Conductas Delictivas Y Antisociales En Adolescentes Que Estudian Y No Estudian

Adolescence is a stage of transition from childhood to the adult world, which is affected among other things by the tension between the incorporation into the labor market and permanence in the educational system (D'alessandre, 2010). However, there is little information related to adolescents who do not study or work. Because of the information that antisocial and criminal behavior tends to accentuate at this stage of life (Gaeta & Galvanoski, 2011), this study identifies differences in the presence of antisocial and criminal behavior among students in two public high schools at Pachuca Hidalgo, Mexico, and adolescents who do not study or work in the same city using the AD measure (Seisdedos & Sánchez, 2001). The sample consisted of 120 teenagers between 14 and 18 years of age, with a total of 81 students and 39 adolescents who do not study or work. Through an analysis of variance ANOVA of sex by occupation were found significant differences in the presence of antisocial behaviors between adolescents who study and those who do not. A difference was observed in the presence of criminal behavior also, specifically in the group of male adolescents who did not study. It was observed that those adolescent men and women who are studying, are less likely to commit criminal acts, compared to those adolescent especially men who are not within a school or work system. The role played by the socio-cultural context as a risk factor or protection for the appearance of criminal and antisocial behavior is highlighted

Transcriptomic analysis of the interaction geminivirus-tomato

Geminiviridae family is one of the main families of plant pathogenic viruses with large relevance as they cause great losses worldwide in commercial crops and crops destined to food production. Geminiviruses present a little single-stranded DNA genome and a capsid composed of two twin icosahedral parts. Tomato Yellow Leaf Curl Virus (TYLCV) belongs to the Begomovirus genus and is transmitted by the whitefly Bemisia tabaci. With only 6 viral proteins, this geminivirus must create a proper environment for viral replication, transcription and propagation. Behind the apparent simplicity of geminiviruses lies a complex network of molecular interactions with their host and even their natural vector, which induces a wide variety of transcriptional, post-transcriptional and chromatinic changes in both the plant and the geminivirus. In order to study these changes and decipher the effects of the transmission vector on the infection, we carried out a global approximation of the TYLCV-tomato interaction to generate integrated single-base resolution maps by NGS (next-generation sequencing) of the transcriptome, smallRNAome and methylome of the pathogen and the host. Tomato plants (Moneymaker) were infected with TYLCV under controlled conditions of light and temperature using Agrobacterium tumefaciens or its natural vector. Apical tissue from these plants was collected at different time points (2, 7, 14 and 21 days after inoculation), and three biological replicas were generated for each treatment and time. Total RNA and DNA was extracted and analysed by RNA-Seq, smallRNA-Seq and Bisulfite-Seq. The transcriptome of the tomato-TYLCV interaction will be presented and discussed.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

Complex interaction among virus-plant-vector in the Tomato yellow leaf curl disease (TYLCD)

Geminiviruses constitute the largest plant family of DNA viruses that cause diseases in crops worldwide. Among them, Tomato yellow leaf curl disease (TYLCD) is one of the most devastating viral diseases affecting tomato crops in tropical, subtropical and temperate areas worldwide. Tomato yellow leaf curl virus (TYLCV), the first known causal agent of TYLCD is a monopartite Begomovirus transmitted by the whitefly Bemisia tabaci. In the recent years, several works have revealed a complex scenario in the interaction among the players that participate in the disease, the virus, the whitefly and the host plant, that may produce a different outcome depending on the interaction of each of the organism involved. To get insight to these three-way interaction, we have analysed the changes in the transcriptome, the smallRNA profile and the methylome of tomato plants infected with TYLCV either by agroinoculation of by B. tabaci. DNA and RNA samples were extracted from infected apical tomato leaves at 7, 14, 14 and 21 dpi (days post infection) and RNAseq, smallRNAseq and WGBS (Whole Genome Bisulfite Sequencing) was performed. Comparative analysis of the infection mediated by Agrobacterium and the whitefly lighted-up genes and DNA methylated regions, that were deregulated and in plants TYLCV-infected by B. tabaci and not by agroinculation, suggesting a partially different plants response to TYLCV depending on the infection method used.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Nanopartículas de quitosano: una prometedora estrategia para combatir la resistencia a antimicrobianos

