微囊藻毒素-RR对烟草细胞活力及营养生理的影响

采用0.1,1.0,10.0μg/mL的微囊藻毒素-RR(MC-RR)处理烟草BY-2悬浮细胞,测定了细胞活力、细胞内蛋白质含量、可溶性糖含量、硝态氮含量及总磷含量,并且检测了酸性磷酸酶(ACP)的活力变化情况.结果表明,中、高浓度毒素处理细胞2d后,细胞活力及蛋白质含量与对照相比均显著下降.高浓度MC-RR处理降低了胞内可溶性糖的含量,暴露2d后仅为对照的45.57%;低浓度MC-RR处理在后期增加了胞内可溶性糖含量.高浓度毒素处理细胞4d后,细胞内硝态氮含量显著低于对照;中、低浓度毒素处理细胞7d后

多波长萤光分光光度法(二)——萤光黄、罗丹明6G和罗丹明B三组分混合物的测定

本文叙述了应用多波长萤光分光光度法测定人工样品中的萤光黄、罗丹明6G和罗丹明B_。于波长502nm直接测量萤光黄的萤光强度,罗丹明6G和罗丹明B均不干扰;罗丹明6G的测定波长λ_1为555nm,参比波长λ_2和λ_3为502nm和660nm,罗丹明B的测定波长λ_1选用585nm,参比波长λ_2、λ_4和λ_3相应为538nm、508nm和494nm,方法的相对误差一般小于6%。作者于前文叙述了多波长萤光分光光度法的原理,为了验证该原理的可靠性,特对萤光黄、罗丹明6G和罗丹明B人工混合样品进行实测。结果表明,方法原理可靠,实验操作和数据处理简便快速,三次测定平均偏差<3%

Modeling Influence of Temperature and Salinity on Growth Rate of Larva in Noble Scallop,Chlamys nobilis(Reeve) by Response Surface Methodology(RSM)

采用中心复合设计(CEnTrAl COMPOSITE dESIgn,CCd)的试验设计法研究了温度和盐度2个环境因子对华贵栉孔扇贝幼虫生长的影响。同时采用响应曲面分析法及基于响应曲面基础之上的满意度函数法确立了生长的二次回归方程,同时进行优化处理得到温度与盐度的最佳组合。结果表明,在华贵栉孔扇贝幼虫生长过程中温度与盐度在一定的范围内的互作效应不显著,其中温度效应较为明显。温度对壳长瞬时生长影响的一次项效应和二次项效应均达到显著水平(P<0.000 1)。盐度对壳长瞬时生长影响一次项效应显著(P<0.000 1),然而,盐度对壳长瞬时生长影响的二次项效应达到显著水平(P<0.000 1)。分析响应曲面法优化温度和盐度对华贵栉孔扇贝幼虫壳长瞬时生长率的影响,其结果显示最佳温度与盐度组合为25.33℃与29.42‰,此条件下瞬时生长率为7.91%,其满意度函数值达到85.57%。在生产实践中,严格控制温度和盐度范围,使其在最优值范围内,将会显著提高苗种生产效率。In this study,the combined effects of temperature and salinity on the instantaneous growth rate(IGR) of larva in noble scallop,Chlamys nobilis,was evaluated using the central composite orthogonal quadratic design and the response surface methodology.The results showed that the linear effects of temperature on IGR was statistically highly significant(P<0.000 1);the linear effects of salinity on IGR was significant(P<0.000 1).The interactions between temperature and salinity on IGR was not significant;the quadratic effects of temperature and salinity on the IGR was highly significant(P<0.000 1);temperature was more important in influencing growth of larvaof C.nobilis.By applying the simultaneous optimization technique,the optimum factor combination,i.e.,25.33 ℃ and 29.42‰ was found out,at which the optimal IGR(7.91%)arrived simultaneously,with the desirability value as high as 85.57 %.It is anticipated that application of the optimal temperature-salinity combination to practice would improve the productive efficiency of C.nobilis.国家自然科学基金(31160528); 国家海洋公益性行业科研专项(201405020-5); 海洋公益专项科研基金(201205021); 南海生物资源开发与利用协同创新中

去岩藻糖GPC3抗体增强NK92细胞对肝癌细胞的杀伤效果

目的去岩藻糖基化改造抗磷脂酰肌醇蛋白聚糖3(Glypican-3,GPC3)抗体GC33,并研究该抗体依赖的NK92细胞对肝癌细胞的杀伤效果。方法以岩藻糖基转移酶8(fucosyltransferase 8,Fut8)缺陷的中国仓鼠卵巢癌细胞(Chinese hamster ovary,CHO)表达去岩藻糖基化的GC33抗体,以野生型CHO细胞表达的GC33作为对照,通过梯度酶联免疫反应检测其与GPC3及FC gama receptorⅢa(FCGRⅢa)的结合活性;以NK92细胞作为效应细胞,检测去岩藻糖基化GC33抗体依赖的NK92细胞对GPC3阳性的肝癌细胞Hep G2和Huh 7细胞毒作用。结果去岩藻糖基化改造的GC33抗体保留了与野生型GC33抗体对GPC3相当的结合活性,其与FCGRⅢa的结合能力增强了7倍,增强天然杀伤细胞(natural killer cells,NK)对Hep G2和Huh 7细胞的细胞毒作用(antibody dependent cellular cytotoxicity,ADCC)。结论去岩藻糖基化增强了GC33抗体依赖的NK92细胞对肝癌细胞杀伤效果

