Study of the genetic polymorphism of diploid wheat Triticum boeoticum Boiss. using SSR markers

Diploid wheat Triticum boeoticum Boiss. (genome constitution AA) is a promising source of new valu­able alleles for improving cultivated wheat species. Therefore, the evaluation of the intraspecies diversity of T. boeoticum and DNA fingerprinting of accessions of this species are topical tasks. In this paper, the ge­netic diversity of over 60 T. boeoticum accessions was studied using 11 SSR markers. The analysis revealed 83 alleles, 7.5 alleles per locus on the average. The values of expected (HE) and observed (HO) heterozygosity varied within 0.00–0.74 and 0.17–0.89, respectively, the average indices being HO = 0.13 and HE = 0.52. The PIC value for each locus was within 0.17–0.88, 0.49 on the average. Unique alleles were found in all loci studied. Cluster analysis allowed the accessions studied to be combined into five major groups. The distances between the groups varied from 0 to 1, pointing to a high level of genetic differences in the collection under study. On the base of PCoA, five major groups were formed and some correspondence with the dendrogram was detected. Summarizing the data of PCoA and cluster analysis, we noted a weak genetic differentiation in the studied collection of T. boeoticum. A correlation between the genetic distance and geographic origin was revealed only for accessions of diploid wheat T. boeoticum from Iran. The analysis of the T. boeoticum accessions studied showed a wide diversity for SSR loci. The results expand our knowledge and pro­vide additional information on the genetic structure of the collection and on the genetic diversity of T. boeoti­cum accessions studied

Adaptive potential of durum wheat (Triticum durum Desf.) varieties of Azerbaijan

Background. Studies into the adaptability of the genetic diversity of cultivated plants and their wild relatives to various environments are induced by global climate change. Introduction of adaptive wheat cultivars resistant to environmental stressors is the basis for stable harvests. This article presents the results of a research into the adaptive value of indigenous durum wheat varieties and improved cultivars released in different years in Azerbaijan.Materials and methods. The experiments were carried out in the foothills of Mountainous Shirvan under unsecured non-irrigation at Gobustan Experiment Station of the Research Institute of Crop Husbandry. In 2012–2014, contrasting in weather conditions, levels and structure of yield were analyzed. VIR’s guidelines were used to study 42 durum wheat varie ties, including 10 landraces. The years of study differed sharply in rainfall. Selyaninov’s hydrothermal coefficient was used to assess the conditions of growing seasons.Results. Mostly modern cultivars of the semi-intensive type were distinguished for a set of agrobiological traits. Adaptability coefficients (0.81–1.23) showed that the response of the studied varieties to unfavorable conditions was highly expressed. Indigenous landraces ‘Ag bughda’ and ‘Bozak’, old breeding varieties ‘Arandani’, ‘Ag bughda 13’, ‘Kahraba’ and ‘Mirbashir 50’, and new cultivars ‘Karagilchig 2’ and ‘Barakatli 95’ had the highest adaptability coefficients. The first of them demonstrated stable yield, and the latter two were also resistant to stressors.Conclusion. Durum wheat yields under the conditions of moderately continental climate in Mountainous Shirvan depended on the number and weight of grains per ear. The varieties identified for the best adaptability are recommended to be included in crosses to develop new plastic cultivars of durum wheat

Analysis of Structure Destroyed Metal after Diffusion Heat Treatment

It was accomplished research of the structure steel which carbonitriding and subsequent heat treatment was exposed for its cause's destruction to discover. For measure quality field of metal were used methods optical, appearing electronic microscopy and X-ray diffraction. Therefore one of the principal problems were research phase composition, grain and dislocation structure of a metal the gear teeth. Mechanism of rising hear cracks in the gear teeth on different stages her making and their trajectories of evolution were determined

Extractive Dearomatization of Naphthalane Oil Fraction with Ionic Liquid and -N-Methyl-2-Pyrrolidone

