A Novel Approach for Speech to Text Recognition System Using Hidden Markov Model

Speech recognition is the application of sophisticated algorithms which involve the transforming of the human voice to text. Speech identification is essential as it utilizes by several biometric identification systems and voice-controlled automation systems. Variations in recording equipment, speakers, situations, and environments make speech recognition a tough undertaking. Three major phases comprise speech recognition: speech pre-processing, feature extraction, and speech categorization. This work presents a comprehensive study with the objectives of comprehending, analyzing, and enhancing these models and approaches, such as Hidden Markov Models and Artificial Neural Networks, employed in the voice recognition system for feature extraction and classification

Lean Implementation on Indian manufacturing firm

In today’s market every manufacturing industry is trying to implement ‘Lean’ in its operations. This emergent need of reducing waste and getting efficient production had created a boom for the Lean Production (LP). Many people from corporate firms and management associates want a productive tool for achieving this task. Value Stream Mapping (VSM) is the solution to such emerging need, it identifies the source of waste practices and tries to scale down them analytically. VSM in this way measures all the value and non-value added processes, to evaluate the origin of wastes, their effect on different operation of industry and the processes in between. In this paper we have implemented VSM technique on a small scale industry to showcase its effect on cost of production, production lead time and the developing some procedure for reduction of root cause of this loss. Considering the current processes of the industries operations Current State map is developed to show how actual production is taking place at the industry before implementing any lean procedure. A Future State Map is finally developed considering the lean behaviours to reduce the waste production and to increase its productivity. This is inspected along with its takt time calculation. Thus, by curbing these wasteful practices we will show how manufacturing performance of the company can be upgraded by employment of VSM

Massively parallel profiling and predictive modeling of the outcomes of CRISPR/Cas9-mediated double-strand break repair.

Non-homologous end-joining (NHEJ) plays an important role in double-strand break (DSB) repair of DNA. Recent studies have shown that the error patterns of NHEJ are strongly biased by sequence context, but these studies were based on relatively few templates. To investigate this more thoroughly, we systematically profiled ∼1.16 million independent mutational events resulting from CRISPR/Cas9-mediated cleavage and NHEJ-mediated DSB repair of 6872 synthetic target sequences, introduced into a human cell line via lentiviral infection. We find that: (i) insertions are dominated by 1 bp events templated by sequence immediately upstream of the cleavage site, (ii) deletions are predominantly associated with microhomology and (iii) targets exhibit variable but reproducible diversity with respect to the number and relative frequency of the mutational outcomes to which they give rise. From these data, we trained a model that uses local sequence context to predict the distribution of mutational outcomes. Exploiting the bias of NHEJ outcomes towards microhomology mediated events, we demonstrate the programming of deletion patterns by introducing microhomology to specific locations in the vicinity of the DSB site. We anticipate that our results will inform investigations of DSB repair mechanisms as well as the design of CRISPR/Cas9 experiments for diverse applications including genome-wide screens, gene therapy, lineage tracing and molecular recording

PaCo: Probability-based Path Confidence Prediction

Coordinated Science Laboratory was formerly known as Control Systems LaboratoryNational Science Foundation / CCR-042971Gigascale Systems Research Cente

Hemoglobin receptor in Leishmania is a hexokinase located in the flagellar pocket

Hb endocytosis in Leishmania is mediated through a 46-kDa protein located in the flagellar pocket. To understand the nature of the Hb receptor (HbR), we have purified the 46-kDa protein to homogeneity from Leishmania promastigote membrane. Purified HbR specifically binds Hb. The gene for HbR was cloned, and sequence analysis of the full-length HbR gene indicates the presence of hexokinase (HK) signature sequences, ATP-binding domain, and PTS-II motif. Four lines of evidence indicate that HbR in Leishmania is a hexokinase: 1) the recombinant HbR binds Hb, and the Hb-binding domain resides in the N terminus of the protein; 2) recombinant proteins and cell lysate prepared from HbR-overexpressing Leishmania promastigotes show enhanced HK activity in comparison with untransfected cells; 3) immunolocalization studies using antibodies against the N-terminal fragment (Ld-HbR-ΔC) of Ld-HbR indicate that this protein is located in the flagellar pocket of Leishmania; and 4) binding and uptake of 125I-Hb by Leishmania is significantly inhibited by anti-Ld-HbR-ΔC antibody and Ld-HbR-ΔC, respectively. Taken together, these results indicate that HK present in the flagellar pocket of Leishmania is involved in Hb endocytosis

Comparison of Conventional Percutaneous Nephrolithotomy and Endoscopic Combined Intrarenal Surgery with Respect to Efficacy and Safety in Complex Renal Stone

When PCNL and RIRS alone are unable to completely clear complex renal calculi, a combination of the two modalities was tested. Endoscopically Combined Intra Renal Surgery (ECIRS) is name given to this procedure afterwards. It excludes the drawbacks of multi-puncture PCNL and simultaneously provides much higher stone free rates. ECIRS is a relatively new tool in the arsenal of urologists, and as of right now, there isn't much information available in developing nations. Methods: A Comparative Observational study where 40 patients were split into two groups of 20, one for ECIRS and the other for prone PCNL. Both groups' surgical times, stone removal rates, potential complications, and other post-operative results were compared. Results: The majority of the patients (26) were men between the ages of 36 and 45, and both the group's age and gender were similar. The average calculus size was 2.43 cm for the ECIRS group and 2.60 cm for the prone PCNL group. Mean duration of the surgery was 85.24 & 88.12 min in ECIRS and prone PCNL group respectively. In the ECIRS group, the stone-free rate was considerably greater with lesser requirement of additional punctures and blood transfusions. More ancillary procedures prone PCNL patients' hospital stays were observed, as well. Post-operative S. urea and S. creatinine, fever, pain, post-operative complications were comparable. Conclusion: In view of the findings of the study, ECIRS seems to be a better option in comparison to prone PCNL in terms of effectiveness and safety. &nbsp

Anaesthetic Considerations in Simultaneous Management of Pulmonary and Hepatic Ruptured Hydatid Cyst: A Case Report

Administering anaesthesia in ruptured hydatid cyst of lung/liver has always been challenging. The major perioperative goal of management in such patients include protection of healthy lung, chance of anaphylaxis, deranged liver enzymes and coagulation profile, hepatic resection, postoperative management of bronchopleural fistula, blood loss and analgesic management of large incision. We report a case of successful management of a 40-year-old male with hepatic and pulmonary ruptured hydatid cyst with bronchopleural fistula under general anaesthesia (using double lumen tube) and thoracic epidural