Chandra X-ray observation of the young stellar cluster NGC 3293 in the Carina Nebula Complex

We characterize the stellar population of the poorly explored young stellar cluster NGC 3293 at the northwestern periphery of the Carina Nebula Complex, in order to evaluate the cluster age and the mass function, and to test claims of an abnormal IMF and a deficit of M <= 2.5 M_sun stars. We performed a deep (70 ksec) X-ray observation of NGC 3293 with Chandra and detected 1026 individual X-ray point sources. We identify counterparts for 74% of the X-ray sources in deep near-infrared images. Our data clearly show that NGC 3293 hosts a large population of solar-mass stars, refuting claims of a lack of M <= 2.5 M_sun stars. The analysis of the color magnitude diagram suggests an age of ~8-10 Myr for the low-mass population of the cluster. There are at least 511 X-ray detected stars with color magnitude positions that are consistent with young stellar members within 7 arcmin of the cluster center. The number ratio of X-ray detected stars in the 1-2 M_sun range versus the M >= 5 M_sun stars (known from optical spectroscopy) is consistent with the expectation from a normal field initial mass function. Most of the early B-type stars and 20% of the later B-type stars are detected as X-ray sources. Our data shows that NGC 3293 is one of the most populous stellar clusters in the entire Carina Nebula Complex. The cluster probably harbored several O-type stars, whose supernova explosions may have had an important impact on the early evolution of the Carina Nebula Complex.Comment: accepted for Astronomy & Astrophysic

Structure of the Cytoplasmic Loop between Putative Helices II and III of the Mannitol Permease of Escherichia coli: A Tryptophan and 5-Fluorotryptophan Spectroscopy Study

In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EIImtl, are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy. The four positions, 97, 114, 126, and 133, are located in a region shown by recent studies to be involved in the mannitol translocation process. To spectroscopically distinguish between the Trp positions in each subunit of dimeric EIImtl, 5-FTrp was biosynthetically incorporated because of its much simpler photophysics compared to those of Trp. The steady-state and time-resolved fluorescence methodologies used point out that all four positions are in structured environments, both in the absence and in the presence of a saturating concentration of mannitol. The fluorescence decay of all 5-FTrp-containing mutants was highly homogeneous, suggesting similar microenvironments for both probes per dimer. However, Stern-Volmer quenching experiments using potassium iodide indicate different solvent accessibilities for the two probes at positions 97 and 133. A 5 Å two-dimensional (2D) projection map of the membrane-embedded IICmtl dimer showing 2-fold symmetry is available. The results of this work are in better agreement with a 7 Å projection map from a single 2D crystal on which no symmetry was imposed.

The first cytoplasmic loop of the mannitol permease from Escherichia coli is accessible for sulfhydryl reagents from the periplasmic side of the membrane

The mannitol permease (EIIMtl) from Escherichia coli couples mannitol transport to phosphorylation of the substrate. Renewed topology prediction of the membrane-embedded C domain suggested that EIIMtl contains more membrane-embedded segments than the six proposed previously on the basis of a PhoA fusion study. Cysteine accessibility was used to confirm this notion. Since cysteine 384 in the cytoplasmic B domain is crucial for the phosphorylation activity of EIIMtl, all cysteine mutants contained this activity-linked cysteine residue in addition to those introduced for probing the membrane topology of the protein. To distinguish between the activity-linked cysteine and the probed cysteine, either trypsin was used to specifically digest the two cytoplasmic domains (A and B), thereby removing Cys384, or Cys384 was protected by phosphorylation from alkylation by N-ethylmaleimide (NEM). Our data show that upon phosphorylation EIIMtl undergoes major conformational changes, whereby residues in the putative first cytoplasmic loop become accessible to NEM. Other residues in this loop were accessible to NEM in intact cells and inside-out membrane vesicles, but cysteine residues at these positions only reacted with the membrane-impermeable sulfhydryl reagent from the periplasmic side of the protein. These and other results suggest that the predicted loop between TM2 and TM3 may fold back into the membrane and form part of the translocation path

