DSK1, a novel kinesin-related protein from the diatom Cylindrotheca fusiformis that is involved in anaphase spindle elongation.

We have identified an 80-kD protein that is involved in mitotic spindle elongation in the diatom Cylindrotheca fusiformis. DSK1 (Diatom Spindle Kinesin 1) was isolated using a peptide antibody raised against a conserved region in the motor domain of the kinesin superfamily. By sequence homology, DSK1 belongs to the central motor family of kinesin-related proteins. Immunoblots using an antibody raised against a non-conserved region of DSK1 show that DSK1 is greatly enriched in mitotic spindle preparations. Anti-DSK1 stains in diatom central spindle with a bias toward the midzone, and staining is retained in the spindle midzone during spindle elongation in vitro. Furthermore, preincubation with anti-DSK1 blocks function in an in vitro spindle elongation assay. This inhibition of spindle elongation can be rescued by preincubating concurrently with the fusion protein against which anti-DSK1 was raised. We conclude that DSK1 is involved in spindle elongation and is likely to be responsible for pushing hal-spindles apart in the spindle midzone

Trade Studies Relating to a Long Range Mach 2.6 Supercruiser

A systems study was conducted on an aircraft concept, representative of a supersonic-cruise military aircraft (supercruiser). The study results indicate that supersonic ranges in excess of 4000 n.mi. at a Mach number of 2.62 are possible with a 500 lbf class aircraft. Trade studies, to determine the sensitivity of supersonic range to parameters which would improve maneuverability, indicate that thrust-weight ratios of as much as 0.5 can be used without significantly decreasing supersonic range; however, increasing the thrust-weight ratio to 1.0 decreases the range capability by about 1100 n.mi. The range penalty for increasing the aircraft limit load-factor from 4.0 to 9.0 is about 500 n.mi. The increased fuel volume of several configurations improved the supersonic range capability by about 1200 n.mi. but, due to associated losses in supersonic L/D, had an insignificant effect on the range at a Mach number of 2.62

The episcopate of Richard De Kellawe, Bishop of Durham 1311-16

In 1311, Richard de Kellawe became Bishop of Durham, the last Benedictine monk to ascend the Episcopal throne. He emerged from a decade or more of bitter strife between the convent and Bishop Bek, the choice of the monks, to an episcopate under constant strain from the depredations of the Scots. This was the time of Robert Bruce and Bannockburn. Kellawe against Bruce seemed weak and helpless; hence his episcopate has been viewed as that of a pious and incompetent ecclesiastic, unfitted for the ways of the world. The present study, firmly based on the Bishop’s Register, attempts to show how this is untrue; how in fact Kellawe coped as well as could be expected with the Scottish depradations, and maintained a well-ordered diocese, was a capable administrator, and conducted relations with the King so that the rights and privileges of the regalian franchise of Durham were in 'no way compromised or impaired. It attempts to interpret Kellawe's position in terms of wider currents, especially in the light of recent work on the episcopate of his predecessors; his relationship to the convent in the background of his part in the struggle against Bek, his ability to become Bishop In relation to many factors, not least the character of Edward II and the distraction from a serious royal candidature for the bishopric caused by the Ordinances. It sees Kellawe as a worthy holder of the see, in spiritualia and temporalia, capable in his dealings, sincere in his wishes, realistic in his actions. It attempts to show too how his ultimate failure was not the result of his own deficiencies, but came about because the problems confronting him were too great for him to solve, and because his position was becoming increasingly incompatible with current tendencies

The influence of anaerobic muscle activity, maturation and season on the flesh quality of farmed turbot

In order to test seasonal, rearing, maturing and anaerobic muscle activity effect on the flesh quality of turbot (Scophthalmus maximus) a total of 80 farmed turbot from three different strains from reared under natural or continuous light were killed by a percussive blow to the head in November (winter, Icelandic strain), March (spring, Portuguese strain) and June (summer, domesticated strain (France turbot)). To test the effect of anaerobic muscle activity, 10 fish were on each occasion pre rigor filleted, where one fillet was used as a control, while the other fillet was electrically stimulated using a squared 5 Hz, 10 V pulsed DC for 3 min. All pre rigor fillets were measured for pH, weighed, wrapped in aluminum foil and stored in polystyrene boxes with ice. After 7 days of storage the fillets were measured instrumentally for pH, drip loss, colour (CIE L* a* b*) and texture properties such as hardness and shear force, while fillet shrinkage and colour (RBG) were evaluated with computer imaging on photographs from a standard lightbox. Results showed that softness of the flesh was mainly influenced by factors associated with growth, such as season, photoperiod and maturation. Anaerobic muscle activity simulated with electrical stimulation caused an increase in drip loss (<1%) and loss of shear force (<4%), but had no effect on hardness or fillet shrinkage. Computer imaging revealed that muscle contractions related to the electrical stimulus forced out blood from the fillet causing less reddishness for the entire storage period. We conclude that a pH drop upon slaughter associated with anaerobic muscle activity has a minor effect on the flesh quality in the short run, while seasonal/alternatively genetic effects are predominant

Precocious Puberty: Pathology, Related Risks, and Support Strategies

Children are currently starting pubertal development much earlier than 20 to 30 years ago. Data from the National Health and Nutrition Examination Survey (NHANES III) conducted from 1988-1994 revealed that the median age for breast development in white girls was approximately 9.7 years and 8 years in African-American girls versus 10.9 years ago. More recent data, however, indicate that some children are starting pubertal development even earlier (age 7 or 8 in girls or age 9 in boys). Very early pubertal development is identified as precocious puberty, a phenomenon that brings great concern to others besides parents: the child, their peers, school nurses, teachers and counselors. This paper discusses precocious pubertal development, possible causes or relationships, related risks, treatment and helping strategies