetries corresponding to different crystal structures. For
the pure J_1-J_2 model there are strong indications for a quantum spin liquid
in the region of 0.4 < J_2/J_1 < 0.65. We find that a DM interaction influences
the breakdown of the conventional antiferromagnetic order by i) shifting the
spin liquid region, ii) changing the isotropic character of the groundstate
towards anisotropic correlations and iii) creating for certain symmetries a net
ferromagnetic moment.Comment: 7 pages, RevTeX, 6 ps-figures, to appear in J. Phys.: Cond. Ma
Integral representation of the linear Boltzmann operator for granular gas dynamics with applications
We investigate the properties of the collision operator associated to the
linear Boltzmann equation for dissipative hard-spheres arising in granular gas
dynamics. We establish that, as in the case of non-dissipative interactions,
the gain collision operator is an integral operator whose kernel is made
explicit. One deduces from this result a complete picture of the spectrum of
the collision operator in an Hilbert space setting, generalizing results from
T. Carleman to granular gases. In the same way, we obtain from this integral
representation of the gain operator that the semigroup in L^1(\R \times \R,\d
\x \otimes \d\v) associated to the linear Boltzmann equation for dissipative
hard spheres is honest generalizing known results from the first author.Comment: 19 pages, to appear in Journal of Statistical Physic
Actin3 promoter reveals undulating F-actin bundles at shanks and dynamic F-actin meshworks at tips of tip-growing pollen tubes
The dynamic actin cytoskeleton of pollen tubes is both the driver of the tip growth and the organizer of cell polarity. In order to understand this fast re-arranging cytoskeletal system, we need reliable constructs expressed under relevant promoters. Here we are reporting that the Lifeact reporter, expressed under the pollen-specific Actin3 promoter, visualizes very dynamic F-actin elements both in germinating pollen grains and tip-growing pollen tubes. Importantly, we have documented very active actin polymerization at the cell periphery, especially in the bulging area during pollen germination and in the apical clear zone. Expression of the Lifeact reporter under control of the pollen-specific Actin3 promoter revealed 2 new aspects: (i) long F-actin bundles in pollen tube shanks are dynamic, showing undulating movements, (ii) subapical âactin collarsâ or âfringesâ are absent
Coordination Dependence of Hyperfine Fields of 5sp Impurities on Ni Surfaces
We present first-principles calculations of the magnetic hyperfine fields H
of 5sp impurities on the (001), (111), and (110) surfaces of Ni. We examine the
dependence of H on the coordination number by placing the impurity in the
surfaces, on top of them at the adatom positions, and in the bulk. We find a
strong coordination dependence of H, different and characteristic for each
impurity. The behavior is explained in terms of the on-site s-p hybridization
as the symmetry is reduced at the surface. Our results are in agreement with
recent experimental findings.Comment: 4 pages, 3 figure
Magnetic Proximity Effect in Perovskite Superconductor/Ferromagnet Multilayers
superconducting/ferromagnetic
(SC/FM) multilayers have been studied by neutron reflectometry. Evidence for a
characteristic difference between the structural and magnetic depth profiles is
obtained from the occurrence of a structurally forbidden Bragg peak in the FM
state. The comparison with simulated reflectivity curves allows us to identify
two possible magnetization profiles: a sizable magnetic moment within the SC
layer antiparallel to the one in the FM layer (inverse proximity effect), or a
``dead'' region in the FM layer with zero net magnetic moment. The former
scenario is supported by an anomalous SC-induced enhancement of the
off-specular reflection, which testifies to a strong mutual interaction of SC
and FM order parameters.Comment: 4 pages, 2 figures, submitted to PR
Cosmic shear requirements on the wavelength dependence of telescope point spread functions
Cosmic shear requires high precision measurement of galaxy shapes in the presence of the observational point spread function (PSF) that smears out the image. The PSF must therefore be known for each galaxy to a high accuracy. However, for several reasons, the PSF is usually wavelength dependent; therefore, the differences between the spectral energy distribution of the observed objects introduce further complexity. In this paper, we investigate the effect of the wavelength dependence of the PSF, focusing on instruments in which the PSF size is dominated by the diffraction limit of the telescope and which use broad-band filters for shape measurement. We first calculate biases on cosmological parameter estimation from cosmic shear when the stellar PSF is used uncorrected. Using realistic galaxy and star spectral energy distributions and populations and a simple three-component circular PSF, we find that the colour dependence must be taken into account for the next generation of telescopes. We then consider two different methods for removing the effect: (i) the use of stars of the same colour as the galaxies and (ii) estimation of the galaxy spectral energy distribution using multiple colours and using a telescope model for the PSF. We find that both of these methods correct the effect to levels below the tolerances required for per cent level measurements of dark energy parameters. Comparison of the two methods favours the template-fitting method because its efficiency is less dependent on galaxy redshift than the broad-band colour method and takes full advantage of deeper photometr
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