189 research outputs found
Phylogeny and taxonomy of obscure genera of microfungi
The recently generated molecular phylogeny for the kingdom Fungi, on which a new classification scheme is based, still suffers from an under representation of numerous apparently asexual genera of microfungi. In an attempt to populate the Fungal Tree of Life, fresh samples of 10 obscure genera of hyphomycetes were collected. These fungi were subsequently established in culture, and subjected to DNA sequence analysis of the ITS and LSU nrRNA genes to resolve species and generic questions related to these obscure genera. Brycekendrickomyces (Herpotrichiellaceae) is introduced as a new genus similar to, but distinct from Haplographium and Lauriomyces. Chalastospora is shown to be a genus in the Pleosporales, with two new species, C. ellipsoidea and C. obclavata, to which Alternaria malorum is added as an additional taxon under its oldest epithet, C. gossypii. Cyphellophora eugeniae is newly described in Cyphellophora (Herpotrichiellaceae), and distinguished from other taxa in the genus. Dictyosporium is placed in the Pleosporales, with one new species, D. streliziae. The genus Edenia, which was recently introduced for a sterile endophytic fungus isolated in Mexico, is shown to be a hyphomycete (Pleosporales) forming a pyronellea-like synanamorph in culture. Thedgonia is shown not to represent an anamorph of Mycosphaerella, but to belong to the Helotiales. Trochophora, however, clustered basal to the Pseudocercospora complex in the Mycosphaerellaceae, as did Verrucisporota. Vonarxia, a rather forgotten genus of hyphomycetes, is shown to belong to the Herpotrichiellaceae and Xenostigmina is confirmed as synanamorph of Mycopappus, and is shown to be allied to Seifertia in the Pleosporales. Dichotomous keys are provided for species in the various genera treated. Furthermore, several families are shown to be polyphyletic within some orders, especially in the Capnodiales, Chaetothyriales and Pleosporales
Genera of diaporthalean coelomycetes associated with leaf spots of tree hosts
Four different genera of diaporthalean coelomycetous fungi associated with leaf spots of tree hosts are morphologically treated and phylogenetically compared based on the DNA sequence data of the large subunit nuclear ribosomal DNA gene (LSU) and the internal transcribed spacers and 5.8S rRNA gene of the nrDNA operon. These include two new Australian genera, namely Auratiopycnidiella, proposed for a leaf spotting fungus occurring on Tristaniopsis laurina in New South Wales, and Disculoides, proposed for two species occurring on leaf spots of Eucalyptus leaves in Victoria. Two new species are described in Aurantiosacculus, a hitherto monotypic genus associated with leaf spots of Eucalyptus in Australia, namely A. acutatus on E. viminalis, and A. eucalyptorum on E. globulus, both occurring in Tasmania. Lastly, an epitype specimen is designated for Erythrogloeum hymenaeae, the type species of the genus Erythrogloeum, and causal agent of a prominent leaf spot disease on Hymenaea courbaril in South America. All four genera are shown to be allied to Diaporthales, although only Aurantiosacculus (Cryphonectriaceae) could be resolved to family level, the rest being incertae sedis
Plant growth and leaf-spot severity on eucalypt at different CO2concentrations in the air
Species diversity in Pseudocercospora
SUPPLEMENTARY MATERIAL : TABLE S1. Metadata and GenBank accession numbers of
Pseudocercospora isolates included in the morphological and/or
phylogenetic analyses.
TABLE S2. Summary of phylogenetic information for the different
analyses in this study.