In the article are given an analysis of the results of researches carried out for the purpose of selective treatment of the Naphthalane oil fraction boiling at 260-3400C with ionic liquid (IL) -morfolinphormiate synthesized on the basis formic acid + morpholine and - N-methyl-2-pyrrolidon (N-MP) and  as an extractant. The aim is to remove poisonous components -  sulfur compounds and toxic carcinogenic polynuclear aromatic hydrocarbons in order to improve therapeutic properties of Naphthalane oil cut. For this purpose we have used extraction method. On the basis of the conducted researches the role of -N-methyl-2-pyrrolidone and IL-morpholinephormiatee in extraction conditions have been determined in selective treatment of Nafthalan oil, according to the results of spectral analysis residual amount of aromatic hydrocarbons decreased from 18.5% wt accordance to 2%, 1.5%. As well as the amount of sulphur decreased from 0.0354% to 0.010%,  0.011% and 0.019%.In the next stage the treated raffinates with N-methyl-2-pyrrolidone has been purified on silica. In a result amount of aromatic hydrocarbons drastically decrease to be 0.07% and 0.02%. As a result we aquire transparency and at the same time the percentage amount of aromatic hydrocarbons drastically decreased from 2% to 0,07%, 1.5%, to 0.02%. Raffinates obtained in a 2-4 stage dearomatization with N-MP and IL. Raffinate are measured on NMR, UV and IR spectral analysis

2-[2-(4-Methoxyphenyl)-4,5-diphenyl-1 H

In the title compound, C(24)H(22)N(2)O(2), the central imidazole ring makes dihedral angles of 49.45 (8), 88.94 (9) and 19.43 (8)° with the benzene ring and the two phenyl rings, respectively. The dihedral angle between the phenyl rings is 77.86 (9)°, and they form dihedral angles of 49.06 (9) and 67.31 (8)° with the benzene ring. In the crystal, mol­ecules are linked by O—H⋯N hydrogen bonds, forming chains along the b axis. These chains are connected by C—H⋯O hydrogen bonds, forming a two-dimensional network parallel to (100). In addition, C—H⋯π inter­actions are also observed. The terminal C and O atoms of the ethanol group are disordered over two sets of sites with an occupancy ratio of 0.801 (5):0.199 (5)

Simplified immunosuppressive and neuroprotective agents based on gracilin A

The architecture and bioactivity of natural products frequently serve as embarkation points for the exploration of biologically relevant chemical space. Total synthesis followed by derivative synthesis has historically enabled a deeper understanding of structure–activity relationships. However, synthetic strategies towards a natural product are not always guided by hypotheses regarding the structural features required for bioactivity. Here, we report an approach to natural product total synthesis that we term ‘pharmacophore-directed retrosynthesis’. A hypothesized, pharmacophore of a natural product is selected as an early synthetic target and this dictates the retrosynthetic analysis. In an ideal application, sequential increases in the structural complexity of this minimal structure enable development of a structure–activity relationship profile throughout the course of the total synthesis effort. This approach enables the identification of simpler congeners retaining bioactivity at a much earlier stage of a synthetic effort, as demonstrated here for the spongiane diterpenoid, gracilin A, leading to simplified derivatives with potent neuroprotective and immunosuppressive activityThe authors acknowledge support from the NIH (R37 GM052964 to D.R.), NSF (CHE1800411, to D.R.) the Robert A. Welch Foundation (AA-1280 to D.R.), FEDER co-funded grants from CONSELLERIA DE Cultura, EDUCACION e ordenación Universitaria Xunta de Galicia (2017 GRC GI-1682, ED431C 2017/01), CDTI and Technological Funds, supported by Ministerio de Economía, Industria y Competitividad (AGL2014- 58210-R, AGL2016-78728-R, AEI/FEDER, UE) (to L.M.B.), ISCIII/PI1/01830 (to A.A.) and RTC-2016-5507-2 and ITC-20161072, from EU POCTEP 0161-Nanoeaters-1-E-1, Interreg AlertoxNet EAPA-317-2016 and H2020 778069-EMERTOX (to L.M.B.) and from the European Union’s Seventh Framework Programme managed by the Research Executive Agency (FP7/2007-2013 under grant agreement 312184 PHARMASEA to L.M.B. and M.J.). N. Bhuvanesh and J. Reibenspies (Center for X-ray Analysis, TAMU) secured X-ray data and W. Russell (Laboratory for Biological Mass Spectrometry, TAMU) provided mass data. Correspondence and requests for materials should be directed to D. Romo (chemistry) and L. Botana (biology).S