乌蔹莓化学成分的初步探究

目的:系统性研究福建道地药材乌蔹莓的有效成分。方法:运用大孔树脂,Sephadex LH-20,ODS及正相硅胶柱等色谱手段对乌蔹莓Cayratia japonica的全草进行分离。结果:从中得到13个化合物,通过多种波谱数据分析鉴定化合物结构,确定其分别为木犀草素(1),芹菜素(2),柠檬酸三乙酯(3),吲哚-3-甲醛(4),秦皮乙素(5),邻苯二甲酸二乙基己酯(6),calendin(7),反式咖啡酸乙酯(8),木犀草素7-O-葡萄糖苷(9),5-hydroxy-3,4-dimethyl-5-pentyl-2(5H)-furanone(10),3,4-二羟基苯甲酸乙酯(11),圣草酚(12),胡萝卜苷(13)。结论:其中3～8及10～12均为首次从乌蔹莓中分离得到

Study on Rapid Propagation Technique of Leather Corals

[目的]为海洋资源开发和生态环境修复等提供有效途径。[方法]探索肉质软珊瑚的人工快速扩繁方法。[结果]肉质软珊瑚具有快速的修复能力,能很快在肉质层的伤口处形成愈伤组织。通过该方法成功实现了肉质软珊瑚的快速人工扩繁。[结论]肉质软珊瑚内可能含有许多活性物质,具备一定的药物开发潜力。[Objective] The research aimed to provide an effective approach for developing ocean resources and repairing the ecological environment.[Method] The artificial method of rapid propagation of leather coral was explored.[Result] Leather coral was one species of soft corals,which had the properties of rapid regeneration and could develop the callus over the open wound.[Conclusion] Leather corals contained many active substances,so it had some medicinal development potential.2012年海南省科学事业费项目; 国家科技支撑计划项目(2012-BAC18B04-4); 国家海洋公益专项(201405020-5); 海南省科学事业费项目(KYYS-2014-50

Electric-field-induced selective catalysis of single-molecule reaction

随着单分子电学检测技术的迅速发展，分子电子学的研究不再局限于分子电子学器件的构筑及其电学性质的测量，而且扩展到单分子尺度化学反应过程的探索。然而目前相关的研究仍然局限于理论计算方面，在单分子尺度上实时监测和调控化学反应的活性和选择性是化学领域的长期目标和挑战。针对这一挑战，洪文晶教授课题组与程俊教授课题组合作，自主研发了精密科学仪器，将单个有机分子定向连接在两个末端尺寸为原子级的电极之间，解决了化学反应中分子取向控制的问题.理论计算结果证实了定向电场可以有效地稳定化学反应的过渡态，从而降低反应能垒。该研究工作在化学化工学院洪文晶教授、程俊教授、能源材料化学协同创新中心（iChEM）刘俊扬副研究员的共同指导下完成，由硕士研究生黄晓艳、iChEM博士研究生唐淳、博士研究生李洁琼以及兰州大学的陈力川博士作为共同第一作者，化学化工学院师佳副教授、陈招斌高级工程师、夏海平教授和田中群教授，萨本栋微纳研究院杨扬副教授、环境与生态学院白敏冬教授以及兰州大学张浩力教授参与了研究工作的讨论并给予指导，博士后乐家波、博士研究生郑珏婷、张佩（已毕业）、李瑞豪、李晓慧也参与了研究工作。Oriented external electric fields (OEEFs) offer a unique chance to tune catalytic selectivity by orienting the alignment of the electric field along the axis of the activated bond for a specific chemical reaction; however, they remain a key experimental challenge. Here, we experimentally and theoretically investigated the OEEF-induced selective catalysis in a two-step cascade reaction of the Diels-Alder addition followed by an aromatization process. Characterized by the mechanically controllable break junction (MCBJ) technique in the nanogap and confirmed by nuclear magnetic resonance (NMR) in bottles, OEEFs are found to selectively catalyze the aromatization reaction by one order of magnitude owing to the alignment of the electric field on the reaction axis. Meanwhile, the Diels-Alder reaction remained unchanged since its reaction axis is orthogonal to the electric fields. This orientation-selective catalytic effect of OEEFs reveals that chemical reactions can be selectively manipulated through the elegant alignment between the electric fields and the reaction axis.This work was supported by the National Key R&D Program of China (2017YFA0204902), the National Natural Science Foundation of China (21722305, 21703188, 21673195, 21621091, 51733004, 51525303, and 91745103), the China Postdoctoral Science Foundation (2017M622060), and the Young Thousand Talents Project of China. 该工作得到国家自然科学基金委（21722305、21703188、21673195、51733004、51525303、91745103），国家重点研发计划课题（2017YFA0204902），中国博士后面上基金（2017M622060）的资助，以及固体表面物理化学国家重点实验室、醇醚酯化工清洁生产国家工程实验室、能源材料化学协同创新中心的支持

Selective dynamical imaging of interferometric data

Recent developments in very long baseline interferometry (VLBI) have made it possible for the Event Horizon Telescope (EHT) to resolve the innermost accretion flows of the largest supermassive black holes on the sky. The sparse nature of the EHT’s (u, v)-coverage presents a challenge when attempting to resolve highly time-variable sources. We demonstrate that the changing (u, v)-coverage of the EHT can contain regions of time over the course of a single observation that facilitate dynamical imaging. These optimal time regions typically have projected baseline distributions that are approximately angularly isotropic and radially homogeneous. We derive a metric of coverage quality based on baseline isotropy and density that is capable of ranking array configurations by their ability to produce accurate dynamical reconstructions. We compare this metric to existing metrics in the literature and investigate their utility by performing dynamical reconstructions on synthetic data from simulated EHT observations of sources with simple orbital variability. We then use these results to make recommendations for imaging the 2017 EHT Sgr A* data sethttp://iopscience.iop.org/2041-8205Physic