Protective effect of Garcinia against renal oxidative stress and biomarkers induced by high fat and sucrose diet

<p>Abstract</p> <p>Background</p> <p>Obesity became major health problem in the world, the objective of this work was to examine the effect of high sucrose and high fat diet to induce obesity on antioxidant defense system, biochemical changes in blood and tissue of control, non treated and treated groups by administration of Garcinia cambogia, and explore the mechanisms that link obesity with altered renal function</p> <p>Methods</p> <p>Rats were fed a standard control diet for 12 week (wk) or a diet containing 65% high sucrose (HSD) or 35% fat (HFD) for 8 wk and then HFD group divided into two groups for the following 4 wks. One group was given <it>Garcinia</it>+HFD, the second only high fat, Also the HSD divided into two groups, 1^stHSD+<it>Garcinia </it>and 2^ndHSD. Blood and renal, mesenteric, Perirenal and epididymal adipose tissues were collected for biochemical assays.</p> <p>Results</p> <p>HFD and HSD groups of rats showed a significant increase in feed intake, Body weight (BW) and body mass index (BMI). Also there were significant increases in weights of mesenteric, Perirenal and epididymal adipose tissues in HFD and HSD groups.</p> <p>HFD and HSD affect the kidney by increasing serum urea and creatinine levels and decreased level of nitric oxide (NO) and increased blood glucose, low density lipoproteins (LDL), triacylglycerol (TG), total cholesterol (TC) and malondialdehyde (MDA). Glucose 6-phosphate dehydrogenase (G6PD) activities were significantly decreased in HFD while there were significant increases in HSD and HSD+G groups p ≤ 0.05 compared with control. Moreover, renal catalase activities and MDA levels were significantly increased while NO level was lowered. These changes improved by <it>Garcinia </it>that decreased the oxidative stress biomarkers and increased NO level.</p> <p>There were significant positive correlations among BMI, kidney functions (Creatinine and urea), TG and Oxidative markers (renal MDA and catalase).</p> <p>Conclusions</p> <p>Rats fed a diet with HFD or HSD showed, hypertriglyceridemia, increased LDL production, increased oxidative stress and renal alteration. Moreover, suggesting association between lipid peroxidation, obesity and nephropathy, while <it>Garcinia </it>ameliorated the damaging effects of the HFD or HSD and decreased feed intake, MDA level and decreased oxidative stress in renal tissues.</p

The relation of high fat diet, metabolic disturbances and brain oxidative dysfunction: modulation by hydroxy citric acid

<p>Abstract</p> <p>Aims</p> <p>This study aimed to examine the effect of high fat diet (HFD) to modulate brain dysfunction, and understand the linkages between obesity, metabolic disturbances and the brain oxidative stress (BOS) dysfunction and modulation with hydroxyl citric acid of <it>G. Cambogia</it>.</p> <p>Methods</p> <p>Rats were divided into 3 groups; 1^stcontrol, maintained on standard normal rat chow diet, 2^ndHFD, maintained on high fat diet along 12 week and 3^rdHFD+G, administered <it>G. Cambogia </it>for 4 weeks and each group include 8 rats. Blood, brain and abdominal fat were collected for biochemical measurements.</p> <p>Results</p> <p>HFD group showed significant increase in energy intake, final BW and BW gain. Also significant increase in weight of abdominal fat in HFD group. HFD induce metabolic disturbance through increasing the lipid profile (LDL, TG, TC), γGT and α-amylase activity, uric acid level and hyperglycemia, while decreasing creatine kinase (CK) activity.</p> <p>These changes associated with lowering in brain nitric oxide (NO) level and rising in serum butyrylcholinesterase (BChE), brain catalase activity and MDA levels as oxidative stress markers. These alterations improved by <it>G. Cambogia </it>that decrease BOS and increased NO level.</p> <p>Conclusions</p> <p>Rats fed HFD showed, metabolic disturbances produce hyperglycemia, hypertriglyceridemia, hypercholesterolemia and increased LDL associated with increased BOS. Involvement of BuChE, NO and oxidative stress associated with metabolic disturbances in the pathophysiological progression in brain, suggesting association between obesity, metabolic disorders and brain alteration while, using <it>G. Cambogia</it>, ameliorate the damaging effects of the HFD via lowering feed intake and BOS.</p

Studi Fasies Formasi Endapan Danau untuk Menentukan Lingkungan Pengendapan Danau Limboto

