Indução de embriogénese somática em Pinus elliottii e o hibrido

Mestrado em Biologia Molecular e CelularDada a importância comercial de Pinus elliottii e do híbrido Pinus elliottii x Pinus caribaea, e a necessidade de desenvolver uma bateria de protocolos de cultura in vitro para aplicação em programas de (micro) propagação e em programas de investigação em geral (eg, fisiologia, biologia molecular, etc), estabeleceu-se como objetivo principal estudar o efeito de vários fatores (endógenos e exógenos) na indução de: a) tecido caloso (importante em estudos fundamentais de fisiologia, manutenção de germoplasma, morfogénese, etc); b) tecido caloso embriogénico (importante para a regeneração de plantas por ES, estudos de criopreservação, etc). Neste sentido, embriões zigóticos maduros (EZM) e cotilédones foram sujeitos a diferentes concentrações de reguladores de crescimento (2 mg/L de 2,4-D; 2 mg/L de NAA e 0,02 mg/L de TDZ). Os primeiros sinais de callus surgiram ao fim de 15 dias. A concentração de reguladores de crescimento mais eficiente em EZM foi 2 mg/L de 2,4-D com uma taxa de indução de calli de 85% e em cotilédones foi 2 mg/L de 2,4-D com 55% de indução de calli. EZM foram sujeitos a diferentes concentrações de reguladores de crescimento (2,4 mg/L 2,4-D + 1 mg/L 24-epiBr; 8 mg/L 2,4-D + 4 mg/L BAP); fontes de carbono (30 g/L de sacarose e 30 g/L de maltose) e diferentes fatores de stresse, nomeadamente calor (80ºC), frio (-15ºC), ácido salicílico (AS) (1 mg/L), prolina (250 mg/L), peróxido de hidrogénio (H2O2) (9 μL/L) e putrescina (2 mg/L) com o objetivo de testar o seu potencial na indução de callus. A combinação 2,4-D + 24-epiBr resultou em 10 % de explantes com calli, e a 2,4-D + BAP representou 30 %. Os meios com sacarose e maltose representaram, igualmente, 20% de explantes com calli. O frio induziu callus em 100% dos explantes contrariamente ao calor que apenas produziu 5 %. Em resposta ao AS, prolina, H2O2 e putrescina a indução de callus foi de 93%, 95%, 90% e 100%, respetivamente. As condições que levaram a maior produção de callus foram o frio e a putrescina. Estes resultados suportam a possível indução de callus nestas duas coníferas, e suportam dados preliminares no sentido da otimização de protocolos eficazes para a ES.Pinus elliottii and the hybrid Pinus elliottii x Pinus caribaea have significant commercial importance. Therefore it urges the need to develop a battery of in vitro culture protocols for (micro)propagation and for general research (eg, physiology, molecular biology, etc.) of these conifers. The main objective of this study was to analyze the effect of various endogenous and exogenous factors on the induction of: a) callus tissue (important in fundamental studies of physiology, maintenance of germplasm, morphogenesis, etc.); b) embryogenic callus (important for plant regeneration by SE, cryopreservation studies, etc). For that, mature zygotic embryos (MZE), and the cotyledons were subjected to different concentrations of growth regulators (2 mg/L 2,4-D, 2 mg/L NAA and 0.02 mg/L TDZ). The first signs of callus appeared after 15 days. The most efficient concentration of growth regulators in MZE was 2 mg/L 2,4-D at a rate of induction of calli of 85% and cotyledons was 2 mg/L 2,4-D with 55% induction of calli. MZE were subjected to different concentrations of growth regulators (2.4 mg/L 2,4-D + 1 mg/L 24-epiBr , 8 mg/L 2,4-D + 4 mg/L BAP); carbon source (30 g/L sucrose and 30 g/L maltose) and different stress factors, in particular heat (80 °C), cold (-15 °C), salicyli c acid (SA) (1 mg/L), proline (250 mg/L) hydrogen peroxide (H2O2) (9 μL/L) and putrescine (2 mg/L) in order to test its potential in inducing callus. The combination 2,4-D + 24 epiBr resulted in 10% of explants with calli and 2,4-D + BAP represented 30%. The media containing sucrose and maltose accounted also 20% of explants with calli. The cold induced callus from explants of 100% as opposed to heat produced only 5%. In response to SA, proline, putrescine and H2O2 induced callus was 93%, 95%, 90% and 100%, respectively. The conditions that led to higher production of callus were cold and putrescine. These results support the induction of callus in these two conifers, and support preliminary data towards the optimization of protocols for efficient SE