TABLE S3. Comparison of the phylogenetic position of strains
represented by only ITS in the sequence dataset.Species of Pseudocercospora are commonly associated with leaf and fruit spots on diverse plant hosts in
sub-tropical and tropical regions. Pseudocercospora spp. have mycosphaerella-like sexual morphs, but represent a
distinct genus in Mycosphaerellaceae (Mycosphaerellales, Dothideomycetes). The present study adds a further 29
novel species of Pseudocercospora from 413 host species representing 297 host genera occurring in 60 countries and
designates four epitypes and one lectotype for established names. This study recognises 329 species names, with
an additional 69 phylogenetic lineages remaining unnamed due to difficulty in being able to unambiguously apply
existing names to those lineages. To help elucidate the taxonomy of these species, a phylogenetic tree was generated
from multi-locus DNA sequence data of the internal transcribed spacers and intervening 5.8S nuclear nrRNA gene
(ITS), partial actin (actA), and partial translation elongation factor 1-alpha (tef1), as well as the partial DNA-directed
RNA polymerase II second largest subunit (rpb2) gene sequences. Novel species described in this study include those
from various countries as follows: Australia, Ps. acaciicola from leaf spots on Acacia sp., Ps. anopter from leaf spots
on Anopterus glandulosus, Ps. asplenii from leaf spots on Asplenium dimorphum, Ps. australiensis from leaf spots
on Eucalyptus gunnii, Ps. badjensis from leaf spots on Eucalyptus badjensis, Ps. erythrophloeicola from leaf spots on
Erythrophleum chlorostachys, Ps. grevilleae from leaf spots on Grevillea sp., Ps. lophostemonigena from leaf spots
on Lophostemon confertus, Ps. lophostemonis from leaf spots on Lophostemon lactifluus, Ps. paramacadamiae from
leaf spots on Macadamia integrifolia, Ps. persooniae from leaf spots on Persoonia sp., Ps. pultenaeae from leaf spots
on Pultenaea daphnoides, Ps. tristaniopsidis from leaf spots on Tristaniopsis collina, Ps. victoriae from leaf spots on
Eucalyptus globoidea. Brazil, Ps. musigena from leaf spots on Musa sp. China, Ps. lonicerae-japonicae from leaf spots
on Lonicera japonica, Ps. rubigena leaf spots on Rubus sp. France (Réunion), Ps. wingfieldii from leaf spots on Acacia
heterophylla. Malaysia, Ps. musarum from leaf spots on Musa sp. Netherlands, Ps. rhododendri from leaf spots on
Rhododendron sp. South Africa, Ps. balanitis from leaf spots on Balanites sp., Ps. dovyalidicola from leaf spots on
Dovyalis zeyheri, Ps. encephalarticola from leaf spots on Encephalartos sp. South Korea, Ps. grewiana from leaf spots
on Grewia biloba, Ps. parakaki from leaf spots on Diospyros kaki, Ps. pseudocydoniae from leaf spots on Chaenomeles
lagenaria, Ps. paracydoniae from leaf spots on Chaenomeles speciosa. Thailand, Ps. acerigena from leaf spots on Acer
sp., Ps. tectonigena from leaf spots on Tectona grandis. Epitypes are designated for Cercospora bonjeaneae-rectae,
Cercospora halleriae, Ps. eucleae, and an epitype as well as a lectotype for Ps. macadamiae. Results obtained in the
present study contribute to a better understanding of the host specificity and distribution in Pseudocercospora spp.,
many of which represent important pathogens of food or fibre crops, or organisms of quarantine concern.The European Union’s Horizon 2020 research and
innovation program (RISE) under the Marie Skłodowska-Curie grant
agreement No. 10100812, the
Dutch NWO Roadmap grant agreement No. 2020/ENW/00901156, the
National Natural Science Foundation of China and the China Scholarship Council.https://fuse-journal.orghj2024BiochemistryForestry and Agricultural Biotechnology Institute (FABI)GeneticsMicrobiology and Plant PathologySDG-15:Life on lan
Estudo comparativo de isolados de Colletotrichum graminicola através da análise eletroforética de padrões protéicos e isoenzimáticos
Meloidogyne and Pratylenchus species in sugarcane fields in the state of Alagoas, Brazil
Controle químico da queima de folhas e da mela de estacas de eucalipto, causadas por Rhizoctonia spp.
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