Antimicrobial resistance is a growing global health concern, as many pathogen microorganisms have become resistant to traditional antimicrobial agents. This has led a urging need for the development and research new alternative therapies to combat against this resistant strains. Chitosan nanoparticles have shown to be a promising strategy to overcome this problem, due to their proven antibacterial activity, besides the possibility of carry some antimicrobial agents inside them to enhance the antimicrobial activity. Therefore, this article will summarize briefly the current state on chitosan nanoparticles research to overcome antimicrobial resistance.La resistencia a antimicrobianos es un problema creciente de salud pública mundial, pues cada vez hay más microorganismos patógenos resistentes a los agentes antimicrobianos tradicionales. Esto ha originado una creciente necesidad por investigar y desarrollar nuevas terapias para combatir a estas cepas resistentes. Las nanopartículas de quitosano se han convertido en una alternativa atractiva y prometedora para solucionar este problema, pues ya se ha demostrado su actividad contra bacterias multirresistentes. Además, como sistemas acarreadores de agentes antimicrobianos para liberación controlada, han demostrado ser una alternativa adecuada a la administración convencional de fármacos. Por tanto, en el presente artículo se resumirá brevemente el estado actual de la investigación en nanopartículas de quitosano aplicada en combatir la resistencia a antimicrobianos

Whole genome, transcriptome, smallRNAome and methylome profiling during tomato-geminivirus interaction

Contribución a congresoTomato Yellow Leaf Curl Virus (TYLCV) belongs to the Begomovirus genus and istransmitted by the whitefly Bemisia tabaci. With only seven viral proteins, TYLCV must create a proper environment for viral replication, transcription, and propagation. Behind the apparent simplicity of geminiviruses lies a complex network of molecular interactions with their host and their natural vector, which induces a wide variety of transcriptional, post-transcriptional and chromatin changes in the host. To understand this virus-host interaction at a genetic and epigenetic level, we carried out a global approach to generate the transcriptome, smallRNAome and methylome of the TYLCV-tomato interaction. Total RNA and DNA was extracted from tomato infected plants (three biological replicates) and analysed at 2, 7, 14 and 21-day postinfection (dpi). Analysis of the changes in host transcription during the infection and its correlation with changes in sRNA profiles (microRNA and phasiRNA) and DNA methylation patterns will be presented and discussed.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

The begomovirus Tomato leaf curl New Delhi virus is not seed-transmitted in melon

Transmission of plant viruses through seed can be one of the major factors contributing to long- distance dispersal through global trade of seeds and can have important ecological consequences for virus dissemination. Begomoviruses (genus Begomovirus, family Geminiviridae), and among them isolates of the species Tomato leaf curl New Delhi virus (ToLCNDV), cause significant yield losses in economically important crops worldwide. These viruses are horizontally transmitted in nature in a circulative and persistent manner by the whitefly Bemisia tabaci but in recent years several reports have raised the possibility of vertical transmission through seeds for some members of this genus. We have investigated the possible transmission by melon (Cucumis melo L.) seeds of a ToLCNDV isolate of the “Spain” strain, in three different melon cultivars (all susceptible to ToLCNDV). The presence of ToLCNDV in floral tissues and the detection of viral DNA in seeds reveals the seed-borne nature of this virus. However, grow-out studies conducted with the progeny of melon plants germinated from seeds collected from ToLCNDV-infected plants and evaluated at early (1 leaf) or at late (20 leaves) growth stages did not support the vertical transmission of ToLCNDV from seeds to the offspring.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tec

The begomovirus tomato leaf curl New Delhi virus is not seed-transmitted in melon

Transmission of plant viruses through seed can be one of the major factors contributing to longdistance dispersal through global trade of seeds and can have important ecological consequences for virus dissemination. Begomoviruses (genus Begomovirus, family Geminiviridae), and among them isolates of the species Tomato leaf curl New Delhi virus (ToLCNDV), cause significant yield losses in economically important crops worldwide. These viruses are horizontally transmitted in nature in a circulative and persistent manner by the whitefly Bemisia tabaci but in recent years several reports have raised the possibility of vertical transmission through seeds for some members of this genus. We have investigated the possible transmission by melon (Cucumis melo L.) seeds of a ToLCNDV isolate of the “Spain” strain, in three different melon cultivars (all susceptible to ToLCNDV). The presence of ToLCNDV in floral tissues and the detection of viral DNA in seeds reveals the seed-borne nature of this virus. However, grow-out studies conducted with the progeny of melon plants germinated from seeds collected from ToLCNDV-infected plants and evaluated at early (1 leaf) or at late (20 leaves) growth stages did not support the vertical transmission of ToLCNDV from seeds to the offspring.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Identification of a novel potassium channel (GiK) as a potential drug target in Giardia lamblia: Computational descriptions of binding sites