The research area located in the lowland zone and the corrugated hilly zone which is dominated by sedimentary rocks. Therefore, this study aims to determine the geological features of the research area and the study of depositional environmental facies of the Lake Deposits Formation as an analysis of the depositional environment of Limboto Lake. The research method used is the surface geological mapping, measuring a section of stratigraphic and laboratory analysis. Field data consists of stratigraphical features of the research area. Laboratory analysis consists of petrography and stratigraphy-sedimentology analysis. It can be concluded facies and stratigraphy of research from older to youngest, consists of clastic limestones unit formed in Late Miocene-late Early Pliocene age, reef limestones formed in Late Miocene-early Late Pliocene age, sandstones unit formed in Late Pliocene-Pleistocene age, clay unit, and sandy clay unit are deposited in Holocene. Overall the study site consisted of marine, transitional and terrestrial deposition environment with Lake Deposits Formation facies is lake margin clastic deposits and meandering-stream environment deposits

Immunohistochemical Expression of “L1 cell Adhesion Molecule†in Endometrial Carcinomas

BACKGROUND: Endometrial cancer is the most common cancer of the female genital tract. No effective biomarkers currently exist to allow for an efficient risk classification of endometrial carcinoma or to direct treatment (adjuvant radiation and/or chemotherapy) or to triage pelvic and para-aortic lymphadenectomy. L1 cell adhesion molecule (L1CAM) a transmembrane protein of the immunoglobulin family that has been implicated in promoting tumor cell proliferation, migration, invasion, and metastasis became an attractive candidate as a potential biomarker in endometrial carcinoma and potential therapeutic target in high-risk groups. OBJECTIVES: Evaluation of L1CAM expression in endometrial carcinoma and correlation of this expression with various pathological parameters. MATERIALS AND METHODS: Immunohistochemical staining for L1CAM was performed on paraffin-embedded sections of 80 cases of endometrial carcinomas that underwent total hysterectomy with bilateral salpingo-oophorectomy. Expression of L1CAM in &gt;10% of tumor cells was interpreted as positive. RESULTS: L1CAM expression was detected in 22.5% of cases and showed statistically significant correlation with non-endometrioid histological type, high grade, high FIGO stage, high pathological (T) stage, cervical involvement, nodal metastasis, lymphovascular space invasion, and high-risk tumor according to the European Society for Medical Oncology system for risk stratification (p &lt; 0.05). CONCLUSION: The high rate of L1CAM expression in high-risk endometrial carcinomas suggests that L1CAM represents a potential marker for the identification of patients needing closer follow-up and aggressive treatment. In addition, its potential role as a therapeutic target for high-risk endometrial cancer seems promising

Arabic gum acacia improves diabetic peripheral neuropathy in rats: a biochemical and histopathological evidence

Background: Diabetic peripheral neuropathy (DPN) is a frequent complication of diabetes mellitus and unfortunately, its present therapeutic alternatives are exceptionally poor. Objectives of this study was to assess the antidiabetic, antioxidant and hypolipidemic action of Gum Arabic (GA) and its role in promoting the functional recovery from diabetic neuropathy developed in in an experimental model of diabetic neuropathy.Methods: Sixty adult male Sprague-Dawley rats were utilized and randomly assigned into six groups (n= 10); control, Arabic gum-treated, untreated diabetic, diabetic received metformin, diabetic received metformin and B12 vitamin and diabetic received metformin, B12 vitamin and AG. Locomotor activity and hyperalgesia were assed at the end of the study. Fasting and two hours post-prandial blood glucose, serum insulin levels, lipid Profile, oxidants/antioxidants parameters were assessed in the blood. Sciatic nerve was assessed histopathologically.Results: The locomotor activity of the untreated diabetic rats was significantly (p<0.001) reduced compared to the control group while it was significantly increased in all treated groups. The lipid profile and Malondialdehyde were significantly improved in all treated groups. Levels of CAT, GSH, SOD, GPx were significantly decreased in untreated diabetic group compared to the control while they were significantly increased in all treated groups compared to the untreated diabetic group. Sciatic nerve fibers of untreated diabetic rats showed degenerated axons with dilated myelin sheaths and degenerated Schwann cells. The nerve had significantly fewer fiber compared to the control. These changes were alleviated in all the treated groups specifically that received metformin, vitamin B12 and GA.Conclusions: It could be concluded that Arabic gum had hypoglycemic, antioxidant and hypolipidemic activity and had a protective effect on diabetic neuropathy. Based on this it is recommended that human clinical trials are necessary to prove this therapeutic effect

Euphotic Depth Zone Variation in Peninsular Malaysia Maritime

This study is conducted in Peninsular Malaysia maritime to investigate the euphotic zone depth (Zeu) variation and the possible suspended matter that may contribute to the variation. The Zeu data were acquired from the MODIS Aqua satellite from November 2002 to September 2013. The result shows that the Zeu along the Malaysia maritime are highly seasonal-dependent. The lowest Zeu values are observed during the northeast monsoon season (NEMS) in the east coast Peninsular Malaysia and during the southwest monsoon season (SWMS) for the west coast area. Chlorophyll- a (Chl-a) and colored dissolved organic matter (CDOM) are found to be the contributing factors for the coastal line and open water area. While, sediment only contributes to the area located along the coastal line where lower Zeu values are observed

