响应面法提取公石松总黄酮工艺优化研究

目的:采用响应面法优化公石松总黄酮微波提取工艺。方法:先摸索液料比、反应时间、微波功率、反应温度四个微波提取条件,然后依据Box-Benhnken试验设计拟用四因素三水平方法,以公石松总黄酮提取率为响应值进行响应面试验,最后对不同公石松样品的总黄酮进行对比分析。结果:得出最佳提取工艺条件为:液料比30∶1(ml·g-1)、反应时间6.72 min,微波功率699.99 W,反应温度90℃,公石松总黄酮的提取率可达到1.02%。结论:微波提取工艺优化后公石松总黄酮的提取率可达1.02%;公石松叶子中的总黄酮含量高于茎,成熟期的总黄酮含量高于未成熟期。福建省教育厅中青年教师教育科研项目(编号:JAT171141);;漳州卫生职业学院课题项目(编号:ZYZ201701

Hardware implementation of a point multiplication algorithm based on parallel scheduling

针对gf(2M)域椭圆曲线点乘算法计算速度慢,硬件实现成本高的问题,提出一种改进的并行调度算法以及专用的硬件调度结构。通过合并点乘算法中底层模乘、模逆及模平方算法,降低底层运算单元硬件成本。同时对点乘算法进行模乘和模平方两路并行调度分解,以提高运算速度。并针对点乘调度算法的特点,设计专用的硬件调度结构,以减少调度复杂性。STrATIXIII系列的fPgA原型实现结果表明,通过这些方式使点乘运算速度得到提高。AnimprovedparallelschedulingalgorithmandspecifichardwareschedulingstructureispresentedinthispaperforsolvingtheproblemoflowspeedandhighhardwarecostofpointmultiplicationalgorithmofellipticcurveoverGF(2m).Mergingtheunderlyingmodularmultiplication,modularinversionandmodularsquarealgorithmisusedtoreducethecostofhardwareimplementation.Thenaparallelschedulinghasbeenmadetopointmultiplicationalgorithmwithmodularmultiplicationandmodularsquareforincreasingcomputingspeed.Besides,specifichardwareschedulingstructureisdesignedtoreducethecomplexityaccordingtothefeatureofpointmultiplication.TheresultshowsthatpointmultiplicationmoduleintroducedbythispaperhasreachedanexcellentcostperformancebothonspeedandhardwareresourcesonFPGAofStratixIIIseries.国家自然科学基金项目(61274133); 福建省自然科学基金项目(2010J05143

氯仿至全氯代稠环芳烃的液相电弧合成

以石墨电极在惰性气氛中放电,已成功地合成了C_（60）等碳原子簇。Grosser等人将此反应在氰气与氯气氛中进行,获得了一系列“棒状分子”与全氯代芳烃。我们又将这样的放电反应进一步扩展至溶液体系,将产生原子团簇的物理方法与传统的化学合成方法结合起来。最近,我们分析和研究了在液态的氯仿中放电的产物,在其中先后分离出六氯代苯（Ⅰ）、全

固氮酶中的电子传递

提出改进的二步ＡＴＰ驱动的电子传递机理，对还原剂和ＭｇＡＴＰ都充足或其中有一种不充足的情况下固氮酶体系的ＥＰＲ信号变化作了合理的解释

Copper(Ⅱ) Ion Coordination-Controlled Twisted Intramolecular Charge Transfer Dual Fluorescence Emission

设计合成了2个分子内扭转电荷转移(TICT)荧光体(1和2),铜离子的配位作用可开关其双重荧光发射。2在乙腈/水溶液(1∶1,V/V)中的双重荧光发射随着铜离子的加入以类似电子转移机制“开-关“形式猝灭,而1在铜离子与其计量比为1之内的乙腈/水溶液(1∶1,V/V)中,其双重荧光发射随着铜离子的加入逐渐猝灭;在计量比之后其长波长的TICT荧光发射随着铜离子的加入逐渐增强。即1的TICT荧光发射以“开-关-开“的机制被铜离子诱导。同时还获得了铜离子与1形成的配合物的晶体结构以及配合物的荧光性质。1H nMr波谱滴定实验表明荧光体的电荷转移程度是影响TICT发射的主要因素,1是一个新的且其TICT发射可以被铜离子调制为“开-关-开“的TICT荧光体。Two twisted intramolecular charge transfer(TICT) fluorophores(1 and 2) were designed and synthesized to control the dual fluorescence emission by Cu2 +coordination.The dual fluorescence of 2 is quenched upon the addition of Cu2+ions in an "on-off" process similar to PET inhibition.The dual fluorescence of 1 exhibits emission quenching initially and then long-wavelength band(TICT band) emission enhancement with "on-off-on" signalling behaviour.The crystal structure of the Cu2+complex with 1 was also obtained, and its fluorescence properties were reported.1H NMR spectral titration results indicate that the degree of charge transfer is a crucial factor for the resulting TICT state emission.Fluorophore 1 is a new TICT compound in which TICT emission can be tuned by copper ions with a dramatic change via "on-off-on" signalling behaviour.CCDC: 899958, Cu2+-1.国家自然科学基金(No.21271150); 国家基础科学人才培养基金(No.J1310024)资助项