Background The protozoan Giardia lamblia is the causal agent of giardiasis, one of the main diarrheal infections worldwide. Drug resistance to common antigiardial agents and incidence of treatment failures have increased in recent years. Therefore, the search for new molecular targets for drugs against Giardia infection is essential. In protozoa, ionic channels have roles in their life cycle, growth, and stress response. Thus, they are promising targets for drug design. The strategy of ligand-protein docking has demonstrated a great potential in the discovery of new targets and structure-based drug design studies. Methods In this work, we identify and characterize a new potassium channel, GiK, in the genome of Giardia lamblia. Characterization was performed in silico. Because its crystallographic structure remains unresolved, homology modeling was used to construct the three-dimensional model for the pore domain of GiK. The docking virtual screening approach was employed to determine whether GiK is a good target for potassium channel blockers. Results The GiK sequence showed 24–50% identity and 50–90% positivity with 21 different types of potassium channels. The quality assessment and validation parameters indicated the reliability of the modeled structure of GiK. We identified 110 potassium channel blockers exhibiting high affinity toward GiK. A total of 39 of these drugs bind in three specific regions. Discussion The GiK pore signature sequence is related to the small conductance calcium-activated potassium channels (SKCa). The predicted binding of 110 potassium blockers to GiK makes this protein an attractive target for biological testing to evaluate its role in the life cycle of Giardia lamblia and potential candidate for the design of novel antigiardial drugs

Aprendizaje Servicio e Innovación Social desde la Universidad

El Aprendizaje Servicio es una estrategia docente necesaria para el desarrollo de iniciativas de emprendimiento e innovación social, que partan desde la Universidad, y que se puedan incorporar en las diferentes áreas de conocimiento de la UC

Vascular and root tip GPT2 expression mediates the PGI1-independent response of Arabidopsis to small microbial volatiles

Resumen del trabajo presentado en el XVI Meeting of Plant Molecular Biology, celebrado en Sevilla (España), del 14 al 16 de septiembre de 2022Microorganisms emit a plethora of volatile compounds (VCs) that promote plant growth and photosynthesis as well as strong developmental and metabolic changes. In Arabidopsis, the plastidial isoform of phosphoglucose isomerase PGI1 mediates photosynthesis, metabolism and development, probably due to its involvement in the synthesis of isoprenoid-derived signals in vascular tissues (Bahaji et al., 2015; Bahaji et al., 2018). Like in wild-type (WT) plants, microbial VCs promote growth and photosynthesis as well as starch and CK accumulation in PGI1-lacking pgi1-2 plants (Sánchez-López et al. 2016). A striking alteration in the transcriptome of leaves of small fungal VC-treated plants involves strong up-regulation of levels of transcripts of GPT2 (At1g61800), a gene that codes for a plastidial G6P/Pi transporter. We hypothesized that the PGI1-independent response to microbial volatile emissions involves GPT2 action. To test this hypothesis, we characterized responses of WT, GPT2-null gpt2-1, PGI1-null pgi1-2 and pgi1- 2gpt2-1 plants to small fungal VCs. In addition, we characterized responses of pgi1-2gpt2-1 plants expressing GPT2 under the control of a vascular tissue- and root tip-specific promoter to small fungal VCs. Results presented in this work provide evidence that, under conditions in which PGI1 activity is reduced, long-distance action of GPT2 plays an important role in the response of plants to small VCs through mechanisms involving resetting of the photosynthesis-related proteome in leaves and complex GPT2 regulation.This work was supported by the Ministerio de Ciencia e Innovación (MCIN) and Agencia Estatal de Investigación (AEI) / 10.13039/501100011033/ (grants BIO2016-78747-P, PID2019-104685GB-100 and PID2019-107657RB-C22) and the Ministry of Education, Youth and Sport of the Czech Republic and ERDF project entitled “Plants as a tool for sustainable global development” (No. CZ.02.1.01/0.0/0.0/16_019/0000827)