Luminous X-Ray Sources in Arp 147

The Chandra X-Ray Observatory was used to image the collisional ring galaxy Arp 147 for 42 ks. We detect 9 X-ray sources with luminosities in the range of 1.4 - 7 x 10^{39} ergs/sec in or near the blue knots of star formation associated with the ring. A source with an isotropic X-ray luminosity of 1.4 x 10^{40} ergs/sec is detected in the nuclear region of the intruder galaxy. X-ray sources associated with a foreground star and a background quasar are used to improve the registration of the X-ray image with respect to HST high resolution optical images. The intruder galaxy, which apparently contained little gas before the collision, shows no X-ray sources other than the one in the nuclear bulge which may be a poorly fed supermassive black hole. These observations confirm the conventional wisdom that collisions of gas rich galaxies trigger large rates of star formation which, in turn, generate substantial numbers of X-ray sources, some of which have luminosities above the Eddington limit for accreting stellar-massComment: 9 pages, 5 figure

X-ray view of IC348 in the light of an updated cluster census

We study the properties of the coronae of the low-mass stars in the young (~2-3Myr), nearby (~310pc) open cluster IC348 combining X-ray and optical/infrared data. The four existing Chandra observations of IC348 are merged, thus providing a deeper and spatially more complete X-ray view than previous X-ray studies of the cluster. We have compiled a comprehensive catalog of IC348 members taking into account recent updates to the cluster census. Our data collection comprises fundamental stellar parameters, infrared excess indicating the presence of disks, Halpha emission as a tracer of chromospheric emission or accretion and mass accretion rates. We have detected 290 X-ray sources in four merged Chandra exposures, of which 187 are associated with known cluster members. Only four of the X-ray sources are brown dwarfs (spectral type M6 and later). The detection rate is highest for diskless Class III stars and increases with stellar mass. This may be explained with higher X-ray luminosities for higher mass and later evolutionary stage that is evident in the X-ray luminosity functions. In particular, we find that for the lowest examined masses (0.1-0.25 Msun) there is a difference between the X-ray luminosity functions of accreting and non-accreting stars (classified on the basis of their Halpha emission strength) as well as those of disk-bearing and diskless stars (classified on the basis of the slope of the spectral energy distribution). These differences disappear for higher masses. This is related to our finding that the L_x/L_bol ratio is non-constant across the mass/luminosity sequence of IC348 with a decrease towards lower luminosity stars. Our analysis of an analogous stellar sample in the Orion Nebula Cluster suggests that the decline of L_x/L_ bol for young stars at the low-mass end of the stellar sequence is likely universal.Comment: Accepted for publication in Astronomy & Astrophysic

RNA expression profiling in brains of familial hemiplegic migraine type 1 knock-in mice

Background Various CACNA1A missense mutations cause familial hemiplegic migraine type 1 (FHM1), a rare monogenic subtype of migraine with aura. FHM1 mutation R192Q is associated with pure hemiplegic migraine, whereas the S218L mutation causes hemiplegic migraine, cerebellar ataxia, seizures, and mild head trauma-induced brain edema. Transgenic knock-in (KI) migraine mouse models were generated that carried either the FHM1 R192Q or the S218L mutation and were shown to exhibit increased CaV2.1 channel activity. Here we investigated their cerebellar and caudal cortical transcriptome. Methods Caudal cortical and cerebellar RNA expression profiles from mutant and wild-type mice were studied using microarrays. Respective brain regions were selected based on their relevance to migraine aura and ataxia. Relevant expression changes were further investigated at RNA and protein level by quantitative polymerase chain reaction (qPCR) and/or immunohistochemistry, respectively. Results Expression differences in the cerebellum were most pronounced in S218L mice. Particularly, tyrosine hydroxylase, a marker of delayed cerebellar maturation, appeared strongly upregulated in S218L cerebella. In contrast, only minimal expression differences were observed in the caudal cortex of either mutant mice strain. Conclusion Despite pronounced consequences of migraine gene mutations at the neurobiological level, changes in cortical RNA expression in FHM1 migraine mice compared to wild-type are modest. In contrast, pronounced RNA expression changes are seen in the cerebellum of S218L mice and may explain their cerebellar ataxia phenotyp