Enhancing the intercultural effectiveness of exchange programmes: formal and non-formal educational interventions

This paper examines how the addition of intercultural interventions carried out throughout European credit-bearing exchange programmes can enhance sojourners’ development of intercultural competencies, and it explores how both formal and non-formal pedagogical interventions may be designed and implemented. Such interventions were conducted at a Portuguese university with 31 sojourners throughout one academic year, and their impact was assessed using a mixed methods research design. Sojourners included incoming students of the exchange programmes Campus Europae and Erasmus, as well as highly skilled immigrants. Findings confirm the positive impact of interventions on the development of intercultural competencies and, in turn, their contribution to internationalisation efforts. Implications for further research suggest a need to increase interventions and to develop a systematic approach for fostering intercultural competencies throughout the study abroad cycle

The impact of the COVID-19 pandemic on people with lived experience of mental illness integrated into community-based psychosocial rehabilitation structures in Portugal

To analyze the prevalence and impact of SARS-CoV-2 infection in people with lived experience of mental illness integrated into community-based psychosocial rehabilitation structures in Portugal. One hundred and thirty-nine people with lived experience of mental illness integrated into community-based psychosocial rehabilitation structures in Portugal answered an online survey that included dimensions related to COVID-19 pandemic prevalence, routine/lifestyle, social support, access to health care, mental health and well-being during the pandemic and confinement, and life satisfaction and postpandemic future expectations. The results point to a low prevalence of SARS-CoV-2 infections in this sample. High levels of resilience and mental well-being were identified in the individuals. We also found that participants were satisfied with the social support during this phase and their routine/lifestyle. The study showed that the COVID-19 pandemic seems not to have had a significant negative impact on people with experience of mental illness integrated into community-based psychosocial rehabilitation structures in Portugal. However, more research in this field should be done in the future.info:eu-repo/semantics/publishedVersio

A closer look at the determinologisation process

UIDB/03213/2020 UIDP/03213/2020This paper arises within the current communication urgency experienced through-out the pandemic. From its onset, several new lexical units have permeated the over-all media discourse, as well as social media and other channels. These units conveyinformation to the public regarding the ‘severe acute respiratory syndrome’ namely COVID-19. In addition to its worldwide impact healthwise, the pandemic generates noteworthy influence in the linguistic landscape, and as a result, a significant number of neologisms have emerged. Within the scope of our ongoing research, we identify the neologisms in European Portuguese that are related to the term COVID-19 via form or meaning. However, not all the new lexical units identified in our corpus containing COVID-19 in its formation can unequivocally be regarded as neoterms (terminological neologisms). Accordingly, this article aims not only to reflect on thedistinction between neologism and neoterm but also to explore the determinologisa-tion process that several of these new lexical units experience.publishersversionpublishe

LSP in theory and practice

UIDB/03213/2020 UIDP/03213/2020Book of abstracts and Program of the 23rd International Conference on Languages for Specific Purposes (LSP 2022: Shaping knowledge through language: LSP in theory and practice), which took place at NOVA University Lisbon, Portugal (CAN – Colégio Almada Negreiros, 1099 – 032 Lisboa).publishersversionpublishe

Neurokinin-1 receptor, a new modulator of lymphangiogenesis in obese-asthma phenotype