ISSR Analysis of Variability of Cultivated Form and Varieties of Pomegranate (Punica granatum L.) from Azerbaijan

The article presents the results of a study of genetic polymorphism for the first time carried out on pomegranate varieties and forms of Azerbaijan origin using molecular markers. In total, 102 PCR fragments were identified, of which 80 were polymorphic. The high level of polymorphism (75.5%) and the rich genetic diversity were identified among the studied pomegranate collection. As a result of data analysis and on the basis of the values of the basic parameters (PIC, EMR, MI, RP, MRP) determining informativeness of markers, all 14 ISSR primers were suitable for genotyping pomegranate accessions. The most effective markers (UBC808, UBC811, UBC834, and UBC840) were identified among the set of primers tested. A dendrogram was constructed on the basis of the data obtained, which made it possible to group genotypes into 16 major clusters. The genetic similarity index ranged from 0.032 to 0.94. The study of the genetic relationship of different pomegranate varieties confirms the effectiveness of the ISSR method, which makes it possible to determine the level of genetic diversity, as well as to establish the relationship among the studied pomegranate accessions

Результаты лечения рабдомиосаркомы мочеполовой системы у детей. Собственный 15-летний опыт

Background. Despite significount successes in treatment of rhabdomyosarcoma of urogenital system in children there are unresolved questions of choise of optimal chemotherapys combinations, intensity of chemotherapy, volumes and terms of radiotherapy, tactics of treatment residual tumors in last 3 decades.The objective: show 15 years experience of treatment local and locally prevalent rhabdomyosarcoma urogenital system in children. The prognosis for children and adolescents with rhabdomyosarcoma has improved with refinements in multi-modal therapy.Materials and methods. In reseach are included 86 patients with a median age of 8.4 (0.7–17) with a local genitourinary rhabdomyosarcoma, treated in N.N. Blokhin National Medical Research Centre of Oncology from 2000 to 2016. All patients were treated in different riskadopted clinical protocol included chemotherapy and radiotherapy (IRS, SIOP, CWS and local protocol DORMS-6).Results. A 10-year overall survival and disease-free survival rates were 76 and 72 % in the entire group rhabdomyosarcoma patients, respectively.Conclusion. The effectiveness of the risk-adopted strategy in the genitourinary rhabdomyosarcoma treatment as well as the need of new approaches and in the cases of residual viable tumor after induction chemotherapy was demonstrated. Введение. Несмотря на существенные успехи в лечении рабдомиосаркомы мочеполовой системы у детей, в последние 3 десятилетия нерешенными остаются вопросы выбора оптимальных комбинаций химиопрепаратов, интенсивности химиотерапии, объемов и сроков проведения лучевой терапии, тактики в отношении резидуальных опухолей.Цель исследования – представить 15‑летний опыт лечения локализованной и местно-распространенной рабдомиосаркомы мочеполовой системы у детей.Материалы и методы. В исследование включены 86 пациентов (средний возраст 8,4 года (0,7–17 лет)) с эмбриональной рабдомиосаркомой мочеполовой системы, получавших лечение в НМИЦ онкологии им. Н. Н. Блохина в период с 2000 по 2016 г. Лечение проводили согласно риск-адаптированным клиническим протоколам (IRS, SIOP, CWS-10, ДОРМС-6) на базе критериев TNM, IRSG и COG.Результаты. Десятилетняя общая выживаемость и безрецидивная выживаемость в общей группе составила 76 и 72 % соответственно. Заключение. Показаны эффективность риск-адаптированной терапии рабдомиосаркомы мочеполовой системы у детей, необходимость поиска новых подходов для пациентов с нерадикально удаленными и резидуальными опухолями

Восстановлены наблюдения на ледниках Актру на Алтае

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