固氮酶及合成氨Fe催化剂中N_2的络合位

用乙烯为探针研究了固氮酶中Ｎ２的键合位。结果表明，乙烯不能与Ｎ２在固氮酶体系中相竞争。提出Ｎ２在固氮酶中的键合位很可能是蛋白键合ＦｅＭｏ－ｃｏ笼内６Ｆｅ位的μ６（η２，ε４）和３Ｆｅ＋１Ｍｏ位的μ４（η３，ε１）方式，而不是笼口２Ｆｅ位的μ２（η２）方式，在合成氨Ｆｅ催化剂中Ｎ２的络合方式可能是μ６（η３，ε３）

Tannin dynamics of Kandelia candel roots in different salinity culture

对不同盐度(0‰-40‰)栽培下秋茄根的总酚、可溶性缩合单宁、结合缩合单宁、总缩合单宁的含量进行测定,探讨盐度对单宁生产的效应.结果表明:(1)不同盐度下栽培60-135d,总酚含量在相同盐度条件下随栽培时间的延长呈现出先波动而后升高的趋势,其中总酚最高含量出现在高盐度,说明一定的高盐度胁迫可以促进总酚的合成;总缩合单宁与可溶性缩合单宁含量变化趋势相似,其中可溶性缩合单宁含量显著高于结合缩合单宁.(2)在15‰盐度下栽培75-135d,秋茄根不同部位的总酚、可溶性缩合单宁、结合缩合单宁和总缩合单宁含量有相似的变化趋势,即由根尖往远离根尖的部位呈下降趋势.Total phenolics(TP),extractable condensed tannins(ECT),bound condensed tannins(BCT),and total condensed tannins(TCT) contents of Kandelia candel roots in different salinity culture were determined.In addition,the effect of salinity on tannin production was studied.The results showed as follows:(1) During the period of different salinity culture(from 60 d to 135 d),TP content fluctuated with increasing salinity,with the highest occurring in high salinity.ECT and TCT contents fluctuated under different salinity conditions with a consistent pattern.ECT contents were significantly higher than BCT contents.(2) At culture salinity of 15‰,the distribution of tannins in different parts of roots in the same period from 75 d to 135 d followed the identical pattern: the closer the root tip was,the higher the TP,ECT,BCT,and TCT contents were.福建省自然科学基金资助项目(B0410006);; 国家自然科学基金资助项目(40376026

Sequential observation of rebound shock wave generated by collapse of vapor bubble in BOS system

The paper reports on a pressure measurement of underwater shock waves generated by collapse of a vapor bubble using the background-oriented schlieren (BOS) technique. In the BOS system, the vapor bubble is induced by an underwater electric discharge. To estimate background-element displacement induced by the underwater shock wave, a spatiotemporal derivative (STD) algorithm is applied in image processing. Furthermore, the accuracy of the STD algorithm is evaluated using the images obtained with our BOS system relative to the cross-correlation method. Subsequently, the pressure distributions are obtained by solving the Poisson equation and applying a filtered-back projection algorithm. An experiment to visualize the behaviors of the underwater shock waves is also carried out using the shadowgraph method. As a result, the pressure waveforms estimated by the BOS technique keep consistent with that in experimental profiles. The pressure attenuations behind the underwater shock wave are also in good agreements with the experimental measurements. It is expected that the BOS technique will probably overcome the problems associated with the conventional method of pressure measurement related to the collapse of microbubbles, and becomes a promising means of quantitatively measuring high-speed phenomena.</p

固氮酶中N_2和N_2O结合位的一种新的鉴定方法

采用ＧＣ和ＧＣ－ＦＴＩＲ法，研究了Ｎ２和Ｎ２Ｏ对固氮酶催化Ｃ２Ｄ２还原活性及产物二氘代乙烯的反式－／顺式－异构体比值的影响，结果表明，Ｎ２和Ｎ２Ｏ均抑制Ｃ２Ｄ２的还原作用，并引起反式－／顺式－１，２－二氘代乙烯比值的增加．这一变化规律，与根据Ｃ２Ｈ２还原被Ｎ２和Ｎ２Ｏ抑制只发生在固氮酶铁钼辅基（ＦｅＭｏｃｏ）的笼内的假设而近似计算的结果相一致．实验结果和理论计算之间的一致性支持Ｎ２和Ｎ２Ｏ结合在ＦｅＭｏｃｏ的笼内，并主要抑制在笼内的Ｃ２Ｄ２还原的看