Aims Obesity and asthma are widely prevalent and associated disorders. Recent studies of our group revealed that Substance P (SP) is involved in pathophysiology of obese-asthma phenotype in mice through its selective NK1 receptor (NK1-R). Lymphangiogenesis is impaired in asthma and obesity, and SP activates contractile and inflammatory pathways in lymphatics. Our aim was to study whether NK1-R expression was involved in lymphangiogenesis on visceral (VAT) and subcutaneous (SAT) adipose tissues and in the lungs, in obese-allergen sensitized mice. Main methods Diet-induced obese and ovalbumin (OVA)-sensitized Balb/c mice were treated with a selective NK1-R antagonist (CJ 12,255, Pfizer Inc., USA) or placebo. Lymphatic structures (LYVE-1 +) and NK1-R expression were analyzed by immunohistochemistry. A semi-quantitative score methodology was used for NK1-R expression. Key findings Obesity and allergen-sensitization together increased the number of LYVE-1 + lymphatics in VAT and decreased it in SAT and lungs. NK1-R was mainly expressed on adipocyte membranes of VAT, blood vessel areas of SAT, and in lung epithelium. Obesity and allergen-sensitization combined increased the expression of NK1-R in VAT, SAT and lungs. NK1-R antagonist treatment reversed the effects observed in lymphangiogenesis in those tissues. Significance The obese-asthma phenotype in mice is accompanied by increased expression of NK1-R on adipose tissues and lung epithelium, reflecting that SP released during inflammation may act directly on these tissues. Blocking NK1-R affects lymphangiogenesis, implying a role of SP, with opposite physiological consequences in VAT, and in SAT and lungs. Our results provide a clue for a novel SP role in the obese-asthma phenotype

Interlinking Lexicographic Data in the MORDigital Project

UIDB/03213/2020 UIDP/03213/2020publishersversionpublishe

Simulação discreta em 3D de uma linha de embalagem de carretéis MIG/MAG usando o software Simio / Discrete 3D simulation of a MIG / MAG reel packaging line using Simio software

A Simulação 3D é uma das ferramentas da Indústria 4.0. Este trabalho apresenta a modelagem e simulação 3D de uma linha de embalagem de carretéis de arames de solda MIG/MAG em uma empresa localizada em Contagem - MG. O sistema foi simulado usando o software Simio. O software permitiu a modelagem tridimensional para uma melhor compreensão dos processos da linha. A análise dos resultados mostrou congestionamentos localizados em um braço robótico e uma embaladora de carretéis. Foram feitas experimentações para eliminação dos gargalos, concluindo-se que é necessário aumentar o número de equipamentos alterando-se o “layout” do setor

Simulação em 3D de uma linha de embalagem de carretéis MIG/MAG usando o software Simio / 3D simulation of a MIG / MAG spool packaging line using Simio software

Este trabalho apresenta a modelagem e simulação de uma linha de embalagem de carretéis de arames de solda MIG/MAG em uma empresa localizada em Contagem - MG. O sistema foi simulado usando o software Simio. O software permitiu a modelagem tridimensional para uma melhor compreensão dos processos da linha. A análise dos resultados mostrou o congestionamento da linha que estavam localizados em um braço robótico e uma embaladora de carretéis. Foram feitas experimentações para eliminação dos gargalos, concluindo-se que é necessário aumentar o número de equipamentos alterando-se o “layout” do setor

The advent of a new lexicographical portuguese project

UID/LIN/03213/2013MORDigital is a newly funded Portuguese lexicographic project that aims to produce high-quality and searchable digital versions of the first three editions (1789; 1813; 1823) of the Diccionario da Lingua Portugueza by António de Morais Silva, preserving and making accessible this important work of European heritage. This paper will describe the current state of the art, the project, its objectives and the methodology proposed, the latter of which is based on a rigorous linguistic analysis and will also include steps necessary for the ontologisation of knowledge contained in and relating to the text. A section will be dedicated to the various investigation domains of the project description. The output of the project will be made available via a dedicated platform.publishersversionpublishe