A Comparison between Ratio and Gradient Technique in Discriminating Cirrus Clouds from Tropospheric Aerosols over Water in MODIS Data

This study aims to compare between ratio technique (RT) and gradient technique (GT) to distinguish cirrus cloud from tropospheric aerosol over water in MODIS data. Both techniques make use of 1.375 µm and 1.240 µm band and are applied to five different scenes. The outcomes from both techniques are compared using an error matrix in which revealing that the GT has a very high agreement with RT in distinguishing cirrus cloud from tropospheric aerosol in MODIS data

Studies on Some Parasitic Diseases in Oreochromis niloticus Fish Hatchery with Emphasis to Life Stages

This study was conducted on 210 Oreochromis niloticus (O. niloticus) of different life stages including (100 fry, 100 fingerlings and 10 broodstocks) obtained from a private fish hatchery at Kafer El-Sheikh Governorate, Egypt; during August 2014. The hatchery complains 30% mortality among fry and fingerlings while no mortalities was recorded among broodstocks. Parasitological examination revealed heavy infestation with Triochodina species (sp.) in all examined life stages at a prevalence rate 100%. In addition, Gyrodactylus sp. was recorded in gills of fry, fingerlings and broodstocks at a rate of 5, 12, and 10 %, respectively. Kidneys and gills of all examined life stages showed heavy infestations with Myxosporean sp., with 100 % prevalence rate. Haemogregarina sp. was described in the blood of fingerlings and gill tissues of broodstocks. Additionally, Encysted metacerceria was observed in gills of broodstocks. The recovered parasites were demonstrated hisopathologically in the gill and kidney tissues of the examined fish. The histopathological examination revealed that the infested gills exhibited serious lesions such as hyperplasia and hypertrophy of the lining epithelial cells of the gill filaments, fusion and necrosis of secondary lamellae and vasodilatation. The lining epithelium of the renal tubules showed degenerative and necrotic changes with the presence of various developmental stages of myxosporidia. In conclusion, fry and fingerlings exhibited high mortalities, while no mortality was recorded among broodstocks, regardless the intensity of infestation and severity of pathological alterations which was intense in broodstocks

TOPICAL BENZOPHENONE-3 MICROEMULSION-BASED GELS: PREPARATION, EVALUATION AND DETERMINATION OF MICROBIOLOGICAL UV BLOCKING ACTIVITY

Objective: Microemulsions (MEs) have been developed as active vehicles for sunscreens being physical sunscreens on their own.The aim of this study is to incorporate Benzophenone-3 (BZ-3), a chemical sunscreening agent into MEs resulting in a synergistic effect on its protective characteristics. Methods: Screening of BZ-3 in different oils, surfactants and cosurfactants available for topical delivery was carried out. A full factorial study design (3.22) was adopted to study the effect of three independent variables namely;BZ-3, oil, and S/CoSmix(1:1) concentrations on the in-vitro SPF value of the prepared ME gel formulae. Results: Formulae having SPF&gt;30 were further evaluated regarding in-vitro permeation test. Finally, an in vitro microbiological assay was adopted to compare the survival percent of E.coli against UV exposure. Formula F11IPM ME gel consisting of 3% BZ-3 and 10% Isopropyl myristate as oil together with 60% w/w Tween80/Cremophore RH40 mix (1:1)as surfactant and cosurfactant respectively was chosen as the optimum formula having an in vitro SPF of 33.15±0.91,lowest permeation of 192±16.13µg/cm2 after eight hours and microbiological UV blocking activity of 88±5.84%which is not significantly different from the standard Spectra Ban plus (SPF=30) having a survival percent of 80±3.04%. Conclusion: This study illustrated the potential use of microemulsified BZ-3 delivery system to improve the SPF of BZ-3

Studi Fasies Formasi Endapan Danau Untuk Menentukan Lingkungan Pengendapan Danau Limboto

The research area located in the lowland zone and the corrugated hilly zone which is dominated by sedimentary rocks. Therefore, this study aims to determine the geological features of the research area and the study of depositional environmental facies of the Lake Deposits Formation as an analysis of the depositional environment of Limboto Lake. The research method used is the surface geological mapping, measuring a section of stratigraphic and laboratory analysis. Field data consists of stratigraphical features of the research area. Laboratory analysis consists of petrography and stratigraphy-sedimentology analysis. It can be concluded facies and stratigraphy of research from older to youngest, consists of clastic limestones unit formed in Late Miocene-late Early Pliocene age, reef limestones formed in Late Miocene-early Late Pliocene age, sandstones unit formed in Late Pliocene-Pleistocene age, clay unit, and sandy clay unit are deposited in Holocene. Overall the study site consisted of marine, transitional and terrestrial deposition environment with Lake Deposits Formation facies is lake margin clastic deposits and meandering-stream environment deposits